Invasion and migration are distinctive features of the malignant tumors.Studies showed that epithelial-mesenchymal transition ( EMT) , a conversion process with the loss of epithelial cell features and the gain of mesenchymal phenotype has been recog-nized as a key element of invasion and migration of malignancy.When EMT occurs, the downregulation of E-cadherins and the loss of adhesion in extracellular matrix play a critical role which are regarded as important indicators in the assessment of EMT.The latest re-searches indicated that integrin, one of the cell adhesion molecules family, was involved in EMT directly or indirectly through mediating either adhesion among cells, extracellular matrix or signal pathways by activating multiple kinases tyrosine phosphorylation cascade. The relationship between EMT and integrin is summarized in this article.

Long non-coding RNAs ( LncRNAs) are a class of RNA transcripts greater than 200 nt in length without the func-tion of protein encoding.LncRNAs, which consist of a great variety of categories and structures, regulate the associated gene expression on multiple levels, including chromatin modification, transcriptional and post-transcriptional regulation, and epigenetic modification. Moreover, LncRNAs are widely involved in the physiological and pathological processes.Recently, a large number of studies have found that the abnormal expression of LncRNAs is closely related to the occurrence, development, invasion and metastasis of tumor, and plays an essential role in pathogensis of colorectal cancer.In this paper, the function of LncRNAs in colorectal cancer is reviewed, which provides a theoretical evidence for its use in the diagnosis and treatment of colorectal cancer in clinical study.

Objective To explore the feasibility and proper time of laparoscopic cholecystectomy(LC)for the treatment of gallstones complicated with acute biliary pancreatitis(ABP).Methods From January 1999 to August 2007,72 patients with gallstones complicated with ABP were treated with LC in our department.Among the patients,early LC was performed on 56 cases when their symptoms of pancreatitis were relieved,elective LC was carried out in 8 patients,while emergent LC was required in the other 8 patients because of failure of conservative treatment.Results The LC was successfully completed in all the cases with a mean operation time of 63 min(30-135 min),and a mean blood loss of 50 ml(20-230 ml).Intraoperative cholangiography was carried out in 5 patients,4 of them were diagnosed as having stones in the lower common bile duct,and were then cured by endoscopic retrograde cholangiopancreatography(ERCP)and endoscopic sphincterotomy(EST)after LC.Only one patient was converted to open surgery.No perioperative death,or postoperative biliary hemorrhage or leakage occurred in this series.After the operation,pathological examinations revealed multiple small or sand-like stones in the resected gallbladders.The patients were followed up for 2 months to 8 years(mean 19 months);during this period,no recurrent pancreatitis or cholelithiasis was found.Conclusions LC is feasible and safe for patients with gallstones complicated with acute pancreatitis at early stage if perioperative treatments has been properly carried out.Preoperative MRCP,intraoperative cholangiography,and postoperative EST are essential auxiliary techniques for the treatment.

Objective To evaluate the role of bispectral index (BIS) in assessing the depth of sedation in hypogastric operation of children. Methods Forty children undergoing hypogastric operation were divided into two groups with 20 cases each by pre-school (age 3-5) (Ⅰ group) and school (age 6-12)(Ⅱ group). Sedation was solely maintained with TCI propofol. The infusion was adjusted till adequate scdation (OAA/S≤ 1 score) before sacral block. The course of propofol infusion and BIS of recovery period were closely observed. Sedation scores were assessed by special anesthetists. Results During recovery period, the mean values of BIS were gradually increased, when BIS > 76 predicted 94% sensitivity and 50% specificity in Ⅰ group, 100% sensitivity and 100% specificity in Ⅱ group. ROC area under the curve of the two groups were 0.917 ± 0.007, 0.955 ± 0.004. Conclusions BIS can predict fairly well the level of consciences during recovering periods. BIS monitoring is an effective and reliable method to guide children recovery.

On the basis of systematicly reviewing the developmental history of acupuncture and moxibustion and profoundly understanding its academic connotations,the authors of the present article make a summary and analysis on the key factors influencing the development of acupuncturology. These key factors are 1) the emergence of "microacupuncture needle regulating-Qi" and the establishement of their corresponding theory system,2) a large number of practitioners who inherited the learnings of acupuncturology generations by generations,and abundant medical classical works which recorded the valuable academic thoughts and clinical experience of the predecesors,3) the application of acupuncture charts and manikins,and 4) modernizing changes of acupuncture learnings after introduction of western medicine to China. Just under the influence of these key factors,the acupuncture medicine separates itself from the level of the simple experience medicine,and has formed a set of special theory system and developed into a mature subject.

Objective To understand the situation of college students' awareness about innovative credit, analyze the influencing factors, in order to provide a scientific basis for constructing innovation credits and promoting the double strategy. Method The undergraduates of a medical university who were still at school in April 2015 were selected, and by using a stratified cluster sampling method, 450 students were chosen to be conducted by self-administered questionnaire survey to understand the cognitive status of in-novation of credit system in college students. Chi square test was used to compare the passing rates of students in different demographic characteristics, and the relationship between scores was analyzed by linear regression analysis. Result A total of 439 valid questionnaires were collected , and the total scores of innovative knowledge, attitudes and behaviors of 439 undergraduates were 15.95％ (70/439), 36.44％ (160/439) and 32.80％(144/439) respectively. In the part of knowledge innovation credits, girls' passing rate was higher than boys ( χ2=4.010,P=0.045. In the part of attitude, high grade group students had more positive attitude than the low grade group of students ( χ2=6.227,P=0.0013). In the part of behavior; higher grade students with innovative credits had higher pass rates than those in lower grades (chi, 2=7.781, P=0.005), and boys had a higher rate of passing than girls ( χ2=6.658,P=0.010). The total score of knowledge was positively linear with the total score of attitude and behavior. Conclusion College Students' awareness of innovation credit rate is low, but the innovative attitude and behavior is positive. The higher the awareness rate is conducive to the cultivation of attitude and behavior, so it is necessary in medical colleges to carry out innovation of credit and promote the cultivation of innovative consciousness.

This study was aimed to explore the medication rules of Chinese herbal medicine compound in the treatment of primary dysmenorrheal based on data mining.Relevant literatures on Chinese herbal medicine compound in the treatment of primary dysmenorrhea were retrieved from June 2006 to June 2016 in the Chinese Journal Full-text Database (CNKI) to establish a literature database.The Excel 2010,SPSS 22.0 and SPSS Modeler 14.2 software were used in the frequency analysis,cluster analysis,association analysis,and etc.The results showed that literatures related to Chinese herbal medicine compound in the treatment of primary dysmenorrhea,there were 74 compound formula and 76 kinds of Chinese herbal medicine involved.Angelica was the core single Chinese herbal medicine.Chinese herbal medicine with high-frequency usage was the type of removing blood stasis,invigorating qi and blood.Cluster analysis can form 5 clustering combination.Primary dysmenorrhea was mainly clustered to two syndromes,which were qi and blood deficiency with blood stasis,liver and kidney deficiency with qi stagnation and blood stasis.The association rules analysis showed that there were a total of 25 rules,with the most common combination of two or multiple combination from four kinds of Chinese herbal medicine,including Angelica,Radix Paeoniae Alba,Rhizoma Chuanxiong,and Rhizoma Corydalis.It was concluded that blood stasis was the direct cause of primary dysmenorrhea,many of which belonged to the deficiency of root (i.e.,deficiency of qi,blood and cold deficiency) and excess of branch.Chinese herbal medicine treatment should be focused on the regulation of both qi and blood,invigoration of both liver and kidney,as well as harmonization of the blood.

Aim To investigate the role of adrenomedullin on cell proliferation in renal interstitial fibrosis mice. Method Renal interstitial fibrosis model was induced with unilateral ureteral obstruction (UUO) and valsartan(10 mg?kg?d-1) was given by gavage. Immunohistochemistry and RT-PCR were used to detect expressions of PCNA, Col Ⅲ and TGF-?_1 in the kidney of wild type (WT) and adrenomedullin knockout (AMKO) +/-mice. Results The number of PCNA positive cells was increased in WT-UUO group compared with WT sham group and was partially reduced by Valsartan in WT-UUOV group. AMKO group showed higher expression of PCNA than WT group, and it increased more remarkably in AMKO-UUO mice but was reduced by Valsaltan in AMKO-UUOV group. Expressions of Col Ⅲ andTGF-?_1 were upregulated in AMKO-UUO mice, and this change was inhibited by Valsaltan.Conclusion Adrenomedullin play an important role in the process of cell proliferation in renal interstitial pathological changes by UUO.

This study was aimed to summarize and analyze acupoint selection rule of acupuncture in the treatment of primary dysmenorrhea in recent years,and to guide acupoint selection and compatibility in clinical practice.Relevant literatures in recent years were retrieved from the China National Knowledge Infrastructure (CNKI) full-text database.And then,literatures involving acupuncture prescriptions were summarized.Data were integrated and input.Finally,the SPSS software was combined to analyze the data of acupoint selection in the acupuncture prescription to achieve the conclusion.The results showed that in the acupuncture treatment of primary dysmenorrhea,the main meridian selections were the Ren Meridian and the Spleen Meridian of Foot Taiyin.The generally used acupoints were SP6-Sanyinjiao,RN4-Guanyuan,SPS-Diji,and etc.The multi-point compatibility therapy was used.It was concluded that although the advantages of acupuncture in the treatment of primary dysmenorrhea are prominent,the pathogenesis is mainly related to the liver,spleen and kidney,which are recognized by most physicians.However,there are still large differences in acupoint compatibility and acupoint selection.Hence,there are still no unified,scientific and standardized diagnosis and treatment standards.Further exploration should be made to guide clinical practice.

AIM:To investigate the effect of Shenci capsule combined with cisplatin ( DDP) in reversing DDP resistance by PI3K/AKT/mTOR signaling pathway in nude mice bearing A 549/DDP tumor.METHODS:The patient-de-rived lung adenocarcinoma A 549/DDP cell xenograft model was established .The tumor-bearing nude mice were randomly divided into control group , Shenci capsule group , DDP group and Shenci capsule combined with DDP group .The mice in control group was treated with normal saline , while the mice in other groups were treated with different drugs for 21 d.After treatment, the mice were killed and lung cancer tissues were collected .Flow cytometry was used to analyze the cell cycle and apoptosis.FQ-PCR was used to determined the mRNA levels of PTEN , P-glycoprotein, PI3K, AKT and mTOR in A549/DDP lung tumor .RESULTS:Compared with control group , the cell proliferation in all the drug treatment groups was inhibited .Compared with other drug treatment groups , Shenci capsule combined with DDP blocked the cell cycle of A 549/DDP cells at G2/M phase, promoted the apoptosis rate , increased the mRNA expression of PTEN and inhibited the mRNA expression of P-glycoprotein, PI3K, AKT and mTOR.CONCLUSION:Shenci capsule increases the sensitivity of A 549/DDP resistant cells in nude mice to DDP by blocking PI 3K/AKT/mTOR signaling pathway , increasing the expression of PTEN or inhibiting P-glycoprotein-mediated resistance pathway .