Objective To determine the susceptibility of Ureaplasma urealyticum(Uu) to 7 kinds of antimicrobial agents, to compare the result with tetracycline resistant determinant(tetM) detected by PCR technique.Methods Forty two Uu isolates were subcultured for 3 generations and then cultured with drugs with a series of concentrations from 64?g/ml ～ 0 06?g/ml. MIC was determined when the color of culture medium did not change from yellow to red in 72h culture. The tetM determinant in the isolates was detected by PCR with specific primers, and PCR products were digested by Taq 1 restrictive endonuclease. Results The antimicrobial activity of tetracyclines to Uu was low. No Uu strains resistant simultaneously to 3 groups of antibiotics were found in the study. Detection rate of tetM was 71 4% with correspondent digested fragments. Conclusion The susceptibility of Uu isolates to tetracycline group was low, but higher to macrolides and fluoroquinolones. Uu strains carrying tetM determinant might become drug resistant ones which should be monitored and followed up with MIC determination.

Objective To evaluate the application of 1H-MRS in chronic liver diseases related with hepatitis B, and to investigate the method of eliminating breathing influence to proton MR spectroscopy (1H-MRS) on liver. Methods Liver spectroscopy of 46 subjects were obtained by single-voxel PRESS sequence with respiratory gating with GE 1.5T scanner. The subjects were divided into 4 groups: the normal male group (10 cases), the normal female group (10 cases), the male hepatitis B cirrhosis group (14 cases) and the male liver cancer after hepatitis B group (12 cases) according to clinical data, MR manifestations and pathologic findings. Glutamine and glutamate complex (Glx) peak height served as a reference standard to judge the choline (Cho) peak height. The differences of Cho peak height of each group were compared. Results The peaks of Cho were lower than those of Glx in normal male group. The peaks of Cho were higher than those of Glx in 8 women of normal female group. The peaks of Cho were higher than those of Glx in 11 patients of the male hepatitis B cirrhosis group. The peaks of Cho were higher than those of Glx in 10 patients of the male liver cancer after hepatitis B group. The differences of Cho peak were statistically significant between normal male group and normal female group, the male hepatitis B cirrhosis group and the normal male group, the male liver cancer after hepatitis B group and the normal male group. Conclusion The peaks of Cho in normal males are lower than those of Glx. The peaks of Cho in the majority of normal females are higher than those of Glx. The peaks of Cho increase in male hepatitis B cirrhosis and liver cancer after hepatitis B. Respiratory gating can effectively eliminate the impact of breathing movement on proton MR spectroscopy of liver.

Non-surgical treatment programs play an essential position among hepatocellular carcinoma therapies. Precise evaluation of treatment efficacy has significant value in determination of non-surgical treatment programs for hepatocellular carcinoma. As the treatment methodologies and philosophy progress, the evaluation standards for the efficacy of non-surgical treatment of hepatocellular carcinoma are constantly updated and developed. This paper generalizes the development process of the evaluation criteria for the efficacy of non-surgical treatment of hepatocellular carcinoma, summarizes the evaluation criteria that have been and are currently applied in the clinical field in general, in order to provide a basis for patients to obtain timely and effective evaluation and treatment, and gives an outlook on the future development of the evaluation standards.