Objective To investigate the inhibition of Sophora flavescens extract on the degree of inflammatory swelling of guinea pigs'skin with eczema, observe pathological changes and explore the effects of the anti-in-flammatory action, in order to provide experimental basis for clinical treatment.Methods Eczema models of 40 guinea pigs were set up by smearing 2, 4-dinitrochlorobenzene on their napes to cause sensitization and motivating in the inside of their right ears.All guinea pigs were randomly divided into 5 groups equally,including the model com-pared group, positive control group and high, medium, low concentration groups of sophora flavescens.After eczema skin was continuously administered drugs for 14 days, the same part with equal area of the left and right ears was taken and weighed.The difference between the left and right ears was used to evaluate the swelling degree of skin inflammation.The pathological changes were observed by making pathological slices with HE staining with the tissue blocks of right ears.Results Compared with the model group, the swelling degree of skin inflammation was significantly relieved and dermal edema and inflammatory cell infiltrating were significantly improved in the high and medi-um concentration groups of sophora flavescens (p<0.05).Compared with the positive group, the swelling degree of skin inflammation, dermal edema and inflammatory cell infiltrating were significantly alleviated in the high concentra-tion group of sophora flavescens ( p<0.05) .Conclusion Sophora flavescens extract can effectively reduce inflam-matory response of guinea pigs'skin with eczema, having a certain significance for the clinical treatment of eczema.

The innovation of medical technology and progress of health care are dependent on the effectiveness of hospital scientific research management.By integrating the concept of refined management and with the construction of research platforms,an innovative administration mode for hospital research management was established and a sustainable development of hospital scientific research administration becomes possible.

Objective:To investigate the availability for rhIL-18 in the in vitro culture system to stimulate PBMHCs,with inducing cytotoxicities against tumor cells and to analyze the influencing factors for the effects.Methods:The NK cells,T cells and dendritic cells were separated from fresh PBMNCs by using the Stem Sep TM immunomagnetic beads.Cell phenotypes of the purified cell populations were identified with FCM technique.The cell co-culture system was established as follows.The PBMNCs or the cell preparations deleting the definite cell subset with immunoscreening were co-cultivated with mitotic-inactivated tumor cells in the presence of rhIL-18.Cytotoxicities of the various effector cell preparations to a series of tumor cell lines were examined by the isotope releasing assay.Results:In the in vitro cell co-culture system,rhIL-18 rapidly induces activation of the cytolytic responses against various tumor cell lines mediated by PBMNCs.The rapid induction within 24 h of culture was dependent on the dosage of rhIL-18,with the optimal dose of 100 ng/ml of the cytokine.The activated cytotoxicities were abrogated by deleting NK cells prior to the cell co-culture but did not vary when either T cells or DCs were removed.The cytotoxic responses were shown as a pattern of broad-spectrum to the target cells used and were not blocked by anti-MHC moAbs.Conclusion:NK cells were responsible for the rapid induction of cytotoxicities against tumor cells by rhIL-18.

Objective To comparatively analyze the DNA extracted from sweat latent fingerprints on the adhesive side of tapes by three kinds of methods. Methods DNA was extracted from sweat latent fingerprint on the adhesive side of tapes using silicon bead test,QIA micro Kit and combined silicon bead-QIA micro Kit. STR loci were detected by multiplex PCR procedures. The PCR product was electrophoresed on an ABI 3100 Genetic Analyzer. Results 36%of the sweat latent fingerprints on the adhesive side of tapes was successfully genotyped using combined silicon bead-QIA micro Kit, and 21% using QIA micro Kit. Conclusion DNA genotyping of the sweat latent fingerprints on the adhesive side of tapes reveals higher possibility using combined silicon bead-QIA micro Kit than using QIA micro Kit and is less time-consuming.

OBJECTIVE: To study the drug resistance of baumanii so as to provide references for rational use of antibiotics in the clinic. METHODS: The susceptibility test results of 270 strains of baumanii isolated from clinical samples in our hospital during the period of 2001-2004 were analyzed statistically. RESULTS: The isolating rate of baumnii stood at 16.6% , which had no drug resistance strains to piperacillin/tazobactam; its drug resistance rates to lmipenem and ampicillin/sulbactam stood at 16.3% and 22.6%, respectively; its drug resistance rates to amoxicillin/ clavulanic acid stood at 33.3%; which had higher drug resistance rates to the other antibacterials, particularly to cephalosporins. CONCLUSIONS: To reinforce the monitoring on the drug resistance of baumanii and to standardize the application of antibacterials are helpful for the maintaining of antibacterial activity of sensitive antibacterials.

0.05).FVC increased significantly in week 6 and 12(t=2.762,P =0.008;t=2.255,P =0.029,respectively).There was no statistical significance in FEV_1/FVC between two groups.IC increased significantly in week 6 and 12(t =3.204,P =0.002;t =3.109,P=0.003,respectively).Conclusion Inhaling tiotropium increases IC in patients with stable COPD.As lung function targets judging limit extent of airflow in patients with COPD,FEV_1/FVC and FEV_1(%)are not sensitive in evaluating therapeutic effect of bronchodilators.IC is more reliable than other targets in evaluating therapeutic effect of bronchodilators.

Objective To evaluate the effect of pituitrin and nursing for patients with vasoplegic syndrome after cardiopulmonary bypass. Methods 38 patients with cardiopulmonary bypass after open heart operation were divided into two groups with 19 cases in each group according to odd-nunbered and even-numbered days, the PT group was given pituitrin treatment, the NE group was given norepinephrine treatment. Heart rate, MAP, urine volume were recorded at 0.5, 1, 2, 6, 12 and 24 hours after drug therapy.Mechanical ventilation time, ICU stay time,hepatic and renal function and clinical symptom were compared between the two groups. Results MAP and urine volume increased significantly at 0.5 hour after pituitrin therapy. MAP increased from (55.33±4.10)mmHg to (67.34±4.13)mmHg. Urine volume increased from (0.08±0.02)ml·kg-1·h-1 to (1.84±1.21)ml·kg-1 ·h-1 MAP and urine volume also increased at other time points. In the NE group, MAP increased at 1h after treatment. Both MAP and urine volume increased at 2h and later after norepinephrine treatment. One death happened in the NE group. Compared with the NE group, the PT group was associated with lower ICU stay, mechanical ventilation time and 24h serum LAC.Liver and kidney function, myocardial infarction, nausea and vomiting, and other side effects were not different between the two groups. Conclusions Application of low-dosage pituitrin is rapid, efficient and safe treatment for patients with vasoplegic syndrome after open heart operation.

Objective To investigate the changes of serum T helper cell (TH)1/TH2 type cytokines after the active immunotherapy in unexplained habitual abortion (UHA) women. Methods Concentrations of interleukin (IL) 2, IL 12, interferon (IFN) ?, IL 4, IL 10 and transforming growth factor (TGF) ?1 were measured by enzyme linked immunosorbant assay (ELISA) method in sera from thirty three cases of unexplained habitual abortion (UHA) women before and after active immunotherapy. Thirty normal non pregnancy (NNP) women and thirty normal pregnancy (NP) women were taken as control. Results (1) Serum concentrations of IL 2 and IL 12 were higher significantly (P

Objective To evaluate the clinical significance of laparoscopic resection for large intramural hysteromyoma. Methods A retrospective analysis was made concerning 42 cases of single intramural hysteromyoma as large as 6～10 cm in diameter: 24 cases underwent laparoscopic hysteromyomectomy and 18 cases received open resection. Intra- and post-operative parameters between the two groups were compared. Results All operations in the two groups were successfully completed without complications. The operative time was significantly longer in the laparoscopic group (89.0?26.9 min) than that in the open group (63.3?20.1 min) ( t=3.400,P =0.002). No statistical difference was observed in the intraoperative blood loss between the laparoscopic group (93.6?65.9 ml) and the open group (100.0?48.7 ml) ( t=-0.347, P=0.731) . The analgesic requirement was less in the laparoscopic group (2 out of 24 cases ) than that in the open group (9 out of 18 cases) ( ? 2 =7.208, P =0.007). The time to first flatus was shorter in the laparoscopic group (23.5?11.3 h) than that in the open group (32.0?13.6 h) ( t=-2.211, P =0.033). The postoperative pyrexia rate was significantly lower in the laparoscopic group (2/24) than that in the open group (7/18) ( ? 2=4.033, P =0.045). Conclusions Laparoscopic resection for larger intramural hysteromyoma is safe and reliable. As compared with open hysteromyomectomy, it offers more rapid recovery and lower postoperative pyrexia rate, as well as the same amount of blood loss. Its prolonged operative time may be associated with the relatively large size of the hysteromyoma.

Objectives　To investigate the relationship between homocysteine (Hcy), folic acid, vitamin B12(VitB12),lipids and the carotid artery atherosclerosis(CAAs) and the significance of their levels in predicting cardiac and cerebral vascular events of older patients. Methods　Both sides of the common carotid artery and internal carotid artery were measured by B-mode ultrasound in 74 examinees in A, B, C, and D groups and a control group. With fasting serum, cholesterol, triglyceriade, apolipoprotein AI(Apo AI), apolipoprotein B (Apo B), lipoprotein (a) ［Lp(a)］, creatinine, glucose, folic acid, Vit B12 and Hcy were detected. Results　When carotid artery atherosclerosis became severer, serum Hcy was higher. The levels of the control and A, B, C, D groups were (10.2±3.6) μmol/L, (11.5±4.4) μmol/L, (17.9±4.5) μmol/L, (24.7±10.3) μmol/L, and (41.4±22.3) μmol/L respectively. SNK test showed a significant difference between D and other groups (P>0.05). Folic acid and Vit B12 were negatively correlated with the severity of carotid artery atherosclerosis, but only A and D groups showed statistical significance. Spearman correlation coefficient showed that serum Hcy had significant negative correlation with folic acid and Apo AI(P=0.035 and P=0.000).Multiple linear regression analysis between CAAs and various risk factors showed Apo AI had negative correlation, but Apo B, Lp(a) and Hcy had positive correlation with CAAs, especially Hcy(P=0.009).Conclusion　Hyperhomocysteinaemia is an important risk factor of artery atherosclerosis and the degree of CAAs is highly correlated with Hcy concentration. Hcy, Apo AⅠ,Apo B, Lp(a) are sensitive and reliable indexes for evaluating and predicting CAAs.