Porcelain laminate veneer is one of the most widely used aesthetic dentistry methods at home and abroad now.It has advantages of natural luster of natural teeth,strong abrasive resistance,strong stain resistance,and maximally tooth tissue save,etc.At present,it is widely used in the anterior teeth restoration,and related reports on clinical application of porcelain laminate veneers are increasing day by day.The paper reviews the choice of indications and contraindications,types of materials,tooth preparation methods,and adhensive materials and methods related clinical application questions.

Objective To explore the relationship between hepatic stem cells and hematopoietic stem cells by detecting the expression of OV6 and CD34 in rat hepatic oval cells. Methods Healthy SD rat were fed with chemical carcinogen 3’-me-DAB for 4 weeks, then the rat hepatic tissue samples were obtained. The rats fed with normal forage served as normal controls. The expressions of OV6 and CD34, specific markers for oval cells and hematopoietic stem cells respectively, in rat hepatic stem cells were detected by immunocytochemical staining (ABC method). Results The oval cells positive for OV6 and CD34 were found in the limiting plate of portal area, mainly located around the Hering canal. In addition, some oval cells positive for OV6 and CD34 were found migrating to hepatic lobule, scattering in the hepatic cords. A great many mononuclear stem cells positive for OV6 and CD34 were also found in the hepatic parenchyma. Conclusion 3’-me-DAB could stimulate the proliferation of hepatic oval cells and hematopoietic stem cells and cause the hematopoietic stem cells derived from bone marrow to migrate to hepatic parenchyma and differentiate.

OBJECTIVE:To discuss on the relationship between the research and development of new drugs and the global demands.METHODS:The demands of the world's drug market were discussed.The important issues in the research and development of the new drugs were analyzed and the thoughtful insights into these issues were put forward.RESULTS&CONCLUSION:The research and development should be aimed for drug variety,dosage form,the designs of package and label and drug registration in order to meet the demands of globalization.

OBJECTIVE:To explore a marketing mode that was in conformity with both market law and consumers'needs for health care products.METHODS:Problems in the marketing of health care products in China were overviewed and the causes were analyzed.RESULTS&CONCLUSION:The short-sighted behavior was a problem that couldn't be neglected in the development of health care products manufacturing businesses.A long-term development mechanism should be established ranging from brand accumulating,mode of marketing,product positioning to after-sale service and etc.

Objective To investigate the effect of ethyl pyruvate (EP) on the expression of caspase-3 in the hippocampus and the learn-ing and memory ability in neonatal rats with hypoxic ischemic brain damage (HIBD). Methods 90 Wistar rats of 7 days old were randomly divided into sham operation group (n=15), HIBD group (n=15), EP1 group (n=15, 10 ml/kg), EP2 group (n=15, 30 mg/kg), EP3 group (n=15, 50 mg/kg) and EP4 group (n=15, 100 mg/kg). The model was established with Rice's method. 30 minutes before operation, and every 24 hours after operation, EP groups were injected with 10 ml/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg EP in abdomen respectively, for 2 weeks. Af-ter treatment, the caspase-3 positive cells were observed by immunohistochemical staining, and the latency and the times crossing the target quadrant were tested by Morris water maze test. Results The caspase-3 positive cells were less in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). The latency and the times crossing the target quadrant were better in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). Conclusion Ethyl pyruvate can de-crease the expression of caspase-3 in hippocampus, and improve the ability of memory and learning in neonatal rats with HIBD.

Objective To explore the effect of the different doses of oxytocin(OXT) on stress model aggressive behaviour in Wistar rats.Methods According to random number table,40 pregnant rats were divided into experimental stress groups (A, B, C) and control group(D) with 10 samples in each group.Before forced swimming test, rats in experimental groups (A, B, C)were given OXT via intraperitoneal injection with 0.3 mg/kg, 1.0 mg/kg,2.0 mg/kg;rats in group D were given equal volume of normal saline in the same way.At the 3rd day all rats were tested with maternal aggression,including mothers' following indices as total aggression times,biting times,pinning times,attack latency and duration of attack.Elevated plus maze (EPM) test was applied to analyze the behavioral changes.Results In terms of the duration of attack and attack latency,the indexes of group A and group D were significantly different from relevant data ((147.60±23.92) s and(79.70±9.88) s, P＜0.05), while other indexes had no difference(P＞0.05).The duration of attack and attack latency had a significant difference ((3.10±0.87) times, (13.60±5.14)times, (91.30±9.74)s and(167.20±30.02) s, P＜0.01) between group B and group D except total aggression times(P＞0.05).Group C and group D in given indexes had significant differences showing (6.40± 1.34) times, (15.10±4.35) times, (23.70±3.46) times, (49.80± 5.53) s and (215.60± 39.55) s (P＜0.01).Group A and group B in given indexes had significant differences (P＜ 0.05);and group B and group C had a significant difference (P＜0.01)except pinning times(P＞0.05).In EPM ,the open arm entry ratio and the open arm time ratio of both group A and group D were significantly different from respective percentage (0.47±0.13) and (0.13±0.05) (P＜0.01).The significant difference was found in group B and group D(P＜0.01);group A and group B had a statistical difference(P＜0.05);there was no difference in both groups in open arm time ratio(P＞0.05).Conclusion Oxytocin potentially mediates maternal aggression by attenuating stress, and improves the intimate bonding between mother and pups,which means dose dependency of OXT in this process.

Objective To investigate the efficacy and safety of intramuscular injection botulinum toxin A (CBTX-A) made in China. Methods 43 post-stroke patents with upper extremity refractory spasticity were recruited in the study. The patients were randomly divided into two groups: injection combined with rehabilitation therapy (treatment group) and simple rehabilitation therapy (control group). CBTX-A was injected to the biceps brachii. The efficacy was assessed by modified Ashworth scale (MAS), range of motion (ROM),Fugl-Meyer Assessment-upper limb (FMA-upper limb) and motor-Functional Independence Measure-upper limb (mot-FIM-upper limb) at the baseline,2-week,4-week,8-week and 12- week after treatment by a physical therapist who was blind so as to which treatment they received. Results In the treatment group,85.7%(18/21) patients were benefited from the CBTX-A injection. Both groups improved on MAS,ROM,FMA-upper and mot-FIM-upper post-treatment. The score of MAS of treatment group were respectively 1.74?0.41,1.62?0.35,1.60?0.41 at weeks 2,4,8,while that of control group were respectively 2.50?0.51,2.27?0.53,2.18?0.55. The differences were significant(P0.05). After treatment,the ROM of treatment group were respectively 57.81?57.60,66.43?64.38,68.14?65.99 at 4,8, 12-week, and that of control group were respectively 27.91?30. 13,30.73?34.03, 33.73?34.50. The differences were significant (P0.05). Conclusion The result of the present study indicates CBTX-A injection combined with rehabilitation therapy decreases in local spasticity,enlarges the ROM of elbow joint. It is efficient,safe and well-tolerated. CBTX-A injection provides an additional alternative procedure for treatment of adult acquired refractory focal spasticity.

Objective To explore the clinicopathological features,diagnosis and differential diagnosis of colonic muco-submucosal elongated polyp (CMSEP).Methods The clinical manifestations,endoscopic and histological features of three cases of CMSEP were analyzed.The literature was reviewed with regard to the diagnostic criteria and differential diagnosis.Results Three patients were male,and aged 25,45 and 75 years old.Two polyps were at transverse colon and one at rectum.Under colonoscope,all three cases showed the long pedunculated polyps.The surfaces of two polyps were smooth and the colors were similar to colonic mucosa while one polyp appeared mucosal erosion and congestion on the top.Under optical microscope,three cases had similar lesions.The axis of the lesions was expanded submucosa and the surface was the layer of colonic mucosa.The expanded and thick walled blood vessels were seen paralleled to the long axis in the submucosa of the axis of lesions.One polyp with mucosal erosion on the top presented with adenocarcinomal changes.Conclusions CMSEP is a kind of specific polypoid lesion,with characteristic colonoscopic and pathological features.Most are benign changes,however malignant changes occur in a few cases.In histology,CMSEP should be identified with mucosal prolapse syndrome associated inflammatory polyp.

Objective: To study the clinical effect of nursing after laparoscopic myomectomy after nursing intervention. Methods: Retrospective analysis of our hospital from 2009 May to 2012 October in our department, laparoscopic myomectomy in the treatment of 148 cases of uterine fibroids patients clinical data, were randomly divided into 2 groups, 74 cases in each group, the control group routine nursing care to patients, nursing intervention on the patients with study group, The quality of life of patients with depression and anxiety scores were compared between the two groups before and after the rate and nursing of exhaust and ambulation time, hospitalization time, complications. Results: Research group of exhaust, ambulation, time of hospitalization, the incidence of complications compared with the control group, showed significant difference; the study group before operation and nursing of patients with anxiety and depression and quality of life scores compared with the control group, there was no significant difference, nursing intervention, there were significant differences. Conclusion: Laparoscopic myomectomy nursing intervention, can promote the rehabilitation, and improve the living condition, patients with significant effect, should be popularized.