Objective: To reasonably allocate the hospital fixed assets, improve the use efficiency of hospital fixed assets and enhance the management level of hospital fixed assets. Methods: Literature method, observation method and expert interview are mainly adopted to launch comprehensive investigation on the current management conditions of the fixed assets of public hospitals in Quzhou from 2009 to 2011. Results: The allocation of assets is not reasonable, the utilization rate of large devices is not high and the return on assets is relatively low. Conclusion: Professional centralized management of large special equipment should be implemented, informatization management is comprehensively implemented, ABC management approach is applied to conduct classified management of fixed hospitals of the hospitals, so as to establish layered assets management accountability system.

Objective To explore the effects of the multidisciplinary cooperative continue nursing on the quality of life of patients with chronic obstructive pulmonary disease (COPD). Methods From December 2012 to December 2014, hospitalized COPD patients who were in the stable stage of disease and would soon be discharged and met the criteria standards were chosen. Convenient extraction of 64 patients were set as the experimental group in one ward and 50 patients as the control group in the other ward. The conventional discharge guidance was employed in the control group. While the continuation of care before discharge and 1, 3, 6 months after discharge was employed in the experimental group. The indexes such as the quality of life and 6 min walking test distance (6MWD) were observed and compared. Results The total scores and symptoms, activities, influence scores of the quality of life were 48.53±15.78, 35.38± 18.61, 57.95 ±23.69, 52.28 ±15.27 after 6 months intervention in the experimental group and 60.24 ±10.14, 52.76 ±12.36, 68.34 ±15.59, 58.55 ±11.79 in the control group, and there were significant differences (t=-5.69--2.38, P<0.05). 6MWD was (267.46 ±64.64) m after 6 months intervention in the experimental group,which was more than that before intervention (169.42±48.46) m, and there was significant difference (t=-9.71, P<0.01). 6MWD was (201.32±53.93) m after 6 months intervention in the control group, which was not significantly better than that in the experimental group (t=5.82, P<0.01). Conclusions The quality of life of patients with COPD can be significantly improved by the multidisciplinary cooperative continue nursing education after discharge.

Objective To investigate the protective effects and mechanisms of NF-KB inhibitor pyrrolidine dithiocarbamate (PDTC) on infant rabbits with lung injury caused by mechanical ventilation. Methods Twenty healthy infant rabbits were randomly divided into four groups. (1) Mechanical ventilation (MV, with VT = 24 ml/kg);(2) Mechanical ventilation plus PDTC pre-treatment (MVP, VT = 24 ml/kg with PDTC 100 mg/kg injection via ear vein half an hour prior to MV) ; (3) Mechanical ventilation combined with endotoxin (EMV, 0.1 ml/kg of endotoxin dripping into trachea then on MV, VT = 24 ml/kg) ; (4) EMV plus PDTC pretreatment (EMVP, PDTC 100 mg/kg injection via ear vein followed by 0.1 mg/kg of endotoxin dripping into trachea in half an hour then on MV with VT = 24 ml/kg for 4 h continuously. MPO and the activation of NF-κB in lung tissues and the genetic expression and protein quantity of TNF-α and IL-8 in homogenate were measured. The pathological changes in lung tissues were examined. Results Pre-treatment with PDTC had significant minor pathological changes caused by MV and MV plus endotoxin, which were indicated by the fact that MPO, activation of NF-KB and the genetic expression and protein quantity of TNF-α and IL-8 were significantly suppressed. Conclusions PDTC could decrease the expression, synthesis and release of pro-inflammatory cytokines. This may be through suppressing the activation of NF-κB resulting in less infiltration with inflammatory cells and protective effects on lung injury caused by MV and MV plus endotoxin.

Objective To expore the mechanism of low-dose interfone-γ(IFN-γ) influences on differentiation of oligodendrocyte precursor cell. Methods The cerebral cortex samples were obtained from one day old SD rats to form mixed single cell suspensions. After culturing in full medium for 7 to 10 days, succession and differential velocity adherent technique were performed to acquire oligodendrocyte precursor cell and cultured in serum-free medium. IFN-γ, AG490 and Fludarabine were added during the culture of oligodendrocyte precursor cell and reverse transcription-polymerase chain reaction, Western blot and flow cytometry were performed to evaluate the expression of intracellular P27kip1 and its influence on the differentiation of oligodendrocyte precursor cell. Results (1)The expression of P27kip1 mRNA and protein was lower in IFN-γ group than in control group (t=85. 535, P<0. 05;t= 12. 481, P<0. 05), while the expression of P27kip1 mRNA and protein in IFN-γ+AG490 group and IFN-γ+Fludarabine group were both higher than those in IFN-γ group (P<0. 05). (2) The phosphorylation levels of JAK2/STAT1 in INF-γ group were higher than that in the other three groups (P<0. 05). (3) The percentage of myelin basic protein positive cells was (68. 42 ± 2. 53)% in IFN-γ group, lower than that in control group [(88.21 ± 1.97)%](t=10.682, P < 0.05). Myelin basic protein positive cells in IFN-γ + AG490 group were (57. 63 ±2. 75) %, lower than those in the IFN-γ group. The same figure in IFN-γ+Fludarabine group were (79. 53±4. 15)% , higher than those in IFN-γ group (t = 3.957, P<0.05). Conclusions Low-dose IFN-γ can regulate the expression of intracellular P27kip1 through JAK2/STAT1 signal transduction pathway and Fludarabine may participate in this process and improve the differentiation and maturation of oligodendrocyte precursor cell.

Objective To detect the development of fetal nasal bone with ultrasound scan in the second and third trimester, and to evaluate the value of fetal nasal bone hypoplasia in diagnosing fetal chromosomal abnormalities. Methods Prenatal ultrasound examination was performed in 1415 normal fetuses. The nasal bone length (NBL) and the nasal bone angle (NBA) were measured on the mid-sagittal plane. The karyotype of the cases with nasal bone abnormality was analyzed. Results The fetal nasal bone was successfully detected with two-dimensional ultrasonography in 95% of the subjects. The NBL increased with the gestational age while the NBA kept unchanged with the average value of 54. 4?(44. 4?-69. 2?). Forty-two cases with nasal bone hypoplasia were diagnosed prenatally including nasal bone defect (20 cases), small NBA (15 cases), short nasal bone (4 cases) and short nasal bone with small NBA (3 cases). Cordocentesis was performed in 21 cases among which 12 with abnormal karyotype, including 3 cases of trisomy 21, 3 cases of trisomy 13, 4 cases of trisomy 18 and 3 other chromosomal abnormalities. Conclusions Fetal nasal bone hypoplasia was closely related to the chromosomal abnormalities. The evaluation of fetal nasal bone with prenatal sonography is an important criteria for screening fetal chromosome abnormalities during the mid-trimester.

Background:Faecalibacterium prausnitzii( Fp) is one of the most abundant bacterium in human intestinal microbiota,and is closely correlated with the process of colitis-associated colorectal cancer(CAC). Aims:To observe the effect of Fp on CAC,and investigate the possible mechanism. Methods:The model of CAC was induced by azoxymethane (AOM)and dextran sodium sulfate( DSS). Fifty-two C57BL/ 6J mice were randomly divided into 4 groups:group A (AOM + DSS),group B(AOM + DSS + Fp),group C(AOM + DSS + Fp supernatant)and group D(control group). All the mice were sacrificed on day 92. DAI was assessed,serum levels of TNF-α and IL-10 were determined by ELISA. HE staining was used to examine the grade of tumor. Expressions of VEGF,COX-2,NF-κB in tumor tissue were measured by immunohistochemistry. Results:The tumorigenesis rates of group A,B,C were 100% ,100% and 77. 8% ,respectively;mainly were high-grade intraepithelial neoplasia. The tumor load in group A was significantly higher than that in group B (P < 0. 01),and the spleen index in group B was significantly higher than that in group C(P < 0. 01). Serum level of TNF-α was significantly lower(P < 0. 05)and IL-10 was significantly higher(P < 0. 05)in group A than that in group B. No significant differences in expressions of VEGF,COX-2,NF-κB were found among group A,B and C. Conclusions:Fp had no obvious effect on the occurrence rate of CAC,and Fp supernatant could decrease the incidence of CAC in mice. Fp and its supernatant could reduce the tumor load via regulating the expressions of TNF-α,IL-10.

AIM To establish a UV spectrophotometry for the determination of arsenic in Jiuji Xingjun Capsules. METHODS Arsenic was transformed into hydrogen arsenide,then silver diethyldithiocarbamate was reduced for colloid silver by hydrogen arsenide,its absorbance was determined at the wavelength of 507 nm. RESULTS The linearity range of curve was 0.5-4.5 ?g?mL -1 ( r =0.999 5, n =7),The average recovery was 100.11 % ( RSD =1.64 T《B》 % , n =15). CONCLUSION This method is rapid and accurate.

Aim: To enhance the management of clinical teaching hospital and improve the quality of clinical teaching.Methods: The criteria system of evaluation on teaching hospitals were taken to assess the teaching quality of 4 non-directly affiliated hospitals by means of listening to the self-evaluation,checking the file,surveying on the scene and holding student symposium.Prompt feedback were supplied to the 4 hospitals.Result: The 4 hospitals obtained similar scores,which had intelligent teachers,favorable teaching condition,strict management and better teaching quality.Conclusions: Emphasizing teaching,qualified teachers,improving teaching condition and optimizing teaching method are the important measures of improving clinical teaching quality.

Exploring a pattern of teaching hospital evaluation to solve the problem of its present teaching hospitals being not enough to meet the needs of undergraduate education in the coming a couple of years due to the adjustment of enrolling system and the increase of student number .Through the evaluation ,the university is intended to attain the goal of promoting the building and development in medical treatment,teaching and research of all kinds of teaching hospitals,and the goal of "promoting the development and improvement through evaluation ,combining the evaluation with development ".

Objective To investigate the expression of placental growth factor (PlGF) protein and mRNA in lungs of neonatal rats exposed to 85%hyperoxia, and to establish the relationship between PlGF and bronchopulmonary dysplasia (BPD). Methods Forty-eight Sprague–Dawley neonatal rats were randomly exposed to air (control group)(n=24) and 85% hyperoxia (hyperoxia group)(n=24)within 12 h after birth. The rats were sacrificed at 3, 5 and 7 days after exposure (eight at each time) and their lungs were sampled. PlGF protein and mRNA expression in the lungs were determined by Western blot and real-time polymerase chain reaction (PCR) at 3, 5 and 7 days. Left lung tissue was used for morphological and histological observation with hematoxylin and eosin staining. Terminal air spaces and the secondary septa were counted manually under microscope. T-test was applied for statistics. Results Compared with the control group, morphological and histological analysis in the hyperoxia group revealed inflammatory cell infiltration, simplified alveolar structure, less alveolar, alveolar cavity expansion and thickened alveolar septum. Morphometric measurements showed that terminal air spaces and secondary septa were significantly fewer in the hyperoxia rats than those in the control group at 5 and 7 days (terminal air spaces:23.6±8.2 vs 33.1±6.2 and 28.5±9.2 vs 38.4±10.1, t=1.91, 2.53, all P<0.05;secondary septa:56.0±12.2 vs 78.3±8.2 and 75.4±12.2 vs 126.1±10.2, t=2.14, 2.72, all P<0.05). Real-time PCR showed that expression of PlGF mRNA increased significantly on day 3, 5 and 7 in the hyperoxia group compared with the control group (1.16±0.17, 1.34±0.15 and 1.65±0.19 vs 0.65±0.21, 0.47±0.11 and 0.46±0.17, respectively, t=1.93, 2.55, 2.79, all P<0.05). Western blot also showed that expression of PlGF protein on day 3, 5 and 7 in the hyperoxia group increased compared with the control group, but only being significant on day 3 (0.24±0.17 vs 0.09±0.01, t=2.44, P<0.05). Conclusions Hyperoxia (85%) exposure could increase PlGF protein and mRNA expression in the lungs of neonatal rats, likely contributing to pathogenesis of BPD, and might lead to pulmonary vascular developmental disorders in BPD.