Objective To establish an HPLC method to determine the two principal constituent-capsaicin and dihydrocapsaicin in general alkaloids of Capsaicin.Method The analysis was performed on C18 column(4.6 mm?250 mm,5 ?m),with methanol-water(70:30) as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was set at 280 nm.Result The linear relationships of capsaicin and dihydrocapsaicin were within the range of 1.955～7.820 ?g(r=0.999 9) and 2.709～7.224 ?g(r= 0.995 5),respectively.The average recoveries were 101.57% and 108.87%,respectively.Conclusion The method is convenient,the result is accurate and reliable,and can be used to determine the content of two principal constituent in general alkaloids of Capsaicin.

目的观察神经阻滞治疗颈源性头痛的效果。方法对颈源性头痛患者30例行星状神经节阻滞,伴颈椎旁神经或耳颞神经阻滞治疗,观察治疗前后疼痛视觉模拟评分(VAS)、每周头痛发作次数、服用止痛药物与睡眠改善例数。结果治疗前后VAS评分改善(P<0.05),治疗后头痛完全缓解15例,好转9例,有效4例,无效2例;治疗后头痛发作次数及持续时间均缩短(P<0.05);治疗前服用止痛药物28例,治疗后2周12例,4周4例(P<0.05);睡眠不佳治疗前29例,治疗后2周13例,4周为4例(P<0.05)。结论神经阻滞是治疗颈源性头痛是一种良好的治疗方法。

Behavior Science pays more attention to humanity,and regards the management of people as the key factor of management.Nursing management is the process targeting at the progress of nursing quality and work efficiency.Under the new circumstance,it is very important for the head nurse to supervise the nurses with a scientific management conception in the hospital howadays which will encourage the nurses to fully exert their abilities. and help to improve the nursing quality finally.

Objective:To construct a suppression subtractive library of suppression-related genes from c serotype Streptococcus mutans (S.mutans). Methods:After being isolated from virulent and avirulent strain of S. mutans respectively, the intact and high-pure genomic DNAs were digested with restriction enzyme AluⅠ. The digested DNA of the avirulent strain ligated with an adaptor was used as tester DNA, and that of the virulent strain as driver DNA. Then the suppression subtractive hybridization(SSH) was carried out, the efficiency of ligation and subtraction were detected respectively. The subtracted fragments were inserted into vector pCR2.1 using T/A cloning kit and transformed into E. coli TOP10F' competent cells. The white colonies were selected to construct the suppression subtractive library. Results: Through electrophoresis of AluⅠ-digested DNAs, a smear ranged from 0.1 to 2.0 kb was observed. The ligation efficiency of tester DNA with adaptor was at least higher than 25 percent. The subtraction efficiency confirmed the success in enrichment of differential genes between virulent and avirulent strain of S. mutans. In the subtracted group, the appearance time of the 23S rRNA gene in both tester and driver DNA was later than that in the unsubtracted group by twelve cycles. It suggested that suppression subtractive hybridization happened indeed. Then the subtracted fragments were cloned and the suppression-related gene library between virulent and avirulent strain of S. mutans was constructed. Conclusions:The suppression subtracted library of suppression-related genes has been constructed.

Objective:To explore avirulent strain-specific new genes or new functions of already known genes from Streptococcus mutans of serotype c.Methods:Twenty-six DNA fragments unique to avirulent strain of Streptococcus mutans were sequenced.The sequences of these presumptive avirulence DNA fragments were subjected to homology search through BLASTn and BLASTx in public database,and their putative biological functions were analyzed.Results:The size of the DNA fragments ranged from 113 bp to 776 bp.The average G+C content was 38.27%,similar to that of the gene-codingsequences in Streptococcus mutans strain UA159 whose genome sequences were just completed.Seven clones were picked repeatedly.Of the nineteen DNA fragments,eight potentially represented new DNA fragments were registered in GenBank.The remaining DNA fragments showed high homology to known genes of Streptococcus mutans strain UA159.Conclusions: The gene analysis and identification supply the groundwork for further study of gene functions of the avirulent strain of Streptococcus mutans serotype c.

To achieve the goal of continuous improvement, measures of multi-link management were adopted for antimicrobial use, including establishing and improving organizational frameworks and rules and regulations, conducting various forms of training for all workers and staff, formulating criteria that were based on the frontier theories and could be easily applied, taking effective measures of intervention, using scientific evaluation methods, and getting timely feedback. The measures have proved to be effective in practice.

ObjectiveTo observe the effects of local mild hypothermia at different time on dopamine (DA) and 5-hydroxytryptamine (5-HT) concentration in rabbits brain basal ganglion with bilirubin encephalopathy. MethodsThe model of bilirubin encephalopathy was established, and treated with mild hypothermia for different time. The DA and 5-HT in the basal ganglion were detected with fluorescence spectrophotometry. ResultsThe DA and the 5-HT decreased in the basal ganglion significantly after modeling (P<0.01), and increased when treated for 6 h, 12 h or 24 h (P<0.01). ConclusionLocal mild hypothermia can significantly reduce the disorder of DA and 5-HT during bilirubin encephalopathy. The longer the hypothermia, the better the effect is.

Bcl-2 gene is the human homologous gene of anti-apoptotic gene Ced-9 in c-elegans, which can participate in regulations of cells apoptosis including suppression of neuronal apoptosis in cerebral ischemic penumbra.This review is about Bcl-2 anti-ischemic neuron injury, its possible mechanisms and the effects of anti-ischemic drugs on Bcl-2.

Ultra high performance liquid chromatography coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometry ( UPLC-MS/MS) and multivariate statistical analysis was applied to metabonomic
study of drug-induced liver injury. This investigation focused on the liver injury in clinic which was induced by antitumor drug, traditional Chinese medicine, statins and antibiotics. After precipitated by cold acetonitrile, the serum samples were separated on a HSS T3-C18 column using water (containning 0. 01% formic acid) and acetonitrile as mobile phase by gradient elution. Based on metabonomic profiles analysis of the orthogonal projections to latent structures discriminant analysis ( OPLS-DA ) and partial least squares-discriminant analysis ( PLS-DA) , 114 endogenous metabolites had been found to have significant differences between drug-induced liver injury patients and healthy volunteers, 38 endogenous metabolites have significant difference (p<0. 001). According to the results of the OPLS-DA, MS/MS data and human metabolome database ( HMDB) , phenylalanine and dimethylguanosine had been identified as potential biomarkers related to drug-induced liver injury for early discovery and diagnosis.

Biochemistry is a very practice-oriented courses,but the experiment has high cost,long cycle characteristics,and thus help ease the creation of virtual laboratory experiments and the conflict between teaching resources and the demand.We rely on our campus network to build a virtual biochemistry laboratory,with a view to promoting the biochemistry course construction and reforming the teaching methods