We discuss the common complications and risk of rehabilitation method tor patients with common neoplasms. The matters that should be noticed in exercise therapy was considered we also investigated inteventions for these complications.

Histopathological examination of testes is important in assessing spermatogenesis and testicular function. Modified Davidson's fluid (mDF) has been proposed as a superior substitute for Bouin's fluid (BF) for fixation of adult animal testes. Besides, 4% paraformaldehyde (PFA) has been commonly used to fix testes with convenience. We compared the morphology of the rat testis fixed in 4% PFA, mDF, or BF using hematoxylin and eosin (HE)-stained sections. Fixation in 4% PFA resulted in obvious tissue shrinkage artifacts, especially between seminiferous epithelium cells. Shrinkage artifacts were also observed in the central area of the testes fixed in BF. Use of mDF did not cause shrinkage artifacts between seminiferous tubules, though a small amount can be observed in seminiferous tubules between germ cells. Clarity of nuclear detail in testes fixed in mDF and BF is better compared to 4% PFA. Our study demonstrated that fixation in mDF provided better morphologic details in the rat testis as compared with 4% PFA and BF.

Objective: To understand and grasp the current situation and actual demands of China’s pharmaceutical industry, in order to provide a basis for the establishment of the chemometrics guideline in the Chinese Pharmacopoeia.
Methods: Online questionnaire was adopted to investigate the chemometrics backgrounds and demands of pharmaceutical industry practitioners.
Results: The practitioners of China’s pharmaceutical industry had certain expectations and demands for the chemometrics guideline, but the situation of talent reserves was not that optimistic.
Conclusions: It is extremely urgent to develop the chemometrics guideline, as general chapters of the Chinese Pharmacopoeia, which serve as the legal basis to guide the data quality control, the establishment of analytical methods and the verification of analytical methods in analytical practice, so as to ensure the scientific of multivariate analysis methods and reliability of analytical results, and will be conducive to promoting the improvement of China’s pharmaceutical level.

Objective To investigate the role of phosphatidylinositol 3-kinase (PI3K)/protein-serine-threonine kinases(Akt) signal transduction pathway in EphB receptor-mediated neuropathic pain in rats.Methods Forty-eight pathogen-free male SD rats aged 2-3 months weighing 150-180 g were randomly divided into 6 groups (n =8 each):groups sham operation (groups S1 and S2 ); groups chronic constrictive injury (CCI) (groups C1and C2 ) and groups EphBI-Fc (EphB receptor antagonist) + CCI (groups E1 and E2 ).Neuropathic pain was induced by placing 4 ligatures on left sciatic nerve at 1 mm intervals with 5-0 silk thread in groups C1,C2,E1 and E2.EphBI-Fc 0.5 μg in 5 μl normal saline was injected intrathecally 1 h before operation and at 1 and 2 d after operation (group E1 ) or on 5th day after operation (group E2).Normal saline 5 μl was injected intrathecally instead of EphBI-Fc 1 h before operation and at 1 and 2 days after operation (groups S1 and C1 ) or on 5th day after operation (groups S2 and C2 ).Pain withdrawal latency to noxious thermal stimulation (PWL) and pain withdrawal threshold to noxious mechanical stimulation (PWT) were measured before operation and at 1,3 and 5 d after operation.The animals were sacrificed at 5 d after operation after measurement of pain threshold.The lumbar segment of spinal cord (L4-6) was removed for determination of c-Fos,PI3K and phosphorylated Akt(p-Akt) expression.Results CCI significantly reduced PWL and PWT and up-regulated spinal c-Fos,PI3K and p-Akt expression in groups C1 and C2 as compared with groups S1 and S2.EphB1-Fc significantly decreased hyperalgesia and the upregulated spinal Fos,PI3K and p-Akt protein expression induced by CCI in groups E1 and E2 as compared with groups C1 and C2.Conclusion Spinal EphB receptor is involved in the development and maintenance of neuropathic pain through PI3K/Akt signal transduction pathway.

Objective To evaluate the application of fluorescence PCR in detecting ureA gene of Helicobacter pylori(HP)in feces.Methods Fluorescence PCR was used to detect ureA gene of HP in feces from 50 patients,including 23 confirmed by gastric biopsy urease test and histological staining.Bacterium culture and serum antibody detection were also performed, and chi-square test was used to compare the sensitivity,specificity,positive predictive value and negative predictive value among three methods.Results The sensitivity,specificity,positive predictive value and negative predictive value of fluorescence PCR were 1.00,0.96,96.O%and 100.0%,while those for HP culture were 0.78,1.00,100.0%and 84.0%,and thee for serum antibody detection Was 0.96,0.74,76.O%and 95.0%.There were significant differences in sensitivity and negative predictive value between PCR and bacterium culture (X2=5.60 and 4.44,P<0.05),and significant differences in specificity and positive predictive value between PCR and serum antibody detection(X2=5.28 and 4.08,P<0.05).Conclusion ureA gene detection in feces by fluorescence PCR is of value for the diagnosis of HP infection.

Objectives: Hypertensive disorders complicating pregnancy(HDCP) has a long-term deleterious effect.This study aimed to investigate the changes of cognitive function in women with HDCP and explore their possible mechanism.Methods: Fifty-three patients with HDCP and 22 normal pregnant women were included in this study,their cognitive functions assessed 60-80 days after delivery by word learning and delayed recall test,symbol digit substitution test,animal category fluency test,block design,trail making test(Part A) and digit span test.The results of the cognitive tests were compared between the HDCP and normal control groups.Results: Cognitive performances of the severe preeclampsia patients differed significantly from those of the normal individuals on the tests of word learning(P

ObjectiveTo sum up the experience in the treatment of acute arterial ischemia in the extremities. Methods From 1980 to 2001,148 patients with acute arterial ischemia in the extremities were treated by multiple-means such as: embolectomy, interventional treatment, thrombolytic and antiagglutinatives. Results The cure rate in patients treated within 12 hours was 95.5%,mortality was 4.5%,while the cure rate, alleviative rate, amputation rate and mortality in patients treated 12～24 hours after onset were 64.8%,17.6%,9.9%,7.7%,respectively and that were 20%,34.3%,25.7%,20% respectively when treatment started 24 hours after the onset. The cure rate in 19 patients treated by nonoperative means was 10.5%, alleviative rate was 73.3%, amputation rate was 15.8%. Conclusion Patients with acute arterial ischemia suffer a high mortality. Mortality and disability rate can be reduced by early diagnosis, appropriate treatment and effective management for the systemic diseases.

Objective To explore the changes in pulmonary function of patients with leukemia before and after stem cells transplantation(SCT).Methods Twenty-three patients with leukemia were assigned to group A,while 35 healthy controls to group C.All spirometric values were recorded including percentage predicted forced vital capacity(FVC),residual volume(RV),total lung capacity(TLC),forced expiratory volume in one second(FEV1),maximal expiratory flow in 50% and 75% vital capacity(MEF50%,MEF75%),diffusing capacity of the lung for carbon monoxide(DLCO),and ratio of carbon monoxide diffusion capacity to alveolar ventilation(DLCO/VA).Twenty patients with leukemia undergone stem cell transplantation were assigned to group B.Changes in lung function 7 days before and 3-12 months after transplantation were recorded.Group B was sub-grouped according to the source of stem cells and extent of anemia.Changes in lung function and infection were observed subsequently.Results Patients in group A experienced a significant decrease in predicted percentage of DLCO,DLCO/VA and MEF75%(76.25%?22.48%,83.77%?21.32% and 61.13%?19.08%,respectively),which were significantly different compared with that in group C(118.21%?19.34%,112.4%?20.96% and 81.02%?12.10%,P

Objective To investigate the expression of SOX2,TGF-β1 and Smad3 in gastric cancer and their relationships with clinical pathological characteristics of gastric cancer. Methods Immunohistochemical staining was used to detect the expression of SOX2, TGF-β1 and Smad3 expression and the clinical pathological characteristics of gastric cancer were analyzed. Results Integrated optical density (IOD) values from the positive expression of SOX2, Smad3 in gastric cancer group were all significantly lower than normal gastric tissues (P < 0.05). IOD values from the positive expression of TGF-β1 in gastric cancer group were all significantly higher than normal gastric tissues (P < 0.05). IOD values from the positive expression of TGF-β1 in lower poorly differentiated gastric cancer tissues, with lymph node metastasis and Ⅲ+Ⅳ were all significantly higher (P < 0.05). Higher IOD values from expression of SOX2 and Smad3 were found in high-moderated differentiated gastric cancer tissues, without lymph node metastasis and Ⅰ+Ⅱgastric cancer (P < 0.05). SOX2, TGF-β1 and Smad3 in gastric cancer has nothing to do with age illness and sex (P >0.05). There was positive correlation between SOX2 and Smad3 expression in gastric cancer tissues, and negative correlation between TGF-β1 and Smad3 in gastric cancer. Conclusions Abnormal expression of SOX2, TGF-β1 and Smad3 protein is related to the onset, development and clinicopathologic features of gastric cancer.

BACKGROUND:Compared with conventional medications, drug micro-capsule system can control the release of drugs and have wel target properties and biocompatibility. The drugs can be concentrated at the focus and play an important role in clinic. OBJECTIVE:To prepare dacarbazine magnetic micro-capsules with different capsule materials and gelatin complex by coacervation, and to optimize capsule materials and preparation process. METHODS:Fe 3 O 4 RESULTS AND CONCLUSION:The solution complex coacervation method was better than the emulsion coacervation method. As for the solution complex coacervation method, the optimal capsule material was gelatin-sodium alginate, with drug embedding rate 37.90%, the yield rate 72.31%, and the average magnetization intensity 8.53 emu/g. The second material was gelatin-chitosan. As a capsule material, the gelatin was better than chitosan with single coagulation method. Drug embedding rate was 51.58%, the yield rate was 64.50%, and the average magnetization was 6.93 emu/g. Single coagulation method was better than coacervation method. complex coacervation, we prepared the gelatin-Arabic gum magnetic micro-capsule, gelatin-sodium alginate magnetic micro-capsules, gelatin-sodium carboxymethyl cel ulose magnetic micro-capsules, and gelatin-chitosan magnetic micro-capsules. With the emulsion complex coacervation method, we further prepared the gelatin-Arabic gum magnetic micro-capsule, gelatin-sodium alginate magnetic micro-capsules, gelatin-sodium carboxymethyl cel ulose magnetic micro-capsules, and gelatin-chitosan magnetic micro-capsules. The magnetic gelatin micro-capsules and magnetic chitosan micro-capsules were prepared with single coagulation method. The micro-capsules were determined for the embedding rate, the magnetic susceptibility, the micro-capsule size and the release performance, to define the optimal preparation technology of dacarbazine magnetic micro-capsules.