Objective To investigate the role of CD4+CD25+regulatory T cells and CD4+CD25gigh regulatory T cells in peripheral blood and decidua in the pathogenesis of intrahepatic cholestasis of pregnancy (ICP).Methods Peripheral blood and decidua CD4+CD25+regulatory T ceHs and CD4+CD25high regulatory T cells in 30 ICP patients(15 mild ICP and 15 severe ICP)and 28 normal pregnant women were analyzed by flow cytometry.Results The percentage of CD4+CD25+ regulatory T cells and CD4+cD25high regulatory T cells in the CD4+T cells population in both peripheral blood and decidua in ICP patients were significantly lower than those in control women[(7.96±1.32)%vs(17.05 ±2.86)%,(17.18±2.27)%vs(32.01±3.88)%;(0.78±0.22)%vs(1.71±0.69)%,(2.25±0.89)%vs(8.30±1.13)%;P<0.01].Meanwhile,a significantly higher percentage of CD4+CD25+regulatory T ceHs and CD4+CD25high regulatory T cells in the C4+T cells population was observed in all decidua samples compared to peripheral blood both in ICP patients(P<0.01)and in control women(P<0.01).In addition,the percentages ofCD4+CD25+ regulatory T cells and CD4+CD25high regulatory T ceHs in the CD4+ T cell population in both decidua and peripheral blood in severe ICP[(15.94±1.95)%,(7.17±1.17)%,(1.87±0.90)%,(0.68±0.19)%]were lower than those in mild ICP [(18.43±1.90)%,(8.74±0.96)%,(2.62±0.72)%,(0.89±0.20)%] and normal pregnancy(all P<0.05).Conclusions CD4+CD25high regulatory T cell may play all important role in the pathogenesis of ICP and control of disease progression.

While only in minute quantities in the body, trace elements are crucial co-factors and regulators of normal metabolism.Commonly tested elements include calcium, magnesium, iron, zinc, and copper.Imbalance or deficiencies in trace elements can cause growth retardation, malnutrition, immune malfunction and other abnormalities in children.Because these elements are in trace amounts, sensitivity and reproducibility of detection methods are of paramount importance.Different methods for the same element can yield different reference intervals, which, in order to be applied in the clinic, would have to be independently established by individual laboratories.Clearly defined indications and good quality control to ensure accuracy and sensitivity are necessary for the measurement of trace elements to provide objective reference for clinical diagnosis.

Objective To investigate the clinicopathological significance of upregulated NQO 1 protein expression in uterine cervix carcinoma ( UCC) .Methods Immunohistochemical staining was performed on paraf-fin-embedded UCC specimens from 123 patients.Disease-free survival(DFS)and overall survival(OS)rates for all cervical UCC patients were calculated using the Kaplan -Meier method ,and univariate or multivariate analyses were performed using the Cox proportional hazards regression model .Results The NQO1 protein showed a main-ly cytoplasmic staining pattern in cervical cancer cells ,and the strongly positive rate of NQO 1 was significantly higher in UCC.High-level NQO1 was closely associated with poor differentiation ,late-stage,lymph node metas-tasis and high-risk for HPV infection.Additionally,high-level NQO1 was associated with lower DFS and OS rates .Furthermore ,Cox analysis revealed that NQO 1 expression emerged as a significant independent hazard factor for DFS rate in patients with UCC .Conclusion NQO1 overexpression might be an independent biomarker for prognostic evaluation of UCCs .

As a new emerging infectious virus, 2019 novel coronavirus (2019-nCoV) has been widely popular all over the world since the beginning of 2020. As a special group, children have similarities and differences with adults for coronavirus disease 2019 (COVID-19) in the epidemiology, diagnosis and treatment, curative effect monitoring, prognosis, virus testing methodology, infection and inflammation markers, etc. This paper mainly reviews the characteristics of COVID-19 in children from two aspects: the epidemiological and pathogenic detection characteristics, so as to provide some basis for its diagnosis and treatment.

OBJECTIVE:To provide references and suggestions for building and improving the R&D incentive policies for or-phan drugs in China. METHODS:The R&D incentive policies for orphan drugs in USA and the EU were compared in aspects of its legislative history,incentive measures and effects. And suggestions for improving related policies in China were put forward. RE-SULTS & CONCLUSIONS:The R&D incentive policies for orphan drugs in USA and the EU respectively started from Orphan Drug Act in USA(1983)and Orphan Drug Management Specification in the EU(1999),then formed relatively complete system by continuous improvement. The USA and the EU showed differences in its certification standard,procedure and specific incentives [R&D funding,tax deduction,fee reduction,additional incentives for micro and small and medium enterprises (SMEs),market exclusivity and special approval procedure],etc. In terms of fee reduction,for example,prescription application fee,production cost and drug confirmation fee were exempted in USA,while arrangement assist fee,initial and follow-up fee,checking fee before approval,initial listing type were reduced to a certain percentage in the EU. After developing incentive policies for orphan drugs, there is great increase in numbers of recognized qualifications and listing,SMEs have become new force in orphan drug R&D, R&D investment covers all types of diseases,orphan drug R&D are becoming the main direction of drug innovation and biotechnol-ogy development. China should determine the relevant legislation of R&D incentives for orphan drugs as soon as possible,set certi-fication and improve specific measures of R&D incentives for orphan drugs from multiple aspects,while strengthen the cooperation with other countries in qualification and R&D incentives.

OBJECTIVE To improve the knowledge of pulmonary aspergillosis. METHODS We analyzed the clinical features of 17 cases of pulmonary aspergillosis inpatients from May 1994 to Apr 2006 in our hospital retrospectively. RESULTS Sixteen cases were infected out of hospital,1 case was infected in hospital.The correct diagnostic rate of pulmonary aspergillosis was only 21.4% in the patients infected out of hospital,70.6% cases infected out of hospital were misdiagnosed to as tuberculosis or lung cancer. CONCLUSIONS The misdiagnosed reasons are that the some doctors are short of perceptual knowledge of aspergillosis and pulmonary aspergillosis confuses easily with TB and cancer and other underlying diseases.

Purpose To investigate the clinical and pathological features of inflammatory fibroid polyps ( IFPs) and to discuss its diag-nosis, differential diagnosis and treatment. Methods Clinicopathological data of 14 cases of IFPs were collected and analyzed retro-spectively. Results Most of the 14 cases were found to have submucosal polyps of the digestive tract protruding growing into the cavi-ty. Perivascular onion skinning which formed by spindle fibrocytes around vascular proliferation and eosinophilic inflammatory infiltrates were present as the characteristic feature of lesions. ABC results demonstrated that the lesions showed fibroblastic, myofibroblastic and dendritic cell signs. Conclusions IFPs are rare, benign tumors that can arise throughout the digestive tract, with unique histological changes in pathology. Surgical excision or endoscopic removal is the main treatment.

The resistance mechanism of gliomas to chemotherapy and radiotherapy is a complex network of many signaling pathways. It remains unclearhow the pathways interact with each other and how they were regulated. Recent studies have shown that DNA damage checkpoint pathway ( ATM、 ATR、 Chk1、 Chk2、 Rad17、 Radl 、Rad9、Hus1 et al. ) plays an important role in cell proliferation、genomic stability、tumorigenesis and the resistance to chemoradiotherapy of tumors. Inhibiting DNA damage checkpoint can increase tumor sensitivity to chemoradiotherapy and therefore improve the therapeutic effect. We review here the role of ATM in chemoradiotherapy resistance of gliomas and its associated mechanisms.

The relationship between medical practitioners and padtients always changes with time and this shift becomes quite striking nowadays.The characteristics and underlying reasons of this trend are expounded from such aspects as the changes in medical service system,the alteration in definitions of medical practitioners and patients, the advent of market economy.It is precondition and guarantee of handling the relationship properly to consummate relevant laws and codes,establish medical insurance institution,classify hospitals and set moral restraint for both medical practitioners and patients.

Purpose To investigate the prognostic significance of NQO1 protein expression in colorectal carcinoma ( CRC) patients. Methods 192 cases of primary CRC, 28 of colonic dysplasia, and 44 of adjacent non-tumor tissues were selected for immunohisto-chemical staining of NQO1 protein. Correlation between NQO1 overexpression and clinicopathologic characteristics, and the survival rates were calculated by the statistical methods. Results The strongly positive rate of NQO1 protein in CRC was significantly higher than that in gastric dysplasia and adjacent non-tumor tissues (P<0. 01, respectively). NQO1 high-expression rate was positively cor-related with differentiation, serosal invasion, lymph node metastasis, and clinical stage (P <0. 05, respectively). Survival curve showed that the disease-free survival and 5-year survival rates of the patients with high NQO1 expression were obviously shorter than those of patients with low NQO1 expression (P<0. 001, respectively). Further analysis showed that, high expression of NQO1 predic-ted the lower disease-free survival and 5-year survival rates in late-stage patients (P<0. 01, respectively). Importantly, NQO1 was an independent risk factor for the prognosis of CRC using Cox proportional hazards regression model ( HR: 1. 398,95%CI: 1. 011 ~1. 934, P=0. 043). Conclusions Detection of NQO1 protein expression in CRC has an important clinical significance, and it is ex-pected to become a new biomarker for CRC.