Objective: To determine the changes of fatty acids composition of eel oil after refining processes. Methods: The composition of fatty acids was determined by GC-MS. Results: The purity of eel oil was increased and the content of high-unsaturated fatty acids was not influenced after deodorization by water vapor distillation at 180 ℃ and 0.01 MPa. Its nutritive value was increased, while its purity and content of unsaturated fatty acids were further increased after winterization at 10 ℃. Its quality, especially unsaturated fatty acids, distinctly decreased after water vapor distillation at 227 ℃ and 0.01 MPa.Conclusion: The nutritive value of eel oil is increased after refining processes of water vapor distillation at 180 ℃ and 0.01 MPa, and winterization at 10 ℃.

Objective To observe the therapeutic effect of resveratrol (Rsv) on psoriasis like skin damage induced by imiquimod (IMQ) and study its mechanism.Methods BALB/c mice were randomly divided into four groups:control group,Rsv group,model (IMQ) group and Rsv treatment (IMQ + Rsv) group with six mice in each group.Psoriasis like skin model induced by smearing IMQ in the mice's ear for 8 d,20 mg Rsv cream were gave at the same time for treatment.Observe erythema and psoriasis degree,and measure ear thickness with vemier caliper.HE staining was used to observe tissue change,real time quantitative PCR (qRT-PCR) was used to detect K17,IL-17A,IL-19 and IL-23 mRNA and Western blotting were used to detected K17 protein expression in tissues.Results Compared with the model group,mice erythema area and the thickness of epidermal scale decreased significantly in Rsv treatment group;HE staining results showed that,Rsv significantly reduced ear marginal tissue epidermal thickness,and diminished severity of the psoriasis-like skin inflammation.qRT-PCR and Western blotting confirmed a Rsv dependent decrease of in K17,IL-17A and IL-19 in mRNA level and protein levels of K17.Conclusion Rsv improves psoriasis like skin damage by down-regulating expression of Keratinl 7.

Aim To investigate the effects of isoliquiri-tigenin(ISL)on C6 glioma cell proliferation and differ-entiation.Methods C6 glioma cells’viability and proliferation were respectively measured by SRB test. Colony formation of C6 glioma cells from different groups was assayed.After culturing the cells from each group,giemsa staining was used to observe cell mor-phology.RT-PCR was applied to detect mRNA expres-sion of GFAP.Western blot was applied to detect the expression of GFAP.Results ISL effectively inhibited the viability of C6 glioma cells when compared with the control group in a concentration-dependent manner (P<0.01).The morphological observation under light mi-croscope showed that:in the control group,most of the undifferentiated C6 cells showed long fusiform and po-lygonal shape.Compared to the control group,the C6 cells treated with ISL revealed alteration in morphology such as astrocytes with smaller smooth,round body and much finer longer,tapering processes.The cloning for-mation rate detection revealed that:the colonies in the control group semerged earlier and were larger than those experimental ones,the cloning formation rate was higher,while almost no effective cells colony emerged in ISL treated groups(P <0.01 ).Western blot and RT-PCR analysis showed that GFAP expression in the ex-perimental groups increased(P <0.01).Conclusion ISL may inhibit the proliferation of C6 glioma cells and induce their differentiation.

Objective To evaluate the risk factors for development of primary hepatic carcinoma(PHC)in patients with hepatitis B virus-related liver cirrhosis.Methods The cases of hepatitis B virus-related liver cirrhosis combined with PHC and hepatitis B virus-related liver cirrhosis alone were retrospectively analyzed in the Second Affiliated Hospital of Nanchang University.A total of 151 patients with hepatitis B virus-related liver cirrhosis combined with PHC were included in observation group and 136 patients with hepatitis B virus-related liver cirrhosis alone were included in control group.We have down the analysis of risk factors by χ2 test,t test and Logistic regression model.Results The clinical data of the two groups of patients showed statistically significant differences in six indicators,including gender,hepatitis B virus e antibodies(Anti-HBe),liver function grading(Child-Pugh),alpha-fetoprotein(AFP),blood glucose,and blood type,through univariate analysis(P<0.05).The results of multivariate unconditional Logistic regression analysis showed that AFP(OR=5.046,2.716,and 9.373,P<0.01)and Anti-HBe positive(OR=2.305,1.075,and 4.945,P<0.01)were risk factors for primary liver cancer.Conclusion Male shows a more possibility of occurrence of PHC than female.The positive Anti-HBe is the independent risk factors for PHC.The probability of PHC in Child-Pugh stage A is higher than that in Child-Pugh stage B and C.

Objective To evaluate the application effect of mind mapping in the optimization of reception process of critical patients in emergency service.Methods A total of 98 critical patients in the emergency ward of Zhongnan Hospital of Wuhan University were selected as the research object by convenient sampling method. They were divided into the control group (48 patients admitted from June 2014 to May 2015) and the observation group (50 patients admitted from June 2015 to May 2016). Patients in the control group were receipted by on-duty nurses complying with the standard made by emergency ward including the checklist of substance use,the reception flowchart of critical patients and admission information. 30 nurses in the emergency ward were trained with the mind mapping from May 2015. Patients in the observation group were receipted by trained nurses with mind mapping which was integrated to the reception standard including all the items mentioned above. The reception effects in two groups were compared and evaluated.Results Compared with the control group,the pass rates of fundamental nursing,the professional nursing and the disease treatment were significantly higher in the observation group (92.00% vs 64.58%,χ2=3.06; 94.00% vs 75.00 %,χ2=2.33;90.00 % vs 66.67%,χ2=2.57;allP<0.05). The incidence of complaints,the leakage rate of substance use time and the reception time were significantly lower in the observation group compared to the data in the control group [4.00 % vs 25%,χ2=2.68;18.00% vs 79.17%,t=5.86;(7.75±3.12) min vs (12.55±4.13) min,t=6.51;allP<0.05]. The satisfaction degree of doctors in clear division of labor,comprehensive treatment evaluation, execution of nursing measures toward to nurses were all significantly higher in the observation group compared to the control group (P<0.05).Conclusions The application of mind mapping optimizes the reception process of critical patients in emergency wards,guarantees medical safety of patients and promotes the nursing quality. It is worth using widely in clinical work.

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.