BACKGROUND:Basic fibroblast growth factor (bFGF) can enhance fibroblast proliferation and col agen deposition, and vascular endothelial growth factor (VEGF) can improve blood perfusion and metabolic level of pathological tissues. Additional y, both of them can boost the alkaline phosphatase activity under given conditions. OBJECTIVE:To evaluate the effect of bFGF combined with VEGF on the periodontal ligament fibroblast proliferation and alkaline phosphatase activity in rats. METHODS:Rat periodontal ligament fibroblasts were cultured in vitro, its embryonic origin was identified and passage 4 cel s were used for the fol owing experiments. Effects of bFGF and VEGF with different concentrations on the rat periodontal ligament fibroblast proliferation were detected to determine the minimum and maximum effective concentrations. Cel s were divided into five groups:group A (control group) with DMEM containing 2%fetal bovine serum;group B as maximum effective concentration of VEGF group;group C as maximum effective concentration of bFGF;group D as minimum effective concentration of bFGF combined with minimum effective concentration of VEGF group;group E as maximum effective concentration of bFGF combined with maximum effective concentration of VEGF group. At 3, 7 and 14 days, the alkaline phosphatase activity in each group was detected. RESULTS AND CONCLUSION:Rat periodontal ligament fibroblasts derived from the mesoderm grew wel . Rat periodontal ligament fibroblast proliferation was increased with the VEGF and bFGF concentration increasing (P<0.01). The maximum and minimum effective concentrations of VEGF were 100 and 10μg/L, and the maximum and minimum effective concentrations of bFGF were 10 and 0.1μg/L. The absorbance values in the groups D and E were higher than those in the group A. The absorbance values of the group D were significantly lower than those of the group E at 3 and 7 days (P<0.05), but did not significantly differ at 14 days (P>0.05). To conclude, the combination use of the maximum effective concentration of VEGF and bFGF can play a significant synergistic effect on the alkaline phosphatase activity at a given time, but the minimum and maximum effective concentrations show no significant differences if not in the given time, which may be related to the time-effectiveness of these two factors and the receptors of periodontal ligament cel s. Subject headings:Fibroblast Growth Factor 2;Vascular Endothelial Growth Factors;Alkaline Phosphatase;Tissue Engineering

Asthma,a chronic inflammatory airway disease,is influenced by both genetic and environmental factors.It is a complex disease that involves the interplay among multiple physiological processes.Currently,it has been identified that 17q21 loci genes especially orosomucoid like 3 (ORMDL3) and gasdermin B (GSMDB) are strongly linked with the susceptibility and severity of childhood asthma by using of the Genome-Wide Association Study (GWAS).Furthermore,a better understanding of the molecular mechanism contributes to the asthma target therapeutics and precision medicine.This review summarizes the 17q21 loci genes associated with the susceptibility,severity,and race specificities of childhood asthma.

Cervical cancer active targeting nano drug delivery system delivers drug-loaded nanoparticles to cancer cells in a targeted way through specific ligand-receptor interaction, which has the advantages of reducing adverse drug reactions and improving drug efficacy. It is of great significance to understand the active targeting nano drug delivery system for cervical cancer to explore new carriers, drugs and targets.

Objective To explore the mechanism of combined treatment with methotrexate (MTX) and Ga-Al-As laser irradiation for rheumatoid arthritis (RA) and to assess the effectiveness of Ga-Al-As laser therapy for RA. Methods Twenty-two patients with RA were randomly and evenly divided into two groups: the treatment group treated with Ga-Al-As laser irradiation combined with MTX and the control group treated with MTX only. Ten age-matched normal subjects were observed as normal controls. The amount of CD4 + CD25 + regulatory T cells in peripheral blood (PB) of the normal controls and that in the PB and synovial fluid (SF) of the 22 patients before and after therapy were counted by flow cytometry. Meanwhile, the amount of prostaglandin E2 (PGE2) in synovial fluid of the patients was measured before and after treatment by enzyme-linked immunosorbent assay(ELISA). Results After combined treatment the clinical symptoms of the patients were improved significantly, and the amount of PGE2in SF decreased significantly. The count of CD4 + CD25 + regulatory T cells in PB of RA patients was ( 3.84 ±3.20) % , compared to ( 10.05 ± 7.04) % in healthy individuals. The count of CD4 + CD25 + regulatory T cells in SF of RA patients was ( 14.89 ± 12.30) % , much higher than that in PB. The count of CD4 + CD25 + regulatory T cells in SF decreased significantly in treatment group compared to control group (P ＜0.05). Conclusion Ga-Al-As laser irradiation eombined with MTX can effectively improve the clinical symptoms of RA patients. It may be related to the decrease of amount of PGE2 and count of CD4 + CD25 + regulatory T cell in PB and SF.

Objective To evaluate the effect of long-term and low-dose aspirin therapy on the severity of acute ischemic stroke. Method 321 acute ischemic stroke patients were randomly divided into two groups: aspirin group and Non-aspirin group. In addition, a pair study was made.Clinical scores were used to indicate the severity of acute ischemic stroke. Results Clinical scores showed that there were no significant differences statistically in the two random groups and pair study. Conclusions Long-term and low-dose aspirin therapy can not reduce the severity of acute ischemic stroke.

Objective:To investigate the expression change of voltage-gated calcium channels(VGCC) in the facial nucleus motoneurons of adult rats after facial nerve injury.Methods:The facial motor nucleus was localized by retrograde labeling with a fluorescent dye,Dil,and identified by Nissl staining.The facial nerve injury model was established by amputation of the main trunk of left facial nerve.Exposure of the right facial nerve without nerve transection was used as the control.Rats were sacrificed at 3,7,14 and 28 days after injury respectively(n =10),the brainstem containing facial nucleus were collected,the expression of P/Q,N,L,R-type calcium channel α1A,α1B,α1C and α1E subunits was examined by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR).Results:Immunohistochemistry results show that whereas α1A,α1E subunits levels did not vary compared with control group 3,7,14,28 days after injury(P ＞0.05),α1B and α1C subunits immunoreactivity decreased in the motoneurons after injury,a sharp decrease was detected at 14 days after injury(P ＜ 0.01),thereafter returned to the control level at 28 days after axotomy(P ＞0.05).The expression of α1B and α1C mRNA was down-regulated,especially 14 days after the injury(P ＜0.01),and then recovered to normal level at 28 days (P ＞ 0.05).In addition,there was no significant difference of α1A and α1E subunits and their correspoding mRNA between operated group and control group at all time points(P ＞ 0.05).Conclusion:VGCC is involved in facial nerve injury and down-regulation of N-type and L-type calcium channels may be one of the role.

OBJECTIVE To explore the rational antibiotics usage in hepato-biliary department.METHODS The antibiotics used in liver surgery department were investigated and evaluated according to the standards.RESULTS Totally 235 patients (88.01%) among 267 patients from 2005 to 2007 were used antibiotics,of which 18.30% for therapy,81.70% for prophylaxis and from them 83.40% were for systemic use. The specimens from 35 patients among 235 patients with liver disease,were sent to do antibiotic susceptibility test. Most of the patiants were given therapy by experience.CONCLUSIONS The administration department in the hospital should enhance its work by forming institutions and standards,and inspecting regularly to guarantee the safety of antibiotics usage.

Objective To investigate the common allergens and the relationship between the common allergens and the age of children with bronchial asthma in Jiading area of Shanghai. Methods Skin prick test (SPT) of 15 common allergens with standard prick liquid were performed in 351 asthmatic children, the children were divided into ≤3 years group, 3 ～6 years group and ＞6 years group, and we observed the positive rates and allergens in different age groups. Results (1) The positive rate of SPT for inhalant allergens in children with bronchial asthma is 71. 2%, specifically are: dust mite (49. 6%), house dust mite (49. 0%), fungi Ⅰ (36. 8%), mold Ⅱ (34. 8%), tree Ⅰ (32. 5%), dog epithelium (31.9%), cat epithelium (31.3%), pollen (29. 1%), tree Ⅱ (28. 8%), and feather (27.4%). (2)The positive rate of SPT for food allergens in children with bronchial asthma is 39. 6%, specifically are: shrimp(24.2%), eggs(15.7%), milk(14.8%), peanuts (13. 7%), and curries(12.5%). (3) The positive rate of inhalant allergens was significantly higher than food allergens. The positive rate of inhalant allergens had no significant differences among different ages, while the positive rate of food allergens was increased with age. Conclusions Most children with bronchial asthma and allergens are related, and allergens are related to age.

Objective To investigate the features of vascular damage in metabolic syndrome (MS) by a comparison of changes in vascular structure and function between spontaneous hypertension (SH) and MS in rats. Methods Pathological study of changes in arteries was carried out. The contractile response of aorta ring and the expression of RhoA, ROCK, eNOS and iNOS were also determined. Results Besides the thickening of arterial wall and proliferation of both intima and smooth muscle layer, marked infiltration of inflammatory cell appeared in large and medium arteries of MS rats, and obvious hyaline degeneration was found in mesenteric arterioles. Moreover, the relaxant response of aorta ring was weakened markedly in MS rats, while the contractile response increased in SH rats dramatically. The expression of RhoA, ROCK increased and that of NOS decreased in MS rats, but the up-regulation of the RhoA, ROCK expression was not as much as in SH rats. Conclusion The damage to both arterial structure and function was much more serious in MS rats than that in SH rats. Activation of RhoA/ROCK system and reduction of NOS expression might be involved in the mechanism.

Objective To study the signal transduction mechanism of nicotine induced periodontal ligament fibroblasts (PDLFs) apoptosis. Methods This study used 1 μg/ml, 10μg/ml and 100μg/ml nicotine to intervene PDLFs cells for 24h separately. NF-κB, p53, I-κB and Caspase3 expression were detected. Results After Nicotine was done on PDLFs cells for 24h, the transcription of p53, and Caspase3,and the translation of Caspase 3 protein were increased, while NF-κB was decreased. At the same time, the transcription of NF-κB decreased gradually with the concentration of nicotine increased ( r = 0. 707, F =33. 705, P ＜0. 01 ), nevertheless, I-κB was reversed ( r =0. 964, F =374. 883, P ＜0. 01 ). p53 expression was increased gradually with the concentration of nicotine increased ( r =0. 957, F = 153. 377, P ＜0. 01).Both Caspase3 mRNA (r =0.935, F =318.371, P ＜0.01) and protein (r =0.677, F =8. 459, P ＜ 0. 05 )increased gradually. Conclusion Nicotine induced PDLFs apoptosis was mediated through NF-κB and p53 pathway.