Objective:To study the influence of DiKang capsule on expression of TGF? receptor Ⅱ on hepatic stellite cells(HSC)in rat with liver fibrosis introduced by DMN.Methods:TGF? receptor Ⅱ on HSC in rat with liver fibrosis introduced by DMN were measured by FCM.Results:In group of 10 ?g/kg DMN injected 3 weeks,expression of TGF? recptor Ⅱ on HSC in these rat fed with DiKang capsule 3 weeks were higher than that not feeding capsule.But in group of injecting 5 ?g/kg DMN continuous 6 weeks,expression of TGF? receptor Ⅱ on HSC does not changed with feeding or not feeding DiKang capsule.Conclusion:DiKang capsule may have a certainly role on expression of TGF? receptor Ⅱ on HSC in rat with liver fibrosis introduced by DMN.

Objective To investigate the therapeutic effects of intrasplenically transplanted interferon ? (IFN ?) gene modified hepatocytes on murine implanted liver carcinoma. Methods Murine fetal hepatocytes (BNL?CL2) were transfected with recombinant adenovirus expressing IFN ?. Two cell lines BNL?Lac Z and BNL?CL2 were taken as control. One week after intrasplenically injected C26 cells(colon carcinoma line), sixty tumor bearing syngeneic mice were intrasplenically transplanted IFN ? gene modified hepatocytes and were divided into treated group (BNL?IFN ?) and two control groups (BNL?Lac Z and BNL?CL2), two weeks later, levels of IFN ?, tumor necrosis factor alpha(TNF ?) and nitric oxide(NO) in the serum of liver implanted carcinoma bearing mice were assayed, the cytotoxicity of murine splenic cytotoxic T lymphocyte (CTL) was measured. Mophology of hepatic tumors were studied. The therapeutic effects on the mice with the implanted liver carcinoma were also evaluated. Results In treated group (compared with control groups), the levels of IFN ?, TNF ? and NO in the serum increased significantly ( P

Objective To investigate the expression of interleukin 18 (IL 18) in serum,synovial fluid and synovial tissue of patients with rheumatoid arthritis (RA) and to identify its pathological role in RA.Methods The serum,synovial fluid and synovial tissue were obtained from patients with RA,and samples from patients with osteoarthritis (OA) and from healthy human were used as control groups.Levels of IL 18 protein in serum and synovial fluid were measured by enzyme linked immunosorbent assay (ELISA).Expression of IL 18 mRNA in synovial tissus was determined by semi quantitative reverse transcriptase polymerase chain reaction (RT PCR).The biologic activity of IL 18 in serum and synovial fluid was detected on the basis of IFN ? secretion from IL 18 responding human myelomonocytic KG 1 cells.Expression of iNOS and COX 2 mRNA in synovial tissue was also determined,and productions of NO and PGE 2 in serum and synovial fluid were measured by enzyme reduction method and ELISA.Results Levels of IL 18 protein and biologic activity of IL 18 in both serum and synovial fluid of patients with RA were significantly increased compared with corresponding samples of the two control groups.In addition,the expression of IL 18 mRNA in synovial tissue of patients with RA was also significantly increased compared with samples of the two control groups.Conclusion The over expressed IL 18 may play an important role in the pathogenesis of RA.

OBJECTIVETo investigate the antitumor effects of intrasplenically transplanted interleukin-18 (IL-18) gene-modified hepatocytes on murine implanted liver carcinoma.

METHODSEmbryonic murine hepatocyte cell line (BNL-CL2) was transfected with a recombinant adenovirus encoding IL-18 and used as delivery cells for IL-18 gene transfer. Two cell lines, BNL-LacZ and BNL-CL2, were used as controls. One week after intrasplenic injection of C26 cells (colon carcinoma line), tumor-bearing syngeneic mice underwent the intrasplenic transplantation of IL-18 gene-modified hepatocyte cell line and were divided into treatment group (BNL IL-18) and control groups (BNL-LacZ and BNL-CL2). Two weeks later, the serum levels of IL-18, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in the implanted liver carcinoma-bearing mice were assayed, the cytotoxicity of murine splenic cytotoxic T-lymphocytes (CTLs) was measured, and the morphology of the hepatic tumors was studied to evaluate the antitumor effects of the approach.

RESULTSIn the treatment group, the serum levels of IL-18, IFN-gamma, TNF-alpha and NO increased significantly. The splenic CTL activity increased markedly (P < 0.01), accompanied by a substantial decrease in tumor volume and the percentage of tumor area and prolonged survival of liver carcinomo-being mice.

CONCLUSIONSIn vivo IL-18 expression by ex vivo manipulated cells with IL-18 recombinant adenovirus is able to exert potent antitumor effects by inducing a predominantly T-cell-helper type 1 (Th1) immune response. Intrasplenic transplantation of adenovirus-mediated IL-18 gene-modified hepatocytes could be used as a targeting treatment for implanted liver carcinoma.

Adenoviridae ; Animals ; Cell Line ; Gene Transfer Techniques ; Genetic Therapy ; methods ; Genetic Vectors ; Hepatocytes ; Interleukin-18 ; genetics ; Liver Neoplasms, Experimental ; therapy ; Male ; Mice ; Mice, Inbred BALB C ; Neoplasm Transplantation ; T-Lymphocytes, Helper-Inducer ; immunology ; Transfection

Objective To discuss the influence of bath frequency on neonatal skin condition and body temperature. Methods 108 newborns from obstetrics of People′s Hospital of Peking University during November 2015 to December 2015 were selected, and were randomly distributed t into two groups by lottery. The control group took bath every day, whereas the experimental group did every three days. The newborns were followed up for 4 weeks on their skin condition and body temperature. Then the data were systemized and performed statistical analysis. Results During the first 4 weeks after babies were born, the control group and experimental group showed no significant difference on their skin condition (P>0.05). When compared at the time of first 3 days, 1 week and 2 weeks after the babies were born, body temperature of the experimental group were (36.693±0.182), (36.738±0.174), (36.772±0.185)℃, while the control group were (36.591 ± 0.160), (36.671 ± 0.158), (36.684 ± 0.155)℃,which tended to be significantly lower than the experimental group (t values were -2.697,-2.087,-2.669, P <0.01 or 0.05), but still within the normal range. However, this difference of body temperature disappeared after the first 2 weeks (P>0.05). Conclusions Reducing the bath frequency form once a day to every third day showed no influence on neonatal skin condition. Meanwhile, it did avoid significant reduce of body temperature after bath. This may help improve healthy skin care of newborns, promote their growth, and at the same time, redistribute the clinical nursing work efficiently.

AIM: To evaluate the therapeutic effect and safety of Rhadiola Extract Injection for treatment of stable angina pectoris of coronary heart disease with cariac blood stasis syndrome. METHODS: Arandomized,double-blind,positive drug parallel controlled,multi-center clinical trial was adopted.414 patients with stable angna pectoris of coronary heart disease with cariac blood stasis syndrome were randomly chosen and divided into two groups: test group(n=308 cases) and control group(n=106 cases).The test group was treated with Rhadiola Extract Injection and the control group received Xiangdan Injection.Treatment course of each group was 10 days.(RESULTS:) The therapeutic effect and changes of electrocardiogram in the test group were better than that of the control group(P0.05).The test group had no obvious side-effects. CONCLUSION: Rhadiola Extract Injection is safe and effective in treating stable angina pectoris of coronary heart disease with cariac blood stasis syndrome.

objective To compare three risk prediction system,the physiological and operative severity score for the enumeration of mortality and morbidity(POSSUM),the Portsmouth POSSUM (P-POSSUM)and the colorectal POSSUM(Cr-POSSUM)for the accuracy in predicting operative mortality of patients of colorectal cancer in a single Chinese referral hospital setting. Methods Data of 903 patients,who undergone surgery for colon and rectal cancers from 1992 to 2005 at Peking University Third Hospital,were enrolled in the study.POSSUM,P-POSSUM and Cr-POSSUM was used respectively to predict the mortality rate.ROC curve was applied to judge the differentiation ability of each score.Model goodness-or-fit was tested by the Hosmer-Lemeshow statistic and subgroup analysis was performed by the ratio of observed to expected deaths(O∶E ratio). Results The actual inhospital mortality in our series was 1.0%(9/903).The oredicted mortality rate by POSSUM,P-POSSUM and Cr-POSSUM were 5.6%,2.8% and 4.8%respectively.These predicted mortality rate were significantly higher than actual mortality of our patients.The O∶E ratio was 0.18,0.35 and 0.2 respectively. Conclusion The predicted mortality rate of POSSUM,P-POSSUM and Cr-POSSUM were significantly higher than actual observed mortality rate in a single Chinese referral hospital for patients of colorectal cancer.