Porcelain laminate veneer is one of the most widely used aesthetic dentistry methods at home and abroad now.It has advantages of natural luster of natural teeth,strong abrasive resistance,strong stain resistance,and maximally tooth tissue save,etc.At present,it is widely used in the anterior teeth restoration,and related reports on clinical application of porcelain laminate veneers are increasing day by day.The paper reviews the choice of indications and contraindications,types of materials,tooth preparation methods,and adhensive materials and methods related clinical application questions.

Objective To observe the short-term effect of alprostadil combined with torasemide in the treatment of intractable heart failure.Methods Thirty cases of patients with intractable heart failure and ineffective in routine therapy were given alprostadil and torasemide treatment,with alprostadil 20 mg in 5％ glucose or 0.9％ sodium chloride solution 100 ml intravenous infusion once a day and torasemide 20-40 mg intravenous twice a day 3 days later torasemide dosage was adjusted according to the disease,and the remaining conventional anti-heart failure therapy unchanged.Course was one week.The blood pressure,body weight,urine output,creatinine,electrolytes,N-terminal pro-brain natriuretic peptide (NT-proBNP)and left ventricular ejection fraction (LVEF) before and after treatment was observed.Results After treatment,clinical symptoms of dyspnea,edema,pulmonary rales,such as wet and dry significantly improved in all patients.During the course of the treatment,3 patients appeared mild hypokalemia,and potassium was promptly corrected.Body weight after treatment reduced compared with that before treatment [(63.8 ± 7.6) kg vs.(82.6 ± 10.7) kg],urine output increased after treatment compared with that before treatment [(2 328.3 ±367.8) ml/d vs.(568.7 ± 104.6) ml/d],and the differences were statistically significant (P ＜ 0.01).Systolic blood pressure,diastohc blood pressure difference was not statistically significant after treatment,compared with that before treatment (P ＞ 0.05).LVEF after treatment increased compared with that before treatment [(44.5 ± 8.3)％ vs.(31.9 ± 10.2)％],serum creatinine levels reduced [(97.8 ± 18.6) μmol/L vs.(143.8 ±21.7) μmol/L],and the difference was statistically significant (P＜ 0.05) ; NT-proBNP after treatment reduced compared with that before treatment [(567.4 ± 212.3) ng/L vs.(2 726.5 ± 525.3) ng/L],and the difference was statistically significant(P ＜ 0.01).Conclusion Alprostadil combined with torasemide treatment can quickly and effectively improve symptoms and help improve heart and kidney function in intractable heart failure,and has no significant adverse reactions.

Protein separation method is the core factor of the proteomics strategy. There are three methods for the protein separation, include two dimensional gel electrophoresis, liquid-phase electrophoresis and Mass Spectrometry, and protein-chip. In this article, the principles, processos, and development on the three separation protein methods.were reviewed.

OBJECTIVE In order to prevent and control the medicine source infection,we must clean the palace cavity attraction tubes thoroughly which are repetition use,for antiseptic effect.METHODS Through carries on the sampling,monitor to this hospital′s antiseptic stream of people package of palace cavity attraction tube clean condition.RESULTS Monitoring 100,qualified 91,qualified rate 91%.CONCLUSIONS The palace cavity attraction tube clean process must conform to the standard.Namely:The preliminary clean,adds the enzyme immersion,thorough clean,distilled water flushing.Any remains in the lumen organic matter may affect the disinfection antiseptic effect.

OBJECTIVE To apply and practice the process and effect of low temperature steam formaldehyde(LTSF) sterilization for heat-sensitive goods.METHODS The heat-sensitive medical instruments were sterilized in LTSF sterilizer under the appropriate media conditions,all procedures,including preparation,packing,sterilization,unloading,storage etc were strictly in the right way.RESULTS Sterile result has been guaranteed.CONCLUSIONS LTSF is a kind of effective sterilization method for low temperature sterilization application.By this way,the quality of sterile goods has been reached,the rate of nosocomial infection has been lowered,requirements of hospital clinical departments for sterile goods also have been met.

Objective To compare the efficacy of Si Wu particles to treat young women with chronic attachment inflammation.Methods70 young women with chronic attachment inflammation in department of gynecology from January 2015-July 2016 were grouped by Lottery method.Two groups were treated with levofloxacin and metronidazole, observation group was another given Si Wu particles.To analyze treatment efficacy between two groups.ResultsAfter treatment, total effective rate of observation group was higher than control group(P<0.05).After treatment, whole blood high-viscosity, whole blood low-viscosity and plasma viscosity of observation group were lower than control group(P<0.05).After the third week of treatment, TCM syndrome points of observation group was lower than control group (P<0.05).Adverse reactions and recurrence rate of observation group were lower than control group, but no statistical significance.ConclusionSi Wu particles can improve efficacy to young women with chronic attachment inflammation.

Objective To explore the relationship between hepatic stem cells and hematopoietic stem cells by detecting the expression of OV6 and CD34 in rat hepatic oval cells. Methods Healthy SD rat were fed with chemical carcinogen 3’-me-DAB for 4 weeks, then the rat hepatic tissue samples were obtained. The rats fed with normal forage served as normal controls. The expressions of OV6 and CD34, specific markers for oval cells and hematopoietic stem cells respectively, in rat hepatic stem cells were detected by immunocytochemical staining (ABC method). Results The oval cells positive for OV6 and CD34 were found in the limiting plate of portal area, mainly located around the Hering canal. In addition, some oval cells positive for OV6 and CD34 were found migrating to hepatic lobule, scattering in the hepatic cords. A great many mononuclear stem cells positive for OV6 and CD34 were also found in the hepatic parenchyma. Conclusion 3’-me-DAB could stimulate the proliferation of hepatic oval cells and hematopoietic stem cells and cause the hematopoietic stem cells derived from bone marrow to migrate to hepatic parenchyma and differentiate.

OBJECTIVE:To explore a marketing mode that was in conformity with both market law and consumers'needs for health care products.METHODS:Problems in the marketing of health care products in China were overviewed and the causes were analyzed.RESULTS&CONCLUSION:The short-sighted behavior was a problem that couldn't be neglected in the development of health care products manufacturing businesses.A long-term development mechanism should be established ranging from brand accumulating,mode of marketing,product positioning to after-sale service and etc.

OBJECTIVE:To provide the reference for Chinese pharmaceutical industry in order to satisfy the global phar-maceutical market.METHODS:The development and manufacture of new drugs in today's world market were analyzed in the respect of advancement of technology,medical fee,drug consumption,transferring of research and development center,as well as the development of multinational enterprises.RESULT&CONCLUSION:Great importance should be attached to the development and manufacture of new drugs in today's world market in order to promote the development of Chinese phar-maceutical industry.

Objective To investigate the efficacy and safety of intramuscular injection botulinum toxin A (CBTX-A) made in China. Methods 43 post-stroke patents with upper extremity refractory spasticity were recruited in the study. The patients were randomly divided into two groups: injection combined with rehabilitation therapy (treatment group) and simple rehabilitation therapy (control group). CBTX-A was injected to the biceps brachii. The efficacy was assessed by modified Ashworth scale (MAS), range of motion (ROM),Fugl-Meyer Assessment-upper limb (FMA-upper limb) and motor-Functional Independence Measure-upper limb (mot-FIM-upper limb) at the baseline,2-week,4-week,8-week and 12- week after treatment by a physical therapist who was blind so as to which treatment they received. Results In the treatment group,85.7%(18/21) patients were benefited from the CBTX-A injection. Both groups improved on MAS,ROM,FMA-upper and mot-FIM-upper post-treatment. The score of MAS of treatment group were respectively 1.74?0.41,1.62?0.35,1.60?0.41 at weeks 2,4,8,while that of control group were respectively 2.50?0.51,2.27?0.53,2.18?0.55. The differences were significant(P0.05). After treatment,the ROM of treatment group were respectively 57.81?57.60,66.43?64.38,68.14?65.99 at 4,8, 12-week, and that of control group were respectively 27.91?30. 13,30.73?34.03, 33.73?34.50. The differences were significant (P0.05). Conclusion The result of the present study indicates CBTX-A injection combined with rehabilitation therapy decreases in local spasticity,enlarges the ROM of elbow joint. It is efficient,safe and well-tolerated. CBTX-A injection provides an additional alternative procedure for treatment of adult acquired refractory focal spasticity.