Objective To investigate the different ability to produce H_(2)O_(2+) of the vaginal lactobacilli and their potential antagonism toward E.coli in ivtro.Then to select the potential lactobacilli strain to treat the female urogenital infection.Method To detect production of H_(2)O_(2) by measuring the blue matter in water solution with absorbtion spectrophotometry.The lactobacillus and E.coli were cultured together in solid culture medium,followed by the measurement of inhibition zone around the lactobacilli after 24 hour.Results We selected 4 strains of lactobacilli from vagina.L.acidophilus1 and L.paracasei3 are the stronger to produce H_(2)O_(2).The largest diameter of the ring of inhibition is shown around the lactobacilli acidophilus 1.Conclusion The lactobacilli acidophilus 1 and lactobacilli crispatus are the most potential strains as antagonist to E.coli and their antagonism depend not only on lacid or H_(2)O_(2).

Polo-like kinase 1 (PLK1) is a highly conserved serine/threonine protein kinase that has attracted research attention be-cause it plays a critical role in mitosis regulation. PLK1 is overexpressed in 80%of human tumors, which indicates a poor prognosis in most tumors. PLK1 is one of the most promising targets for antitumor therapy because it is upregulated in castrate-resistant prostate can-cer (CRPC). This review focused on the basic structure and function of PLK1, the relationship between PLK1 and CRPC occurrence and progression, and CRPC treatment by inhibiting PLK1. This study provides a theoretical basis for the targeted molecular therapy of CRPC.

To investigate the psychological difference in abdominal aortic aneurysm patients before and after operation and its influence on life quality, 24 patients who can understand and complete the questionnaire were enrolled in the study, the inquiry was performed after admission and discharge with the standard SCL 90 table. The results showed that the general conditions, depression, anxiety, compulsive symptoms and fear of the patients had marked differences before and after operation. It is concluded that operation of abdominal aortic aneurysm can not only prevent the rupture of aneurysm but also relieve the patients′ psychological pressuer and improve their life quality.

Objective:To study the mechanism on extracellular signal-regulate kinases ( ERK) signal transduction by calcium influx initiated by combination of estrogen with calcium channels or estrogen re-ceptor in endometrial cancer cell Ishikawa. Methods: Confocal test was used to determine the relative calcium mobilization by stimulation of estrodiol together with and without the inhibition of ICI182780 and nifedipine. Western-blotting was used to detect the protein expression of phosphorylated ERK1/2 (P-ERK1/2) in the same condition. Results:The transient calcium flux initiated by 17β-estrodiol (E2) and a membrane-impermeable conjugate of estrogen and bovine serum albumin ( E2-BSA ) , and the calcium mobilization could be inhibited by ICI182780 and nifedipine in 1 min. In Ishikawa cells, phosphorylation of ERK1/2 was stimulated by E2 , and the phosphorylation could not be inhibited by E2 after the combination with ICI182780 in 5 min and in 30 min. The phosphorylation also could not be in-hibited by E2-BSA after the combination with nifedipine in 5 min, but in 30 min the phosphorylation was decreased. The phosphorylation of ERK by E2-BSA was decreased by the combination with nifedipine in 30 min. Conclusion:The transient calcium flux initiated by estrogen has an effect on the activation of ERK signal pathway in endometrial carcinoma cells.

BACKGROUND: It is well known that central noradrenergic system takes part in learning and memory, but there are a few researches focused on the effects of impairing the central noradrenergic system on rat' s P3-like cognitive potential.OBJECTIVE: To study the effects of impairing the central noradrenergic system on rat's P3-like cognitive potential.DESIGN: Randomized controlled study based on the experimental animals.SETTING: Department of physiology in a medical college of a university.MATERIALS: Totally 30 male SD rats, clean degree, weighing from 500 to 600 g, were selected from the Experimental Animal Center of Sun Yat-sen Medical University(certificate: 26-99A018), drank freely and fed timely under the normal condition. After Y-maze test of all the rats, 24 rats with normal learning and memory function, which met the criteria specified in the description of Y-maze test, were randomly divided into three groups(8 rats in each group): control group, 6-oxidopamine (6-OHDA) injection group and saline control group.INTERVENTIONS: The models of central norndrenergic system impairment were made by injecting 6-hydroxysopamine(6-OHDA) into the bilateral dorsal noradrenergic bundle in dentate granule(DG). Then Y-type maze test and elicitation of P3-likewere carried out respectively before and after the models were built.MAIN OUTCOME MEASURES: Main outcome: P3-like latency, total reaction time(TRT) and error number(EN). Secondary outcome: Results of P3-like test recorded in the 12 days after the models were built.RESULTS: As compared with the controls, the model rats' P3-like latency and total reaction time(TRT) prolonged and error number(EN) increased statistically ( P ＜ 0.05 ).CONCLUSION: Central NE takes part in the activities of learning and memory and plays a certain role in the production and integration of P3 -like.

Objective and accurate assessment of liver fibrosis in patients with chronic hepatitis B (CHB)helps to choose the correct therapeu-tic scheme and guide the clinical treatment.In recent years,the noninvasive diagnostic models for liver fibrosis have been constantly emerging, but no consensus has been reached in this regard.The research advances in noninvasive regression models for liver fibrosis in patients with CHB are reviewed according to different staging methods for liver fibrosis,and the existing models are analyzed in terms of problems and shortages. It is thought that further studies should be done to establish simpler,more effective models that have higher diagnostic values.

Objective To explore the impact of resilience and anxiety, depression on quality of life (QOL) among esophageal carcinoma patients undergoing radiotherapy and (or) chemotherapy. Methods A total of 125 patients were recruited into our study. Date were collected by European Organization for Research and Treatment of Cancer, Quality of Life Questionnaire-Core 30 (EORTC QLQ-C30) , Resilience Scale (RS) and Hospital Anxiety and Depression Scale (HADS). Analysis of Moment Structures (AMOS) version 7.0 software was used to conduct the path analysis. Results The total score of patients′ QOL, RS and HADS were(51.98±19.42)points,(56.32±17.09)points,(14.59±7.04)points. Pearson′s analysis showed that QOL was correlated with RS and HADS ( r =0.423-0.588,P<0.05). RS was negatively correlated with HADS (path coefficient was-0.76 and-0.37) and HADS negatively correlated with QOL (path coefficient was-0.56 and-0.79). RS as a protective factor indirectly influenced QOL via HADS and the paths′ total coefficients was 0.69. Conclusions The level of QOL and RS were at poor level for esophageal carcinoma patients, as well as participants experienced anxiety and depression. HADS as intermediary variable has a greater moderating effect on relationships between RS and QOL. RS is a indirect protective factor of QOL. Interventions developed on the basis of influencing factors of QOL should be applied to improve patients′integral health status.

[Summary] Epithelial ovarian carcinoma has the highest fatality rate among gynecologic malignant tumors.Lacking of effective early diagnosis and screening method are the main reasons for its poor prognosis.There is a close relationship between the heterogeneity of epithelial ovarian carcinoma and the diversity of cancerous origins.According to the traditional theory, epithelial ovarian carcinoma derives from germinal epithelium at ovarian surface.The “secondary Mullerian system” theory has challenged above view.This“dualism” theory completely subverts the traditional one.However, both of the theories have their limitations.In recent years, the multiple origins theory of epithelial ovarian carcinoma has gradually become a hot point of discussion.

Objective To evaluate changes and clinical significance of products of hurted endothelium in pregnant women with hypertensive disorder.Methods The levels of tissue factor(TF),tissue-type plasminogen activator(tPA),plasminogen activator inhibitor(PAI),von Willebrand factor(vWF) were determined in 87 pregnant women with hypertension as well as in 100 normal pregnant women and 100 normal nonpregnant women.Results The levels of TF,PAI and vWF were higher in pregnant women with hypertension than in normal pregnant women and nonpregnant women(P0.05),but the contents of tPA in these two groups were significantly higher than that in normal nonpregnant women(P

BACKGROUND:Basic fibroblast growth factor (bFGF) can enhance fibroblast proliferation and col agen deposition, and vascular endothelial growth factor (VEGF) can improve blood perfusion and metabolic level of pathological tissues. Additional y, both of them can boost the alkaline phosphatase activity under given conditions. OBJECTIVE:To evaluate the effect of bFGF combined with VEGF on the periodontal ligament fibroblast proliferation and alkaline phosphatase activity in rats. METHODS:Rat periodontal ligament fibroblasts were cultured in vitro, its embryonic origin was identified and passage 4 cel s were used for the fol owing experiments. Effects of bFGF and VEGF with different concentrations on the rat periodontal ligament fibroblast proliferation were detected to determine the minimum and maximum effective concentrations. Cel s were divided into five groups:group A (control group) with DMEM containing 2%fetal bovine serum;group B as maximum effective concentration of VEGF group;group C as maximum effective concentration of bFGF;group D as minimum effective concentration of bFGF combined with minimum effective concentration of VEGF group;group E as maximum effective concentration of bFGF combined with maximum effective concentration of VEGF group. At 3, 7 and 14 days, the alkaline phosphatase activity in each group was detected. RESULTS AND CONCLUSION:Rat periodontal ligament fibroblasts derived from the mesoderm grew wel . Rat periodontal ligament fibroblast proliferation was increased with the VEGF and bFGF concentration increasing (P<0.01). The maximum and minimum effective concentrations of VEGF were 100 and 10μg/L, and the maximum and minimum effective concentrations of bFGF were 10 and 0.1μg/L. The absorbance values in the groups D and E were higher than those in the group A. The absorbance values of the group D were significantly lower than those of the group E at 3 and 7 days (P<0.05), but did not significantly differ at 14 days (P>0.05). To conclude, the combination use of the maximum effective concentration of VEGF and bFGF can play a significant synergistic effect on the alkaline phosphatase activity at a given time, but the minimum and maximum effective concentrations show no significant differences if not in the given time, which may be related to the time-effectiveness of these two factors and the receptors of periodontal ligament cel s. Subject headings:Fibroblast Growth Factor 2;Vascular Endothelial Growth Factors;Alkaline Phosphatase;Tissue Engineering