Objective To investigate the different ability to produce H_(2)O_(2+) of the vaginal lactobacilli and their potential antagonism toward E.coli in ivtro.Then to select the potential lactobacilli strain to treat the female urogenital infection.Method To detect production of H_(2)O_(2) by measuring the blue matter in water solution with absorbtion spectrophotometry.The lactobacillus and E.coli were cultured together in solid culture medium,followed by the measurement of inhibition zone around the lactobacilli after 24 hour.Results We selected 4 strains of lactobacilli from vagina.L.acidophilus1 and L.paracasei3 are the stronger to produce H_(2)O_(2).The largest diameter of the ring of inhibition is shown around the lactobacilli acidophilus 1.Conclusion The lactobacilli acidophilus 1 and lactobacilli crispatus are the most potential strains as antagonist to E.coli and their antagonism depend not only on lacid or H_(2)O_(2).

Polo-like kinase 1 (PLK1) is a highly conserved serine/threonine protein kinase that has attracted research attention be-cause it plays a critical role in mitosis regulation. PLK1 is overexpressed in 80%of human tumors, which indicates a poor prognosis in most tumors. PLK1 is one of the most promising targets for antitumor therapy because it is upregulated in castrate-resistant prostate can-cer (CRPC). This review focused on the basic structure and function of PLK1, the relationship between PLK1 and CRPC occurrence and progression, and CRPC treatment by inhibiting PLK1. This study provides a theoretical basis for the targeted molecular therapy of CRPC.

To investigate the psychological difference in abdominal aortic aneurysm patients before and after operation and its influence on life quality, 24 patients who can understand and complete the questionnaire were enrolled in the study, the inquiry was performed after admission and discharge with the standard SCL 90 table. The results showed that the general conditions, depression, anxiety, compulsive symptoms and fear of the patients had marked differences before and after operation. It is concluded that operation of abdominal aortic aneurysm can not only prevent the rupture of aneurysm but also relieve the patients′ psychological pressuer and improve their life quality.

Objective To explore the impact of resilience and anxiety, depression on quality of life (QOL) among esophageal carcinoma patients undergoing radiotherapy and (or) chemotherapy. Methods A total of 125 patients were recruited into our study. Date were collected by European Organization for Research and Treatment of Cancer, Quality of Life Questionnaire-Core 30 (EORTC QLQ-C30) , Resilience Scale (RS) and Hospital Anxiety and Depression Scale (HADS). Analysis of Moment Structures (AMOS) version 7.0 software was used to conduct the path analysis. Results The total score of patients′ QOL, RS and HADS were(51.98±19.42)points,(56.32±17.09)points,(14.59±7.04)points. Pearson′s analysis showed that QOL was correlated with RS and HADS ( r =0.423-0.588,P<0.05). RS was negatively correlated with HADS (path coefficient was-0.76 and-0.37) and HADS negatively correlated with QOL (path coefficient was-0.56 and-0.79). RS as a protective factor indirectly influenced QOL via HADS and the paths′ total coefficients was 0.69. Conclusions The level of QOL and RS were at poor level for esophageal carcinoma patients, as well as participants experienced anxiety and depression. HADS as intermediary variable has a greater moderating effect on relationships between RS and QOL. RS is a indirect protective factor of QOL. Interventions developed on the basis of influencing factors of QOL should be applied to improve patients′integral health status.

Objective and accurate assessment of liver fibrosis in patients with chronic hepatitis B (CHB)helps to choose the correct therapeu-tic scheme and guide the clinical treatment.In recent years,the noninvasive diagnostic models for liver fibrosis have been constantly emerging, but no consensus has been reached in this regard.The research advances in noninvasive regression models for liver fibrosis in patients with CHB are reviewed according to different staging methods for liver fibrosis,and the existing models are analyzed in terms of problems and shortages. It is thought that further studies should be done to establish simpler,more effective models that have higher diagnostic values.

Objective To investigate the effect of Zusanli(ST36) moxibustion on facial infrared thermal images in infantile spasm patients and provide a clinical basis for rehabilitation treatment of infantile spasm.Method Fifteen infantile spasm patients were enrolled. Ten healthy children constituted a normal control group. Facial infrared thermal images were observed in the infantile spasm patients. An auto-control study was also conducted in the infantile spasm patients. The infrared thermal images before and after 15 minutes of a rest state were used as a blank group and those before and after 15 minutes of moxibustion, as a treatment group. Facial infrared thermal images were produce using anATIR-M301 medical far-infrared thermograph. Changes in temperature in facial regions were observed in the control and treatment groups. A statistical analysis was carried out.Result Facial infrared thermal images showed that bilateral temperatures were evenly and symmetrically distributed in the normal group and asymmetrically distributed, with the right side temperature lower than the left side, in the spasm group of patients. There were statistically significant differences in the forehead and inner canthus(P<0.05). There was no statistically significant difference in the facial thermal image (temperature) between before and after 15 minutes in the blank group (P>0.05). In the treatment group, facial thermal images tended to be symmetrical after 15 minutes of moxibustion and had a statistically significant difference between before and after the moxibustion (P<0.05).Conclusion Zusanli moxibustion can improve facial blood circulation in infantile spasm patients.

Objective To determine whether platelet activation is associated with active ulcerative colitis (UC). Methods Platelet aggregability was assessed by means of SH 93 intelligent blood aggregation and coagulation tester; P selectin and thromboxane B 2 (TXB 2) were detected by means of ELISA method in patients with UC as well as in controls, including healthy volunteers and patients with irritable bowel syndrome (IBS). Colonoscopy and biopsy were performed in 43 patients with UC. Results Increased circulating platelet aggregability was detected in active ulcerative colitis compared with IBS and healthy controls ( P

ObjectiveTo investigate the causes, operation time, and microscopic repairing procedures of contusive iridodialysis. Methods23cases(23 eyes) who had partial iridodialysis due to contusions of the eyeballs underwent the microscopic repair during 7 to 18 days after injury. Viscoelastic agent and other auxiliary devices were used in the operation. ResultsAll eyes were fully corrected with approximate round-shaped pupils and their visual function improved. 1 eye with raised intraocular pressure was performed with trabeculectomy. ConclusionTimely repair of partial iridodialysis performed under microscope was safe and effective.

Objective To investigate influences of paeonol on mRNA expression and function of drug transporters BCRP and SLCO1B1 in HepG2 cell.Methods Cell counting Kit-8 assay was used to detect the viability of HepG2 cells;Real-time fluorescent quantitative PCR (qPCR) was performed to measure the expressions of BCRP and SLCO1B1 mRNAs; flow cytometry was applied to determine the transport functions of BCRP and SLCO1B1. Results Paeonol (2-8μg/mL) did not decrease HepG2 cell survival rate, but 16 μg/mL paeonol significantly reduced cell survival rate (P<0.05). Paeonol(2-8μg/mL)significantly induced the mRNA expression and function of drug transporters BCRP and SLCO1B1(P <0.05).Compared with control group, transcription level of paeonol group’s BCRP and SLCO1B1 drug transporters obviously up-regulated, the of translocation efficiency of substrate specificity increased significantly (P <0.05).Conclusion Paeonol can induce drug hepatocellular transporters BCRP and SLCO1B1 gene expression, thereby promote the substrate transport the transmembrane.It is indicated that the drug combination of paeonol and BCRP and SLCO1B1 transporters, there may be a risk of drug interactions.

Objective To establish a quick method to probe the environmental estrogen-like compounds via fluorescent imaging analysis in high content analysis (HCA) technology.Methods HCA assays were performed to quantitatively in-vestigate the effect of collected environmental pollutants , including bisphenol A , nonylphenol , 1,2-phenylenediamine , 4-aminophenol, resorcinol, 3-aminophenol, 1,4-phenylenediamine and 2,5-diaminotoluene on nuclear granules formation ( Foci-formation) of estrogen receptor α( ERα)-EGFR( enhanced green fluorescent protein ) fusion-protein, which was se-lected as a research model in this study .The results were confirmed by the ERαtranscriptional activity by luciferase as-says.Results Compounds 1,2-phenylenediamine, 4-aminophenol, resorcinol, 3-aminophenol, 1,4-phenylenediamine and 2,5-diaminotoluene sulfate could not induce the ERα-EGFR nuclear granule formation.17-β-Estradiol, bisphenol A, or nonylphenol enhanced ERα-EGFR nuclear granule formation in a dose-dependent manner .The EC50 value was (4.17 ± 0.41) nmol/L, (1.48 ±1.79) μmol/L,or (3.70 ±0.78) μmol/L, respectively.The minimum detectable concentration was 1 nmol/L (17-β-estradiol)and 300 nmol/L (bisphenol A, nonylphenol).In luciferase tests, 17-β-estradiol, bisphe-nol A, or nonylphenol increased ERαtranscriptional activity in a dose-dependent manner ,and the EC50 value was (4.46 ±0.56) nmol/L, (2.31 ±0.21) μmol/L, or (6.60 ±0.94) μmol/L, respectively.The minimum detectable concentration was 3 nmol/L (17-β-estradiol), 300 nmol/L (bisphenol A),and 1 μmol/L (nonylphenol).Conclusion As an efficient method for ERαagonist identification , HCA assays based on the cell image phenotypes analysis can be used in quick recog -nition of environmental compounds with ER agonist-like activity.In all experimental compounds , only bisphenol A and non-ylphenol have a clear ER agonist-like activity .