Objective] Summarizing the diagnosis and treatment about the tumor-related disease from “Synopsis of Prescriptions of the Golden Chamber”, thus to better use classic in clinic. [Method] Sort out the book that involves tumor-related diseases pathogenesis, therapeutic treatment and syndrome differentiation treatment, combine with each of the clinical words, analyze and summarize all the clinical experience of physicians. [Results]The book is not equipped with tumor article, many cancer-related clinical manifestations of the disease description can be seen in each chapter, although not forming a system, its pulse and symptoms are involved in different parts of the tumors. And the symptoms of lung cancer, stomach cancer, and gynecological tumors also have been mentioned.[Conclusion] Visible in the book, the etiology and pathogenesis of tumor related diseases have a certain understanding. In the aspect of treatment, pay attention to combining disease syndrome differentiation, treatment and syndrome differentiation. Then concluding all the physicians clinical experience we can see that “Synopsis of Prescriptions of the Golden Chamber”for the treatment of tumors has great significance. And classic of traditional Chinese medicine for modern TCM clinical still have important reference value and guiding significance.

Objective: The purpose of this study was to investigate the effect of TNF-? on osteoclast differentiation in primary murine bone marrow cell culture with and without RANKL. Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 ?g/L) with different concentrations of TNF-? (0, 1, 10, 100 ?g/L) for 5 days, the formation of TRAP(+) multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 ?g/L) and M-CSF (25 ?g/L) with or without 10 ?g/L TNF-? for 4, 5, 6 and 9 days. The number of TRAP(+) multinucleated cells and resorption pits on dentine slices were counted under light microscope. Results: In the absence of RANKL, TNF-? was unable to induce osteoclast formation from murine bone marrow precursors. In the presence of RANKL, TNF-? augmented osteoclastogenesis and bone resorption, and this effect occurred only on the early stage. Conclusion: TNF-? enhances RANKL-induced osteoclast formation and function, but can’t substitute for RANKL. TNF-? stimulates osteoclast differentiation, but not survival.

Objective To analyse subtyping of iscbemic stroke by the criteria of Trial of Org 10 172 in Acute Stroke Treatment (TOAST), its distribution, and relationship between TOAST subtypes, its risk factors and recurrence. Methods Clinical data of 441 patients of ischemic stroke were collected prospectively during October 1, 2006 to September 30, 2007. All the patients were classified by TOAST criteria into five major subtypes and followed-up for one year since its first episode. Dates of its recurrence and death due to recurrence were recorded. Results All the 441 patients with ischemic stroke could be subtyped etiologically according to TOAST criteria as follows: etiology undetermined in 42.3 percent, small-vessel occlusion in 30.9 percent, large-artery atherosclerosis in 17.3 percent, cardioembolism in 9.3 percent, and others in 0.2 percent, without statistically significant difference in its gender and age distributions. Recurrence rate of iscbemic stroke in one year since its first episode for varied subtypes was 33.3 percent for cardioembolism, 14. 6 percent for etiology undetermined, 13.7 percent for large-artery atherosclerosis and 6.9 percent for small-vessel occlusion, respectively. There was no significant difference in risk factors for varied subtypes of iscbemic stroke, including history of smoking, hypertension, diabetes, cardiac disease, and serum levels of total cholesterol, low-density lipoprotein cholesterol, fihrinogen and glucose. Conclusions Varied subtypes of iscbemic stroke had different risk factors and recurrence rate by TOAST criteria, which can be used as an etiologic classification for its secondary prevention.

Objective: To establish the permanent tooth germ missing animal model for future research on the root resorption of deciduous tooth in the absence of permanent tooth germ. Methods: The permanent tooth germ missing animal model was established by surgical removal of the permanent tooth buds in a male 11-week-old Beagle dog. Root resorption of the deciduous teeth without permanent successors was observed by taking periapical films periodically,and compared with physiological root resorption. Once the sign of root resorption of the deciduous teeth without permanent successors was detected on radiographic films, the animal was sacrificed and the mandibular bone was collected for histological study. Results: Root resorption of the deciduous teeth with the presence of permanent tooth germ started at 20 weeks after birth, while root resorption of deciduous teeth without permanent tooth germ started 26-27 weeks which was significantly delayed. Histological studies showed that a large number of multinucleated giant cells were present on the pulpal surface of the root, while only few of them were seen on the outer surface. Conclusion: The permanent tooth germ missing animal model was successfully established in this study which simulated the case of congenital absence of permanent tooth germ in human. Root resorption of deciduous tooth without permanent tooth germ was significantly delayed than the deciduous tooth with permanent tooth germ.

Objective: To develop titanium posted keeper removable magnetic attachment and to decrease its influence on magnetic resonance imaging(MRI). Methods: Pre fabricated soft magnetic alloy pin cap keeper(PSAPK), casting non precious alloycoping keeper(CNPAK), casting precious alloy coping keeper(CPACK), titanium post keeper(TPK) and titanium post(TP) were prepared and applied on the maxilla of a volunteer, MRI was taken and measured. Results: ①The attachments producing artifacts in MRI from the biggest to the smallest were listed in following rank: PSAPK, CNPAK, CPACK, TPK and TP. ②After removing the keeper from titanium post keeper, the post had no artifact on MRI. ③The artifact was the smallest taken with SE sequence. Conclusion: Titanium post keeper is an effective way to solve the artifact problem and will bring benefits to the patients who need MRI examination of head.

To review the research of block copolymer micelles as delivery system for poorly soluble(antineoplastic) carrier over the last 10 years,including the composition,preparation methods and factors which influence the loading efficiency,physicochemical properties,and targeting characteristics of block copolymer micelles.Block copolymer micelles are self-assembled structures formed from amphiphilic block copolymers by dispersing in aqueous media.The hydrophilic blocks of the copolymer form the outer shell of the micelle,while the hydrophobic blocks form the inner core,and the proper coreshell micellar architecture was constituted.Block copolymer micelles have a whole set of unique properties,such as small sizes,narrow particle size distribution,high drug loading capacities,and available disposition characteristics in the body.Block copolymer micelles have been found as promising drug carriers due to making poorly soluble antineoplastic lysis,toxicities and side effects decrease,bioavailability increase,and targeting the drugs to specific sites in a passive way or attaching ligands in an active way,which can be specifically(recognized) by receptors onto the surface of copolymers.

Objective:To investigate the effect of TGF-? on osteoclast differentiation in primary murine bone marrow cell culture in the presence of receptor activator of NF-?B Ligand(RANKL) and macrophage colony stimulating facotr(M-CSF).Methods:M-CSF-dependent bone marrow cells were isolated from 5~6 weeks old mice,and cultured in the presence of RANKL at 30 ng/ml and M-CSF at 25 ng/ml with or without 1 ng/ml of TGF-? for 4 and 9 days respectively.TRAP positive multinucleated cells and resorption pits on dentine slices were counted under light microscope.Results:TRAP positive multinucleated cells were induced from murine bone marrow cell cultures with both RANKL and M-CSF,and these cells formed resorption pits on dentine slices.The number of TRAP positive multinucleated cells and resorption pits were significantly increased by 1 ng/ml of TGF-?(P

Objective To explore the siqnificance of event related potential(ERP) N400 on stroke patients with aphasia and provide theoretical basis for clinical operations.Methods 29 stroke patients with aphasia(aphasia group) and 58 stroke patients without aphasia(control group) were selected,which were examined with N400.The latency and amplitude of N400 were compared between the two groups.Results The difference waves' latency and amplitude of aphasia group were longer and higher than those of control group.The difference had statistical significance (all P ＜ 0.05).Conclusion N400 is a significant marker for the language disturbance of aphasia patients.

In order to understand the academic attitude of medical postgraduates,a questionnaire survey was conducted among full-time postgraduate students.The impacts of working experience,gender,marriage status and source on the academic attitude were analyzed,and the improved suggestion and presumption for academic attitude were provided.

Objective: To explore suitable concentration of recombinant human transforming growth factor β1 (rhTGF-β1) usage and study the effect of rhTGF-β1 on differentiation of dental pulp stem cells (DPSCs).Methods: DPSCs were isolated from the undiseased third molars of people aged 18-25 years and cultured according to instructions in vitro.Different concentrations (1 , 6 , 10 μg/L) of rhTGF-β1 were added to the culture medium to examine DPSCs proliferation by CCK-8 (cell counting kit-8) assay.The suitable concentration was then selected.For differentiation, the DPSCs were incubated for 7 or 14 days with rhTGF-β1 supplemented with osteo/odontoblastic induction medium containing 10 nmol/L dexamethasone, 10 mmol/L b-glycerophosphate, 50 g/L ascorbate phosphate, 10 nmol/L 1,25-dihydroxyvitamin D3 and 10% fetal bovine serum.The cells were then washed 3 times with phosphate-buffered saline and sonicated with 1%Triton X-100 for 30 minutes on ice.Cellular alkaline phosphatase (ALP) activity was assayed with p-nitrophenyl phosphate as the substrate.The enzyme activity was expressed as p-nitrophenyl produced per milligram of protein [bicinchoninic acid (BCA) protein assay kit].To examine mineral nodule formation, the cultured cells were fixed in 4% paraformaldehyde and washed in water, and the mineralization of the extracellular matrix was assayed by 1% alizarin red S staining and elution of staining was examined as optical density (D) under microplate reader.The mean difference was considered significant at 0.05 and 95% confidence interval.Results: The DPSCs had ty-pical fibroblast morphology and could form mineral nodules after being cultured with osteo/odontoblstic induction medium for 14 days.6 μg/L rhTGF-β1 significantly promoted the DPSCs proliferation on the 3rd and 5th days.After the incubation of osteo/odontoblastic induction medium, the DPSCs with the 6 μg/L rhTGF-β1 increased ALP activities compared with the control;D values in the 6 μg/L rhTGF-β1 group was 0.31±0.03, while the control group was 0.02±0.01(P<0.05).The total protein content in the 6 μg/L rhTGF-β1 group was (2 775.46±83.54) mg/L, and the control group was (1 432.20±110.83) mg/L (P<0.05).To eliminate the cells proliferation influence, relative ALP activities, which was defined as the total ALP divided by the total protein content, the 6μg/L rhTGF-β1 group was 6 times higher than the control group.Alizarin red S staining showed increased mineral nodule formation in the rhTGF-β1 group.The elution of staining under microplate reader also showed more optical density in the 6 μg/L rhTGF-β1-treated cells (0.83±0.02) than that in the control groups (0.55±0.05, P<0.05).Conclusion: 6 μg/L rhTGF-β1 could significantly promote DPSCs proliferation and odontoblastic differentiation in vitro.