Health history,clinical syndrome and imaging examination are key factors for early diagnosis of pancreatic trauma,while the effects of the test of the serum amylase for the diagnosis of pancreatic trauma remain unclear.Laparotomy plays an important role in the diagnosis of pancreatic trauma for patients,particularly urgent patients.The grading of pancreatic trauma functions as a bridge between the diagnosis and treatment of the pancreatic trauma.So far,the organ injury scale grading system proposed by the American Association for the Surgery of Trauma (AAST-OIS) provides guidelines for operative versus nonoperative management in solid organ injuries.Surgery is an important treatment method for pancreatic trauma according to injury control and surgical procedures grading principles.Recently,minimally invasive surgery has been involved in the diagnosis and treatment of the pancreatic trauma.

As a result of the early amplification of the inflammatory response in the acute pancreatitis, systemic inflammatory response syndrome (SIRS) is a main cause of acute pancreatitis-associated lung injury (APALI) , while early combined acute lung injury or acute respiratory distress syndrome causes a high mortality of acute pancreatitis.A series of inflammatory mediators and cytokines play important roles in the process of SIRS and APALI, therefore, inflammatory reaction restoring a balance becomes a key point of the treatment of pancreatitis lung injury.

With the enhancing treatment concept and the improvement of diagnosis and treatment technology in recent years,multidisciplinary cooperation and minimally invasive treatment has been the dominant treatment methods for acute pancreatitis,and they reduces the mortality effectively.At the same time,a series of new treatment methods such as step-up approach,which has obvious advantages over traditional surgery,improves the prognosis of severe acute pancreatitis significantly,and it has been recognized by scholars both at home and abroad.

Objective To analyze the influence of multiple myeloma tumor-associated gene MYEOV2 on NIH/3T3 cells growth. Methods The recombinant plasmid, pcDNA3.1(+)/MYEOV2, was constructed and subsequently transfected into NIH/3T3 cells by liposome. The positive clones of the transfected NIH/3T3 cells were obtained by G418 selection. RT-PCR was used to detecte the expression of MYEOV2 gene in the cells. Fluorescence-activated cell sorting (FACS) and cell grow curve drawing were applied to analyze the influence of MYEOV2 gene on NIH/3T3 cells proliferation. Results The cell clones stably expressing MYEOV2 gene were obtained. FACS analysis showed that the percentage of S phase cell of pcDNA3.1(+)/MYEOV2-transfected cells, pcDNA3.1(+) -transfected cells and non-transfected cells was 30.9%, 20.1% and 16.1%, respectively, and the difference of sphase cell percentage between pcDNA3.1(+)/MYEOV2-transfected cells and the other two groups of cells was significant (P

Objective To explore the effects of music games thematic curricula on preschool children with intellectual disabilities. Methods 30 children with intellectual disabilities accepted music games thematic curricula (experiment group, n=15) or routine thematic curricula (control group, n=15) respectively. They were assessed with the Scales of Learning Attitude, Scales of Emotive Behavior, Scales of Communicational Ability, and Scales of Social Skill before and after 20 weeks of curricula. Results The experiment group performed better in learning attitude, emotive behavior, communicational ability, and social skill than the control group (P<0.001). Conclusion Using music games in thematic curricula can not only improve the mood and behavior of the preschool children with intellectual disabilities apparently, but also inspire their learning interest, develop their communicational and social abilities.

Immobilization osteoporosis (IOP)is a common complication in clinic and cosmonauts. It severely impaired the patients ant the cosmonauts. The causes of IOP are weightless,immoblization and bed rest.Excercise therapy is very important for IOP. The etiology andpathogenesis of IOP are introduced in this article. the effects, mechanism and methods of the exercise therapy for preventing and treating IOP arealso discussed.

Objective To summarize the clinical effects of cholecystocholangiostomy (STHG) in the treatment of hepatocholangiolithiasis and hepatic portal bile duct stenosis. Methods The early complications, clinical effects, and functions of gallbladder in a total of 135 patients, admitted since 1994 were for STHG were retrospectively analyzed. Results No early complications were observed after the operation. The contraction and concenration function of the gallblader remained basically normal. The color of gallbladder mucosa was normal,and no edema or erosion was found under choledochoscopy. Conclusions This operation not only maintained the normal physiological functions of the gallbladder and Oddi sphincter, but also the physiological flow of the bile, farthermere it prevented reflux of intestinal juice and reflax chdangitis. Because the anastomotic stoma was large, concentrated bile within the gallbladder could freely get access to the intrahepatic biliary tract, the concentration of bile acid in the intrahepatic bile duct was markedly elevated, so that the solubility of bilirubin crystal, was increased and recurrence of calculus formation eould be retarded. There fore, STHG should be considered to be an optimal operation in dealing with hepatocholangiolithiasis and biliary stenosis.

Objective To investigate the possibility of differentiation of multipotent adult progenitor cells from human bone marrow(hMAPCs) into hepatocytes with hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) in vitro to find a new cell source for liver tissue engineering. Methods (1) Harvest of the hMAPCs: The bone marrow mononuclear cells were obtained from bone marrow obtained from volunteers by gradient centrifuging. The adherent mononuclear cells were cultured. The mesenchymal stem cells(MSCs) were isolated and collected by magnetic activated cell sorting(MACS) through depletion selection by the use of CD45 and GlyA microbeads. (2) The hMAPCs were induced to differentiate with HGF(20ng/ml) +FGF-4(10ng/ml). L-02 human hepatocytes (cell lines) were used as the control, with undifferentiated hMAPCs serving as the contrast. (3)The expression of albumin (ALB), alpha fetoprotein (AFP), cytokeratin-18(CK-18), cytokeratin-19(CK-19) was detected with immunocytochemistry to identify the characters of the differentiated cells at different time, and to determine the ratio of positive cells. (4) ALB expression of the differentiated cells at different time was detected by Western blot on day 21 and 25. Results (1) The result of immunocytochemistry: The differentiated hMAPCs expressed the late markers (ALB, CK-18) of hepatocyte at all the time but did not express CK-19. The differentiated hMAPCs expressed AFP on day 7. (2) The result of Western blot assay: the differentiated cells expressed the late markers (ALB) of hepatocyte on day 21 and 35. Conclusions hMAPCs can differentiate into hepatocyte-like cells under specific inducing conditions.

Objective To discuss the accuracy of 3DSS three-dimensional scanning system, which was used to reconstruct the 3D digital model of unilateral orbital defect patients. Methods By using 3DSS spectrometer on the case of patients with unilateral orbital defect, the related scan dates of facial structure were collected, and the reconstructed digital models were processed wax pattern by SLS prototyping machine. Results The digital solid model of three-dimensional reconstruction was smooth and complete, and border over the natural. The designed resin prosthesis can be inosculated with the orbital defect tightly and symmetrically. Conclusion To adopt the system can be successfully completed unilateral orbital defect restoration design and production, and ultimately satisfied the clinical requirement of prosthesis production.

2.0 ?g/L,n=65 cases;Group B(low level):CRP