Hightiter anti-idiotypic antibodies(Ab_2)sera and two monoclonal anti-idiotypic antibodies (McAb_2)were repared by immunizating syngeneic BALB/c mice wth a complex of 7A_4 monoclonalantibody(McAb_1)against the a eterminant of the Hepatitis B Surface antiger(HBsAg)。Both Ab_2sera and McAb_2 recognize the idiotype of aratope n 7A_4McAb_1.Ab_2 sera contain the antibodiesbinding specially to anti-HBs from other species(guinea pig、onkey、rabbit and goat)and the twoMcAb_2 can not bind to these anti-HBs.Therefore it is thought that the two McAb_ re noninternalimage(Ab_2?)anti-idiotypic antibodies,this study proves that the McAb_1 against he eterminantof the HBsAg are different in their paratope,The McAb_2 can be used to stinguish the parotope onthe McAb_1 as a biological probe.

Network theory is applied to the optimization study on field medical equipment allocation with patient flow and its rate in wartime analyzed. With the application of queuing theory and linear programming, different optimized combinations of evacuation tools are studied, and then static model and dynamic model are established. A calculating table on queue length, service intensity and service desk is put forward to realize rapid evaluation of quantities of required field medical equipment for PLA. Some suggestions are provided to modify the current standard of PLA's basic medical equipment.

Objective To find out the factors which influence the establishment of collateral circulation as well as its clinical characteristics by analyzing the sufferers' medical records.Methods Review and collect the records of the first coronary angiography,and we can find the total occlusion of one coronary after coronary angiography.There are 52 cases in which people who suffered the establishment of collateral circulation stay in hospital for treatment.Here,we take 52 cases in which people didn't suffer into comparison and analyze these clinical records.Results Use the Logistic regression method to analyze the relationship between general clinical data,past history,habits,current medication and the establishment of collateral circulation,which relates to the diabetes mellitus anamnesis,exercise and drinking habits,with the regression coefficient is 0.408,0.483,0.197(P

Humanistic quality of medical workers directly affects the quality of medical service and patient satisfaction.In view of patient′s value demand,this paper identified main setbacks in humanistic service in China in the course of medical service.The authors found out the reasons for the lack of humanities quality,and interpreted medical humanistic spirit,medical professional spirit,medical ethics and morality,and connotation of medical law culture.On such basis,the paper unveiled the importance of medical humanities accomplishment in medical practice and medical students′training,proposing to promote the concept of humanistic spirit of medicine and the doctor-patient communication skills.

Percutaneous mechanical circulatory support (PMCS) devices are effective in the treatment of patients with cardiogenic shock and acute decompensated heart failure, as a bridge to the recovery of heart function or further treatment. The intra-aortic balloon pump (IABP) is now the most widely used PMCS. New PMCS devices including Transseptal device of Tandem-Heart, transaortic valve device of Impella and extracorporeal membrane oxygenation (ECMO) might provide more effective hemodynamic supports. Doctors should choose appropriate PMCS devices and their working modes, according to patient's clinical conditions, based on the working principles and hemodynamic effects of the devices, in order to achieve the best effects and help patients live through the crisis of diseases.

Objective: To compare the phenotype of induced dendritic cell (DC) derived from peripheral blood of the healthy individuals and the patients with oral carcinoma. Methods:15 patients with oral carcinomal at stages II or above were assigned to study group and 15 cases of healthy volunteers were assigned to control group. Leukocyte were isolated by density centrifugation. Adherent fractions were incubated in present of GM-CSF,IL-4 and TNF-? for 7 days in vitro. The morphological change of the cultured cells was observed by light microscopy and electronic microscopy. The phenotype expressions of CD83,CD1a,CD86, CD80, HLA-DR were analysized by flow cytometry. Results:Typical DCs were obtained from two groups after 7 days culture. The expression rate of CD83,CD1a on DC from healthy volunteers was significantly higher than that from patients (P0.05). Conclusion:DC derived from oral cancer patient' s peripheral blood monocyte might be more matured and is a good cell model for further immunotherapy and research.

50% resolution of elevated ST-segment in 1h after PCI was increased in the L-CN treated group. LVEDD was less but LVEF was higher in the treatment group compared with the control group 3 months after PCI. Conclusion L-CN has significant myocardial protection effects in patients with AMI undergoing primary PCI, which is characterized by inhibition of the reperfusion injury , reduction of infarction area and improvement of left ventricular remodeling.

Objective To investigate the effect of Honghua Injection on the activity of rat liver CYP2D6.Methods The metabolic rates of probe dextromethorphan(DM) in the blank and Honghua Injection groups(0.9,1.8,and 3.6 mL/kg) in vivo urine and in vitro liver microsome incubated system were determined by high performance liquid chromatography(HPLC).The variation of the metabolic rate of DM represented the effect of Honghua Injection on the activity of rat liver CYP2D6 in vivo and in vitro.Results In vivo and in vitro,the DM metabolic rates of treated groups(1.8 and 3.6 mL/kg) are lower than that in the blank group(P

Objective To explore the relationship of the number of balloon inflations during primary percutanoeus coronary intervention(PCI) with myocardial perfusion and the extent of myocardial necrosis,and to identify the potential clinical efficacy of ischemic postcondioning in patients undergoing Primary PCI.Methods One hundred and fourty patients with ST-segment elevation myocardial infarction(STEMI) who underwent primary PCI were eligibly enrolled for retrospective analysis.The infarct-related artery(IRA) in all patients had to be either left anterior descending artery(LAD) or right coronary artery(RCA) with TIMI 0 flow grade and no collateral filling prior to intervention,and had to be completely reperfused with TIMI Ⅲ flow grade after PCI.Patients with cardiac arrest,cardiogenic shock,previous MI and pre-infarct angina were excluded.Patients were divided into two groups for analysis based on the number of balloon inflations performed during primary PCI:Group A included all patients in whom 1-2 inflations were applied,while patients in Group B received 3 or more inflations.Myocardial perfusion was evaluated by myocardial blush grade and ST-segment resolution.The extent of myocardial necrosis was evaluated by peak values of CK and CK-MB release and predischarge left ventricular ejection fraction(LVEF) measured by echocardiogram.Results The 2 groups showed similar baseline characteristics,while patients in Group B received more balloon inflations than those in Group A(4.7?0.9 vs.1.8?0.2,P0.05),but decreased to 1.65?0.49 mm and 0.98?0.37 mm(P

Therapeutic alliance is one of the most popular topics in psychological counseling and psychotherapy research,but the concept of it is not unified.Despite of its various versions,most scholars regard it as an important relationship variable,emphasizing the agreement between therapist and patient as to the goals and tasks of treatment,and the affective bond established in the therapeutic dyad.Related research show that the characteristics of therapist and patient,and the interaction in this therapeutic dyad are the related factors on the formation and development of therapeutic alliance,which constitutes a major clinical variable in explaining and predicting the course and outcome of treatments.Future researches should further clarify the related factors of therapeutic alliance,expand the clinical applications of therapeutic alliance and strengthen the exploration of indigenization.