BACKGROUND: Nerve growth factor (NGF) plays a regulatory effect on survival, growth, differentiation, and egeneration of neurons. OBJECTIVE: To verify the therapy effects and the expression of Fos protein with different dose of NGF on the spinal cord of rats with neuropathic pain.DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Shanghai Ninth People's Hospital.MATERIALS: A total of 72 male, health, adult, Wistar rats, weighing 180-200 g, were randomly divided into 4 groups, namely,model group and NGF with 4, 8, 16 Ijg groups, with 18 animals in each group.METHODS: All rats were prepared sciatic nerve chronic constriction injury models. Rats were intraperitoneal injected NGF with 4,8, 16 μg/(kg·d) in the experimental groups.MAIN OUTCOME MEASURES: ① Behavioral observation and mechanical pain threshold testing was performed prior to and at days 1,2, 3, 5, 7, 10, 14 after operation. ②Six of rats in each group were sacrificed at days 2, 7, and 14 after operation, and the expression of Fos protein in the spinal cord were assayed with the immunohistochemicat methods and image analysis.RESULTS: In the model group, rats raised and licked feet spontaneously after operatiOn, and the mechanical pain threshold was decreased from the 3rd day, and reached the lowest at day 14. However, in the NGF groups, rat exhibited no pontaneous pain,without subnormal period of mechanical pain threshold. The mechanical pain threshold in rats of the model group as significant different to the other groups at days 10 and 14 after operation (P < 0.05). The mechanical pain threshold in rats of all groups increased, but which of 16 μg NGF group was lower than the other groups on the first day after operation (P < 0.01 ) and lower than that of the 4 μg NGF group at 2 days after operation (P < 0.05). The expression of Fos protein in the spinal cord of the model group was increased than that of the other groups after operation (P < 0.01 ). The expression of Fos protein in the spinal cord of the 16 μg NGF group was less than that of model and 4 μg NGF groups at day 2 after operation (P < 0.01 ).CONCLUSION: NGF has therapeutical effect on neuropathic pain of rats, especially with large dosage. The mechanisms may be inhibiting the expression of Fos protein in the spinal cord of rats.

Objective To determine the association of anomalies of female reproductive system with that of urinary system. Methods Two hundred and eighty-nine cases, including 59 with congenital vaginal inclined septum syndrome, 135 with septate uterus, 4 with unicornuate uterus, 13 with rudimentary horn of uterus, 58 with congenital absence of the uterus and vagina, 14 with imperforated hymen, and 6 with atresia of vagina, treated during the past 4 years, were analysed retrospectively. Results Anomalies of urinary system were more frequently found in patients with anisomerous anomalies of reproductive system(85. 53% , 65/76 cases). In 59 cases of congenital vaginal inclined septum syndrome, 43 had ipsilateral renal agenesis, 2 had one single pelvic kidney, 1 had bilateral horseshoe kidneys, 1 had left-side horseshoe kidney, 1 had one pair of renal pelvis and renal calices with ureters incorporated at middle and inferior segments on both sides. Ipsilateral renal agenesis was also seen in patients with rudimentary horn of uterus(14/14 cases) and unicornuate uterus(4/4 cases). However, there was no urinary system malformation seen in septate uterus patients (0/135 cases), imperforated hymen (0/16) and atresia of vagina (0/4 cases). Only 1 case of congenital absence of the uterus and vagina associated with ipsilateral renal agenesis. Conclusion Anomalies of reproductive ducts associated with renal malformations, especially anisomerous anomalies of reproductive system are not unusual. Early recognition and diagnosis is very important to prevention and treatment of such diseases.

Objective To find out about the attitudes of hospital runners, academic leaders and key members of the technical force in public hospitals about structural reform in their institutions and to explore modes and methods for such reform. Methods Survey questionnaires were designed, anonymous surveys were conducted on the spot, and statistical analyses were made of the data collected. Results Surveys on 1904 people in 55 second- and third-tier hospitals indicated that 84.9% were in favor of structural reform in their institutions; 89.7% played an active part in the reform; 86.8% held that structural reform should first be carried out in large and medium-sized hospitals; 96.6% were in favor of such reform modes as government-controlled stock systems, government-owned and civilian-run hospitals, and non-governmental hospitals, with 66% of them supporting the mode of government-controlled stock systems. Conclusion The majority of hospital runners, academic leaders and key members of the technical force in public hospitals actively support structural reform in their institutions and their views with regard to the modes and methods for such reform are in accordance with the guidelines put forward by the government. Sound ideological foundations have been laid for duly promoting structural reform in public hospitals.

Objective To investigate the changes of serum T helper cell (TH)1/TH2 type cytokines after the active immunotherapy in unexplained habitual abortion (UHA) women. Methods Concentrations of interleukin (IL) 2, IL 12, interferon (IFN) ?, IL 4, IL 10 and transforming growth factor (TGF) ?1 were measured by enzyme linked immunosorbant assay (ELISA) method in sera from thirty three cases of unexplained habitual abortion (UHA) women before and after active immunotherapy. Thirty normal non pregnancy (NNP) women and thirty normal pregnancy (NP) women were taken as control. Results (1) Serum concentrations of IL 2 and IL 12 were higher significantly (P

Objective:To explore relation of TCM syndrome types in patient with sexual hormones of hyperplasia of mammary glands,so as to establish objective indexes of TCM syndrome types of hyperplasia of mammary glands.Methods:112 cases of hyperplasia of mammary glands were divided into 3 types:stagnation of liver-Qi,intermingled phlegm and blood stasis,disharmany of thoroughfare vessel and conception vessel.Level of estradiol(E_2),progestin(PT),testosterone(TT),prolactin(PRL),follicle- stimulating hormone(FSH)and luteotropic hormone(LH)during ovulatory period in the patients were determined.The relation of hormone level with TCM syndrome types was analyzed.Results:Level of LH and E_2 decreased,PRL and PT increased and level of TT and FSH did not significantly change during ovulatory period in the patient of hyperplasia of mammary glands.LH and E_2 level in the patients with stagnation of liver-Qi or intermingled phlegm and blood stasis was significantly lower than normal values,and the level of PRL and PT was significantly higher than that of normal values.The level of LH and E_2 was also lower than the normal value in the patient with disharmany of thoroughfare vessel and conception vessel,but the level of FSH was significantly higher than normal value.Conclusion:The patient of hyperplasia of mammary glands during ovulatory period manifests endocrine dysfunction,and the patients with different TCM syndrome are different in secretion of sexual hormones; Changes of sexual hormones can be used as objective indexes of TCM syndrome types and basis of clinical medicinal application.

Objective To investigate the long-term protective effects of the waistband onto lumbar spins.Based on the practical situation,to search for some effective and convenient methods,such as wearing waistband and mutual massage,to prevent from lumbago for the army drivers worked in plateau.Methods 372 army drivers,young males,healthy and all worked in plateau,ware involved as the treatment group in the present study.Another 545 army drivers with the similar physical qualities,also worked in plateau,were selected as the control group.For the treatment group,a kind of special waistband was employed for the drivers,and mutual massage on hack of each other was performed among them;while in control group the army drivers were given no measures for lumbar protection and lumbago pre- vention.All the drivers in the both groups ware in normal driving conditions.The therapeutic effects of the waistband and the lumbago symptoms were investigated by epidemiologic survey 16 months later,Results The epidemiologic survey results showed that the incidence of lumbago in the drivers of control group(72.8%)was significantly higher than that in the treatment group(30.9%,P

Nerve growth factor (nerve growth factor, NGF) is the earliest discovered growth factor in neurotrophic factors,it has a very important role in maintaining the survival, growth, differentiation,repair and regeneration after injury of neurons. In this paper,research progress in recent years on NGF are reviewed.

Objective To observe the changes in cytokines, oxygen free radicals, acidulous production in serum and to investigate the effectiveness of monitoring metabolism of cells and the method for evaluating cell injury in patients with severe trauma. Method The detailed data of 117 patients rescued and managed carefully from May 2005 to February 2007 were assessed and stratified with ISS and APACHE Ⅱ , and the serum levels of arterial blood lactate(ABL) ,base deficit(BD) ,superoxide(SOD) ,lipid peroxide(LPO) ,TNF-α and IL-6 were measured in real-time according to the condition of the patient. Results The monitoring biomarkers obviously changed with injury severity which endangered the situation of patients after trauma (P < 0.05) , especially in the patients with ischemia , hypoxia, shock, iniection, SIRS, and MODS (P < 0.01). The persistence of extremely elevated levels of biomarkers meant the organ failure and fatality of patients after trauma, and there was a obvious differece between those cases and cases without elevation of biomarkers ( P < 0.01). Conclusions The outcome of patients after trauma is closely correlated with injury severity,infection and MODS,and the levels of biomarkers including ABL, SOD, IPO, TNF-a and IL-6 are useful indicators of outcome measure.

AIM To study the effects of poly- N -acetylglucosamine on the metabolism of low density lipoprotein and lipoprotein (a). METHODS Poly- N -acetylglucosamine was injected into the body of hedgehogs viathe armpit vein 2 minutes before the 125 I low density lipoprotein ( 125 I-LDL), 125 I lipoprotein(a) 〔 125 I-Lp(a)〕, 125 I desialylated low density lipoprotein ( 125 I-ds-LDL) or 125 I desialylated lipoprotein(a) 〔 125 I-ds-Lp(a)〕 were injected separatively . The animals were put to death in one hour. The radioactivity in the blood, liver, kidney, spleen, gall and adrenal were measured. RESULTS Absorption of ds-LDL and ds-Lp(a) increased in the liver. Later ds-LDL was reutilized and released into blood, but the majority of ds-Lp(a) was excreted out of the body from liver. It was not eliminated that ds-LDL was metabolized via the LDL receptor for the uptake of ds-LDL also increased in the kidney and the adrenal. It is possible that ds-Lp(a) and the part of ds-LDL may be catabolized via the desialylated lipoprotein-related receptor. Poly- N -acetylglucosamine could increase the absorption of LDL in the liver and the adrenal, meanwhile the concentration of LDL decreased in the blood and the absorption of LDL decreased in the spleen. CONCLUSION Poly- N -acetylglucosamine has the effects of lowering lipids through activating LDL receptor.

Objective To investigate the therapeutic efficiency of intravenous implanted endothelial progeni-tor cells (EPCs) pretreated with stromal cell derived factor-1 (SDF-1) in rat model of acute myocardial infarction (AMI). Method The bone-marrow derived EPCs were cultured. The effects of SDF-1 on the migration and sur-rival of EPCs were evaluated in vitro. The rat models of AMI were produced and randomly divided into SDF-1 +EPCs group (n=12), EPCs group (n=12) and control group (n=12). EPCs pretreated with or without SDF-1 were infused via tail vein 24 hours after AMI modelled. The EBM-2 culture medium without cell was infuaed in control group. Vessel density, cardiac function and infarct area were measured on 14 days and 28 days after cell implantation. Results Fourteen days after cell implantation, the vessel density of SDF-1+EPCs group was higher than that of EPCs group and control group. Twenty-eight days after cell implantation, the cardiac function of SDF-1+EPCs group was better than that of EPCs group and control group. Conclusions EPCs pretreatment with SDF-1 can improve the survival and migratory capacity of EPCs, and increase the therapeutic efficiency for myocardial infarction.