[Objective] To sum up pro. Lu Binjiu's unique thought and experience in treating alcoholic liver disease with Fructus Broussonetiae and Turnju-jube in coordination. [Method] By introducing the functional features of Fructus Broussonetiae and Turnjujube and TCM's recognition to the disease, it introduces 2 recipes summing up his scholar thought and experience in treating the said disease with the 2 herbs. [Result] The disease is caused by wine in-juring spleen and stomach, inducing wet hotness accumulated inside, later on the Qi, blood and water functioning one another, final y forming weak body resistance and pathogeny lingering;the Fructus Broussonetiae can nourish kidney and clear liver, help kidney for diuresis;and Turnjujube can induce diure-sis to al eviate edema and is good at alcoholic toxin;so pro. Lu uses both the herbs for the disease. [Conclusion] Pro. Lu's method has definite cure effect on the disease above, worthy our learning, reference, inheritance and promotion.

Objective To investigate the effects of ABO blood group factors on postoperative cognitive function in elderly patients.Methods One hundred twenty patients, aged 65-75 yr, with body mass index of 17-26 kg/m2, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ , scheduled for elective thoracic surgery, were divided into 4 groups (n =30 each) according to the blood group: blood group A group (group A), blood group B group (group B), blood group O group (group O) and blood group AB group (group AB).Combined general-epidural anesthesia was used during surgery, patient-controlled epidural analgesia was performed after surgery, and visual analogue scale score was maintained ≤4.Before surgery (T0), and at 6, 24 and 72 h after surgery (T1-3), Mini-Mental State Examination (MMSE) was used to assess the cognitive function, and the occurrence of postoperative cognitive dysfunction (POCD) was recorded.Blood samples were drawn from the central vein at T0-3 for determination of neuron-specific enolase (NSE) and S-100β protein concentrations in serum.Results Compared with the values at T0, MMSE scores were significantly decreased, and serum NSE and S-100β concentrations were increased at T1-3 in A, B and AB groups, and at T1 and T2 in group O (P＜0.05).Compared with group A, MMSE scores were significantly increased, and serum NSE and S-100β concentrations were decreased at T1-3 in B, AB and O groups (P＜0.05).Compared with group O, MMSE scores were significantly decreased, and serum NSE and S-100β concentrations were increased at T1.3 in B and AB groups (P＜0.05).There was no significant difference in MMSE scores and serum NSE and S-100β concentrations at T1-3 between group B and group AB (P＞0.05).The incidence of POCD from the high to the low was group A, group AB, group B, and group O in turn (P＜0.05 or 0.01).Conclusion ABO blood group factors affect postoperative cognitive function in elderly patients, elderly patients of blood group A are found to be prone to develop POCD, and the incidence of POCD is relatively low for elderly patients of blood group O.

BACKGROUND:Previous studies have confirmed that as an exogenous origin of endometrial epithelial cel s, bone marrow stem cel s are involved in the functional reconstruction of the injured endometrium. OBJECTIVE:To discuss whether bone marrow mesenchymal stem cel s can differentiate into endometrial epithelial cel s in vitro. METHODS:Passage 2 bone marrow mesenchymal stem cel s and endometrial stromal cel s were col ected and subjected to different treatments:bone marrow mesenchymal stem cel s were cultured alone in the DMEM/F12 medium containing 2%fetal bovine serum or in the DMEM/F12 medium containing 2%fetal bovine serum, 10-7 mol/Lβ-estradiol and 10μg/L epidermal growth factor as groups 1 and 2, respectively;bone marrow mesenchymal stem cel s co-cultured with endometrial stromal cel s by Transwel chamber were cultured in the DMEM/F12 medium containing 2%fetal bovine serum or in the DMEM/F12 medium containing 2%fetal bovine serum, 10-7 mol/Lβ-estradiol and 10μg/L epidermal growth factor as groups 3 and 4, respectively. After 5 days culture, bone marrow mesenchymal stem cel s at the bottom of the culture plate were col ected to detect expressions of epithelial cel markers, such as CK7, CK18, CK1 and EMA by RT-PCR technology, and to determine keratin expression using immunofluorescence assay. Besides, expressions of these epithelial cel markers in the group 3 were detected using RT-PCR at 1, 3, 5 and 7 days of culture, respectively. RESULTS AND CONCLUSION:mRNA expression of CK7 showed a significant successive rise in the groups 1, 2, 3, 4 and it was highest in the group 4. mRNA expressions of CK18 and CKl9 in the later three groups had no significant differences, but al significantly higher than those in the group 1. Compared with the groups 1 and 3, mRNA expression of EMA in the groups 2, 4 were significantly higher, but no significant differences existed between groups. Expression of keratin was strongly positive in the group 4, weakly positive in the group 3 and negative in the groups 1, 2, respectively. Furthermore, with the increase of culture time, mRNA expression of CK7 exhibited a constant increase, which was significantly higher at 5 and 7 days than at 1 and 3 days;but there were no significant differences in expressions of CKl9, CKl8 and EMA at different time points. These results show that bone marrow mesenchymal stem cel s can differentiate into endometrial epithelial cel s in vitro under certain conditions. Moreover, it can remarkably promote the endometrial differentiation under the combined effects of some exogenous and endogenous factors.

Infectious mononucleosis(IM) was one of the clinical common diseases, caused by epstein-barr virus infection in more than 90%patients. Changes of the surface antigen of the B cells with EB virus infection caused strong immune response of T lymphocytes, besides, helper T lymphocyte, dendritic cells, NK cells also played an important role in the pathogenesis of IM. The incidence of IM was not only closely related with social environment in childhood, but also closely with the number of siblings, birth order, and sunshine factor. In addition to the typical clinical manifestations, IM also can cause a variety of complications, such as spleen rupture and necrotizing mediastinitis , and could be life-threatening. Auxiliary examination means such as state of peripheral blood lymphocyte apoptosis, plasma proteomics, cerebrospinal fluid analysis, load test of EB virus can help to diagnose. Application of antiviral drugs was wider, but hormone was still controversial. Vaccine research progress had been slow, but there were vaccines for human test, which will provide effective measures for the prevention of IM.

Bronchiolitis ; diagnosis ; Haemophilus Infections ; diagnosis ; Humans ; Silicosis ; diagnosis

Objective To compare the body weight,height,head circumference of premature babies fed by different ways,and to analyze the advantages and disadvantages.Methods 260 premature infants were selected as the research subjects,according to random number table,they were divided into four groups.Group A:premature infant formula feeding,80 cases;Group B:pure breast feeding group,60 cases;Group C:normal full term formula feeding,60 cases;Group D:premature infant formula mixed breast feeding,60 cases.All children were fed at the first 12 to 24h after birth.The body weight,height,head circumference and catch up full term time were evaluation index. Results After correct gestational age 40 weeks,group B children'weight,height,head circumference were higher than other groups,group C was the lowest levels,the difference was statistically significant (F =3.563,P <0.05).Correct gestational age 1,3,6 months later,group A children'weight,height and head circumference index were higher than other feeding group,the level of group D was the second,the lowest level was group C,the differences were statistically significant (F =3.011,2.853,2.779,all P <0.05).In 6 months,group A,38.8% (31 /80),was the fastest one to reach the full term infant,the difference was statistically significant (χ2 =29.149,P <0.05).At other time,the pur-suing number of group A was bigger,but there was no statistically significant difference.Conclusion For premature babies,preterm infant formula milk powder or premature infant formula and milk feeding way is better than that of pure breast feeding and normal full term milk powder,which can guarantee the children's nutrition demand,shorten the time of pursuing full term infant,promote baby's health.

Objective: To study the role of adrenomedullin(ADM) in normal early pregnancy. Methods: Plasma concentrations of ADM were measured in 30 normal early pregnancy and 10 non-pregnant women by radioimmunoassay. The expression of ADM?ADM mRNA and ADMR mRNA in villus of normal early pregnancy were determined by immunohistochemistry and in situ hybridization respectively. Results: The plasma concentration of ADM in normal early pregnancy was significantly higher than that of normal non-pregnant women (P

Two 78-years old-old man and woman presented with thyroid tumor without a past history of chronic thyroiditis. Physical examinations showed touchable lumps in thyroid without other lymph nodes swelling or extra nodal lesions. Two cases both had thyroid function test, color Doppler ultrasound examination, computed tomography (CT) scan and fine needle aspiration (FNA) before surgery. The patients underwent partial thyroidectomy or subtotal thyroidectomy. Postoperative pathologic findings were consistent with malignant lymphoma. The diagnosis mainly relied on the postoperative pathologic findings.
Aged ; Female ; Humans ; Lymphoma ; Male ; Thyroid Neoplasms

Objective To investigate the efficacy of ultrashort and short-acting insulin after subcutaneous insulin infusion pump under glucose monitoring in treatment of diabetes.Methods 110 cases with type 2 diabetes were selected,they were randomly divided into two groups with a random number table:the study group and the control group,55 cases in each group.The study group was treated with short -acting insulin after subcutaneous injection of insulin pump,the control group was treated with short -acting subcutaneous insulin infusion by insulin pump therapy, glucose monitoring system was used to monitor glucose during treatment of the two groups.The clinical efficacy after a month of treatment was observed.Results Before treatment,fasting blood glucose(FPG),postprandial 2 h blood glucose(2h PG),bedtime blood glucose of the two groups had no statistically significant differences.After treatment, FPG and 2hPG,bedtime blood glucose in the study group were (6.01 ±1.06)mmol/L,(7.90 ±0.50)mmol/L, (7.13 ±0.95)mmol/L respectively,which of the control group were (5.98 ±1.04)mmol/L,(7.91 ±0.48)mmol/L, (7.14 ±0.94)mmol/L,which were significantly lower than before treatment (t =9.843,23.669,13.569,9.683, 22.881,13.710,P <0.05),the two groups after treatment showed no significant differences(P >0.05).The blood glucose time and insulin dosage in the study group were (5.59 ±1.30)d,(39.98 ±11.01)u/d,which of the control group were (6.98 ±1.40)d,(51.89 ±11.97)u/d,and there were significant differences between the two groups(t =1.825,5.431,P <0.05).The mean blood glucose,blood glucose standard deviation,time percentage of blood glucose≥10 mmol/L,time percentage of blood glucose≤3.9 mmol/L in the study group were (5.95 ±0.61 )mmol/L, (1.28 ±0.25)mmol/L,(14.16 ±2.13)%,(6.35 ±1.45)%,which in the control group were (6.59 ±0.94)mmol/L, (1.54 ±0.29)mmol/L,(15.15 ±2.14)%,(7.96 ±1.42)% respectively,and the differences were statistically significant between the two groups(t =4.236,503.6,2.432,5.883,P <0.05).Conclusion Glucose monitoring system in monitoring blood glucose is accurate and reliable,ultra -short -acting insulin after subcutaneous insulin pump infusion in treatment of diabetes is better than short -acting insulin.

Objective This study was designed to investigate the expression and clinical significance of transcription factor E2F-1 and apoptosis-off gene Bcl-2 in human colorectal carcinoma.Methods Immunohistochemistry is used to detect the expression of E2F-1、Bcl-2 proteins in 60 cases of human colorectal carcinoma tissues and their corresponding normal mucosa.Results The positive expression rate of E2F in cancer tissues is 56.7%,while the positive expression rate of E2F in corresponding normal mucosa is 6.7%,so the comparison between two tissues is significant (P