Objective To study the expression of iNOS and AChE during streptomycin ototoxicity and the correlation.Methods 16 guinea pigs were divided into 2 groups randomly:control group and SM group.SABC immunohistochemical staining and image quantitative analysis technique were used to observe the expression of iNOS and AChE and grey value analysis.ABR measurements were used to monitor the effects of ototoxity.Results After 10 days with drugs,the ABR threshold value of SM group increased more significantly than that of the control group(P

Aim To examine the apoptosis and bFGF expression on the cochlea of guinea pig after administrated streptomycin(SM) and the antagonism of Salvia Miltiorrhiza Injection(DS) on SM ototoxity.Methods Forty guinea pigs were divided into four groups,and treated with DS and SM respectively.The apoptosis and bFGF expression were examined by TdT mediated biotin dUTP nickend labeling(TUNEL) techniques and SABC immunohistochemical staining with paraffin slide.The intensity of images were stored in a computer and analyzed by the image quantitative analysis technique.Auditory brainstem response(ABR) measurement was used to detect the ototoxicity before/after the examination.Results After 10 d treated by drugs,the ABR threshold of SM increased significantly,while the DS+SM reduced more(P

Objective To observe the clinical efficacy of mesalazine and bifid-triple viable combination therapy in patients with ulcerative colitis (UC).Methods Thirty-eight UC patients were evenly divided into mesalazine group and combination group. Patients in mesalazine group were administrated with mesalazine (1.0 g/time,four times/day),and on the basis of this,the patients in combination group were added into bifid-triple viable (420 mg/time,three times/day).The course of treatment was eight weeks.The improvement of patients' clinical symptom and manifestation under colonoscope were observed at second day after hospitalization and eight weeks after medication.Mayo disease activity index was used for scoring.Before and after the treatment,the expression of intestinal mucosa nuclear factor kappa B (NF-κB),tumor necrosis factor-α (TNF-a) and cyclooxygenase-2 (COX-2) were tested by immunohistochemical staining and the levels of serum TNF-α,interleukin-8 (IL-8), interleukin-10 (IL-10) were determined by double antibody sandwich enzyme-linked immunosorbent assay.The level of serum superoxide dismutase (SOD) and malondialdehyde (MDA) was detected by nitrite method and thiobarbituric acid coloration method.Before and after treatment was compared with paired t-test,and qualitative data was analyzed with ordinal rank sum test.Results Mayo disease activity index score of the combination group decreased from 7.16±2.01 to 1.63± 1.30 while the mesalazine group from 7.42 ± 1.95 to 3.00 ± 1.25.The difference of reduction was statistically significant (t =2.093,P =0.043).The efficiency of the combination group was higher than that of mesalazine group (16/19 vs 10/19,Fisher's exact test,P=0.039).After treatment,the expressions of NF-κB,TNF-α and COX-2 in colonic mucosa of two groups were both lower than those of before treatment,and in combination group the decrease were more significant (t value was 2.262,2.607 and 2.522 respectively,P value was 0.027,0.012 and 0.014 respectively).After treatment,the concentration of IL-8 of mesalazine group and the combination group both significantly decreased,however IL-10 significantly increased. The difference between these two groups was statistically significant (t value of TNF-α,IL-8 and IL-10 was 4.244,6.858 and 6.802 respectively,all P＜ 0.01). After treatment,both SOD vitality of two groups increased compare with that before treatment,however the level of MDA was lower than that before treatment and the changes of combination group were more significant.Conclusion The combination therapy with mesalazine and bifid-triple viable can effectively suppress inflammatory response in mild and moderate ulcerative colitis,and bifid-triple viable may play a role as adjuvant therapy for patients with UC.

Objective To explore the effect of histamine in paraventricular nucleus (PVN) on the development of neurogenic pulmonary edema (NPE).Methods NPE models of rabbits were made and H 1 receptor antagonist (chlorpheniramine, H 1 group), H 2 receptor antagonist (Cimetidine, H 2 group) or normal sodium (NS group) were injected into PVN of rabbits utilizing the technique of stereotaxis. The degree of pulmonary edema was observed, the content of histamine in PVN was measured by high performance liquid chromatogram, and the ration of lung-water and A/P value were calculated.Results (1) Compared with control group and sham operation group, the content of histamine of PVN in NPE group obviously increased ( P

Objective To develop a satisfactory and reliable method for simultaneous determination of seven cephalosporin antibiotics (cefadroxil,cephalexin,cefoperazone,cefuroxime,cefradine,ceftazidime and cefotaxime) in water samples using solidphase extraction and high performance liquid chromatography.Methods The detection and reference wavelengths were 254 and 270 nm,respectively.The analytes were separated within 20 min by a gradient elution program.The optimized pretreatment of water samples was as following:using hydrophilic-lipophilic balance HLB as SPE cartridges,adjusting pH to 3 and adding 3.0 g NaCl per 500 ml water sample.Results The linear range of the method was 0.05-5.00 mg/L for ceftazidime,cefotaxime and cefuroxime,0.10-10.00 mg/L for cefadroxil,cephalexin and cefradine,and 0.20-20.00mg/L for cefoperazone.The coefficients for the analytes were above 0.99 and the limits of determination were 0.05-0.39 ?g/L.The recoveries of seven cephalosporin antibiotics in purified water were 86.64%-105.28%,and its relative standard deviation were 2.61%-11.64%.Conclusion The method was sensitive,accurate and repeatable,it is applicable to the determination of the cephalosporin antibiotics in waters.

Objective To study the effect of noise on the ability of learning and memory of mice,and observe the effect of herba Houttuyniae injection on the change of the ability of learning and memory in noise condition, and then analyses the mechanisms. Methods To choose suitable mice and divide into groups, applying Y-maze and colorimeter to determine the content of MDA and the enzyme activity of SOD, AChE and NOS of mice brain. Results Learning group in Y-maze, noise cause the ability of noise group (12.1±1.20) decrease(P＜0.01) compared with the control group(15.1±1.45), but herba Houttuyniae injection can reverse the ability of the mice, the correct frequency is much higher(P＜0.01)in herba Houttuyniae group(14.9±1.60). The results is same in the memory group, in Y-maze, noise cause the memory ability of noise group (5.20±1.40) decrease(P＜0.01) compared with the control group(6.30±1.32), but herba Houttuyniae injection can reverse the memory ability of the mice, the correct frequency is much higher(P＜0.01)in herba Houttuyniae group(6.10±1.10). Either learning or memory group mice, noise can decreased the activity of SOD and NOS of mice brain(P＜0.01), but the content of MDA and the ability of AChE elevated(P＜0.01). herba Houttuyniae injection can change the effect of noise to mice, the activity of SOD and NOS of mice brain elevated(P＜0.01) and the content of MDA and the ability of AChE decreased (P＜0.01). Conclusion herba Houttuyniae injection can prevent the ability of learning and memory of mice which decreased by noise, and have the effect of intoxication.

Objective To observe the effect of tetraethylplyrazine(TMP) on outward K~+ channel of outer hair cells in guinea pig cochlea.Methods 60 guinea pigs were divided into 6 groups randomly in the experiment.Auditory brainstem response was used to monitor the change of ABR thresholds and patch clamp techniques to observe the effect of TMP on outward K~+ channel.Results TMP decreased the elevated ABR threshold caused by streptomycin. The TMP increased the amplitudes of calcium sensitive potassium channels (I_ K(Ca) ) and delayed outward potassium channels (IK) of outer hair cells of guinea pig cochlea.Conclusion The study indicates that TMP may act as a protective agent against ototoxicity of streptomycin. The amplitudes of I_ K(Ca) and IK of outer hair cells are increased by the TMP,suggesting the possible mechanisms of reducing ototoxicity.

Purpose To investigate the expression of Netrin-1 in ovarian serous carcinoma and its c1inicopatho1ogica1 significance. Methods Netrin-1 protein expression was assessed by immunohistochemica1 method in tissue specimens from 20 cases of benign ovari-an serous cystadenomas,13 cases of border1ine ovarian serous neop1asms and 32 cases of ovarian serous carcinomas( OSC). Results The positive proportion of Netrin-1 protein in OSC tissues was significant1y higher than those in border1ine and benign ovarian serous ne-op1asms(P<0. 01). The expression of Netrin-1 in OSC tissues was associated with tumor grade and c1inica1 stage(P<0. 05),but not associated with age,site,tumor size or 1ymph node metastasis( P>0. 05). Kap1an-Meier ana1ysis showed that the 5-year surviva1 rate of patients with Netrin-1 over-expression was significant1y 1ower than that of patients with 1ower expression( P<0. 05 ). Conclu-sions The high expression of Netrin-1 in OSC tissues indicates that Netrin-1 p1ays an important ro1e in cancer pathogenesis and deve1-opment and it may be a new assistant marker for prognosis of OSC.

Objective To investigate the expression of inducible nitric oxide synthase(iNOS) in the cochlear stria vascularis(SV) of guinea pigs after streptomycin(SM).Methods Auditory brainstem response(ABR) was used as measurement index,and combining with transmission electron microscope(TEM),immunohistochemical staining and image quantitative analysis technique, to detect iNOS expression in the cochlea stria vascularis of guinea pigs and the change of morphology after SM.Results 10 days after adminitration, ABR threshold of SM group increased more significantly than that of the control group(P

Objective To evaluate the diagnostic accuracy and integrity of two-dimension ultrasound (2DUS)assisted with spatio-temporal image correlation (STIC)for cardiac and vascular abnormalities in fetal heterotaxy syndrome (HS).Methods The retrospective study was conducted from Nov 2007 to Feb 2013.Forty-five fetuses with suspected heterotaxy syndrome on routine prenatal ultrasonic screening underwent STIC volume sweep.The diagnosis was confirmed by pathological or echocardiographic examination.STIC volume data sets of fetal hearts were off-line analyzed blindly by one doctor who had practiced fetal echocardiography for more than five years.The concordance and integrity levels of diagnosis with 2DUS or assisted with STIC were compared according with pathological results.Results There were 397 cardiac and vascular defects in 37 cases of right isomerism.The overall concordance of various defects in right isomerism diagnosed assisted with STIC (96.5%)was significantly higher than that of 2DUS (60.2%) (P <0.05).The concordance rates of right atria and ventricle isomerism,anomalies of ventriculo-arterial junction,arterial arch,systematic and pulmonary veins diagnosed assisted with STIC (78.9%,96.7%, 100%,100%,100%)were higher(P <0.05)than those of 2DUS (49.1 %,60%,34.7%,23.2%,62.2%). Percentages of diagnostic integrity levels (0 ~3)on 2DUS+STIC (8% on level 2,92% on level 3 )were significantly different(P <0.05)with those on 2DUS (1 1 % on level 0,21 % on level 1 ,57% on level 2, 1 1 % on level 3).There were 59 cardiac and vascular defects in 8 cases of left isomerism with the overall concordance of defects diagnosed assisted with STIC (89.8%)and 2DUS (81 .4%)being no difference (P >0.05).There was no significant difference in concordance rates of various defects of left isomerism diagnosed between two methods,except that the concordance rate of left atria isomerism diagnosed assisted with STIC was higher(P <0.05).There was no difference in percentages of diagnostic integrity levels (0-3)in left isomerism between two methods.Conclusions STIC technology may provide considerable diagnostic information for ultrasonic diagnosis of fetal heterotaxy syndrome,especially in abnormalities of atria and ventricle isomerism,ventriculo-arterial junction,arterial arches,systematic and pulmonary veins in right isomerism.