Objective　To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods　Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used　　3　　H-TaR incorporation.Results　The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion　mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.

OBJECTIVE:To develop an HPLC method for simultaneous determination of calycosin-7-O-?-D-glycoside,ono-nin,calycosin and formononetin in Radix Astragali from eleven different habitats and to explore the internal factors of geoherbalism based on aspect of contents of active constituents. METHODS:The sample was separated on Zorbax Eclipse XDB-C18(250 mm?4.6 mm,5 ?m)column. The mobile phase consisted of acetonitrile-0.2% formic acid solution (gradient elution). The UV detection wavelength was set at 280 nm. RESULTS:The linear ranges of calycosin-7-O-?-D-glycoside,ononin,calycosin and formononetin were 0.005 1～0.510 mg?mL-1,0.005 0～0.300 mg?mL-1,0.004 9～0.294 mg?mL-1,0.004 6～0.276 mg?mL-1,respectively(r=0.999 9).Calycosin-7-O-?-D-glycoside and ononin took up a big proportion in Radix Astragali from Inner Mongolia and Shanxi; calycosin and formononetin took up a big proportion in Radix Astragali from Anguo,Chicheng of Hebei province and Dingxi of Gansu province. CONCLUSION:The method is simple,rapid and accurate. Results of study are in line with textual research on Radix Astragali.

Objective　To observe the effect of mycophenolate mofetil (MMF),a new type of immunosuppressant,on the immune function in patiens with systemic lupus erythematosus (SLE).Methods　The changes of serum lL-6,IL-8,TNF-α,sIL-2,ANA,A-dsDNA and subsets of lymphocytes were observed in patients with SLE before and after treatment with MMF and CTX by ELISA,indirect immunofluorescent assay and flow cytometry,and the effect of MMF and CTX on the functions of hematopoiesis,liver and kidney were also observed.Results　Three months after treatment with MMF,the serum levels of IL-6,IL-8,TNF-α,sIL-2,ANA,and A-dsDNA in patients with SLE were significantly decreased,CD3＋,CD4＋ and CD4＋CD45RA＋ cells were significantly increased,and CD8＋,CD4＋CD45RO＋,CD8＋CD45RA＋,CD8＋CD45RO＋ cells were significantly decreased.No remarkable injury was observed on the functions of hematopoiesis, liver and kidney after treatment with MMF.Conclusion　MMF can inhibit T and B lymphocytes selectvely and is a new type of immunosuppresant to treat SLE with less toxicity and side-effect.

Objective To investigate the early comprehensive intervention on neonatal hypoxic ischemic encephalopathy (HIE) effects and the biological mechanisms.Methods 120 cases of HIE were randomly divided into intervention group and control group.Under the basis of drug treatment,the intervention group were given the different perceptual stimuli,audiovisual stimulation,active and passive limb training early comprehensive intervention,the control group only to normal drug treatment.The mental development index(MDI) and psychomotor develo pmental index (PDI) were applied after 3,6,12,and 18 months.S100B protein levels were detected using enzyme immunoassay.Results MDI score in the intervention group with HIE,from the beginning of the 12 month,was higher than that in the control group,there was significant differences (12th month:(96.6±9.4)vs (86.8±9.5),P<0.01).PDI score from the beginning of the 6 month,was higher in the intervention group with HIE than that in the control group,there were significant differences (6th month:(91.6±9.2)vs (82.2±11.0),P<0.01).Serum S100B protein level has a tendency to fall for all HIE patients with the old growth.Born in two groups of serum S100B protein levels were higher in neonate period,but there was no difference(P>0.05).From the beginning of the 3rd month,serum S100B protein levels in the intervention group were reduced significandy,compared with the control group at each time point level(3rd month:(1.29±0.15)ng/L vs(1.40±0.14)ns/L;6th month:(1.06±0.21)ng/L vs(1.26±0.22)ng/L,P<0.01).Conclusion Early intervention can improve the mental and motor development of children with HIE,and serum S100B protein is involved in the mechanisms.

Objective To study the pathological foundations of enhanced MRI with superaramagnetic iron oxide(SPIO) and its diagnostic value in liver diseases.Methods 52 SD rats were divided into 2 groups in random,group A(n=40) bred with 2 ‰ diethylnitrosamine(DEN) to induce hepatocellular carcinoma(HCC),group B(n=12) as control group bred with water.6 rats and 2 rats in group A and group B were killed 6,8,11,14,17,20 week later,respectively,before that every SD rats underwent conventional plain and SPIO enhanced MRI examinations.All specimens were examined by HE staining and immunohistochemistry to test CD 68 with SP.Results The successful ratio of induced HCC was 90% and the mortality was 15%.The rats in group A could be divided into 4 stages,including hepatic fibrosis(HF),liver cirrhosis(LC),hyperplastic nodules and hepatocellular carcinoma(HCC).CD 68 positive cell in the normal control tissue(22.83?4.61/HP) was significantly more than that in LC(11.67?4.64/HP)(P

Aim To study the effects of HSP70 on hepatic ischemia-reperfusion injury after heat preconditioning in rats.Method To establish the models of the hepatic ischemia-reperfusion injury. 42 Wistar rats were randomly divided into 6 groups, (each group had 7 rats): normal group(N); quercetin injection group(Q); ischemia-reperfusion group(I); heat preconditioning 16 hours before ischemia-reperfusion group(H+I); quercetin injection before heat preconditioning group(Q+H+I); quercetin injection before ischemia-reperfusion group(Q+I).We detected the activity of serum enzyme of ALT,AST and the pathological changes of the liver;The expressions of HSP70 of the rats were observed by Western blotting. Results The expressions of HSP70 from high to low were:group H+I,group I,group Q+H+I,group Q+I,group Q,group N; The serum levels of ALT and AST from high to low were: group Q+I,group I,group Q+H+I,group H+I,group Q,group N;All groups had visibly hepatic histological changes respectively.Conclusion The protection of heat stress pretreatment from ischemia reperfusion injury was possibly performed by inducing the expression of HSP70.

Objective To explore the effects of alcohol on NO concentration in plasma and activity of ATP enzyme in erythrocytes of rabbits. Methods 32 rabbits were divided into groups and treated with alcohol, injected intravenously, at the doses of 0.5 g/kg, 1.0 g/kg and 1.5 g/kg, 15 minutes after treatment, the blood samples were collected and NO concentration in plasma, activity of ATP enzyme in erythrocytes were determined. Results NO concentration in plasma of rabbits significantly decreased in 0.5 g/kg and 1.0 g/kg groups, but increased in 1.5 g/kg group (P

Objective To investigate the early post-operative nutritional support for kidney transplant recipients.Methods Totally 67 patients who received early post-operative nutrition supports were randomly divided into three groups:group A (n = 24,relatively high nitrogen intake),group B (n = 25,intermediate nitrogen intake) and group C (n = 18,relatively low nitrogen intake).Two weeks after operation,albumin,hemoglobin,the nutrition indieators,serum lipids,and renal function 1 day before operation and 2 weeks after operation were evaluated. Results Body weight was significantly different among three groups;the nitrogen balance in group A was significantly better than those in groups B and C (P ＜0.05);albumin and hemoglobin were not significantly different between group A and group B (P ＞ 0.05),but were significantly higher than those in group C (P ＜ 0.05).The renal function in group C was significantly better than those in group A and group B (P ＜ 0.05).The triglyceride level was not significantly different among three groups,while the cholesterol level in group A was significantly higher than those in group B and group C (P ＜ 0.05),but not between group B and group C.Conclusion Proper nitrogen intake in early post-operative nutrition support can improve the recovery of renal function and nutritional status in kidney transplant recipients.

The 53rd ASH meeting was held in San Diego in December 2011. The meeting has received many advanced reports on the diagnosis and treatment of multiple myeloma (MM).On therapy,with the application of thalidomide, lenalidomide and bortezomib as inductive treatments, the clinical outcomeshave been improved greatly in MM patients.Pomalidomide,a new immunomodulator,can be hopefully used as a frontline medicine for MM because of its high response. Bendamustine combination with other treatments induced efficient response and good response rates in MM patients with renal insufficiency or relapsed/refactory disease. Currently autologous stem cell transplantation is still the standard care for newly diagnosed young MM patients.At this time other kinds of novel agents have entered into clinical trials and have shown a bright future of application.

Objective To evaluate the effect of heat treatment on ultraviolet B (UVB)-induced oxidative injury to human melanocytes.Methods Melanocytes were isolated from adult foreskins,and subjected to a primary culture.After 3-4 passages of subculture,the melanocytes were classified into 4 groups:control group incubated at 37 ℃,heat treatment group incubated at 42 ℃ for 1 hour,UVB group exposed to UVB irradiation at 100 mJ/cm2,combination group receiving heat treatment at 42 ℃ for 1 hour followed by UVB irradiation at 100 mJ/cm2.After three successive days of treatment,MTT assay was performed to evaluate cell viability,a biochemical method to determine the activity of superoxide dismutase (SOD) and concentration of malonaldehyde (MDA),and flow cytometry to detect intracellular reactive oxygen species (ROS) and apoptosis in melanocytes.Results The cell survival rate,apoptosis rate,SOD activity,MDA concentration and ROS level were (100 ± 6.14)％,(4.66 ± 0.58)％,(53.39 ± 8.23) U/gprot,(1.09 ± 0.32) mmol/gprot,and 1070.85 ± 42.07 in the control group respectively.UVB exposure induced a significant increase in apoptosis rate (24.14％ ± 2.90％,P ＜ 0.001),MDA concentration (1.65 ± 0.33 mmol/gprot,P ＜ 0.01) and ROS level (1416.45 ± 79.12,P＜ 0.01),but a significant decrease in cell survival rate (50.23％ ± 5.36％,P＜ 0.01)and SOD activity (31.98 ± 1 1.89 U/gprot,P ＜ 0.01) in the UVB group compared with the control group,while the heat pretreatment markedly downregulated the UVB-induced increase in apoptosis rate (14.9％ ± 1.49％,P ＜ 0.001),MDA concentration (1.10 ± 0.26 mmol/gprot) and ROS level (1033.30 ± 68.41,P＜ 0.01),as well as the decrease in cell survival rate (74.12％ ± 6.17％,P＜ 0.01) and SOD activity (51.63 ± 6.55 U/gprot,P＜ 0.01) in the combination group.Conclusion Heat treatment could protect melanocytes from UVB-induced oxidative injury.