To discuss the relationship between Chinese medicine processing and medicine usage.After being processed,characteristics of Chinese medicine can be changed,the performance be mitigated,and some side effects be reduced or eliminated,which makes its clinical usage more rational and effective.besides,medicine processing can also change the taste and reduce bad smell of medicines.

The rapid advances over the past decades in molecular biology led to new ideas and methods to investigate the mechanism of depression, which enlarged the views of fields from neurological transmitters and related receptors to post-receptor signal transduction levels. It not only help to elucidate the pathogenesis of depression, but also offer beneficial evidence for development of new antidepressants. This paper reviews the development of molecular biological study in depression.

Objective To study and analyze the effect of fluoride faction shouted tons melitracen sheet (Deanxit)on abdominal pain symptoms of functional gastrointestinal disorders (FGIDs).Methods 82 patients with abdominal pain FGIDs were randomly divided into A group(n =40)and B group(n =42).A group was given conven-tional treatment,group B was given deanxit treatment on the basis of A group.The treatment efficacy and the total inci-dence of adverse reactions were analyzed.Results The total effective rate of group B (90.24%)was significantly higher than that in group A (65.85%),the difference was statistically significant (χ2 =4.538,P <0.05).There were no serious adverse reactions observed during treatment in the two groups,the incidence rate of adverse reactions between the two groups had no statistically significant difference (P >0.05).Conclusion Deanxit in the treatment of FGIDs can achieve satisfactory therapeutic effect,the drug can significantly alleviate the symptoms,andit is safe and without serious side effects.

Body weight, height, weight index as well as obesity incidence of 4780 people (male 3216, female 1564) aged from 20 to 78 including various kinds of job were investigated in Fusan district in Shandong province. The body weight and height were measured by conventional method. The weight indexwas estimated in terms of the value of weight(kg)/height(cm)2 ? 100. The correlationregression between sex, age, height and weight was calculated as well.The result showed that the average weight index ranging from 0.1933 to 0.2525 in males and 0.1951 to 0-2563 in famales was noticed. Accordingly, the incidence of overweight and obesity was 7.71% and 8.33% in males and 6.96% and 4.73% in females respectively. The correlation coefficients between weight and age (r = 0.9838 within 20-35 in age in males, r = 0.9846 within 20-50 in age in famales), and between weight and height (r = 0.9798 within 144-180cm in males, r = 0.8595 within 140-170cm in females) were highly significant.The data mentioned above were also discussed briefly.

It was shown that in the stricture on abdominal aorta (SAA) rat myocardiac hypertrophy model,the caudal blood pressure was increased,the wall of left ventricle was thichened, the proliferation of T cell in thymus and spleen was decreased and the content of IL-2 was less than normal. Acanthopanax giroldii polysaccharide (AGPS10mg/kg.d, sc) could harmonize the heightened blood pressure and increase the level of immunity in the body. It was suggested by the result, that AGPS could be used to strengthen the heart function as well as immunity.

Test on coagulation time,prothrombin time and platelet aggregation of mice and rats showed that all four species of Puhuang had effects on blood coagulation, in which Typha angustata Bory et Chaub. was the strongest followed by T. davidiana Hand. -Mazz., T. latifolia L,and T. angustifolia L. Their mechanism of coagulation were not the same,in which T. angustata Bory et Chaub. mainly acted on thrombogen,while T.davidiana Hand. -Mazz. mainly acted on platelet aggregation.

Objective To evaluate the changes in the expression of NRF-1 in the spinal cord during remifentail-induced hyperalgesia in a rat model of incisional pain.Methods Forty-eight Sprague-Dawley rats,weighing 180-220 g,were randomly divided into 4 groups (n =12 each) using a random number table:control group (C); incisional pain group (group Ⅰ); remifentanil group (group R); incisional pain + remifentanil group (group Ⅰ + R).All the rats were anesthetized with sevoflurane.A 1-cm longitudinal incision was made through skin,fascia and muscle of the plantar aspect of the right hindpaw in I and I + R groups.In C and I groups,normal saline was subcutaneously infused for 30 min.In group I + R,remifentanil (0.04 mg/kg,0.4ml) was subcutaneously infused for 30 min starting from the onset of skin incision.Paw withdrawal threshold to mechanical stimulation (PWMT) was measured at 24 h before operation and at 2,6,12,24 and 48 h after operation.After measurement of PWMT at 48 h,the rats were sacrificed and L4,5 segments of the spinal cord were removed rapidly to detect the expression of nuclear respiratory factor 1 (NRF-1) by immunofluorescence and Western blot.Results Compared with group C,PWMT was significantly decreased at each time point after operation,and the expression of NRF-1 in the spinal cord was up-regulated in I and I + R groups (P ＜ 0.05).Compared with group I,PWMT was significantly decreased at each time point after operation,and the expression of NRF-1 in the spinal cord was up-regulated in group I + R (P ＜ 0.05).Conclusion Up-regulation of NRF-1 expression in the spinal cord may be involved in the development of remifentail-induced hyperalgesia in a rat model of incisional pain.

Caenorhabditis elegans (C.elegans), has developed into an useful model for biomedical research, particularly in the targets of novel drugs and the related mechanism.C.elegans, combining with cost-effective cultivation, simple biological structure, make for high-throughput screenings in vivo model.In this review, we explore the feasibility of C.elegans in drug activity evaluation of complex systems.Because of the body independence of C.elegans, it provided good platform for the efficacy and mechanism of research on new drugs as well as Chinese herbal extract in vivo, showing a good prospect.

Objective To explore the effect of particulate matter (PM) 2.5 on the expression of pigment epithelium-derived factor (PEDF) protein in bronchial epithelial cells (BEAS-2B). Methods Human BEAS-2B were subcultivated, followed by low, medium and high concentrations of PM2.5 (25μg/ml, 50μg/ml, 100μg/ml) stimulation for 24 hours. The expression of PEDF protein in supernatant was ana-lyzed by enzyme-linked immunosorbent assay (ELISA), and the expression in BEAS-2B cells was detected by Western blotting. Results Compared with the control group, the expression of PEDF protein in supernatant and BEAS-2B cells induced by PM2.5 (25 μg/ml) in-creased, but no significance was found (t=-0.730, t=-1.840, P>0.05), and the expression induced by PM2.5 with the concentrations of 50μg/ml and 100μg/ml significantly increased (t>5.798, P<0.05). Conclusion PM2.5 with the concentrations of 50μg/ml and 100μg/ml could increase the expression of PEDF protein in a concentration-dependent manner both in supernatant and BEAS-2B cells.

Papillary thyroid microcarcinoma (PTMC) is a subtype of papillary thyroid cancer,and there are many debates about its treatments,including extent of thyroidectomy,necessity of prophylactic central-neck nodal dissection,risk-benefit ratio of thyroid-stimulating hormone suppression and indications of observation therapy,et al.The epidemiology and treatment programs of thyroid PTMC were reviewed in this article.