Currently, the commonly used diagnostic markers have the shortcomings of lower sensitivity or specificity in the early diagnosis of cardio-cerebrovascular diseases. Therefore, it needs to use the novel specific biochemical markers for the early diagnosis arid treatment, so as to decrease the high mortality and disability caused by cardio-cerebrovascular events. Fatty acid-binding proteins (FABPs) are a family of low-molecular-weight intracellular lipid-binding proteins. They are divided into 9 different subtypes, including the liver-, intestinal-, heart-, brain-, adipocyte-, skin-, ileal-, myelin-, and testis-subtype. Their primary function is to transport long-chain fatty acids into the cells, and thus regulate intracellular lipid metabolism. The structures of all FABP subtypes are similar, inclusive of two α2 helices arid one β2 fold structure. Among them, the sensitivities and specificities of the heart- and brain-subtype FABPs are higher in the early diagnosis of cardio-cerebrovascular diseases. It is promising to become the novel markers in the early diagnosis of cardio-cerebrovascular diseases.

Nobel Prize 2009 in Physiology or Medicine is awarded to three American scientists, Elizabeth H.Blackburn, Carol W.Greider and Jack W.Szostak, for the discovery of"how the chromosomes are protected by telomeres and the enzyme telomerase".Telomere is the specific structure at the ends of the chromosomes and protects it from fusion and degradation.Telomerase synthesizes telomere DNA to maintain the telomere length.Studies suggest that telomere length and telomerase activity is directly associated with cell life and the genesis of many diseases.With the progress of study, how to control the telomere length and telomerase activity is helpful to shed light on the studies in"cancer, inherited diseases and senescence", and will stimulate the development of potential new therapies.

@@

3.00 and exposed to the solution containing 1500 mg/L peracetic acid for 30 min could completely killing the spores.Aerosol spraying with solution containing 1000 mg/L peracetic acid at a dose of 10 ml/m3 for 30 min caused over 90.0% decay rate of natural bacteria in indoor air.CONCLUSIONS Weikangshi peracetic acid has good stability and its germicidal efficacy is not affected.

Objective To study the effctiveness and safety of arsenic trioxide(As2O3)combined with Homoharringtoninum in the chronic myelocytic leukmia-chonic phase(CML-CP).Methods Seven patients were treated with As2O3 10mg per day for 2~3 week and with Homoharringtoninum 3~4mg per day for 1~2.Results All patients were clinic remission,of which there were 4 complete remission who were all initial therapy,and 3 partial remission,2 of who were initial therapy and 1 was resume threapy.4 patients of CR were treated with second strengthen therapy and continued hematologic CR.The main side effects were grade 3 hematologic toxicity and light liver damage,and no significant nausea,emesis,diarrhea or mucositis.Conclusion As2O3 combined with Homoharringtonium in the CML-CP is a safe and effctive regimen.

Objective To observe the protective effects of le g negative pressure (NP) treatment on free radical injury from chronic ischemia of lower legs in canines. Methods By cutting off the branches of the femoral artery a nd inserting two pieces of spiral wires into the artery of canine posterior limb , we succeeded in duplicating the animal model of arterial occlusive disease in the lower extremity within two weeks. After treatment with LNP for 8 to 10 days, the blood samples of the ischemic legs were collected and the lipid peroxide (L PO) content as well as superoxide dismutase (SOD) activity were measured and ana lyzed, respectively. Results When the ischemic model was set up, PLO content inc reased while SOD activity decreased apparently, compared with those of normal [(35.594?10.753)vs. (24.800?9.011)?mol?L -1, P

BACKGROUND:intervertebral disc degeneration can reduce nucleus pulposus cells,and peroxiredoxin II involved in the regulation of resist oxidation damage,cell division,differentiation,signal transduction and apoptosis.Peroxiredoxin Ⅱ has promotive effect on intervertebral disc degeneration,whereas the mechanism remains poorly understood.OBJECTIVE:To observe the effects of perexiredoxin Ⅱ on human intevertebral disc cells activity and type Ⅱ collagen synthesis in vitro.METHODS:Human degenerated human lumbar disc cells were cultured in vitro,and assigned into the control and peroxidase Ⅱ groups.Peroxidase Ⅱ with doses of 10,100 and 1 000 ng/L were added into the peroxidase Ⅱ groups.The cells were identified by immunohistochemical staining,and the cell proliferation was detected using cck-8 kit.Cell supematant was collected at days 3 and 7 after operation,and the expression of type Ⅱ collagen was measured by double-antibody sandwich enzyme-linked immunosorbent assay.RESULTS AND CONCLUSION:In vitro cultured human degenerative lumbar intervertebral disc nucleus pulposus cells by adding peroxidase increased with the dose-Ⅱ,the disc nucleus pulposus cells of the volume and type Ⅱ collagen synthesis gradually reduced(P ＜ 0.01).Tips peroxidase Ⅱ on the intervertebral disc nucleus pulposus cells,the number and type Ⅱ collagen synthesis significantly inhibited in a dose-dependent manner.Thus speculated that peroxidase Ⅱ on the nucleus pulposus cells in vitro may lead to disc degeneration as a precipitating factor.

BACKGROUND:The hemodynamics changes of aneurysm provide the basis for aneurysm treatment and ruptured aneurysm prevention, while finite element analysis is a good technical means.
OBJECTIVE:To construct the two-way flow-solid coupling model of internal carotid artery aneurysm, and thus simulate its flows.
METHODS:CT angiography data of internal carotid artery aneurysm were recorded with GE Lightspeed 64 spiral CT scanning, and the corresponding three-dimension model was constructed with mimics10.01 software. Then the fluid-solid coupled flow simulations were done depending on Ansys+Fluent software.
RESULTS AND CONCLUSION:The two-way flow-solid coupling model of internal carotid artery aneurysm was built, with the same morphology and anatomy as the three-dimensional CT imaging. In the whole cardiac cycle, the blood flow of aneurysm body was swirl typed and its velocity was slower than that of the aneurysm neck;the deformation, wal shear stress, pressure and von Mises stress of aneurysm wal were the maximum at the neck, and the minimum at the top. Their highest value was at the 0.16 s (rapid ejection period), and their lowest value at the 0.74 s (relaxation period). On the basis of CT angiography data, to construct the two-way flow-solid coupling model of internal carotid artery aneurysm is a simple and practical method. The blood flow simulation of aneurysm is close to the human physiological conditions, and the results wil provide new theoretical basis for the study on the occurrence and development of aneurysm.

Objective To study the diagnosis, treatment, etiology and prognosis of scrotum gangrene. Method We did a retrospective analysis of the data of 78 patients with scrotum gangrene who hospitalized during August 2007 and August 2011 in Jinja Hospital in Uganda.Results Among 78 patients, 73 patients were cured, with 93.6%of the cure rate. After early debridement and second phase reconstruction, patients recovered after the treatment. Conclusion On the basis of systemic antibiotic drug use and local debridement processing, penis-scrotum revascularization has good curative effect on scrotum gangrene.We hope to provide reference and help to foreign health medical team of aid work in Yunnan province.

Objective To investigate the longitudinal systolic ventricular function of children with single ventricle, and to appraise the feasibility and clinical value of velocity vector imaging (VVI) on assessing it. Methods The study group consisted of 30 patients with functional single ventricle. The control group consisted of 30 age- and gender-matched normal children. Using Sequoia C512 echocardiography machine,the peak velocity(V),peak displacement(D) ,strain(S) ,and strain rate(SR) were measured. Results Basel and median velocities,as well as basel and median displacements of rudimentary chamber side(RCS), were lower than those of septum[Basel velocity (1. 93 ± 0. 71)cm/s vs (3. 53 ± 1. 07)cm/s;Median velocity (1.19±0. 57)cm/s vs (2. 03 ± 0. 90) cm/s; Basel displacement (2. 53 ± 1.65) mm vs (6.21 ± 2. 12)mm;Median displacement(1. 26 ± 1. 06)mm vs (3. 21 ± 1.37)mm]. The values of strain of all the six segments of single ventricle were significantly lower than Corresponding segments of the control group [ basel nonrudimentary chamber side(NRCS) ( - 16. 17 ± 4. 37) % vs ( - 19. 66 ± 3. 47) % ; Median NRCS ( - 15. 23 +4.36)% vs (-19.64± 4. 75)%; Apical NRCS (-13.84 ± 5.79)% vs ( - 16. 7 ± 4. 15)%; Basel RCS(-10. 54±5.35)% vs (-19.49±3. 74)%;Median RCS ( - 10. 16 ± 5. 26)% vs ( -20. 83 ± 3. 82)% ;Apical RCS ( - 10. 97 ± 5. 22) % vs C - 18. 11 ± 4.43) %]. Aside from basel strain rates of NRCS, strain rates of all the other five segments of single ventricle were significantly lower than Corresponding segments of the control group [Median NRCS ( - 1. 21 ± 0. 42)s-1 vs (- 1.49 ± 0. 24)s-1 ;Apical NRCS ( - 1.10 ±0.41)s-1 vs (-1.47 ± 0.24)s-1;Basel RCS ( - 1.07 ± 0. 35) s-1 vs (- 1. 49 ± 0. 22) s-1; Median RCS (-0.97± 0.34)s-1 vs ( -1.48 ± 0. 20)s-1 (Apical RCS ( - 0. 93 ± 0. 39)s-1 vs ( - 1. 48 ± 0. 22)s-1]. Conclusions Longitudinal systolic ventricular function is impaired in children with functionally single ventricle. VVI can be used to asses ventricular systolic performance in children with functionally single ventricular.