OBJECTIVE:To prepare compound rifampicin auristilla and to establish a method for its quality control.METHODS:The content of rifampicin was determined by HPLC and the sample was stored under the temperature of(6?2)℃for6months.RESULTS:The linear range for rifampicin was6～60?g/ml,the average recovery was100.33%(RSD=1.23%).The appearance,pH value and contents of the preparation were in conformity with the requirements after storage for6months.CONCLUSION:This preparation method of compound rifampicin auristilla is simple and cheap,and its quality is controllable.

Objective: To evaluate the satisfaction degree of non-medical students in medical school,and to investigate the factors that influence satisfaction degree. Method: Selfcompile scale is administered to 430 nonmedical students from 6 medical colleges,in which 5 is in Guangdong province and 1 is in Ha’erbin city. Result:The satisfaction of nonmedical students is low,significant diferences are found between grades,and between students with medicine as the first choice major and those whose first choice major is not medicine. Conclusion:Medical colleges should pay more attention to nonmedical majors,improve the quality of teaching,and so on in order to improve the nonmedical students’satisfaction degree.

Angiotensin converting enzyme 2 (ACE2) is a new member of renin-angiotensin system found recently. It counterbalances the effects of angiotensin converting enzyme (ACE), hydrolyzes angiotensin Ⅱ to generate angiotensin-(1-7) and hydrolyzes angiotensin Ⅰ to produce angiotensin-(1-9). Angiotensin-(1-9) can be hydrolyzed by ACE to form angiotensin-(1-7), and angiotensin-(1-7) counteracts the effects of angiotensin Ⅱ in many aspects. Bradykinin or atrial natriuretic factor or substance P are not the substrates of ACE2, and ACE2 hydrolyzes the metabolites of bradykinin such as des-Arg~9-BK and des-Arg~(10)-kallidin, and also the dynorphin A, one of the opium peptides. ACE2 is not inhibited by angiotensin-converting enzyme inhibitor (ACEI). Recent studies demonstrate that ACE2 is a functional receptor of SARS coronavirus. To elucidate the mechanisms of cardiovascular diseases and other disease such as SARS, further investigations in ACE2 are needed.

Objective The purpose of the present study was to explore the use of psychological intervention in modified electroconvulsive therapy patients.Methods Divide all 160 patients random into two groups,the intervened group and the contrast group. The intervened group was accepted psychological intervention before the therapy day;The contrast group just did as normal.Record the anxiety, blood pressure,heart rate and the agreement to the therapy of the two group before the therapy.Results The anxiety of the intervened group was much little to the contrast group.The blood pressure and the heart rate of the intervened group was lower than the contrast group.Patient of the intervened group was much active to match with the treatment. Conclusions The use of psychological intervention in modified electroconvulsive therapy patients could decrease patient’s anxiety and painness,made them more active to match with the treatment.

Objective To observe the protective effects of the mixture of ferulic acid (AF) and paeoniflorin on PC12 cells injury by FeSO4/H2O2 in vitro, and explorer its mechanism. Methods The cell injury model was established with FeSO4/H2O2 for 24 h. The process of the growth and morphology of the cells were observed under phase contrast microscope in vitro. The survival rate of the cells was determined by MTT method. The content of MDA and SOD activity was measured respectively by thiobarbituric acid and xanthine oxidase method. Results Compared with FeSO4/H2O2, the mixture of ferulic acid (AF) and paeoniflorin enhanced the PC12 cells survival rate and viability, reduced MDA content and increased SOD activity. Conclusion The mixture of 5～50 ?mol/L AF and 1～10 ?mol/L paeoniflorin exerted protective effects on PC12 cells injury induced by FeSO4/H2O2, which maybe related to free radical scavenging activity and lipid peroxidation process being inhibited.

Objective To study the incidence of hypertension in patients with different severity of obstructive sleep apnea hypopnea syndrome(OSAHS) and to observe the effects of short-term continuous positive airway pressure (CPAP) treatment on hypertension in patients with hypertension Ⅲ complicated with OSAHS.Methods According to sleep apnea hypopnea index (AHI) and the lowest oxygen saturation value at night,184 patients with OSAHS were divided into mild group(n =55),moderate group(n =63) and severe group(n =66).We observed the incidence of hypertension among patients and their mean systolic and diastolic blood pressures before sleep and after wakening.There were 46 patients with severe OSAHS combined with hypertension Ⅲ received short-term (at least 7 days) CPAP treatment.We observed and analyzed the effect of CPAP on blood pressure values.Results In the 184 cases,incidence of hypertension of the three groups were 36.4％ (20/55),42.9％ (27/63) and 62.1％ (41/66) respectively.The incidence of hypertension in OSAHS patients was significantly higher in the severe group than in the moderate group (x2 =4.799,P ＜ 0.05) and the mild group(x2 =7.962,P ＜ 0.05).There were significant differences among mild,moderate and severe OSAHS patients in mean systolic and diastolic blood pressures before sleep Systolic ((135.08 ± 6.71) mm Hg vs.(136.59 ± 15.14) mm Hg vs.(144.73 ± 15.80)mm Hg,F =4.202,P ＜0.05) and Diastolic((90.96 ±6.06)mm Hg vs.(91.41 ± 4.05) mm Hg vs.(94.13 ± 13.97) mm Hg,F =13.690,P ＜ 0.05)) and after wakening Systolic((136.83 ± 8.23) mm Hg vs.(137.89 ± 17.03)mm Hg vs.(148.59 ± 14.83) mm Hg,F =4.158,P ＜0.05) and Diastolic((91.25 ± 9.96) mm Hg vs.(93.40 ± 8.82) mm Hg vs.(98.75 ± 9.39) mm Hg,F =5.638,P ＜0.05)).Severe OSAHS patients had significantly higher systolic and diastolic blood pressures after wakening than before sleeping (P ＜ 0.05).The changes of blood pressure had significant difference after CPAP treatment than before CPAP treatment (Systolic:(175.09 ± 9.66) mm Hg vs.(168.22 ± 8.55)mm Hg,t =3.556,P＜0.05;Diastolic:(105.82 ± 12.44) mm Hg vs.(100.61 ± 11.26) mm Hg,t =2.741,P ＜0.05).Conclusion OSAHS and hypertension are closely related to each other.CPAP is an effective treatment to OSAHS combined with hypertension.

Objective To investigate effects of combined use of glycogen synthase kinase (GSK) -3 inhibitor and fructose-1,6-diphosphate (FDP) on liver trauma in rats. Methods After crea-tion of liver trauma model in 49 Sprague-Dawley rats, 42 rats were randomly divided into control group (NaCl group), FDP group and FGI Group (FDP and GSK-3 inhibitor in combination group). Then, each group was randomly subdivided into pre-ischemia group and 4-hour reperfusion group on account of time point when animals were sacrificed before and after iachemia. The other seven rats set as sham operation group (SH group) were sacrificed at 4-hour reperfusion time point. The AST and ALT levels in hlood and glycogen content, SOD vitality and MDA content in liver tissues were determined. Results At pre-is-chemia time point, liver glycogen content in three groups was in order of control group < FDP group < FGI group (P <0.01). At 4-hour reperfusion time point, blood ALT and AST levels in four groups were in order of control group > FDP group > FGI group > SH group (P < 0.01), while SOD vitality in liver tissues of four groups was in order of control group < FDP group < FGI group < SH group (P < 0.01) and MDA content in four groups was in order of control group > FDP group > FGI group > SH group (P < 0.01). Conclusions Combined use of FDP and GSK-3 inhibitor can enhance the protective effect of FDP on liver rupture, as may relate to the mechanism that GSK-3 inhibitor can effectively enhance glycogen synthesis of FDP as substrate before liver ischemia so that the liver glycogen storage is increased in a short period of time and hence post-traumatic warm ischemia-reperfusion injury is alleviated in the liver of rats.

Objective To explore the relationship of gallbladder emptying function and its hemodynamics in diabetic patients. Methods Gallbladder volume ,emptying function and its arterial blood flow were measured by ultrasound. Results The gallbladder volumes of fasting (FV ) and postprandial (RV) increased and the ejection fraction (EF) of gallbladder was decreased in DM group than in NC group (P<0.01). In DM group ,the gallbladder artery’s resistance index (RI) was higher than in NC group [(0.61 ± 0.06) vs (0.70 ± 0.08)](P<0.01). Minimum diastolic blood flow velocity (Vmin) was lower than in NC group [(8.72 ± 1.43) vs (6.05 ± 1.89)cm/s](P<0.01). RI was associated with HbA1c (r= 0.827 ,P< 0.01) and LDL‐C(r= 0.820 ,P< 0.01). Conclusion Gallbladder emptying dysfunction in diabetic patients is associated with the hemodynamics of gallbladder.

In this study we successfully prepared desmoglein 1 and desmoglein 3 DNA probes by incorporation of Dig-dUTP during PCR amplification. Eleetrophoresis analysis showed that Dig labelled probes moved slower on the gel than PCR products not incorporated with Dig due to the enlargement of probe fragment. The results of Dot-blot showed that the prepared probes can be used for RNA analysis, and both probes have high specificity, The results provided basis for the quantitative and qualitative study of desmoglein gene expression in tissues or cells by means of in situ hybridization and Northern hybridization etc.

Objective In order to provide scientific evidence for revealing the pathophysiological changes, levels of the humoral and cellular immunity and cytokines in elderly patients with bacterial pneumonia were determined. Methods The changes of immunological markers including IgA, IgG, IgM,C3, C4,T cell subpopulation (CD3 +, CD4 +, CD8 +) and CD4 +/CD8 +, IL 2, IL 6, IL 8, TNF ? were examined for the elderly bacterial pneumonia group(BPG), the aged control group(ACG) and the young control group(YCG). Results The level of CD3 +〔(53 90?7 10)% vs (62 67?6 08)%〕, CD4 +〔(33 85?4 69)% vs (3 57?3 40)%〕, IL 2〔(4 09?0 69) ?g/L vs (6 72?0 52) ?g/L〕 and IL 8〔(0 28?0 07) ?g/L vs (0 39?0 09) ?g/L〕 in ACG were significantly lower than those in YCG. The levels of IgA, IgG, IgM, C3, CD8 +, IL 8〔(0 33?0 08) ?g/L vs (0 28?0 07) ?g/L〕, and TNF ?〔(1 25?0 23) ?g/L vs (1 10?0 20) ?g/L〕in BPG were significantly higher than those in ACG; and in BPG the levels of IgG, IgM, C3, IL 8 and TNF ? were significantly higher than those in YCG. However, the levels of CD3 +, CD4 + and the ratio CD4 +/CD8 +in BPG were markedly lower than those in YCG;the IL 2(2 730?0 612 ?g/L) was much lower than that of ACG〔(6 72?0 52) ?g/L〕 and YCG〔(4 09?0 69) ?g/L〕, respectively. Conclusions The occurrence and the progress of bacterial pneumonia in the elderly are related to the immunological functioning including the excessive humoral immunity, the super activation of complement system, the dysequilibrium of T cellular immunoloregulation network and the super excretion of inflammatory cytokines.