1.Screening of proteins interacting with HSPC016 by yeast two-hybridization technique
Zhiqiang SONG ; Lihua SUN ; Fei HAO
Chinese Journal of Dermatology 2011;44(1):44-46
Objective To screen and identify proteins interacting with hematopoietic stem/progenitor cell differentiation-related gene HSPC016, and to explore the molecular mechanism involved in the regulation by HSPC016 on the aggregative behavior of dermal papilla cells. Methods By using yeast two-hybridization,HSPC016 gene was sub-cloned into pGBKT7 to construct the bait plasmid (named as pGBKT7-HSPC016) in yeast AH109. The cDNA yeast expression library of human dermal papillae cells in yeast Y187 was screened with the bait plasmid and the proteins interacting with HSPC016 were identified. Yeast two-hybridization retransformation experiment was conducted to exclude the false positive clones and verify the interactions, then,the positive clones were sequenced and analyzed by using bioinformatic methods. Results The bait plasmid pGBKT7-HSPC016 was constructed successfully and there was no self-activation in or toxicity against yeast AH 109. Four proteins,including forkhead family of transcription factors (FOXO 1 ), mitogen-activated protein kinase 11 (MAPK 11 ), phosphoinositide-3-kinase (PIK3R3) and liver X receptor were screened and identified. Bioinformatic analysis revealed that these proteins had close relationship with intracellular energy metabolism and translational regulation. Conclusions HSPC016 may regulate the aggregative behavior of DPCs by regulating the levels of intracellular reactive oxygen species (ROS) and interacting with signaling molecules involved in intracellular energy metabolism and translational regulation.
2.Recent advances in understanding the relationship between long non-coding RNA and atherosclerosis
Lihua ZHUANG ; Jiacai HU ; Hao WU
Chinese Journal of Geriatrics 2016;35(3):329-333
Long non-coding RNAs (lncRNA) play important roles in many biological processes.However,compared with other types of RNAs,limited research has been conducted on lncRNA,mostly concerning its relationship with tumor,and there are much fewer studies on its relationship with atherosclerosis.In this article,we summarized the latest research findings in this field,with an emphasis on the importance of IncRNA in atherosclerosis.The value of lncRNA in targeted therapy of atherosclerotic disorders is also discussed.
3.Determination of Picamilon in Human Plasma by LC-MS
Lihua JIA ; Guangtao HAO ; Hongzhi GAO
China Pharmacy 1991;0(02):-
OBJECTIVE:To establish an LC-MS method for determination of picamilon in human plasma. METHODS:Picamilon and repaglinide(as internal standard)were separated on Agilent C18 column,using methanol-water(85 ∶ 15) as the mobile phase at a flow rate of 0.2 mL?min-1. Column temperature was set at 20 ℃. SIM was used for determination of picamilon in human plasma.Ion mass spectral(m/z) of 207.0→121.1 was selected for picamilon and 451.3→379.2 for repaglinide.RESULTS:The linear range was 50~10 000 ?g?L-1(r=0.999 1); the method recovery was within 99.14%~104.27%. The RSD of inter-day and intra-day validation were 2.12%~5.94% and 7.30%~8.44%,respectively. CONCLUSION:The method is sensitive and accurate for the determination of picamilon in human plasma and its pharmacokinetic study.
4.Analysis of risk factors for young adult stroke
Hao ZHANG ; Lihua CUI ; Xiaonian ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2003;9(4):244-245
ObjectiveTo evaluate the risk factors of young adult stroke.MethodsTo analyze the risk factors of 203 young adult stroke. The risk factors included hypertension, lipidemia, diabetes, heart disease, TIA, family history, tobacco and/or alcohol use.ResultsHypertension was still the most frequent risk factor. Diabetes, heart disease and TIA were not much more important in young adult with stroke. Almost half of the young adult stroke had family history and hobbies of smoking and drinking.ConclusionsHypertension is an important risk factor in young adult stroke. Other risk factors such as low level of HDL, smoking, drinking and family histories still contribute to the causes of young adult stroke.
5.Large sample clinical study on the safety and efficacy of recombinant human growth hormone in the treatment of pediatric growth hormone deficiency
Hui ZHAO ; Bing WANG ; Hao WEI ; Hai SHENG ; Lihua SONG
Chinese Journal of Primary Medicine and Pharmacy 2012;19(10):1456-1458
ObjectiveUsing large sample clinical study to reevaluate the safety and efficacy of recombinant human growth hormone(ANSOMONE) in treating pediatric growth hormone deficiency(GHD).Methods1012 cases were administered with ANSOMONE at a dose of 0.1 IU · kg-1 · d -1 in bedtime for 6 months.The alteration of mean height,growth rate,height standard deviation scores,bone age,and anti-GH antibody and occurrence of adverse effects were observed.ResultsThe growth rate was promoted from ( 2.50 ± 0.85 ) cm/year to ( 13.44 ± 4.27 ) cm/year,the net growth rate was( 10.94 ±3.69)cm/year.There was significant difference between the pre-treatment and post-treatment patients ( P < 0.01 ).The post-treatment anti-GH antibody positive rate was 13.34%,which had no obvious effect on the efficacy.Few patients exert transient inflammation reactions,which disappeared with any treatment.ConclusionANSOMONE had significant efficacy,and could promote the height increase obviously.It had good safety without any obvious side effect and early-maturing effect.
6.Practice and perspective of delicacy management in public hospital
Yang ZHAO ; Lihua YI ; Minmin HU ; Aimin HAO
Chinese Journal of Hospital Administration 2011;27(9):653-657
The authors probed systematically into the delicacy management measures adopted by the hospital in its internal mechanism reform, innovative medical service model, human resource optimization, and its performance appraisal system reform. Discussions in the paper include such topics as flat management for hospitals,intensification,centralization and integration in diagnose and treatment, as well as vertical nursing management, and the outsourced logistic services. The methods and paths to achieve these goals are summarized as “preciseness, accuracy, carefulness, and strictness”.
7.Reform attempts for performance and remuneration management by implementing balance score card
Lihua YI ; Aimin HAO ; Ying ZHOU ; Minmin HU ; Huikang YUAN
Chinese Journal of Hospital Administration 2011;27(10):721-723
The reform of performance appraisal and salary system is key to public hospitals reform.This paper introduced practices of the factor performance and salary system reform based on the Balance Scorecard in hospitals.It established a four-dimension performance appraisal structure,a motive salary allocation model covering all members of a hospital.This would help build the performance appraisal and salary allocation system meeting present needs of public hospitals in China.
8.Expression of estrogen receptor in entopic and ectopic endometrium of abdominal wall endometriosis
Yutun HAO ; Libing WANG ; Xueling LIU ; Lihua PEI
Chinese Journal of General Practitioners 2009;8(7):484-485
Fifty-six patients with abdominal wall endometriosis(AWE)were assigned into the proliferous group or the secretory group and the expression of estrogen receptor(ER)in the entopie,ectopic and normal endometrium was investigated.In the proliferous group,the positive ER expression rate on the entopie endometrium(85%)was significantly higher than that on the normal(60%)or ectopie endometrium (58%)(P<0.05).In the secretory group,the positive ER expression rate on the eetopie endometrium (57%)was significantly higher than that on the entopic (26%) or normal endometrium(25%)(P< 0.05).There was significant difference in the entopie and normal endometriilm between the two groups (P<0.01):however,there was no significant diffeFence in the ectopic endometrium.Abnormal ER expression on the entopic or ectopic endometrium may play a role in the pathogenesis of AWE.
9.Steps to further reform the public hospital system
Lihua YI ; Minmin HU ; Yang ZHAO ; Aimin HAO ; Pei HUANG
Chinese Journal of Hospital Administration 2010;26(8):574-577
The ongoing health reform is bringing reforms of public hospitals under spotlight in China, attracting growing attention onto its progress and roadblocks. Probing into the "three steps" of public hospital system reform in Wuxi, this article analyzed such a reform in the city. It described the measures and initial outcomes gained in such fields as the hospital trusteeship reform, the separation of hospital regulation from its administration, and the hospital board system. Citing the case of Wuxi No. 2 People's hospital as an example, the authors introduced the innovative efforts made in hospital internal mechanism, management innovation, medical service model, hospital connotation construction, and talents cultivation.
10.Construction and identification of yeast cDNA expression library of human dermal papillae cells in primary culture
Zhiqiang SONG ; Lihua SUN ; Zhifang ZHAI ; Fei HAO
Chinese Journal of Dermatology 2010;43(6):408-410
Objective To construct yeast cDNA expression library of human dermal papillae cells (DPCs) in primary culture.Methods Human dermal papilla cells (DPCs) were isolated by two-step digestion method and cultured in DMEM medium.Total RNA was extracted from primary DPCs that exhibited an aggregative behavior in culture,then,cDNA was synthesized and amplified by using CloneMinerTM cDNA Library Construction kit to construct primary cDNA library and yeast cDNA expression libary.Results The average titer and total clones were 7.0×106 colony forming units(cfu)/ml and 1.4×107 cfu respectively in the primary library,5.5×106 cfu/ml and 1.1×107 cfu respectively in the yeast expression library.The average insert size Was 1.2 kb and the recombination rate was above 95%.Conclusions The yeast cDNA expression library of DPCs in primary culture has been successfully constructed.which will lay a foundation for screening proteins interacting with HSPC016 gene in DPCs with yeast two-hybrid system.