Objective To investigate the value of T-lymphocyte subsets CD3+ ,CD4+ and CD8+ percentage detection in the diag-nosis of early-onset neonatal sepsis .Methods The neonates born in January 2014 to June 2015 with suspected infection and onset within 7 d were included into this study .The venous blood was collected within 24 h after admission .CD3+ ,CD4+ ,CD8+ lympho-cyte percentage ,C reactive protein and blood routine were detected .The blood culture was performed before the use of antibacterial drugs .The full-term neonates with early onset sepsis confirmed by blood culture were taken as the sepsis group and those of nega-tive blood culture was taken as the local infection group select the blood culture negative for local infection group .At the same time , the hospitalized full-term neonates with hyperbilirubinemia (excluding cases caused by factors such as infection ) served as the con-trol group .The flow cytometery was adopted to detect the samples of above neonatal patients .The lymphocyte subsets CD3+ , CD4+ and CD8+ percentages were observed and compared among 3 groups .Results CD3+ [(40 .3 ± 10 .6)% ] ,CD4+ [(28 .6 ± 11 .2)% ] and CD8+ [(10 .8 ± 2 .6)% ] in the sepsis group were lower than CD3+ [(64 .8 ± 9 .8)% ] ,CD4+ [(48 .9 ± 10 .2)% ] , CD8+ [(17 .6 ± 5 .6)% ] in the local infection group and CD3+ [(62 .6 ± 11 .6)% ] ,CD4+ [(46 .4+13 .6)% ] and CD8+ [(16 .5 ± 7 .3)% ] in the non-infection group ,the differences were statistically significant (P<0 .05);CD3+ ,CD4+ and CD8+ had no statisti-cal difference between the local infection group and non-infection group(P>0 .05) .Conclusion CD3+ ,CD4+ ,CD8+ percentage can be used as an indicator for the diagnosis of early onset neonatal sepsis .

Objective To observe efficacy of Pulmicort in treatment of capillary bronchitis.Methods Children with capillary bronchitis were randomly divided into two groups,ninty-eight cases of study group were treated with Pulmicort,fifty-eight cases of control group were treated with common therapy,observe clinical symptom:cough,breathes heavily. Results Two groups efficiency were 93.8% and 72.4% respectively.The study group was significantly better than that in control group. Conclusion Pulmicort inhalation in treatment of children with capillary bronchitis are effective.

OBJECTIVE To apply and practice the process and effect of low temperature steam formaldehyde(LTSF) sterilization for heat-sensitive goods.METHODS The heat-sensitive medical instruments were sterilized in LTSF sterilizer under the appropriate media conditions,all procedures,including preparation,packing,sterilization,unloading,storage etc were strictly in the right way.RESULTS Sterile result has been guaranteed.CONCLUSIONS LTSF is a kind of effective sterilization method for low temperature sterilization application.By this way,the quality of sterile goods has been reached,the rate of nosocomial infection has been lowered,requirements of hospital clinical departments for sterile goods also have been met.

Objective To analyze the application effect of standardized colostomy irrigation nursing in rectal cancer patients with Miles operation.Methods 126 rectal cancer patients underwent Miles operation were randomly divided into the observation group and the control group,63 cases in each group.The patients of the control group were treated with traditional colostomy natural defecation ostomy care model,and the patients of the observation group were given standardized colostomy irrigation nursing mode.Before and after nursing,the life quality function score,life quality score and individual total care expenses of the two groups were compared.Results Before nursing,the function score and quality of life event scale score between the two groups were compared,and the differences were not statistically significant(all P >0.05).After nursing,physical function[(97.84 ±3.83)points],cognitive function[(96.79 ± 8.77)points],emotional function[(94.57 ±8.76)points],role function[(96.57 ±1.57)points],social function [(92.77 ±6.17)points],nausea and vomiting[(0.56 ±0.23)points],insomnia[(2.64 ±1.78)points],pain [(1.48 ±0.63)points],fatigue[(5.13 ±2.47)points],loss of appetite[(1.76 ±0.83)points]of the observation group were significantly better than those of the control group [(90.22 ±3.79)points,(80.99 ±8.74)points, (83.26 ±13.83)points,(78.85 ±3.26)points,(83.67 ±3.45)points,(1.27 ±0.76)points,(12.25 ±3.61)points, (4.08 ±1.67)points,(6.76 ±2.98)points,(3.47 ±1.62)points],there were statistically significant differences between the two groups(t =11.22,10.13,5.48,38.87,10.22,7.10,18.95,11.56,3.34,7.46,all P <0.05).After nursing,the product cost[(1 488.78 ±102.49)yuan],nursing cost[(158.46 ±10.73)yuan],total cost[(1 638.79 ± 106.73)yuan]of the observation group were significantly lower than those of the control group [(2 070.17 ± 141.78)yuan,(311.17 ±99.32)yuan,(2 380.63 ±212.79)yuan],the differences were statistically significant (t =26.38,12.13,24.73,all P <0.05 ).Conclusion Standardized colostomy irrigation nursing can improve overall quality of life in patients of rectal cancer after Miles operation,reduce the economic and psychological burden,and its application effect is better.

Objective To investigate the features of multiple pelvic injuries (fracture combined with multiple traumas) and their effects on management and prognosis. Methods A total of 233 cases of hemodynamically unstable pelvic fractures were grouped according to the patterns of violence, fracture and associated injuries. They were assessed with measured indexes in physiological state, anatomic injury and blood requirement. Their possibilities of survival (Ps) were also predicted. All the data were statistically analyzed. Results The pelvic fractures were often combined with multiple traumas and frequently resulted from high energy traffic accidents. The correlations among sources of bleeding, amounts of blood loss and hemodynamical changes were disproportional. In unstable pelvic fractures with extra pelvic bleeding, BP and blood requirement were significantly different, and their AIS and ISS of the pelvis were higher and their Ps was lower than in the other groups. Conclusions Multiple pelvic injuries caused by high energy are often highly risky and combined with multiple traumas and several sources of bleeding. A clear and precise assessment of the total injuries and sources of bleeding will help treat the patients with different managements.

AIM:To detect the effect and underlying mechanism of parthenolide ( PN) on neointimal hyperpla-sia.METHODS:After 1 week of high-fat feeding, 30 male New Zealand white rabbits (2.0~2.3 kg) were randomly di-vided into 6 groups: sham +NS, rabbits received 0.9% normal saline after sham operation; sham +DMSO, rabbits re-ceived DMSO after sham operation;balloon injury(BI)+NS, rabbits received NS after balloon injury;BI+DMSO, rabbits received DMSO after balloon injury;BI+PN low, rabbits received PN at 1 mg/kg after balloon injury;BI+PN high, rab-bits received PN at 2 mg/kg after balloon injury .The drugs were intraperitoneal injected once a day after the operation until sacrifice.After fed with high-fat diet for 4 weeks, the intima-media thickness, the expression of caspase-1, IL-1β, the lev-els of IL-8, TC, TG, LDL and HDL in the serum were measured .RESULTS:Compared with sham +DMSO group, the thickness of intima, the amount of caspase-1, IL-1βand IL-8 in BI +DMSO group were significantly increased ( P <0.05).The levels of caspase-1, IL-1βand IL-8 were significantly decreased in BI +PN high group compared with BI +DMSO group (P<0.05).CONCLUSION:Neointimal hyperplasia is suppressed by PN after balloon injury , the potential mechanism may be associated with its anti-inflammatory role .

Objective To explore the causes of bone fascia compartment syndrome after radial artery coronary artery intervention and sum up the nursing experience. Method The clinical data of 8 patients with bone fascia compartment syndrome after radial artery coronary artery intervention from January 2009 to December 2014 were analyzed retrospectively to summarize the nursing countermeasures, including close observation of illness, swelling and pain nursing, medication and blood and monitoring of coagulation functions. Result The forearm of all patients were painful, swollen and enlarged, 6 of them with radial pulse abating,1 with finger pulling pain, 2 with muscle decreasing. Conclusions The early observation and treatment of bone fascia compartment syndrome are critical. Great importance to the complaints of patients should be attached in view of the causes of complications so that effective nursing strategy can be taken to save time of conservative treatment, alleviate the patients'pains and promote their early recovery.

To explore the effect of Problem-based learning (PBL)in the clinical clerk-ship of internal medicine,we applied PBL method and traditional method from 2006. Compared with the traditional method,PBL method and traditional method is a better way to enhance students'ability of analyzing and solving problems.But there are some problems related to the PBL in pretent stage of medical education in our country.

In order to raise international comprehensive medical talents to have strong competition capability,the bilingual teaching is on the rise in the domain of medical education. Questionnaire-based survey among the 5-year-proguam medicinal undergraduate students was conducted to investigate the students'learning condition,acquaintanceship and requirements on the bilingual teaching so as to further reform and explore ways to improve the quality of bilingual teaching

OBJECTIVE:To compare the clinical effects of two isomates of glycyrrhizic acid on patients with hemorrhagic fever with renal syndrome(HFRS) METHODS:78 patients with HFRS were randomized into two groups:A and B Patients of group A and group B were given ?-glycyrrhizic acid(Diammonium Glycyrrhizinate) and ?-glycyrrhizic acid(Stronger Neo-Minophagen C) intravenously,respectively The clinical parameters such as rate of phase-skip,etc were observed and compared between two groups RESULTS:The rate of phase-skip in group B was higher than that in group A with significant difference(P