Objective　To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods　Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used　　3　　H-TaR incorporation.Results　The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion　mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.

Objective To study the effect of 99Tc-MDP on receptor activator of nuclear factor-κB ligand/osteoprotegerin (RANKL/OPG) system of collagen-induced arthritis (CIA) rat. Methods The CIA was established by subcutaneous injection with type Ⅱ collagen and complete Freud's adjuvant to rats, except the group (n=8, treated with 99Tc-MDP). The histopathological changes of joints were scored based on the extent of infiltration of inflammatory cells, cartilage destruction and bone erosion. The serum levels of RANKL/OPG were tested with ELISA, and the synovial membrane levels of RANKL/OPG were tested with Western blot.Repeated ANOVA and one way ANOVA were used for statistical analysis. Results The MTX group relative value of RANKL/OPG in serum [35 d (1.076±0.016), 42 d (1.140±0.005), 49 d (1.155±0.023)], and in synovial membrane (1.156±0.014), and the histopathological scores [synovial membrane (1.8±0.5), cartilage (1.9±0.6), bone (1.8±0.5) ] were lower than model group ( 1.269±0.025, 1.296±0.015, 1.340±0.011 ), (2.9±0.4, 2.6±0.5, 2.6±0.5), ( 1.340±0.013 )(P＜0.01 ). And all above index of 99Tc-MDP group ( 1.035±0.034,0.986±0.019, 0.991±0.020), (1.5±0.5, 1.4±0.5, 1.2±0.5), (0.098±0.026) were notably lower than the MTX treat ment group (P＜0.01). Conclusion 99Tc-MDP may retard the progression of the disease by decreasing the relative RANKL/OPG expression and it can initiate its effect earlier than MTX.

Objective To explore the clinical effect about the treatment of intrasacrospinal muscular approach and midline approach in lumbar interbody fixation with fusion for lumbar spinal stenosis.Methods Forty-two cases lumbar spinal stenosis were reviewed retrospectively in Meitan General Hospital from February 2009 to February 2014.All cases were divided into intrasacrospinal muscular approach and midline approach group(Group A,n =19) and midline approach group(Group B,n =23) according to the different surgical approach.Operation time,the amount of blood loss,post-operative drainage were compared.The VAS score of low back pain and leg pain preoperative and 6 months after operation were recorded.All cases were followed up for more than one year to evaluate fusion status.Results All cases were followed up for 13.0-20.0 months,with an average of (16.0± 1.2) months,all patients had bony fusion.Operation time,the amount of blood loss,postoperative drainage of Group A were significantly less than of Group B,the differences were significant ((95.1±4.5) min vs.(110.4±6.6) min,(75.2±5.1) ml vs.(159.8±7.2) ml,(53.6±4.3) ml vs.(86.2±14.1) ml;t =1.565,6.087,2.371;P＜0.05).VAS of back pain and leg pain at 6 months after operation of Group A showed better results than of Group B,the differences were significant((1.43±0.76) points vs.(1.39±1.00) points,(2.78± 1.23) points vs.(2.52±0.88) points;t =1.982,1.774;P＜0.05).Interbody fusion was obtained in two groups at the end of the last follow-up.Conclusion The intrasacrospinal muscular approach and midline approach for lumbar interbody fixation with fusion for lumbar spinal stenosis is better in less injury to paraspinal muscle,less blood loss and less post-operative drainage,which contributes a rapid recovery.

Objective:To study the expressions of Skp2 and P27 in the normal and tumor tissues of salivary glands,and then to evaluate their significance in the development and progression of salivary tumor.Methods:SP immunohistochemical method was used to evaluate the expression of Skp2 and P27 in the normal and tumor tissues of salivary glands. Results:The rates of Skp2 and P27 expression were neither significantly different between polymorphic adenoma and basal cell adenomas, nor significantly different between adenoid cystic carcinoma and mucoepidemoid carcinoma. The rates of Skp2 positive were significantly different between normal salivary glands and benign salivary tumor, as well as between normal salivary glands and malignant salivary tumor. The rates of Skp2 and P27 positive were significantly different between normal salivary glands and malignant salivary tumor.Conclusion:Skp2 overexpression and decreased expression of P27 might play an important role in the development and progression of salivary tumor, overexpression of Skp2 protein may lead to increase of P27 degradation.

Objective To investigate the effects of dieting and exercise intervention on lipid level in patients with hyperlipidemia.Methods Health education including dieting and exercise interventions was given to 1,800 patients with hyperlipidemia.Serum total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL-C)and low density lipoprotein (LDL-C)levels were assessed before and after interventions.Result TC, TG and LDL-C levels were decreased and HDL-C increased after interventions with statistical significance(P<0.01).Conclusions Dieting and exercise intervention can be effective in controlling lipid levels effectively.Health education should vary among individuals to achieve good effects.

A fructosamine (DMF) method for the estimation of Serum glycosylated LDL (GLDL) is reported. The glycosylated rate of LDL (K value) is equal to GLDL/LDL. It is concluded that fructosamine method for the estimation of GLDL and K value is more convenient, fast, and has a high specificity, and is not interfered by glutathione, other reducing substances with low melecular weight, and the variation of serum proteins. GLDL and K value are thus suggested to be a better indicator for the diagnosis and evaluation of treatment in diabetes mellitus than the glycosylated serum total proteins.

Objective To investigate the clinicopathologic features and prognosis of secretory carcinoma of the breast (SCB).Methods We enrolled 43 cases of secretory carcinoma of the breast out of invasive breast cancer patients according to the 2012 WHO Classification of Tumors of the Breast.Results The incidence of breast SCB was low,accounting for about 0.17％ of all breast cancer patients.The median age of onset was 49 years old.The diameter of tumor was 1.0-10.0 cm(mean size 3.2 cm).Pathology revealed large number of eosinophilic secretory material inside or outside the cancer cells positive for periodic acid-Schiff stains (PAS) and Alcian blue (AB).The positive rate of estrogen receptor (ER) was 34.9％ (15/43),progesterone receptor (PR) was 23.3％ (10/43),human epithelial growth factor receptor 2 (HER-2) was 25.6％ (11/43),S-100 was 97.7％ (42/43),and special PAS and AB diastase were tested and positive in 8 cases.The postoperative average follow-up time was 78 months,7 cases were found with distant metastasis.2 cases dying of distant metastasis.Conclusions SCB has a good prognosis despite of occasionally axillary lymph node metastasis,local recurrence or distant metastasis.

Objective　To study whether covering ice on head can abate headache produced by using nitroglycerin.Method　64 cases were collected from the patients who used nitroglycerin and were randomly divided into one contrasting group (group A) and one experimental group (group B).For the patients of group A,the state of headache produced by using nitroglycerin should be observed.For the patients of group B,covering ice on their head was adopted when they used nitroglycerin,and the state of headache should also be observed.Results　There were obvious difference between the two groups (P<0.001).Conclusion　Covering ice on head is a good method which can abate headache produced by using nitroglycerin.

Graduation project is key link in the cultivation of innovative talents for college teaching work,which is the need of the era.Thus,this paper introduced the current development of biomedical engineering at home and abroad,and the achievement of the graduation project of our university at present.Then,the necessity of the teaching reform and innovation of graduation project are analyzed.The methods for the teaching reform and innovation of graduation project are introduced including the choice of projects,the adjustment of the project schedule,reinforcing the practice of projects and improving the ability to apply knowledge,the assessment criteria of grades for the project,etc.

Objective To investigate the relation of the MR manifestations and the pathological characteristics of dissociated discherniation.Methods Characteristics of pathology and MRI of 23 cases of dissociated disc herniation were retrospectively analysed.Results Of 23 cases,the dissociated disc was low signal intensity(compared with spinal cord) on T_1WI and T_2WI with distinct margine in 7 cases,the adhere and inflammation were slight on pathology;iso-or hypo-signal intensity on T_1WI,low signal intensity in the center and slight high signal intensity at periphery on T_2WI with an distinct margine in 13 cases,while the adhere and inflammation were obvious on pathology;on T_1WI slight high signal intensity on T_1WI and iso-intensity on T_2WI in 3 cases,and fibroplasia was showed on patholgy.In the 12 cases with enhanced MRI,the lesions were slight enhancement at periphery and enhancement in the center in 7 cases,no enhancement in 3 cases,slight enhancement in 2 cases.Conclusion MRI signal characteristic can reflect pathologic changes expressly of the dissociated disc herniation.