Objective To understand the glucose metabolic pregnant women of the abnormal situation ,to ex-plore the risk factors of abnormal glucose metabolism during pregnancy .Methods 1 823 cases of pregnant women were collected ,use case-control study , pregnancy women with abnormal glucose metabolism during the same period were selected as case group ,select the same period in the hospital expectant healthy pregnant women ascontrol group , Using single factor and multi-factor Logistic regression to analysis of risk factors of gestational abnormal glucose me-tabolism.Results Sugar metabolism abnormal incidence of pregnant women was 5.76%;there were 31 cases of dia-betes,impaired glucose tolerance in 74 cases.The two groups of subjects of age ,BMI before pregnanly ,family history of diabetes,production times were significant difference (all P<0.05).With the increase of age,the incidence of abnormal blood glucose increase , but there was no difference between the age of diabetes and impaired glucose tolerance(P>0.05).The multi-factor Logistic regression of gestational abnormal glucose metabolism showed :age (OR=3.786,P=0.001),BMI before pregnanly(OR=2.424,P=0.013)and family history of diabetes(OR=1.554,P=0.003)were risk factors.Conclusion Age,family history of diabetes and pre pregnancy BMI are the risk factors of abnormal glucose metabolism during pregnancy .

Objective To investigate the difference of C reactive protein level detected by immunity transmission turbidity and dry immunofluorescence.Methods The clinical data of 56 patients with various kinds of infectious diseases who were treated in our hospital from September 2012 to January 2014 was retrospectively analyzed.Results The levels of C reactive protein in the obser-vation group detected by immunity transmission turbidity and dry immunofluorescence were significantly lower than those in the control group (P <0.05),and the positive rate of C reactive protein was significantly higher than that in the control group(P <0.05).Conclusion The levels of C reaction protein detected by immunity transmission turbidity and dry immunofluorescence in pa-tients with a variety of acute infectious diseases show no significant difference,suggesting that immunity transmission turbidity can be used as an alternative to dry immunofluorescence.

Objective: To investigate the effects of hexamethylene bisacetamide (HMBA) on apoptosis and the expression of bcl-2 in human mucoepidermoid carcinoma cell line MEC-1. Methods:MEC-1 cells were oxposed to 2 mmol/L HMBA or 1 mg/L 5 -Flourouracil (5 -FU ) for 72 hours. A poptosis index, the expression of bcl-2 protein and bcl-1 mRNA were determined by morphologic observation, terminal dUTP nick and labeling(TUNEL) stain, immunohistochemistry, in situ hybridization and image analysis. Results: Apoptosis indexes(%) in HMBA treated. 5-Fu treated and control cells were 68.5, 23.6 and 2.3 respectevely. The expression of bcl-2 protein and bcl-2 mRNA in HMBA treated cells was higher than in 5-Fu treated or the control cells(p

Objective: To observe the curative effectiveness of laser therapy in 114 cases(120 eyes) posterior capsular opacity after intraocular lenses implantation in children.Methods: When the after-cataract appear,laser therapy should be performed as soon as possible.Under conscious condition,enable the children to cooperate maximally;short effect and mild mydriatic to dilate the pupil,a 4 mm diameter transparent zone in central was made allowing for satisfactory visual performance;Began with low energy(0.4 MJ) and increased progressively according to individual reaction,total energy was 20-30 MJ every time. Results: All the eyes' visual acuity recovered to the best level prior to posterior capsular(opacity) occurrence,corrected visual acuity 0.1-0.4 in 58 eyes,0.5-0.8 in 54 eyes,1.0 in 8 eyes.90 percent were succeed in one laser shot,no intraocular lenses damage was found in slit lamp microscope examination.Conclusion: laser can resect the posterior capsular opacity effectively,improve the visual function of the affected eye.Assisted with amblyopia treatment, laser capsulotomy play an important role in visual function reconstruction in these patients.

Objectives: To observe the efficacy of semiconducted laser photocoagulation on diabeticretinopathy(DR).　Methods: Retrospective analysis of 206 eyes from 140 patients receiving semiconducted laser photocoagulation therapy for DR.　Results:One hundred and eleven eyes were treated with PRP, 95 eyes were treated with mild-PRP. Visual acuity was increased by 1～3 lines in 82 eyes(35%), not changed in 94 eyes(46%) and decreased in 30 eyes(19%). On Fundus fluorescein angiography(FFA) 3 months later, new vessels shrinked and retinal edema recovered in most cases, but in 10 eyes, new vessels and small nonperfusional areas still could be seen after the treatment, which was treated again.　Conclusions: Timely and proper laser photocoagulation is an important treatment for DR, FFA is an essential guidance for this procedure.

Objectives:To observe the efficacy of doubling frequency laser for central retinal vein occlusion.　Methods:Twelve cases(13 eyes) of central retinal vein occlusion were treated with doubling frequency laser,4 eyes underwent panretinal photocoagulation, 9 eyes underwent regional retinal photocoagulation. The treatment varied from 1～3 times. Two cases received supplemental photocoagulation after reexamination. Fundus fluorescein angiography was performed in all the cases before and after the treatment and the results were compared.　Results:Laser spots were regular in perfusion area, pigment epithelium atrophy was mild and no fluorescein leakage was found at the border of nonperfusion area in the 9 eyes receiving regional photocoagulation treatment. Among the 4 eyes treated with panretinal photocoagulation, 2 eyes revealed neovascular atrophy at the posterior pole area; no fluorescein leakage was found and cystoid macular edema subsided. Another 2 eyes revealed neovascular regression and a little fluorescein leakage which needed supplemental photocoagulation. No neovascular increasing, perfusion area expanding or fluorescein leakage worsening were seen.　Conclusions:Laser therapy does not increase the central visual acuity but it dose help the absorption of retinal edema, hemorrhage and exudation, prevent the occurrence of proliferative vitreoretinopathy or neovascular glucoma and is protective to the present visual acuity.

Objective To investigate the effects of nuciferine on hyperlipidemia in mice and to clarify the molecular mechanism. Methods Mice were divided into three groups according to the diet: normal control group ( n=10 ) , model control group (n=10), and the intervention group (n=10). The normal control group was treated with common diet (ANI-76A feed:12. 4% fat, 68. 8% carbohydrate, 18. 8% protein). The model control group was induced with high fat diet (37. 1% fat, 42. 4% carbohydrate, 20. 5% protein). The intervention group was supplemented with 0. 5% nuciferine based on high fat diet. The mice were allowed free access to food and water for a total of 10 weeks. Several indices were analyzed in the 3 groups, including the body weight, serum lipid, lipid metabolism key enzyme, oxidative stress and metabolic pathway. Results Our results suggested that the high-fat diet-induced animal models developed obesity and dyslipidemia (P<0. 05). The body weight [(33.97±3.46) g vs. (27. 62±2. 87) g] and the serum lipid[(2. 73±0. 26) g vs. (1. 91±0. 21) g] were significantly decreased in the intervention group compared to the model control group (P<0. 05), except for the hypertriglyceridemia (P>0.05).Theactivityofhepaticlipidmetabolismkeyenzymes[(4.15±1.26) U·mL-1vs.(9.01±1.34) U·mL-1] andthe activity of hepatic lipase and lipoprotein lipase[(8.12±3.07) U·mL-1 vs.(13.48±3.75) U·mL-1] were elevated.Oxidative stress was also affected by nuciferine (P<0. 05). Mechanism study suggested that lipid synthesis genes (like SREBP-1c, FAS, SCD-1 and PPAR gamma mRNA) were up-regulated by high fat diet (P<0. 05), and the lipid oxidation metabolism genes, PPARαand CPT-1a mRNA, were down-regulated (P<0. 05), while the intervention group treatment reversed these changes (P<0. 05). Conclusion Nuciferine can improve hyperlipidemia, which might be related to the regulation of enzyme activity, oxidative stress and the changes of lipid synthesis and oxidative metabolism.

Objective To analyze the related risk factors of neonatal Streptococcus agalactiae infection and sensitivity of antibac‐terials ,in order to provide for provide evidence for the prevention and treatment of neonatal infection .Methods A total of 1 200 neonatal blood ,gastric juice ,pus specimens ,and maternal reproductive tract specimens were collected from Jan .2013 to Dec .2013 for bacterial culture and drug sensitive test .And clinical data about types of neonatal diseases ,maternal infection status ,mode of de‐livery ,medication in late pregnancy ,situation of neonatal death were retrospectively analyzed .Results A total of 80 cases of neo‐nates were infected by Streptococcus agalactiae ,,and the neonates diagnosed with septicemia ,omphalitis ,premature birth ,intrau‐terine infection and aspiration pneumonia were accounted for 8 .75% ,10 .00% ,15 .00% ,22 .50% and 43 .75% ,respectively .The positive rate of Streptococcus agalactiae infection in mother′s reproductive tract specimens was 51 .25% ,and the results of drug sensitive test were consistent with those of neonates .9 cases of cesarean section ,accounted for 11 .25% ;71 cases of natural child‐birth ,accounted for 88 .75% .In the 80 strains of Streptococcus agalactiae ,the sensitivity of vancomycin ,linezolid ,penicillin and ceftriaxone were all 100 .00% ,and resistance rates of Streptococcus agalactiae to erythromycin ,clindamycin and levofloxacin were higher ,and were 77 .50% ,57 .50% and 33 .75% respectively .Conclusion Maternal Streptococcus agalactiae carriers and mode of delivery may be risk factors for neonatal Streptococcus agalactiae infection .Obstetricians should pay attention to routine screening of Streptococcus agalactiae in perinatal pregnant women ,the laboratory should improve the efficacy in detecting Streptococcus aga‐lactiae and provide the results of antibacterials resistance of Streptococcus agalactiae immediately ,in order to provide references for clinical rational drug use .

A molecularly imprinted film ( MIF) was prepared on the surface plasmon resonance ( SPR) sensor chip for the detection of testosterone. The nanometer imprinted film was synthesized by surface grafting approach. First, the gold sensor chip was modified with an iniferter of benzyl diethyldithiocarbamate to create the “grafting from” polymeric surface. Grafting of the MIF onto the SPR chip was subsequently achieved through ultraviolet light-initiated copolymerization of methacrylic acid ( functional monomer ) and ethylene glycol dimethacrylate ( crosslinker ) in the presence of testosterone as a model template. The template molecules were then removed to form a MIF with specific recognition sites for testosterone. With this iniferter technique , self-aggregation in the reaction solution phase was avoided and grafting polymerization was confined to the exterior of the chip surface. The grafting process was implemented by in situ monitoring with SPR, which permitted the thickness of the film to be easily and strictly controlled. The chip surface modified with a testosterone-imprinted film was characterized by the methods of polarization modulation infrared reflection absorption spectroscopy ( PM-IRRAS ) and atomic force microscopy ( AFM ) . According to the results, testosterone-imprinted film modification of the SPR chip was achieved by the distribution of numerous homogeneous, nanoscale holes on the surface. The testosterone-imprinted film was applied on a SPR sensor chip for the detection of testosterone in the range of 2 . 5 í 10-16 to 2 . 5 í 10-6 mol/L in acetonitrile with the lowest determining concentration of 2. 5 í 10-16 mol/L. Adsorption experiments showed that there were two kinds of binding sites in the MIF, and the linear correlations between the changes in reflectivity and the concentration of testosterone were y=19. 69 + 1. 21x(R2=0. 9913) and y=11. 5 + 0. 45x(R2=0. 9895), respectively. Control experiments utilizing estradiol, estriol, and progesterone as analogues showed impressive selectivity and specificity for testosterone determination. The testosterone-imprinted film reproducibility was evaluated with five cycles of rinsing-rebinding and the RSD was 16 . 8% and 11 . 2% for the SPR angle changes in rinsing and rebinding respectively, demonstrating that the MIF-modified SPR sensor had good reproducibility and repeatability. Finally, the SPR sensor was successfully used to determine testosterone in artificial urine samples with recoveries from 85 . 2% to 92 . 8%.

Objective:To observe slit expression on the model of DMBA induced cheek pouch caricnoma in hamsters in various stages, so as to elucidate the relationship between slit expression and angiogenesis.Methods:Carcinogenesis model (HBPCa) was established in 60 hamsters by topical application of 5 g/L DMBA in left buccal pouch three times per week for 14 weeks.Slit expression, vascular endothelial growth factor (VEGF) and von Willebrand factor (v-WF) were examined by immunohistochemical staining,microvascular dense(MVD) was measured 6-16 weeks respectively after application of DMBA.Results:Carcinoma was developed in all the hamsters.Slit,VEGF and v-WF expression and MVD were all increased with the development of carcinoma(P