OBJECTIVE:To prepare compound rifampicin auristilla and to establish a method for its quality control.METHODS:The content of rifampicin was determined by HPLC and the sample was stored under the temperature of(6?2)℃for6months.RESULTS:The linear range for rifampicin was6～60?g/ml,the average recovery was100.33%(RSD=1.23%).The appearance,pH value and contents of the preparation were in conformity with the requirements after storage for6months.CONCLUSION:This preparation method of compound rifampicin auristilla is simple and cheap,and its quality is controllable.

Objective The purpose of the present study was to explore the use of psychological intervention in modified electroconvulsive therapy patients.Methods Divide all 160 patients random into two groups,the intervened group and the contrast group. The intervened group was accepted psychological intervention before the therapy day;The contrast group just did as normal.Record the anxiety, blood pressure,heart rate and the agreement to the therapy of the two group before the therapy.Results The anxiety of the intervened group was much little to the contrast group.The blood pressure and the heart rate of the intervened group was lower than the contrast group.Patient of the intervened group was much active to match with the treatment. Conclusions The use of psychological intervention in modified electroconvulsive therapy patients could decrease patient’s anxiety and painness,made them more active to match with the treatment.

Angiotensin converting enzyme 2 (ACE2) is a new member of renin-angiotensin system found recently. It counterbalances the effects of angiotensin converting enzyme (ACE), hydrolyzes angiotensin Ⅱ to generate angiotensin-(1-7) and hydrolyzes angiotensin Ⅰ to produce angiotensin-(1-9). Angiotensin-(1-9) can be hydrolyzed by ACE to form angiotensin-(1-7), and angiotensin-(1-7) counteracts the effects of angiotensin Ⅱ in many aspects. Bradykinin or atrial natriuretic factor or substance P are not the substrates of ACE2, and ACE2 hydrolyzes the metabolites of bradykinin such as des-Arg~9-BK and des-Arg~(10)-kallidin, and also the dynorphin A, one of the opium peptides. ACE2 is not inhibited by angiotensin-converting enzyme inhibitor (ACEI). Recent studies demonstrate that ACE2 is a functional receptor of SARS coronavirus. To elucidate the mechanisms of cardiovascular diseases and other disease such as SARS, further investigations in ACE2 are needed.

Objective: To evaluate the satisfaction degree of non-medical students in medical school,and to investigate the factors that influence satisfaction degree. Method: Selfcompile scale is administered to 430 nonmedical students from 6 medical colleges,in which 5 is in Guangdong province and 1 is in Ha’erbin city. Result:The satisfaction of nonmedical students is low,significant diferences are found between grades,and between students with medicine as the first choice major and those whose first choice major is not medicine. Conclusion:Medical colleges should pay more attention to nonmedical majors,improve the quality of teaching,and so on in order to improve the nonmedical students’satisfaction degree.

Objective To observe the protective effects of the mixture of ferulic acid (AF) and paeoniflorin on PC12 cells injury by FeSO4/H2O2 in vitro, and explorer its mechanism. Methods The cell injury model was established with FeSO4/H2O2 for 24 h. The process of the growth and morphology of the cells were observed under phase contrast microscope in vitro. The survival rate of the cells was determined by MTT method. The content of MDA and SOD activity was measured respectively by thiobarbituric acid and xanthine oxidase method. Results Compared with FeSO4/H2O2, the mixture of ferulic acid (AF) and paeoniflorin enhanced the PC12 cells survival rate and viability, reduced MDA content and increased SOD activity. Conclusion The mixture of 5～50 ?mol/L AF and 1～10 ?mol/L paeoniflorin exerted protective effects on PC12 cells injury induced by FeSO4/H2O2, which maybe related to free radical scavenging activity and lipid peroxidation process being inhibited.

In this study we successfully prepared desmoglein 1 and desmoglein 3 DNA probes by incorporation of Dig-dUTP during PCR amplification. Eleetrophoresis analysis showed that Dig labelled probes moved slower on the gel than PCR products not incorporated with Dig due to the enlargement of probe fragment. The results of Dot-blot showed that the prepared probes can be used for RNA analysis, and both probes have high specificity, The results provided basis for the quantitative and qualitative study of desmoglein gene expression in tissues or cells by means of in situ hybridization and Northern hybridization etc.

It was shown that in the stricture on abdominal aorta (SAA) rat myocardiac hypertrophy model,the caudal blood pressure was increased,the wall of left ventricle was thichened, the proliferation of T cell in thymus and spleen was decreased and the content of IL-2 was less than normal. Acanthopanax giroldii polysaccharide (AGPS10mg/kg.d, sc) could harmonize the heightened blood pressure and increase the level of immunity in the body. It was suggested by the result, that AGPS could be used to strengthen the heart function as well as immunity.

Test on coagulation time,prothrombin time and platelet aggregation of mice and rats showed that all four species of Puhuang had effects on blood coagulation, in which Typha angustata Bory et Chaub. was the strongest followed by T. davidiana Hand. -Mazz., T. latifolia L,and T. angustifolia L. Their mechanism of coagulation were not the same,in which T. angustata Bory et Chaub. mainly acted on thrombogen,while T.davidiana Hand. -Mazz. mainly acted on platelet aggregation.

Objective Study changes and clinical significance of collagen and cytokine during COPD episode and remission in order to guide clinical treatment. Methods From Mar.2002 to Nov.2004,the COPD patients of episode and remission period were compared with the healthy elderly people.The collagen and cytokine of the serum from the COPD patients and healthy elderly people were detected by applying ABC-ELISA and radio-immunity means. Results The level of serum C_Ⅰ、C_Ⅲ、C_Ⅳ increased obviously in the COPD patients during COPD episode and remission episode compared with that in the healthy elderly people( P

Objective To discuss the role of IL-8 and NF-?B in the airway inflammation of COPD in stable stage.Methods From Mar.2003 to Sep.2005,select 26 COPD patients in stable stage,determine the concentration of IL-8 in the serum of induced sputum,separate the macropolycyte and determine the degree of active expression of NF-?B,the concentration of SOD,MDA and IL-8 in peripheral blood.Lung function(the percent of FEV_1/FEV_1 prospectived value,FEV_1/FVC)of all patients were investigated.Results The concentration of IL-8 in the serum of induced sputum of COPD patients was higher than the normal group obviously(P