Objective To explore the effect of self-made venous compressor on hemostasis after withdrawing femoral vein catheter in patients with liver failure.Methods 200 patients with liver failure undergoing femoral venous intubation were divided randomly into observation group and control group in equal number.Hemostasis was performed after withdrawing the catheter using the self-made venous compressor and bandage combined with pressure dressing in the former and latter group respectively.The two groups were compared in terms of the complication rate,time for hemostasis manipulation and time limb immobilization.Results The rate of complications in the observation group was significantly lower than that of the control group.The time for hemostasis manipulation and time for limb immobilization in the observation group were both significantly shorter than those of the control group(P<0.01). Conclusion Performance of hemostasis using self-made venous compressor may lower the rate of complication from withdrawing femoral vein catheter and shorten the time for hemostasis manipulation and the time for limb immobilization.

Objective To observe the protective effect of Panaxadiol Saponins (PDS)on rabbit heart failure model induced by acute alcohol infusion, and to explore its action mechanism of protecting myocardium. Methods 1 5 healthy rabbits were randomly divided into control group, model group and PDS group, 5 rabbits in each group.The rabbits in control group were given 0.2 g·mL-1 saline by intravenous drip at constant speed,the rabbits in model group were given 20% ethanol with same method, and the rabbits in PDS group were given 0.025 g·kg-1 PDS by intravenous injection before intravenous drip of 20% ethanol.The hemodynamic changes were observed by ventricular intubation;the levels of serum lactate dehydrogenase (LDH),creatine kinase (CK), and creatine kinase isoenzyme (CKMB)were determined by colormetric method.The level of malondialdehyde (MDA)in myocardial tissue homogenate,the activities of superoxide dismutase (T-SOD),glutathione peroxidase (GSH-Px)and catalase (CAT)were also detected.Results Compared with control group,the left ventricular end-diastolic pressure (LVEDP)of the rabbits in model group was significantly decreased at 30 min(P<0.05);the serum LDH,CK and CKMB levels were increased(P<0.05),the MDA level in myocardial tissue homogenate was increased(P<0.05),and the T-SOD,GSH-Px and CAT activities were decreased (P<0.05).Compared with model group,the LVEDP of the rabbits in PDS group was increased,the serum LDH,CK,and CKMB levels were decreased(P<0.05),the MDA level was decreased(P<0.05),and the activities of T-SOD,GSH-Px and CAT were increased(P<0.05).Conclusion PDS has protective effect on heart failure induced by acute alcohol infusion,and its mechanism may be related to the improvement of cardiac peroxidation.

Objective To evaluate the growth inhibition and apoptosis of human monocytic leukemia THP-1 cell line by using 5,8-dimethyl-2-β-hydroxyisovalerylshikonin (SK36) and explore its preliminary mechanism. Methods CCK colorimetric assay and cell counting was used to examine the growth inhibition of shikonin on THP-1 cells. The apoptosis of THP-1 cells was detected by Annexin V/PI double labeling. The activation of Caspase-3 apoptosis pathway was determined by FCM. The apoptosis and the necrosis of THP-1 cells were detected by the laser scanning confocal microscopy. Results When the THP-1 cells were treated with SK36 at 1.02 μg/ml for 24 h and 48 h, the growth inhibition was dose-dependent. The cell apoptotic rate of THP-1 cells treated with 1.02 μg/ml evaluated by FCM with Annexin V/PI double labeling staining were (40.61 ±2.13) % and (67.40±9.15) % at 24 h and 48 h after treatment, respectively, which were significantly higher than that of the control group [(16.97±0.61) %] ([ = 18.444, t = 9.528, P <0.01). SK36 could induce THP-1 cells apoptosis involving the activation of Caspase-3 (F= 323.61, P<0.01). Conclusion SK36 can induce human THP-1 cells to undergo apoptosis, and its primary mechanism was to activate the Caspase-3.

Objective To construct recombinant adenovirus Ad/MDC-VP1 and investigate its im-muno-boosting effect of the mice primed with the experimental DNA vaccine against Coxsackievirus infection. Methods The recombinant adenovirus Ad/MDC-VP1 was constructed and packaged. The Western blot analysis was used to verify the target protein. BALB/c mice were divided into four groups: Ad/MDC-VP1 group, pcDNA3/MDC-VP1 group, pcDNA3/MDC-VP1 prime-Ad/MDC-VP1 boost group and PBS group. The mice in each group were immunized intramuscularly. The titers of serum IgG and neutralizing antibody were tested by ELISA and trace neutralization assay, respectively. The lymphocytes proliferation activity and specific CTL cytotoxic activity were tested by CCK-8 assay. The mice in each group were challenged with le-thal dose of Coxsackievirus, and the assay of the serum virus titers and the observation of protection efficacy against Coxsackievirus infection were carried out. Results The recombinant adenovirus Ad/MDC-VP1 was successfully constructed and the target protein was expressed. It was observed that the titers of CVB3 VP1 specific antibody, lymphocyte stimulation index, CTL cytotoxicity activities and protection rate of the pcDNA3/MDC-VP1 prime-Ad/MDC-VP1 boost group were much higher than those of the rest groups( P < 0.05), and the titer of serum virus was lower after CVB3 challenged ( P < 0.05 ). Conclusion Both the cellular and humoral immune responses in mice could been significantly enhanced by the pcDNA3/MDC-VP1 prime-Ad/MDC-VP1 boost strategy.

Objective To compare the immune effects of Coxsackievirus B3 (CVB3) capsid protein VP1 expressed bacterially, recombinant adenovirus rAd/VP1 and recombinant plasmid pcDNA3/VP1which express VP1 protein in mice. Methods After expressed in prokaryotic cells, VP1 protein was purified. Recombinant adenovirus rAd/VP1 and recombinant plasmid pcDNA3/VP1 were amplified and extracted. Six to 8-week-old, male BALB/c mice were divided into four groups randomly. Each group contained 18 mice. The mice of pcDNA3/VP1 group or VP1 protein group were immunized intramuscularly with three injections at three weeks apart, of recombinant plasmid pcDNA3/VP1 at a dose of 100 μg/mouse or recombinant protein VP1 at a dose of 50 μg/mouse. The mice of rAd/VP1 group were immunized intramuscularly twice at two weeks interval with rAd/VP1 at a dose of 1.2 × 107 PFU. The control group was mock-immunized with 100 μl of PBS intramuscularly. Mice were bled from the retroorbital sinus plexus every two weeks after each immunization. ELISA and micro-neutralization test were used to detect levels of CVB3-specific IgG antibody and neutralizing antibody titers in the sera of immunized mice. Three weeks after the last immunization, the cytotoxic T lymphocyte(CTL) killing activity of spleen lymphocytes was detected with CCK-8 assay. Subsequently, virus titers in the sera of immunized mice were determined by the 50% cell culture infective dose( CCID50 ) assay on HeLa cell monolayers and percentage of animals surviving were observed after lethal CVB3 attack over a period of 21 days. Results The titers of specific IgG antibody and neutralizing antibody in sera of VP1 protein immunized mice were higher than other groups( P ＜0.05 ). While CTL killing activity of spleen lymphocytes of VP1 protein immunized mice was lower than mice in rAd/VP1 group( P ＜0. 05). Virus titers in sera of VP1 protein immunized mice were lower than the mice in pcDNA3/VP1 or rAd/VP1 groups ( P ＜ 0.05 ), while survival rate was significantly higher than these two groups ( P ＜ 0.05 ).Conclusion VP1 protein induced higher level of humoral immune response and acquired obvious immune protection effects in mice. The immunizing potency of VP1 protein vaccine surpassed plasmid pcDNA3/VP1or recombinant adenovirus rAd/VP1. It appeared to be a promising candidate among the three different vaccines.

Objective To invesitigate the application of contrast enhanced ultrasound in differentiating focal organizing pneumonia( FOP) and primary lung cancer . Methods The imaging data of 23 cases with FOP ( FOP group) and 75 cases with primary lung cancer ( primary lung cancer group) on conventional ultrasound and contrast enhanced ultrasound were retrospectively analyzed . The size and arrival time( AT) of the contrast agent and the enhanced pattern of the two groups were compared . ROC curve was created to determine the most accurate AT for differential diagnosis . Results There was no significant difference in the gender and the size of nodule between the two groups ( P > 0 .05) . The age of FOP group was younger than that of primary lung cancer group ( P = 0 .013) . The AT of FOP group was much earlier than that of primary lung cancer group [ ( 6 .9 ± 2 .4) s vs ( 11 .4 ± 4 .3) s , P = 0 .000] . In FOP group ,20 patients ( 87 .0％ ) showed centrifugal enhancement , 2 patients ( 8 . 7％ ) showed centripetal enhancement and 1 patient ( 4 .3％ ) showed diffuse homogeneous enhancement ,respectively .In primary lung cancer group , 12 patients ( 16 .0％ ) showed centrifugal enhancement , 58 patients ( 77 .3％ ) showed centripetal enhancement ,2 patients ( 2 .7％ ) showed diffuse homogeneous enhancement and 3 patients ( 4 .0％ ) showed diffuse heterogeneous enhancement ,respectively . There was significant difference in the enhanced pattern between the two groups ( P = 0 .000) . Meanwhile ,8 patients in FOP group ( 34 .8％ ) and 31 patients in primary lung cancer group ( 41 .3％ ) had unenhanced region in the nodule ( P = 0 .574) . ROC analysis demonstrated that AT of 8 .5 s was the best cut-off value for the differential diagnosis . When AT earlier than 8 .5 was taken as diagnostic criterion for FOP ,the diagnositc sensitivity ,specificity were 74 .7％and 82 .6％ ,respectively . Conclusions Contrast-enhanced ultrasound can provide evidence in differentiating FOP from primary lung cancer .

OBJECTIVE: To explore the effect of micro-suture technology in laryngeal surgery. METHOD: Sixty-one patients with benign laryngeal disease underwent microsurgery resection under general anesthesia, and the wound surface of the mucosa was sutured intermittently. RESULT: The postoperative healing time was shortened, the cicatrix on the vocal cord mucosa was reduced, the voice quality was improved significantly and the recurrence rate was reduced. CONCLUSION The micro-suture technology is effective in improving the voice quality and surgery effect significantly.
Adolescent ; Adult ; Aged ; Child ; Female ; Humans ; Laryngeal Diseases ; surgery ; Larynx ; surgery ; Male ; Microsurgery ; Middle Aged ; Sutures ; Vocal Cords ; surgery ; Young Adult

Humans ; Dementia, Vascular ; Proteomics ; Network Pharmacology ; Drugs, Chinese Herbal/pharmacology* ; Cognition

The national health industry standard (WS/T 610-2018), 'the reference of screening for elevated blood pressure among children and adolescents aged 7-to 18-years-old’, plays a significant role in the standardization of the blood pressure evaluation, the early detection of high blood pressure, and the early intervention of hypertension and other chronic non-communicable diseases among Chinese children and adolescents. This standard gives screening thresholds for blood pressure assessment of children and adolescents in different genders, ages, and heights. Given the complexity of applying this standard, it is error-prone and less efficient to evaluate blood pressure one by one or program this procedure. Therefore, this study provides a SPSS package based on the standard for researchers to download and use, combined with specific cases to guide the use of this package to evaluate the blood pressure of children and adolescents step by step, which could empower researchers to accurately and efficiently conduct blood pressure screening for children and adolescents in China.

Medical device going home is an inevitable trend, however, using these devices has potential safety risks. Through introducing the home use electronic medical device products and related medical device standards, this paper provides recommendations on construction of standard system for home use electronic medical devices, to improve the advancement of existing medical device standard system and guide future medical standardization work, to fully utilize standars's guiding and security role in the scientific and technological innovation, industrial development.