Objective To investigate the MRI manifestations of cerebral glioma involving corpus callosum,and to enhance the ability to diagnosis and differential diagnosis.Methods Thirty four cases of cerebral glioma involving corpus callosum under went plain and Gd DTPA enhanced MR scans.The MRi findings of these gliomas were compared with operation and pathology.Results Gerebral gliomas involving corpus callosum presented as long T 1 and long T 2 homogeneous or inhomogeneous signal intensity and obvious mass effect.The MRI findings of Gd DTPA enhanced MR scans were obvious enhancement,slight enhancement or no enhancement accouding to different pathological kinds of gliomas."Butterfly signs" and "semi butterfly signs" were the reflections when gliomas of corpus callosum invaded two sides or one side of cerebral hemisphere.These two signs were significant markings in the diagnosis of cerebral gliomas in corpus callosum region.Conclusions Cerebral gliomas involving corpus callosum are intracranial tumors with special location.MRI has significant clinical value in the diagnosis and differential diagnosis of this kinds of tumour.

ObjectiveTo develop a analytical method of three phenoxyalkanoic acid herbicides,MCPA,2,4-D and dichlorprop,in grape leaf using dichloropropyl esterification and gas chromatography with electron-capture detection.MethodsSamples were pounded to pieces,added into internal standard 2,4-dichlorophenylacetic acid,acidified and extracted with ethyl ether.The extracts were cleaned up by liquid-liquid partition.The cleaned organic solutions were evaporated and the residues were dichloropropylated using sulfuric acid and 1,3-dichloropropan-2-ol.The formed esters were analysed by gas chromatography.ResultsThe detection limits of MCPA,2,4-D and dichlorprop in grape leaf were 20ng/g,20ng/g,and 30ng/g,respectively.The concentritions of herbicides in grape leaf were linear over the ranger 100~1000ng/ml.The average recoveries of MCPA,2,4-D and dichlorprop in spiked grape leaf at concentrition of 200ng/g were 98.8%,101.3% and 103.1% with the coefficients of variation 11.4%,11.0% and 9.3% respectively.ConclusionThis method is sensitive and accurate for determination of these three herbicides in poisoned grape leaf.

Objective To investigate the early post-operative nutritional support for kidney transplant recipients.Methods Totally 67 patients who received early post-operative nutrition supports were randomly divided into three groups:group A (n = 24,relatively high nitrogen intake),group B (n = 25,intermediate nitrogen intake) and group C (n = 18,relatively low nitrogen intake).Two weeks after operation,albumin,hemoglobin,the nutrition indieators,serum lipids,and renal function 1 day before operation and 2 weeks after operation were evaluated. Results Body weight was significantly different among three groups;the nitrogen balance in group A was significantly better than those in groups B and C (P ＜0.05);albumin and hemoglobin were not significantly different between group A and group B (P ＞ 0.05),but were significantly higher than those in group C (P ＜ 0.05).The renal function in group C was significantly better than those in group A and group B (P ＜ 0.05).The triglyceride level was not significantly different among three groups,while the cholesterol level in group A was significantly higher than those in group B and group C (P ＜ 0.05),but not between group B and group C.Conclusion Proper nitrogen intake in early post-operative nutrition support can improve the recovery of renal function and nutritional status in kidney transplant recipients.

BACKGROUND:Perioperative treatment of emergency liver transplantation for acute hepatic failure is extremely different from common liver transplantation, due to complex conditions, high risk, several complications, and high mortality. OBJECTIVE:To summarize the experience of emergency liver transplantation for acute hepatic failure during the perioperative period, and to increase the success rate in treatment of acute hepatic failure. METHODS:A retrospective analysis was undertaken on the clinical data of 38 cases undergone emergency liver transplantation for acute hepatic failure. There were 21 male and 17 female, who aged 15-69 years. Among them, 23 cases had hepatitis B virus (including 2 cases with hepatitis B and C virus), 7 cases had Wilsons disease, 3 cases had mushroom poisoning, 2 cases had unknown liver damage, 1 case had Tripterygium wilfordi poisoning, 1 case had decompensation after partial liver resection due to trauma, and 1 case had liver transplantation from corpse. RESULTS AND CONCLUSION:The survival time of the involve patients was 13-1 740 days, and the median survival time was 634 days. Perioperative survival rate was 76%, 1-year survival rate was 63%, and 2-year survival rate was 58%. During the perioperation nine cases died of brain edema and intracranial hypertension, renal failure, severe pulmonary infection, multiple organ failure, coagulation disorders (intracranial hemorrhage, upper digestive tract hemorrhage), acute respiratory distress syndrome and primary graft non-function. At present, emergency liver transplantation is stil the most effective way for acute liver failure. Hemorrhage, infection and rejection are the leading causes of the death. Each perioperative treatment is of great significance for the success of liver transplantation and long-term survival.

Objective To analyze the correlation between 18F-FDG SUV and immunohistochemical index including GLUT1, Ki-67, MVD, survivin and cyclinA in non-snall-cell lung cancer. Methods Thirty-three patients with NSCLC underwent preoperative PET/CT examination and surgical operation. All patients were divided into two groups according to the size of tumor (cutoff=3 cm), metastasis of mediastinal or hilar lymph nodes or not, and histological types of the cancer, respectively. The expression of GLUT1, Ki-67, MVD, survivin and cyclinA were estimated with SP immunohistochemical technique, and were analyzed statistically to reveal the correlation to FDG SUV. Results The rate of positive expression of GLUT1, Ki-67 and CD34 were 66.67%, 72.73% and 100%, respectively. The mean value of CD34 in all 33 patients was 12.6±2.9 (12-56). The rate of positive expression of survivin was 84.85%, and the corresponding data of cyclinA was 27.27%. Conclusion There is linear correlation between FDG PET SUV and GLUT1, but not between FDG PET SUV and Ki-67, MVD, survivin and cyclinA. The expressions of GLUT1, Ki-67, MVD, survivin and cyclinA are not related with the size of tumor, nor metastasis of lymph nodes. The expression of GLUT1 and Ki-67 is related with histological types of the cancer, but not with MVD, survivin and cyclinA.

Objective To investigate the value of nuchal translucency(NT) thickening in the fetal chromosome abnormality screening.Methods The 14 881 pregnant women received NT measurement in 11-13 +6 weeks at Beijing Obstetrics and Gynecology Hospital from January 2010 to August 2012.The 118 fetuses whose NT≥2.5 mm were recruited.One hundred and eight (91.5％,108/118)of them accepted invasive procedure and karyotype analysis.Results (1) Chromosome karyotype analysis:113 singleton pregnancies (95.8％) and 5 twin pregnancies (4.2％) whose NT thickened from 2.5 mm to 11.0 mm were advised karyotype analysis.The 108 pregnant women accepted karyotype analysis.Among them,88 had normal chromosome karyotype,and 20 had chromosome abnormalities.The detection rate was 18.5 ％ (20/108).(2) The sensitivity,specificity,false positive rate,false negative rate,total consistent rate,positive predictive value and negative predictive value of prenatal screening of chromosome abnormalities for NT ≥ 2.5 mm were 44％,99％,1％,56％,99％,19％ and 100％,respectively.(3) Among the 88 fetuses who had normal karyotype,72 (82％) had isolated thickened NT,while 16 (18％) had fetal structural malformation or intrauterine demise.(4) Among the 10 pregnant women who did not accept fetal karyotype analysis,8 terminated pregnancy because of fetal structural malformation,and the other 2 fetuses died in uterus.(5)All of the 5 twin pregnancies were dichorionic twins,and one of the twins had thickened NT.Among the 5 twin pregnancies,one fetus was trisomy 21 and others had normal karyotype.3 twin pregnancies who had normal chromosomes gave live birth.And the other had a gastroschisis and exstrophy deformity fetuse.This fetuse died in uterus and remaining fetuse had full-term live birth.(6) 35 (29.7％) pregnant women received second trimester Down's syndrome serum screening.One was high risk and was proved a trisomy 21 pregnancy.Thirty-four were low risk and had normal chromosome karyotype.Conclusion NT thickening may indicate fetal chromosome abnormalities or other structural malformations.It is a useful prenatal screening indicator.

OBJECTIVE To develop technique of central vein catheterization by external jugular vein puncture under ultrasound guidance.METHODS A total of 224 patients with central vein catheterization by external jugular vein puncture were randomized into two groups: the control group was treated by routine manipulation and observed blood vessels by macroscopic observation;the patients from the observation group were placed catheters with the assist of ultrasound techniques.To record the one time achievement ratio of catheterization and incidence of phlebitis and thrombus.RESULTS Comparing the two groups,we found the observation group had higher one time achievement ratio of catheterization and lower incidence of phlebitis and thrombus than that of the control group.And there were statistical significances between the two groups(P

OBJECTIVE To analyze the clinical therapeutic effect of a disposable vaginal adsorber for Weiyinkang and lacidophilin vaginal capsules(Yanhua) on bacterial vaginosis.METHODS A total of 140 patients were divided randomly and averagely into the group A and the group B.The two groups were treated respectively by metronidazole or Weiyinkang combined with lacidophilin vaginal capsules.RESULTS The effective rate of the groups B and A was 92.8% and 80.0%,respectively,with significant difference(?2 =4.93,P

BACKGROUND: Twin-block appliance has been confirmed to efficiently change hard tissue and the profile of soft tissue.Whether a linear relationship exists between soft tissue and hard tissue changes remains unclear.OBJECTIVE: To evaluate the face convexity changes of soft and hard tissues after Twin-block appliance therapy and compare with controls.METHODS: Thirty-one class Ⅱ division 1 malocclusion subjects with hand-wrist radiographs in FG-G stage were selected.Among the 31 patients,17 received Twin-block appliance therapy,serving as experimental group,and the remaining 14 subjects abandoned treatment,serving as control group.Cephalometric radiographs of each included subject were taken prior to and after treatment or observation.Face convexity of soft and hard tissues,as well as the face convexity changes,was compared between the experimental and control groups.Linear regression equation was employed to analyze the linear association between soft-and hard-tissue changes.Regression equations of experimental and control groups were compared.RESULTS AND CONCLUSION: Prior to and after Twin-block appliance therapy,a significant correlation existed between the position changes of superior and inferior alveolar sockets relative to the nose and mandible,and the position changes of upper and lower lip pits relative to the nose and mandible,i.e.,there was a linear correlation between A-E change and Ss-E change,and between B-E change and Si-E change.The largest Pearson's correlation coefficient(0.839)appeared between Si-E change and B-E change,indicating the best correlativity between these two changes.Statistical analysis revealed that the linear equations of face convexity changes of soft and hard tissues after Twin-block appliance therapy were Si-E=0.745 B-E,Ss-E=0.276 A-E.These linear equations would be helpful to explain mandible growth and face convexity change after Twin-block appliance therapy and predict the prognosis of face convexity change.

Objective To study the expression levels of SCCA1 and SCCA2 mRNA in tissues of cervical squamous cell carcinoma. To investigate the role of this gene in the clinical diagnosis, evaluation of treatment and observation of prognosis of cervical squamous cell carcinoma. Methods Quantitative real-time RT-PCR was used to detect the expression of SCCA1 and SCCA2 mRNA in tissues of 60 cases of cervical squamous cell carcinoma and those of 30 cases of normal cervical tissues. Results The expression level of SCCA2 mRNA in tissues of 30 cases of cervical squamous cell carcinoma was higher than in those of 15 cases of normal cervical tissues (4.405 ± 2.310, 9.088 ± 2.195) (t =-6.513, P ＜0.001), while the expression level of SCCA 1 mRNA did not significantly differ between normal and malignant tissues (P ＞0.05). The expression of SCCA2 mRNA was relevant to FIGO stages and there was a tendency for this gene to increase with the stage getting worse (F =8.313, P ＜0.05). Moreover, the overexpression of SCCA2 mRNA was significantly correlated with lymph node metastases (t =2.853, P ＜0.05). The expression of SCCA2 mRNA was not correlated with age and pathological grading (P ＞0.05). However, the expression of SCCA1 mRNA was not correlated with age,FIGO stages, lymph node metastases and histological grade (P ＞0.05). Conclusion The expression of SCCA2 mRNA may provide help for more accurate diagnosis on the clinical stages and lymph node metastases of cervical squamous cell carcinoma.