OBJECTIVE:To establish a method for the simultaneous determination of rhein,emodin,chrysophanol and phy-scion in Niuhuang shangqing pill. METHODS:HPLC was performed on the column was Kromasil C18 with mobile phase of metha-nol-0.5% phosphoric acid(75:25,V/V),the column temperature was 30 ℃,the flow rate was 1.0 ml/min,the detection wave-length was 230 nm,and the injection volume was 10μl. RESULTS:The linear range was 1.50-150 mg/L for rhein,0.95-95 for em-odin,1.30-130 for chrysophanol and 1.15-115 mg/L for physcion(r≥0.995 0);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 97.03%-100.40%(RSD=1.23%,n=9),98.37%-102.50%(RSD=1.15%,n=9),98.03%-101.10%(RSD=1.05%,n=9)and 97.83%-104.50%(RSD=2.16%,n=9). CONCLUSIONS:The method is simple,stable with good re-producibility,and can be used for the contents determination of rhein,emodin,chrysophanol and physcion in Niuhuang shangqing pill.

The paper discusses the subject service of clinical medicine in the age of big data , including two aspects:clinical research and clinical medical treatment .It points out that the development and utilization of medical big data require for subject librarians and doc -tors with certain information literacy and proposes suggestion for enhancing the information literacy of subject librarians and doctors .

Objective To investigate the mechanism of acupuncture in anti-depression. Methods 100 depressive patients were randomly divided into acupuncture group and Amitriptyline group, 50 cases in each group. They were respectively treated by acupuncture at Neiguan (PC6), Sanyinjiao (SP6), Baihui (DU20), Fengchi (GB20), and oral western meicine. The needles were manipulated once every 20 minutes and treatment was given once a day. HAMD score and levels of serum cell factors of two groups were observed. Results Serum levels of interleukin 1? (IL-1?), interleukin-6 (IL-6) interleukin-2 (IL-2) and tumor necorsis factor-? (TNF-?) in depressive subjects before treatment were significanly higher than that of nomal subjects (all P

Objective To discuss the application of insulation blanket in the esophageal cancer radical surgery.Methods 40 cases of esophageal carcinoma patients were divided into two groups at random,20 cases involved in the control group (non-use of insulation blankets) and 20 cases in the experimental group (use of thermal insulation blanket).The changes in body temperature of all patients were monitored with nasopharyngeal temperature probe and analyzed in statistics.Results Compared to the control group,the body temperatures of the experimental group were higher.By heating with insulation blanket,the body temperatures were increased obviously in 30 minutes after entering the operation room,but no significant difference appeared at different time points after 30 minutes.Conclusions In radical surgery of esophageal cancer,the use of blanket insulation is an effective way to maintain a constant body temperature of patients and reduce post-operative complications,and promote early recovery of patients.

Currently, the commonly used diagnostic markers have the shortcomings of lower sensitivity or specificity in the early diagnosis of cardio-cerebrovascular diseases. Therefore, it needs to use the novel specific biochemical markers for the early diagnosis arid treatment, so as to decrease the high mortality and disability caused by cardio-cerebrovascular events. Fatty acid-binding proteins (FABPs) are a family of low-molecular-weight intracellular lipid-binding proteins. They are divided into 9 different subtypes, including the liver-, intestinal-, heart-, brain-, adipocyte-, skin-, ileal-, myelin-, and testis-subtype. Their primary function is to transport long-chain fatty acids into the cells, and thus regulate intracellular lipid metabolism. The structures of all FABP subtypes are similar, inclusive of two α2 helices arid one β2 fold structure. Among them, the sensitivities and specificities of the heart- and brain-subtype FABPs are higher in the early diagnosis of cardio-cerebrovascular diseases. It is promising to become the novel markers in the early diagnosis of cardio-cerebrovascular diseases.

Environmental arsenic exposure is a worldwide public health problem. Long-term exposure to inorganic arsenic is closely related to diseases such as cardiovascular disease, diabetes and malignant tumors. Different dietary structure and intake of certain specific nutrients may have important effects on the pathogenic effect of arsenic. This article summarizes the effects of dietary nutrients on arsenic toxicity, providing reference for the follow-up arsenic toxicity research and reducing the risk of arsenic-related diseases through nutritional intervention.

Objective: To explore correlation among concentrations of high sensitive C reactive protein (hsCRP), uric acid (UA), brain natriuretic peptide (BNP) and coronary heart disease (CHD).Methods: A total of 120 CHD patients treated in our department were enrolled as CHD group, and they were divided into single vessel coronary disease group (single-vessel group, n=51) and multi-vessel coronary disease group (multi-vessel group, n=69).Meanwhile, another 40 healthy subjects were randomly selected from our physical examination center as healthy control group.Serum concentrations of hsCRP, BNP and UA were measured in all groups, and compared with pairwise comparisons.Results: Compared with healthy control group, there were significant rise in serum concentrations of hsCRP[(3.95±2.24) mg/L vs.(19.78±5.15) mg/L], BNP[(70.57±19.47) pg/ml vs.(196.17±54.84) pg/ml], UA[(184.51±79.47) μmol/L vs.(407.17±96.84) μmol/L]in CHD group, and those of multi-vessel group were significantly higher than those of single-vessel group, P<0.05 or <0.01;Spearman rank correlation analysis indicated that serum UA concentration was significant positively correlated with hsCRP and BNP concentrations (r=0.551, 0.398, P<0.05 both), Gensini score was significant positively correlated with serum concentrations of hsCRP, BNP, UA (r=0.478, 0.552, 0.741, P<0.05 all), Sullivan score was significant positively correlated with serum concentrations of hsCRP, BNP, UA (r=0.503, 0.674, 0.892, P<0.05 or <0.01) in CHD patients.Conclusion: Serum concentrations of hsCRP, BNP, UA are closely related to occurrence and development of CHD.These three indexes can be used as screening indexes for CHD, and the effect of combined detection may be better.

Objective:To discuss the rule of the prescriptions of professor JIANG Wen-zhao in treating the spleen and stomach ailment. Methods:Use the frequency analysis to analyze the 98 pieces of prescriptions of professor JIANG Wen-zhao in treating the spleen and stomach ailment. Results:To understand medicine state of professor JIANG Wen-zhao in treating the spleen and stomach ailment preliminarily,select the main medicine in treating diseases of the spleen and stomach,and make clear the significance of these medicines to the corresponding diseases. Conclusion:The basic rules of professor JIANG Wen-zhao in treating the spleen and stomach disease are regulating qi,removing dampness and reinforcing asthenia. Qi regulating herbs,diuretics for eliminating dampness,tonifying deficiency herbs,dampness-removing herbs,blood-activating and stasis-removing herbs,heat-clearing herbs are the key herbs.

Objective To observe the injury of myocardium and to investigate the role of oxidative stress after limbs ischemia reperfusion in rats. Methods 20 male healthy Wistar rats were randomly divided into 2 groups: control group(C) and ischemia-reperfusion group (IR). The biochemical methods were used to measure the contents of malondialdehyde (MDA), myeloperoxidase (MPO), ATP, lactic acid (LD) and ATPase of myocardium. The levels of creatine kinase (CK) and creatine kinase MB isoenzyme (CK-MB) in plasma were also observed. The morphologic changes of myocardium were observed with microscope. Results It was found that after rats’ limbs suffering from ischemia-reperfusion, the level of ATP decreased, the activities of three kinds of ATPase (Na~+-K~+-ATPase, Ca~ 2+ ATPase and Mg~ 2+ ATPase) decreased in different degree (P

Objective To study the imaging manifestations of parietal coronary artery and the diagnostic value with 64-slice spiral CT.Methods 1000 patients with clinically suspected coronary heart disease underwent 64-slice spiral CT scan,the images were dealed with curved planar reconstruction and analysed at workstation.Results Parietal coronary arteriae were found in 380 patients,among them,314 cases were at the LAD,32 cases were at the CX,13 cases were at the RCA and multiple arteriae involved in 21cases.Conclusion 64-slice spiral CT coronary angiography is an effective methed in diagnosis of parietal coronary artery.