The etiology and pathogenesis of Crohn’s disease(CD)are not fully clear,and genetic susceptibility, immunologic disorder,intestinal barrier dysfunction and intestinal microecology are considered to be involved in the pathogenic mechanism of CD. In recent years,the relationship between intestinal microecology and CD has received much attention. Several studies confirmed that the intestinal microecology in CD patients was different from that in normal person. The change of intestinal microecology was correlated with the occurrence of CD,and modulation of intestinal flora was effective in the treatment of CD. This article reviewed the relationship between intestinal microecology and CD and the therapeutic prospect of intestinal microecology for the treatment of CD.

Objective Through observing the changes of serum creatinine (Scr),blood urea nitrogen (BUN),and Cystatin C (Cys C) to prove whether the treatment of patients of diabetic nephropathy (DN) in V period with Jiawei-qidan-tangshen decoction is really effective.Methods 60 patients of DN in V period were randomly divided into a treatment group and a control group by random number table method,with 30 patients in each group.Glycated hemoglobin (HbA1c),serum creatinine (Scr),blood urea nitrogen (BUN),and Cystatin C (Cys C) were detected before and after the treatment.Results 12 weeks after treatment,Scr,Cys C (19.19±37.06) μmol/L,(1.81 ±0.69)mg/L had significantly declined in the treatment group (P＜0.001),and had no significant change (147.25±62.70)μmol/L,(2.04± 1.20)mg/L in the control group (P＞0.05).Total effective rate of Scr and Cys C was 73.33％ and 83.33％ in the treatment group and 40％ and 53.33％ in the control group respectively,both showing significant difference (P＜0.05).Conclusion It is effective to treat patients of DN in V period with,Jiawei-qidan-tangshen decoction,and have no obvious side effects.

Objective To study the influence of different starting time of hyperbaric oxygen(HBO) therapy on severe brain injury patients.Methods 60 cases of acute cerebral infarction patients were divided into 2 groups,namel 48 hours to 7 days group(group A) and 8 days to 15 days group(group B) after injury.Fugl-Meyer assemment and modified Barthel index were employed to assess the patients before and after HBO for 30 days in the 2 groups.Results After 30 days of HBO therapy the Fug-Meyer assemment and modified Barthel index in the A group were better than those of B group,there were significant differences between the 2 groups(P

Objective:To investigate the effect of basic fibroblast growth factor(bFGF) on the proliferation of gingiva-derived mesenchymal stem cells(GMSCs) in vitro.Methods:GMSCs were isolated from healthy gingival tissue samples and identified.GMSCs of passage 4 were treated by bFGF at 0,0.5,1,5,10,20 ng/ml respectively for 1-9 d.The proliferation of the cells was evaluated using CCK-8 kit.Results:bFGF at 0.5-20 ng/ml increased GMSCs proliferation.0.5-10 ng/ml of bFGF showed dose and time dependant proliferation promoting effect on GMSCs.Conclusion:bFGF can increase GMSCs proliferation ability in a dose and time dependant manner.

Objective To compare the detection results and estimate the bias of two hematocyte analyzers of different brands . Methods Sysmex XE‐2100 hematocyte analyzer and Abbott CD‐1700 hematocyte analyzer were chosen to be the reference instru‐ment and comparison instrument ,respectively .40 cases of fresh whole blood samples were collected for the detection of WBC ,RBC , HGB ,HCT ,MCV ,MCH ,MCHC ,and PLT respectively by the two instruments .The correlation coefficient (r) ,regression equa‐tions and bias were calculated and compared to determine the comparability of the two instruments .Results The precisions of two hematocyte analyzers were satisfactory .When fresh whole blood was used as calibrators ,the results of all eight items of the two in‐struments had good correlations (r>0 .975) ,and the relative bias was acceptable .Conclusion The results of two hematocyte ana‐lyzers are comparable .With fresh whole blood using in the comparison test between different hematocyte analyzers ,systematic er‐rors can be discovered in time .

0.05).There was correlation between the expression of 5-LOX and TNM stages.The expression of 5-LOX in stages III~IV of pancreatic cancer was markedly higher than that in stages I~II of pancreatic cancer(P

A method for the determination of caffeine in urea was developed based on bubble-in-drop single drop microextraction (BID-SDME) followed by gas chromatography/mass spectrometry (GC-MS).Under the optimum conditions including chloroform as extraction solvent, an exposure volume of 1 μL, a bubble volume of 1.6 μL, stirring for 5 min at 300 r/min, 15% (m/V) NaCl, and a distance of 1 cm between bubble and stirring bar, the detection limit of this method was as low as 0.003 mg/L and the linear range was from 0.005 mg/L to 10 mg/L with correlation coefficient of 0.982.The recoveries of caffeine were from 89.2% to 107.5% at different spiked levels in human urine and the relative standard deviation (RSD, n=6) was less than 8%.

0.05).Sexual dysfunction was 13.8% and 46.5% respectively,and was significant(P

Objective To investigate the expression of 5-LOX in pancreatic cancer tissue and the relationship between 5-LOX expression and expression of VEGF.Methods The expression of 5-LOXmRNA,(VEGFmRNA) in 35 pancreatic cancer fresh tissue samples were detected by semi-quantitive reverse(transcriptase)-polymerase chain reaction method.Results Expression of 5-LOXmRNA,VEGFmRNA in(pancreatic) cancer tissue were 74.3%,60% respectively,and the expression was correlated to the with(clinical) stages of the tumor;also expression of VEGFmRNA was correlated to the differentiation of the tumor.Expression of 5-LOXmRNA and VEGFmRNA were synergetic in pancreatic cancer(P

Objective To investigate the value of CT virtual endoscopy(CTVE) and multiple plane(reconstruction)(MPR)for preoperative staging of rectal cancer.Methods Forty-five patients with rectal(cancer) were examined by CTVE and MPR to determine the preoperative staging,and the reselts were compared with CT.Results For evaluation of intramural invasion,the accuracy rate of CTVE and MPR was 86.7%(39/45),pelvic CT was 66.7%(30/45)(P