The performance and clinical application of the skull repair materials differ depending on their variety. Organic glass has ever been widely applied, but its biocompatibility is poor with the high infection rate of subcutaneous effusion; Bone cement is good at histocompatibility but bad at absorption, thus can be only used for parts of skull repair; Medical silica gel polyester grid is cheap and effective, however, local infection and material's expose occur, leading to unsatisfactory appearance; Titanium and Titanium-alloy, as one kind of high polymer materials, have achieved widely application on the clinical practice for repairing skull defects due to their good biocompatibility and physicochemical property, but many shortages are also observed. With the development of the society, patients require more and more, some insufficient materials for the repair have been abandoned, and the materials for repairing skull defects should be selected according to the individual condition. In this paper, various materials of the skull repair were compared concerning the property and clinical application, to search an optimal material for the skull repair, with the good biocompatibility and good clinical efficiency.

[Objective] To investigate the relevant factors of neonatal hemolysis, especial y the association between maternal health and neonatal hemolysis. [Methods]O and Rh positive pregnant women in late pregnancy in 497 cases, make physical identification, and track whether the newborn hemolysis. [Results] There were significant differences in different physical pregnant women in the postpartum hemolysis newborn during pregnancy, the partial repletion heat, phlegm dampness postpartum hemolytic disease of the newborn was a high incidence of pregnancy, the lowest incidence rate and quality of postpartum hemolytic disease of the newborn. [Conclusion]The constitution(partial phlegm, partial heat) pregnant women for health care, to achieve peace and reduce neonatal ABO hemolytic was significant.

Glypican-3 ( GPC3 ) plays very important role in the regulation of cell growth and differentiation in hepatocellular car-cinoma ( HCC ) . GPC3 is closely related to the occurrence and development of HCC. A dramatic elevation of GPC3 expression has been reported in a large proportion of HCC, which suggests that GPC3 is remarkably sensitive and specific to the diagnosis of HCC. GPC3 is a potential therapeutic target of HCC. This paper reviews the structure and function of GPC3, the progress of im-munotherapy based on GPC3 of HCC, and discusses the prospect of therapeutic target of liver cancer in the future.

Bioremediation is one of the most effective ways to treat and dispose of chlorinated hydrocarbons, and methanotrophs are potentially useful to do so. Recent studies found that facultative methanotrophs can use compounds containing C-C bond as sources of carbon and energy, thus overcoming the limitation that obligate methanotrophsone uses only C1 compounds for this process. This is a unique metabolic approach that is becoming increasingly attractive in the field of contaminant biodegradation. Here, we summarized the bioremediation of chlorinated hydrocarbons by obligate and facultative methanotrophs. This process involves the degradation of various chlorinated hydrocarbons by diverse strains, including pure cultures and mixed cultures. We also compare the activity expression and catalytic properties of different types of methane monooxygenases in various substrates. We furthermore summarize the kinetic characteristics of the degradation of chlorinated hydrocarbons using the model strain Methylosinus trichosporium OB3b, and outline the degradation and potential of chlorinated hydrocarbons by facultative methanotrophs. Lastly, we discuss current problems and future research directions for degradation of chlorinated hydrocarbons by methanotrophs.
Biodegradation, Environmental ; Hydrocarbons, Chlorinated ; metabolism ; Methylosinus trichosporium ; metabolism ; Oxygenases ; metabolism

Objective To observe pulmonary fibrosis in severe acute respiratory syndrome (SARS) and to discuss the mechanisms of pulmonary fibrosis in SARS. Methods Hematoxylin and Eosin staining (H&E), histology staining and immuno-histochemical staining (SP methods) were used to investigate the lungs from 4 autopsy cases. Antibodies against collagen type Ⅲ, ?-smooth muscle actin(?-SMA), Fas, FasL and transforming growth factor ?1(TGF-?1) were used for immunohistochemical studies. Results All these four lung tissues showed different degree of pulmonary fibrosis, including the organization of exudative fibrin, glomerulus-like fibrosis in alveolar spaces, the thickening of the alveolar septum, proliferation of fibroblasts, the hyperplasia of collagen fibers and the consolidation of lungs. Sirius red staining and collagen type Ⅲ staining showed the type Ⅲ and the type Ⅰ collagen fibers were the main components of the hyperplastic collagen fibers. ?-SMA were expressed in fibroblasts, immunoreactivity to Fas, FasL, TGF-?1 were all positive and located in plasma of pneumocytes, macrophages and lymphocytes. Conclusions The pulmonary fibrosis can be observed early in SARS patients and the pathogenesis may be involved in the co-effect of many effective cells, inflammatory mediators and cytokines.

OBJECTIVE:To establish a method for the determination of related substances in Pantoprazole sodium for injec-tions. METHODS:HPLC method was adopted. The determination was performed on Kromasil Hypersil ODS column with mobile phases consisting of 0.01 mol/L potassium dihydrogen phosphate buffer solution(pH adjusted to 7.0)-acetonitrile(gradient elution) at a flow rate of 1.0 mL/min. The detection wavelength was set at 290 nm,and the column temperature was 40 ℃,and injection volume was 20 μL. RESULTS:The linear ranges of impurity A,impurity B,impurity C+E,and impurity D were 0.4168-1.0420μg/mL(r=0.9998),0.1950-0.4875 μg/mL(r=0.9999),0.3890-0.9725 μg/mL(r=0.9998),0.1986-0.4965 μg/mL(r=0.9998), respectively. The limits of quantitation were 0.834,0.780,1.556,0.794 ng/mL;the limits of detection were 0.417,0.390,0.778, 0.397 ng/mL,respectively. RSD of precision test was lower than 1.0%;in repetitive test,RSD for total peak area of impurity was lower than 1.0% ;the recoveries were 98.81% -102.49%(RSD=1.18% ,n=9),95.31% -98.44%(RSD=0.91% ,n=9), 96.88%-98.44%(RSD=0.52%,n=9)and 97.87%-101.28%(RSD=1.05%,n=9). CONCLUSIONS:The method is convenient, accurate and suitable for the determination of related substance in Pantoprazole sodium for injection.

Objective To compare the effects of different composite resins as core material on the degree of microleakage in post-core repairation.Methods A total of 46 recently extracted single-rooted mandibular premolars were distributed into different groups according to different core material including ParaCore,AP-X and Ceramage.Then we used direct or indirect forming method to make post-core restorations.All of the specimens were submerged in dyes.Then,they were demineralized,dehydrated and processed to be transparent.The extent of the dye leakage was examined under a stereomicroscope.Results The microleakage value was significantly higher in Group of direct-mold-cement with ParaCore (4.94± 1.71)mm than in Group of indirect-mold-secondary-cement with ParaCore (0.91 ± 0.33) mm,Group of indirect-mold-secondary-cement with AP-X (0.87 ± 0.27) mm,and Group of indirect-mold-secondary-cement with Ceramage (1.02 ± 0.34)mm.Conclusion Different methods of building and cementing FRC post-core restorations,but not different composite resins as core material,have significant effects on the extent of microleakage in post-core repair.

Objective Observed the mobilization effects、 the safety and feasibility of autologous circulating blood stem cell by G-CSF in acute myocardial infarction (AMI). Methods 45 patients with AMI were randomly allocated to receive either inclusive type Granulocyte Colony-Stimulating Factor (G-CSF), to mobilize the stem cell. The patients received the dose of G-CSF 300?g-600?g/day, by hypodermic injection, and the duration of applying G-CSF was 5 days. In the process of the mobilization of the circulating blood stem cell, prior to applying G-CSF and on the 3rd、4th、5th、6th、7th after applying G-CSF, the white blood cell (WBC) and CD34+ cell count in the circulating blood should be observed; and the following side effects also should be paid attention to, such as: bone pain, tetter, fever, gastrointestinal effects( nausea 、vomit、 coprostatis ),deteriorated of angina or heart failure and some rare effects(spontaneous spleen rupture, severe purulent infection, hypercoagulable state, autologous immune diseases). Results Prior to applying G-CSF and the 3rd、4th、5th、6th、7th after applying G-CSF, the counts of WBC were (8.42?2.59)?10 9/L、(31.28?8.34)?10 9/L、(35.24?9.38)?10 9/L、(37.03?13.07)?10 9/L、(35.34?14.68)?10 9/L、(20.35?9.22)?10 9/L;the counts of CD34+ cell were (14.89?11.46)?10 6、(67.78?50.88)?10 6、(124.79?136.13)?10 6、(208.92?206.97)?10 6、(206.10?184.57)?10 6、(66.63?56.56)?10 6;The peak of curve that WBC and CD34 + cell count changed with applying days was on the 5th .The count of CD34 + cell in the circulating blood was positive referent with the count of WBC in the circulating blood(r=0.835);was not reference with age、 sex、body weight、and the onset time of AMI. There were total 17 complications during the mobilization of circulating blood stem cell. The incidence of complications during mobilization is 37.8%(17/45), including bong pain being 15.6%(7/45)、fever being 6.7 %(3/45)、pale being 4.4 %(2/45)、tetter being 4.4 %(2/45)、deterioration of heart failure being 4.4 %(2/45),spleen thrombosis being 2.2 %(1/45).No death happened. Conclusion : In patients with AMI, the mobilized peak of WBC and CD34 + cell counts changed with applying days was at the 5th, and the count of CD34 + cell in the circulating blood was positive referent with the count of WBC in the circulating blood(r=0.940),was negative referent with body weight of patients(r=-0.398). And mobilization of autologous circulating blood stem cell was feasible and safe.

OBJECTIVE To analyze the traits and the related factors of the injured patients who had hospitalized in ICU in order to make the corresponding strategies and decrease the incidence of hospital infection effectively. METHODS The hospital infection and the associated factors of 384 cases in ICU from Jan 2006 to Dec 2007,including the patients age,injuried severity,interventional manipulation,emergency operation before or after admission to ICU,the length of stay in ICU and the use of tranquillizer were investigated. RESULTS Of 384 cases,156 cases occurred in patients with nosocomial infection,with the infection rate 40.6%,infection site or respiratory tract was accounted for 63.3%,followed by urinary tract,skin and soft tissue.Gram-negative bacteria,were accounted for 71.7%,followed by Gram-positive cocci(15.2%) and fungi(13.1%),the severe level of diseases,interventional manipulation,emergency operation,the use of tranquillizer,the length of stay in ICU were related to the hospital infection. CONCLUSIONS The key measures to preventing hospital infection are strengthening the care of the injured patients before admission to ICU,establishing the nursing regulations of the interventional manipulation,performing sterile manipulation strictly,and enhancing the monitoring and administration about environment to control the cross-infection.

The effects of sophoramine (SA) on immune system in normal mice were studied. SA 15. 20mg/kg reduced the weight of thymus,decreased the rate of E rosette,and also suppressed T lymphocyte transformation. The weight and the hemolytic plaque of spleen were not influenced by SA at 15. 20mg/kg. At the dose of 25mg/kg,SA reduced the weight of thymus and spleen,decreased the rate of E rosette and suppressed the T lymphocyte transformation. All of the doses of SA didn' t affect the phagocytotic function of peritoneal macrophage.