0.05). In comparison with the control group,the levels of FPG and GSP were significantly higher (P

MicroRNAs (miRNAs) negatively mediate the post transcriptional target genes by degrading mRNAs and inhibiting translation of protein.Deregulation of miRNAs are directly or indirectly correlated with tumorigenesis and development of cancer.Currently,the application of miRNAs as a non-invasive potential biomarker with high stability is under investigation.

Objective To study the action of GSK-3βin podocyte transdifferentiation effecundethe high glucose condition . MethodPodocytewere treated in RPMI-1640 medium with differenconcentrationof glucose fo36 h .The expressionof neph-rin ,podocin ,α-Smand Fibronectin were detected by both Western bloand indirecimmunofluorescence analysi.Athe same time ,the change of albumin inflow amounof podocytein differentreatmengroupwadetected by using the Transwell cham-be.The changeof expression amounand activity of GSK-3βin high glucose condition were detected by using the GSK-3 activity assay ki.The phenotypiand functional changeof podocytewere detected aftedisturbing GSK-3βby GSK-3βsiRNin the high glucose group .ResultThe expression levelof nephrin and podocin protein were down-regulated with the increase of the glucose concentration in dose-dependenmanner(P＜0 .05) ,and the expression level of α-Smwaup-regulated with the increase of the glucose concentration in dose-dependenmanner(P＜0 .05);the albumin inflow amounof podocytewaup-regulated with the in-crease of the glucose concentration in dose-dependenmanner(P＜0 .05) .The expression amounof GSK-3βin the high glucose group waincreased(P＜0 .05) .Compared with the control group ,the expression amounof nephrin and podocin aftehigh glucose treatmenin the GSK-3β siRNtreatmengroup waincreased ,while the expression of α-Smwadecreased .Conclusion The high glucose condition could induce the phenotypichange and functional impairmenof mice podocyte;GSK-3βparticipatein the epithelial-mesenchymal transdifferentiation procesof podocyte undethe high glucose condition .

Objective To observe the therapeutic effect of phloroglucinol combined with diazepam in prolonged or protracted active phase of first stage of labor,and the outcome of perinatal phase.Methods Two hundred vaginal delivery primipara with prolonged or protracted active phase after dilatation of cervix 2-3 cm were selected.The patients were divided into 2 groups by random digits table method with 100 cases each,the patients in observation group were given phloroglucinol 80 mg and diazepam 10 mg,and the patients in control group were given diazepam 10 mg.The cervical dilatation,the degree soft hardness and edema of cervix,time of active phase,time of second stage of labor,mode of delivery,vaginal bleeding in 2 h postpartum and outcome of perinatal phase in the 2 groups were observed.Results In observation group,the cervical dilatation rate was (2.31 ± 0.27) cm/h,the time of active phase was (187.27 ± 33.22) min.In control group,the cervical dilatation rate was (1.82 ±0.48) cm/h,the time of active phase was (251.32 ± 45.33) min,there were statistical differences between 2 groups (P ＜ 0.05).The rate of uterineincision delivery in observation group was significantly lower than that in control group [18.0％(18/100) vs.32.0％ (32/100)],there was statistical difference (P ＜ 0.05).There were no statistical differences in time of second stage of labor,vaginal bleeding in 2 h postpartum,rate of neonatal asphyxia between 2 groups (P ＞0.05).Conclusion The effect of phloroglucinol combined with diazepam in prolonged or protracted active phase is remarkable,it can promote the progress of labor,speed up delivery progress,improve the rate of vaginal delivery and decrease the rate of uterine-incision delivery,and no adverse effects on the outcome of perinatal phase,it is worth popularizing.

Objective To explore the risk factors of breast cancer recurrence for the cause exploration and prevention of the disease.Methods With matched case-control method,a survey was conducted among survival cases of histologically diagnosed breast cancer patients in Southern hospital from January 2004 to December 2007 in Guangzhou city.The survival cases were divided into case group and control group according to the inclusion criteria.Unconditional logistic model was used to analyze the data.Results Multiple factors logistic regression analysis showed mental stimulations [OR =10.926,95％CI (5.041-23.682)],oral contraceptive pills history [OR=2.634,95％CI(1.548-4.482)],benign breast disease history [OR=5.183,95％CI(2.100-12.790)],human epiderma growth factor receptor-2 (HER2) positive [OR=2.257,95％CI (1.326-3.843)],lymph node metastasis [OR=2.021,95％CI (1.272-3.212)],distant metastasis [OR=3.718,95％CI (2.125-6.503)] might be associated with the increasing breast cancer recurrence risk.Regular exercise [OR=0.460,95％CI(0.234-0.905)],low stage of disease [OR=0.106,95％CI(0.022-0.498)],breast nursing time [OR=0.053,95％CI(0.009-0.316)],dietary intake of soy isflavones[OR=0.154,95％CI (0.032-0.745)] might be associated with the decreasing breast cancer recurrence risk.Conclusions Clinical medical staff should take attention to the breast cancer who had benign breast disease history to ensure timely check-ups,early detection,early diagnosis and early treatment.Improving physical and psychological quality,reducing oral contraceptive pills,prolonging breast nursing time,and increasing dietary intake of soy isflavones were important to the prevention of breast cancer recurrence.

Objective To evaluate the clinical effects of magnetic attachments in complete overdenture. Methods Casting method was used to fabricate Coping-Keepers for remained roots, and the patients were followed up for 6 months to check the effects of the overdentures retained with Magfit EX attachments. In the experimental group, 21 complete overdentures were made with 33 Magfit EX magnetic attachments for 18 patients. Eleven patients treated with traditional complete overdentures were selected to act as control. The retention force, aspect and comfortableness were subjectively evaluated by the patients of the both groups. Abutment status was observed and compared before and after cementation of the magnetic attachments by measuring periodontal index, including gingival index (GI), sulcus bleeding index (SBI) and bleeding on probing (BOP), etc. Masticatory function of patients in the both groups was measured at 3 time points: immediate, 3 months and 6 months after cementation. Results Patients in experimental group were more satisfied with complete overdentures made up of magnetic attachments than those in control group, especially in retention force and masticatory efficiency and aspect. Magnetic attachments can provide enough retention force for patients and greatly improved the masticatory efficiency and the abutment condition. The masticatory function was to be perfect and stable after 3-6 months. Furthermore, the mobility and periodontal index of most abutments improved after 6 months. Conclusion It is proved that magnetic attachments are effective to improve the function of complete overdentures, worthy to be used widely. It is suggested that the residual root and loosened tooth should be preserved furthest as possible in clinic.

The morbidity of young non-small cell lung cancer(NSCLC)increases in the recent years.Young group patients should be defined as age under 40 years old.The risk factors of etiology in the young NSCLC may be related with smoking,genetic predisposition,Internal and external environment factors havoc and etc.There is no specific symptoms of young NSCLC,therefor,it happens misdiagnosis and harder to be confirmed.The morbidity in young female group of NSCLC patients is higher than aged patients.The most frequent histopathologic type is adenocarcinoma and the ratio of advanced stages with low differentiation has been found to be more common.The chances of surgery seems lower than others.EGFR gene mutation percentage in the young NSCLC maybe lower than that in the old patients,while the positive mutation of MEL4-ALK gene maybe more prevalent in the young patients.The prognosis of young NSCLC seems to be poor with lower tumor progression time of the first line therapy.Better prognosis of young NSCLC patients maybe achieved by early diagnosis and efficient therapy according to the accurate genotypes.