Objective To evaluate the clinical value of bone imaging in diagnosing bone metastasis from lung cancer. Methods A total of 201 patients with diagnosed lung cancer were examined by ~(99)Tc~(m)-MDP bone imaging,and the bone metastatic sites,quantity and pathological types of those with skeletal metastases were reviewed and analyzed.Results Among these 201 patients,134 cases were found with skeletal metastases,commonly in the thoracic bone,the sinal and pelvic bone.Lung cancer was identified easier to have the skeletal metastases of adenocarcinoma than other types of cancers.The positive rate of bone metastasis diagnosed by radionuclide bone imaging was rather higher than that of X-ray and CT.Conclusion Radionuclide bone imaging should be routinely performed in patients with lung cancer.

The article opened out the meaning which constructed the talents quality standard from the connotation of"quality"and put forward the talent quality standard which is opening,multiple and keeping pace with the development of pharmaceutical section,TCM modernization and the college's future so as to meet the society's demands for skilled talents.

An improved method for the purification of 1,25-(OH)2D3-26,23-lactone was established by using one step of highperformance liquid chromatography (HPLC). The analytic column was Shim-pack NH2 (straight-phase type). The mobile phase was Methanol: Dichloromethane: Hexane = 4:26:70 and the flow rate was 2ml/min. An ultraviolet detector with 254nm was used. The retention time of 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1, 24, 25-(OH)3D3 werel2.10, 17.06 and 22,00 minutes respectively. This method can separate 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1,24,25-(OH)3D3 only by one step of HPLC and it is better than the old one which need two steps of HPLC for the separation of these substances.

Objective To observe the expression of IGF-1 in the peri-hematomal brain region of Intracerebral Hemorrhage(ICH) patients,and to investigate the relationship among IGF-1,excessive and depletion syndrome of stroke.Methods 28 patients were evenly and randomly recruited into 3groups:Yin excessive group,Yang excessive group and depletion group.Additional 6 persons of normal death within 3 hours were taken their brain tissue.The IGF-1 expression was detected with Western blotting method.The correlation among the expression of IGF-1,excessive and depletion syndromes were observed.Results The expression of IGF-1 in the peri-hematomal brain region of ICH patients significantly increased in comparison with the normal control group (P=0.00).The expression of IGF-1 was significantly different among Yang excessive,Yin excessive and the depletion syndromes [ (0.696±0.111)、(1.151 ±0.185)、(1.697±0.108),P＜0.01],with the expression of IGF-1 of depletion syndrome ranked the highest,followed by Yin excessive and Yang excessive syndrome.Conclusion The expression of IGF-1 could reflect the degree of brain impairment.

Objective To investigate the application of CUSUM (cumulative sum control chart) curve in cardiac clinical teaching of resident surgeon education.Methods We chose a surgeon of 10years work experience of attending the preparation operation of internal mammary artery as control reference (standard group,28 patients) and a resident engaged in professional clinical cardiac work no more than 5 years as group 1,a professional training physician engaged in cardiac surgery clinical work more than 5 years as group 2 and took 40 patients' data respectively from the two groups' preparation of internal mammary artery operation.And then we recorded the postoperative mammary artery flow,internal mammary artery preparation time and adverse events data and made statistics analysis with SPSS 19.0,and CUSUM curve with Matlab R2012b polynomial curve.Results When the density of operate interval and the operate numbers came to about 25 cases,the polynomial eurve's slope rate went up to 0,regardless the surgeons' experience,CUSUM value was accumulated from 66 to 76,and overcame the learning curve successfully.However,when the density of operate interval did no reach the standard,the surgeon's CUSUM ploynomial cmwe slope rate never reached to 0,and can't overcome the learning curve.Conclusion CUSUM curve gives a clear quantitative indicator to the cultivation of clinical physician,and makes teaching teachers can understand the young physicians' learning process,so as to change the teaching plan,help young physicians cross the learning curve as soon as possible.

This paper summarizes the nursing care of a child with hand,food and mouth disease complicating multiple organ failure,such as use of ice-blanket and strategies of vessel protection. As a result,the child recovered well and was discharged after rehabilitation.

Objective To investigate the influence of co-expression state of p14 ARF and p16 INK4a protein on radiochemotherapy and length of survival period in patients with non-small cell lung cancer(NSCLC)after resection. Methods Thirty-five patients with negative p14 ARF and p16 INK4a co-expression and 20 patients with positive p14 ARF and p16 INK4a co-expression were enrolled for the study. The co-expression of the said proteins were previously determined by immunohistochemistry (S-P). Clinical pathological characteristics were compared between two groups, and the survival time and the results of radiochemotherapy of patients were respectively recorded and analysed. Results No significant differences were found in age, TNM stages, degree of differentiation, recurrence/metastasis and radiochemotherapy between two groups. However, there was significant differences in sex, smoking index, and pathological classification. It was found that 2-year, 3-year and total survival rate were significantly lower in patients with p14 ARF and p16 INK4a co-expression than those with positive co-expression (P

Objective To investigate the effect of gene GCRG213 transfection (sense, anti-sense) on growth of gastric cancer cell MKN45. Methods The sense and anti-sense fragment of GCRG213 were obtained by PCR. They were cloned into eukaryotic expression vector pcDNA3.1(+). The recombinant plasmid pcDNA3.1-a, pcDNA3.1-b and the vector pcDNA3.1 were transfected separately into MKN45 cells conducted by lipofectamine~ TM 2000. Expression of GCRG213 was assessed with semi-quantitative RT-PCR and Western Blot. The growth graph was plotted by the methods of cell counting of three steady transfected cells. FACS was used to determine the cell cycle, and Annexin V FITC/PI bi-labeling method was used to determine the cell apoptosis. Results By sequencing, sense GCRG213 and anti-sense GCRG213 were proved to be successfully cloned into pcDNA3.1. Transfecting the sense vector (pcDNA3.1-a) into the MKN45 significantly increased the expression of GCRG213, both in mRNA level and protein level. Transfecting the anti-sense vector (pcDNA3.1-b) into the MKN45 significantly decreased the expression of GCRG213, both in mRNA level and protein level. The growth of pcDNA3.1-a transfected cells was faster than that of vector transfected cells, and the cell apoptosis decreased. But the growth of pcDNA3.1-b transfected cells was slower in multiplication than that of vector transfected cells, and the cell apoptosis was increased. Conclusion Stable transfection showed that GCRG213 promoted cell to grow, inhibited the cell apoptosis. GCRG213 might be a new promoter to tumor.

To observe the protective e ect of oral Liuweidihuang pill combined with Shengmai capsule on heart of primary hypertension with syndrome of de ciency of both qi and yin of heart and kidney.Method:While decreasing blood pressure with Captopril,oral Liuweidihuang pill combined with Shengmai capsule was used to treat 55 cases of primary hypertension,control groups was established.BP,cardiac function,damage degree of myocardial hypertrophy and blood vessel endothelium were detected.Result:ALL the indexes above in treatment group decreased obviously,showed signi cant di erence when compared with control group.Conclusion:Oral Liuweidihuang pill combined with Shengmai capsule and Captopril has cooperative e ect of antihypertension.The combined medication can treat heart damage by reducing myocardial oxygen consumption index,reversing myocardial hypertrophy,improving left ventricular function,protecting blood vessel endothelium,and preventing arteriosclerosis.

Along with the rising of Chinese medicine consumption,health food with Chinese medical feature plays an increasingly role in people's life,and its market shares increase every year.This article analyzes the advantages from aspects of literature,theory,function,security,resources and market of health food,in order to develop health food and inherit the theory of Chinese medicine.