OBJECTIVE:To prepare jumazhitong film and establish its quality control method.METHODS:The jumazhitong film former was made with PVOH-124 as the base,the content of the principal agent-dyclonine hydrochloride was determined by HPLC.RESULTS:Good linear relation was achieved when the detection concentration range of dyclonine hydrochloride was 40.8～408?g/ml(r=0.9 998),the average recovery was 101.3%(RSD=1.3%,n=6).CONCLUSION:The preparing technique of jumazhitong film former is simple,its quality is stable and the control method is feasible.

Objective To explore the value of digital mammography in diagnosis of the negative palpable breast cancer in rural ar-eas.Methods 6 754 women randomly selected among 13 700 female outpatients (range,30-83 years)in rural areas underwent dig-ital mammography no matter they had breast palpable masses or not.Results 72 cases with breast cancer were diagnosed by digital mammography (72/6 754).Clinical palpation showed positive in 59 cases (59/72)and negative in 13 cases (13/72)with aged 35-77 years.Among 13 cases with negative palpable breast cancer,6 cases were carcinomas in situ,3 cases were intraductal carcinomas,and 4 cases were infiltrating ductal carcinomas.Conclusion Digital mammography may help in detection of the negative palpable breast cancer in early time.

Objective:To evaluate the reliability of flexural strength and subcritical crack growth(SCG)of WL and HT zirconia ce-ramics under cyclic loading.Methods:Bar-shaped speciments of WL(n =30)and HT(n =30)were fabricated and loaded in three-point bending and cyclically fatigued.The flexural strength and the number of cycles to fracture were obtained from three-point flexural test and cyclic fatigue test respectively.Data were examined using Weibull statistics.Pairs law was used to analysize the subcritical crack growth(SCG),the behavior of which was described in terms of crack velocity as a function of maximum stress intensity factor (KImax ).Results:The Weibull module(m)of WL and HT was 10.64 and 12.04,the stress corrosion cracking susceptive indexes(n) 23.71 and 32.87,the SCG 90% and 76.7%,the KImax 49.6% and 50.9% of its maximum stress intensity factor(KIC ),respectively. Conclusion:WL and HT produce pronounced SCG.The minimum fatigue crack growth rate of HT is lower than that of WL(νHT <νWL ).

OBJECTIVE: To evaluate the feasibility of a rapid genetic diagnosis technique in molecular genetics study of hearing loss. METHOD: One hundred and ten subjects including 54 with moderate to profound non-syndromic sensorineural hearing loss were enrolled in this study. All with informed consents. Their peripheral blood samples were taken and tested using Hearing Loss Array Kit developed by Capital Bio. Nine hot mutation sites from 4 high risk genes were tested each. RESULT: The overall positive rate in this cohort was 50.9%, while the positive rate among 54 hearing loss cases was 59.3%. DNA sequence analysis showed 100% coincident with gene chip results. CONCLUSION This Hearing Loss Array Kit (gene chip) has a high positive detection ratio, its characteristics such as rapid high-flux sensitive and high specific make it possible and promising in future screening and diagnosis in patients with sensorineural hearing loss. Molecular genetic diagnosis also has a profound value in deafness prevention.
Child, Preschool ; Female ; Hearing Loss, Sensorineural ; diagnosis ; genetics ; Humans ; Infant ; Male ; Molecular Diagnostic Techniques ; methods ; Mutation ; Oligonucleotide Array Sequence Analysis ; Sequence Analysis, DNA

OBJECTIVETo evaluate the F(-) releasing and recharging properties of six dental restorative materials.

METHODSDisc specimens 10 mm in diameter and 1 mm in thickness were prepared from 6 different dental restorative materials: Fuji VII [glass ionomer cement (GIC)], Fuji II LC (light-curing, GIC), Beautifil (giomer), Compoglass F (compomer), Charisma (composite) and Experimental I (a fluoride releasing composite), with 10 discs for each material. Specimens were dipped in 5 ml deionized water and F(-) release was detected using a fluoride-specific ion electrode every day from 1-7 days and every three days from 8-28 days. On day 28, specimens were exposed to a fluoride foam for 4 min and then dipped in water, then the F(-) concentration was detected every day for a week. The specimens were exposed to fluoride foam and fluoride release was detected repeatedly for three times.

RESULTSAll materials presented the highest F(-) release on the first day after dipping and the F(-) release sharply decreased after 24 h and slowly decreased after 3 days. On the first day, Fuji VII presented the highest F(-) release[(99.68±15.21) µg×cm(-2)×d(-1)], followed by Fuji II LC [(37.12±1.67) µg×cm(-2)×d(-1)], Experimental I [(22.93±1.53) µg×cm(-2)×d(-1)], Compoglass F[(15.28±0.70) µg×cm(-2)×d(-1)], Beautifil[(2.40±0.52) µg×cm(-2)×d(-1)] and Charisma[(0.11±0.02) µg×cm(-2)×d(-1)]. Within 28 days of dipping, both Fuji VII and Fuji II LC released more F(-) than other materials did(P < 0.05), followed by Compoglass F and Experimental I, and Beautifil and Charisma released the least F(-)(P < 0.01). After F(-) uptake, all materials released the highest F- on the first day and presented sharply decrease after 24 h and slowly decrease after 2 days. On the first day after F(-) uptake, Fuji VII presented the highest F(-) release (>40 µg×cm(-2) ×d(-1)), much more than other materials(P < 0.01), followed by Fuji II LC(>25 µg×cm(-2) ×d(-1)). Beautifil, Compoglass F and Experimental I were close in F(-) release value(15- 20 µg × cm(-2) × d(-1)), and Charisma showed the lowest F(-) release.

CONCLUSIONSTwo glass ionomers were shown to have highest capacity in F(-) release and uptake, followed by compomer and fluoride releasing composite. Composite demonstrated the lowest F(-) release and uptake and Giomer was comparable to composite in F(-) release and to compomer and fluoride releasing composite in F(-) uptake.

Bisphenol A-Glycidyl Methacrylate ; chemistry ; Compomers ; chemistry ; Composite Resins ; chemistry ; Curing Lights, Dental ; Dental Materials ; chemistry ; Fluorides ; pharmacokinetics ; Glass Ionomer Cements ; chemistry ; Resins, Synthetic ; chemistry ; Time Factors