Objective To study the changes in needs of standard training for general practice during the period of 2000 to 2006.Methods Sixty-two trainees participated in a municipal training program for general practitioners(GPs)in Zhongshan Hospital,Shanghai were interviewed by questionnaire to understand their recognition of general practice and needs in training for general practice,and compared to other 53 trainees who entered an another training program in 2000.Results Nineteen of 53 trainees(36percent)trained in 2000 could understand all the six aspects of general practice,including medical and health care,preventive care,rehabilitative care,health education and family planning techniques service,51(94 percent)were willing to accept such training,and 30(57 percent)did not satisfy with current conditions in community health care service.As compared,their recognition for general practice,acceptance of training,and satisfaction with their working condition improved obviously in trainees trained in 2006,with proportions of 100 percent,100 percent and 98 percent,respectively.But,main reason for dissatisfaction with community health care service could still attribute to less attention paid to general practice by relevant leadership.Conclusions Nowadays.the needs in general practice training by health care workers in communities increased greatly,but which still should be supported by governmental policies.

Objective To design a novel strain loading device for studying the mechanical biology of adherent cells. Methods Based on the technology of substrate deformation loading, the device adopted controllable stepper to cause deformation of the silastic chamber, so as to realize cell loading with multiple units and large strain. The device was developed to test its loading functions. The three-dimensional (3D) models of the silastic chamber were established to simulate the loaded chamber by the finite element technology, and uniformity of the strain field was analyzed. The device applied 5% strain to bone marrow stromal cells (BMSCs) with 0.5 Hz stretch frequency at 2 hours per day for 5 days, and an inverted phase contrast microscope was used to observe the morphology of BMSCs. Results The developed strain loading device for adherent cells in vitro could provide mechanical unidirectional strain up to 50% with three groups of cell loading substrates; within the 10% stain range, the area of uniform strain filed on the silastic chamber remained above 50%, which ensured that the cells were loaded evenly; the morphology of BMSCs was obviously altered, and the direction of arrangement tended to be perpendicular to the loading direction of principal strain. Conclusions The device shows the advantages of reliable operation, wide strain range, adjustable frequency and convenient operation. It can be used to load multiple cell culture substrates at the same time, which provides convenient conditions for the study of cell mechanobiology.