1.Sealing effects of two desensitizers on the dentinal tubule:a scanning electron microscope observation
Na LI ; Lijiang ZHU ; Xianglan WANG ; Zhaojie ZHENG
Chinese Journal of Tissue Engineering Research 2015;(38):6150-6154
BACKGROUND:Both Ms Coat and Green OrTM desensitizers can seal dentinal tubules effectively, isolate the dentinal tubule from external stimuli and also reduce the dentinal hypersensitivity. The scholars around the world have discussed their effects from the descriptive and qualitative view.
OBJECTIVE:To quantitatively assess and compare the sealing effects of Ms Coat and Green OrTM desensitizers on dentine tubules.
METHODS:Thirty fresh human third molar samples were made into 3-mm dentin specimens that were treated with 17% EDTA for 2 minutes and randomly divided into three groups: Ms Coat group, Green OrTM group and control group with no treatment. In the former two groups, MS Coat desensitizer and Green OrTM desensitizer were applied respectively on the dentin surfaces. Scanning electron microscope was used to observe the dentin surface and longitudinal sections.
RESULTS AND CONCLUSION: (1) Surface observation: In the control group, al of the dentinal tubules were exposed; in the MS Coat group, it was observed that almost al the dentinal tubules were obliterated; in the Green OrTM group, the dentin tubules were covered with irregular crystaline substance and only a smal number of exposed dentine tubules were visible but narrowed obviously. (2) Longitudinal profile observation: In the control group, the dentin tubules were strip-shaped and arranged in paralel without any blockage; in the MS coat group, the tubules were covered with dense sediments, and the dentinal tubules were basicaly closed; in the Green OrTM group, there were scaly crystaline material deposited within the dentinal tubules. Dentinal diameter and number of dentinal tubules were ranked as folows: control group > Green OrTM group > MS Coat group (P < 0.05). Both Ms Coat and Green OrTM desensitizers can occlude the tubules effectively, and moreover, the MS Coat is superior to the Green OrTM.
2.Risk factors of non-alcoholic fatty liver disease in children
Zuotao GENG ; Dan ZHANG ; Xiaotong ZHU ; Lanyi LI ; Xuehai LI ; Yongli YANG
Chinese Journal of Child Health Care 2024;32(4):412-418
Non-alcoholic fatty liver disease(NAFLD) has become the main cause of chronic liver disease in children worldwide, and the incidence of NAFLD shows an increasing trend year by year. The risk factors leading to the onset of NAFLD in children are diversified and different from those in adults. At present, most medical institutions still pay little attention to NAFLD in children. This paper summarizes the risk factors and mechanisms for NAFLD in children, including gene polymorphism, maternal and fetal conditions, diet and living habits, environmental exposure, metabolic syndrome, endocrine-related mechanisms and intestinal microecology, in order to provide reference for the prevention and management of childhood NAFLD.
3.Determination of the Early Time of Death by Computerized Image Analysis of DNA Degradation: Which Is the Best Quantitative Indicator of DNA Degradation?
Lijiang LIU ; Xiji SHU ; Liang REN ; Hongyan ZHOU ; Yan LI ; Wei LIU ; Cheng ZHU ; Liang LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(4):362-366
This study evaluated the correlation between DNA degradation of the splenic lymphocytes and the early time of death, examined the early time of death by computerized image analysis technique (CIAT) and identified the best parameter that quantitatively reflects the DNA degradation.The spleen tissues from 34 SD rats were collected, subjected to cell smearing every 2 h within the first 36 h after death, stained by Feulgen-Van's staining, three indices reflecting DNA content in splenic lymphocytes, including integral optical density (IOD), average optical density (AOD), average gray scale (AG) were measured by the image analysis. Our results showed that IOD and AOD decreased and AG increased over time within the first 36 h. A stepwise linear regression analysis showed that only AG was fitted. A correlation between the postmortem interval (PMI) and AG was identified and the corresponding regression equation was obtained. Our study suggests that CIAT is a useful and promising tool for the estimation of early PMI with good objectivity and reproducibility,and AG is a more effective and better quantitative indicator for the estimation of PMI within the first 36 h after death in rats.
4.The expression of serum miRNA-24 and miRNA-509 in patients with hepatocellular carcinoma and their correlation with prognosis
Wu PANG ; Lijiang CHEN ; Yu ZHU ; Yakun WU ; Wei ZHANG
Practical Oncology Journal 2023;37(5):411-415
Objective The objective of this study was to investigate the expression and clinical significance of serum miR-NA-24(miR-24)and miRNA-509(miR-509)in patients with hepatocellular carcinoma(HCC).Methods Ninety-four HCC patients(HCC group)who visited Suining Central Hospital in Sichuan province from January 2019 to October 2020 were select-ed,and 90 healthy subjects(control group)who underwent the physical examination center at the same time were selected.The ex-pression of miR-24 and miR-509 in human liver cancer cell lines and the serum of participants in the two groups was detected by real-time quantitative polymerase chain reaction(qRT-PCR).The correlation between miR-24 and miR-509 levels and the clinicopathological characteristics and prognosis of HCC was analyzed.Results The expression of miR-24 in the serum of HCC pa-tients was significantly higher than that of the control group(3.19±0.29vs.0.66±0.20),(t=-68.601,P<0.01),and the ex-pression of miR-509 was significantly lower than that of the control group(0.74±0.27 vs.1.24±0.28),(t=12.331,P<0.01).The expression of miR-24 in serum was positively correlated with alpha fetoprotein(AFP)level,metastasis,and TNM stage(r=0.821,0.510,0.762,P<0.01),and negatively correlated with the degree of differentiation(r=-0.771,P<0.01).The expression of miR-509 in serum was negatively correlated with AFP level,metastasis and TNM stage(r=-0.820,-0.506,-0.766,P<0.01),and negatively correlated with the degree of differentiation(r=0.775,P<0.01).Conclusion The expression of serum mir-24 is up-regulated in HCC patients,while the expression of serum mir-509 is down-regulated.Both of them are closely related to HCC metastasis and malignancy.Clinical testing of serum miR-24 and miR-509 levels in patients can help diagnose and evaluate the condition of HCC.
5.DNA barcoding of Gentiana crassicaulis in Lijiang and evaluation of root processing methods
Wen-jing JI ; Yu-xuan ZHANG ; Zhi-li ZHAO ; Liang-hong NI ; Wei-tao LI ; Chen-xin ZHU ; Xiang CHEN ; Shao-hua YANG
Acta Pharmaceutica Sinica 2022;57(2):507-513
The key factors for producing the best quality Chinese herbal medicines are high-quality germplasm, suitable cultivation area and the proper processing methods for herbal raw materials.
6.Antibiotics-mediated intestinal microbiome perturbation aggravates tacrolimus-induced glucose disorders in mice.
Yuqiu HAN ; Xiangyang JIANG ; Qi LING ; Li WU ; Pin WU ; Ruiqi TANG ; Xiaowei XU ; Meifang YANG ; Lijiang ZHANG ; Weiwei ZHU ; Baohong WANG ; Lanjuan LI
Frontiers of Medicine 2019;13(4):471-481
Both immunosuppressants and antibiotics (ABX) are indispensable for transplant patients. However, the former increases the risk of new-onset diabetes, whereas the latter impacts intestinal microbiota (IM). It is still unclear whether and how the interaction between immunosuppressants and ABX alters the IM and thus leads to glucose metabolism disorders. This study examined the alterations of glucose and lipid metabolism and IM in mice exposed to tacrolimus (TAC) with or without ABX. We found that ABX further aggravated TAC-induced glucose tolerance and increased insulin secretion. Combined treatment resulted in exacerbated lipid accumulation in the liver. TAC-altered microbial community was further amplified by ABX administration, as characterized by reductions in phylum Firmicutes, family Lachnospiraceae, and genus Coprococcus. Analyses based on the metagenomic profiles revealed that ABX augmented the effect of TAC on microbial metabolic function mostly related to lipid metabolism. The altered components of gut microbiome and predicted microbial functional profiles showed significant correlation with hepatic lipid accumulation and glucose disorders. In conclusion, ABX aggravated the effect of TAC on the microbiome and its metabolic capacities, which might contribute to hepatic lipid accumulation and glucose disorders. These findings suggest that the ABX-altered microbiome can amplify the diabetogenic effect of TAC and could be a novel therapeutic target for patients.