OBJECTIVE:To provide reference for clinical rational use and management of anti-HIV drugs,and to provide ref-erence basis for occupational exposure to develop free drug policy. METHODS:Using retrospective study method,according to DDD calculation method recommended by WHO,consumption sum,DDDs,DDC and B/A of anti-HIV drugs in our center during 2011-2014 were analyzed and evaluated. RESULTS:The consumption sum of anti-HIV drugs increased firstly and decreased later, increasing from 512112 yuan in 2011 to 650854 yuan in 2012,decreasing to 496473 yuan in 2014. DDDs of anti-HIV drugs in our center during 2011-2014 decreased from 35932 in 2011,to 16363 in 2014,showing decreasing tendency. The consumption sum of nucleotide reverse transcriptase inhibitors during 2012-2014 took up the first place,and DDDs of nucleotide reverse tran-scriptase inhibitors took up the first place in 2011,2013,2014. DDDs of Zidovudine tablets took up the first place in 2013,2014, and that of Tangcao tablets took up top 3 places during 2011-2014. DDC of Tenofour disoproxil fumarate tablets,Zidovudine and la-mivudine tablets,Emtricitabine and tenofovir tablets,Lopinavir and ritonavir tablets were in high level,being more than 46 yuan. DDC of Zidovudine tablets,Stavadine tablets and Nevirapine tablets were in low level,being lower than 6 yuan. B/A of Tenofovir disoproxil fumarate tablets,Stavudine tablets and Efavirenz tablets ranged 0.5-1.5 in 4 years and B/A of Tangcao tablets was equal to 1,showing good synchronism of social and economical benefits. CONCLUSIONS:Clinical application of anti-HIV drugs in our center is rational basically,and the use of drug is basically consistent with the occupational exposure prevention. The use of TCM preparation is basically in line with the requirements of drug use in the clinic.

Objective To explore the relationship between serum reproductive hormone levels and premenopausal and postmenopausal breast cancer patients.Methods We recruited healthy women and breast cancer patients who came to our hospital from September 2007 to February 2012 for routine physical examination or surgery and divided them into premenopausal healthy group Ⅰ and breast cancer group Ⅰ ;Postmenopausal healthy group Ⅱ and breast cancer group Ⅱ,with 175 cases in each group.Reproductive hormone levels was measured in all cases.Results There were significant differences among the four groups on estradiol (E2),testosterone (T),progesterone (P),luteotropic hormone (LH),follicle-stimulating hormone (FSH),and prolactin (PRL) (F =76.459,57.224,82.932,161.047,74.801 and 15.246 respectively,P ＜ 0.05).In premenopausal cases:There were significant statistical difference on the levels of E2 [(61.5 ±32.2) ng/L vs.(74.1 ±41.6) ng/L],T [(48.1 ±22.2) μg/L vs.(80.1 ±41.8) μg/L],P [(2.9±1.6) μg/Lvs.(3.5 ±1.3) μg/L],LH [(1.3 ±0.9) U/L vs.(3.5 ±1.4) U/L],FSH [(14.8 ±8.9) U/L vs.(25.1 ±23.3) U/L],PRL [(15.8 ±6.7) μg/L vs.(39.4 ±27.4) μg/L] between the healthy Ⅰ group and the breast cancer Ⅰ group (P ＜ 0.05).In postmenopausal cases:There were significant statistical difference on the E2 [(18.8 ± 8.3) ng/L vs.(55.9±34.1) ng/L],T [(34.1 ±16.2) μg/L vs.(84.7 ±66.4) μg/L],P [(1.3 ±0.9) μg/L vs.(3.5 ±1.4) μg/L],LH [(38.1 ±33.7) U/L vs.(45.6 ± 31.2) U/L] in healthy group Ⅱand the breast cancer Ⅱ group (P ＜ 0.05).The difference was statistically significant on the E2 [(74.1 ± 41.6) ng/L vs.(55.9±34.1) ng/L],LH [(3.5 ±1.4) U/L vs.(45.6 ±31.2) U/L],FSH [(25.1 ±23.3) U/L vs.(70.5±58.2) U/L],PRL [(39.4 ±27.4) μg/L vs.(15.9 ±15.5) μg/L] between the breast group of premenopausal and postmenopausal (P ＜ 0.05).Conclusion Reproductive hormone levels has important clinical significance for the onset and development of breast cancer and provides a reference point for the treatment of menopausal and post-menopausal breast cancer.

Objectiv e To evaluate clinical application effect of the self-developed usableness and fully functional fuming-washing appliance used for limb rehabilitation.Methods 200 cases of patella fracture patients with knee joint stiffness were divided into the observation group and the control group with 100 cases in each group.The observation group used fuming-washing appliance for limb rehabilitation,the control group chose traditional clinical method.The clinical effect was compared between two groups.Results After 2 weeks,87 cases received excellent result,10 cases with good result,3 cases with fair result.The effective rate reached 97％ in the observation group,which was higher than that of the control group.Conclusions The fuming-washing appliance used for limb rehabilitation has definite clinical effect and good security,it can effectively promote the nursing quality and save rehabilitation nursing resources,meanwhile it can make use of the advantages and ef-fecfiveness of traditional Chinese medicine in the department of traumatology rehabilitation treatment.

ObjectiveTo observe the clinical efficacy of acupoint application plus modified Yu Ping Feng decoction in treating cold due to weak constitution.MethodNinety-six patients with cold due to weak constitution were divided into two groups. The control group was intervened by regular Western medicine, while the treatment group was by acupointapplication plus modified Yu Ping Feng decoction.ResultThe total effective rate was 91.7% (44/48) in the treatment group, significantly higher than 79.2%(38/48) in the control group. A one-year follow-up study found that the disease duration and relapse frequency in the treatment group were markedly shorter and less than that in the control group (P<0.05).ConclusionAcupoint application plus modified Yu Ping Feng decoction can produce a significant efficacy in treating cold due to weak constitution.

Pediatric cardiac injury is common in primary heart disease and secondary systemic diseases.The pathophysiological processes include ischemic injury or direct injury, as well as focal or diffuse myocardial inflammatory lesions.Myocardial injury markers, including myoglobin, creatine kinase isoenzyme muscle B and troponin, are first used for the diagnosis of acute coronary syndrome(ACS) in adult, and have greatly improved the efficiency of diagnosis with their sensitivity and specificity.However, due to the very different pathogenesis of myocardial injury in pediatric and adult ACS, as well as the particularity that children are in continuous growth and development, it is necessary to evaluate the value of the myocardial injury markers and their biological reference intervals in pediatrics.

Objective To analyze and evaluate the performance of the double antigen sandwich method reagents by comparing the detection results of three kinds of domestic hepatitis C virus(HCV)antibody detection kits with indirect method and sandwich method.Methods The different test methods were designed.The HCV antibody detection by using three kinds of reagents was performed on the collected positive and negative specimens and those specimens with positive reaction by the single and dual rea-gents were simultaneously performed the recombinant immunoblot assay(RIBA)and the nucleic acid detection;in addition,after the multiple proportion dilution,the quality control material with known content was parallelly detected with three kinds of reagents. All the detection results were conducted the comparison and the comprehensive analysis.Results Compared with the confirmation reagent,the false positive rates of two kinds of indirect method reagent and one kind of sandwich method reagent were 40.9%, 59.1% and 2.2% respectively.Their positive rates were 100%,but the analytical sensitivity of the sandwich method was higher than that of the indirect method by 1 ~4 times.Conclusion The sandwich ELISA reagent is significantly superior to the indirect EllSA reagent in the aspects of sensitivity and specificity.

The freshmen admission education in academic library is characterized by multiple contents and forms with gradual transformation of education position to network in information environment .The cases of freshmen ad-mission education in libraries of Beijing Normal University , Qinghua University , Shanghai Jiaotong University , and Jinan University were analyzed with its new techniques and new models summarized .The future direction of fresh-men admission education was forecasted , including establishment of standard freshmen education concept , use of more new techniques and more innovative methods .

Objective To investigate the correlation analysis of smoking on early outcomes in patients with coronary artery bypass graft surgery. Methods 489 patients undergoing CABG in our hospital from January 2010 to December 2012 were analyzed retrospectively. Subjects were divided into smoking group (n = 276), non-smoking group (n = 213). According to the condition of patients will quit smoking, smoking group was divided into two subgroups: quit-smoking group (n=81), not quit-smoking group (n=195). Results BMI, hypertension, hyperlipidemia, myocardial infarction, chronic obstructive pulmonary disease history were significantly higher than that of non-smoking group, the average age of left ventricular ejection fraction was significantly lower than that of the control group (P < 0.05). But there were no significant differences between sub groups (P > 0.05). If the COPD patients were excluded from the research, postoperative pulmonary complications, the time of mechanical ventilation of non-smoking groups was significantly lower than the smoking group; pulmonary complications , ventilation time of quit-smoking subgroups was significantly lower than not quit-smoking group (P < 0.05). Multivariate Logistic analysis showed that the period of hospitalization in patients with pulmonary complications is related with smoking status (P<0.05). Conclusions Smoking may increase the effect of pulmonary complications and postoperative mechanical ventilation period. Patients with CABG should be encouraged to stop smoking as soon as possible, so as to reduce the postoperative complications.

Objective: To reasonably allocate the hospital fixed assets, improve the use efficiency of hospital fixed assets and enhance the management level of hospital fixed assets. Methods: Literature method, observation method and expert interview are mainly adopted to launch comprehensive investigation on the current management conditions of the fixed assets of public hospitals in Quzhou from 2009 to 2011. Results: The allocation of assets is not reasonable, the utilization rate of large devices is not high and the return on assets is relatively low. Conclusion: Professional centralized management of large special equipment should be implemented, informatization management is comprehensively implemented, ABC management approach is applied to conduct classified management of fixed hospitals of the hospitals, so as to establish layered assets management accountability system.

Objective To explore of integrated traditional Chinese and Western medicine treatment for peri -menopausal dysfunctional uterine bleeding ( bleeding ) .Methods According to the digital table ,was ginen to 66 pa-tients with perimenopausal dysfunctional uterine bleeding were randomly divided into the two groups .The control group uses the conventional western medicine treatment ,the treatment group was treated by combined Western and Chinese medicine .Before and after treatment ,the endometrial thickness ,hemoglobin value of the two groups was recorded ,and the clinical efficacy of the two groups was evaluated .Results The treatment group in the treatment of uterine endom-etrial thickness,hemoglobin value and clinical effective rate was (6.69 ±1.96) mm,(97.04 ±15.48) g/L,97%, which was better than those of the control group [(8.09 ±2.44) mm,(87.13 ±20.83) g/L,85%] (t =2.575, 2.129,χ2 =9.452,all P<0.05).Conclusion The joint Chinese and Western medicine treatment for Peri-menopa-usal dysfunctional uterine bleeding and it is worthy of promotion .