Radioactive esophagitis is one of the most common complications in thoracic tumor radiotherapy,biological factors such as single nucleotide polymorphisms (SNPs),miRNAs,and HIV infection may play key roles in the occurrence and development of radioactive esophagitis,and they have become an active field in protection areas of radiotherapy.We can identify the patients who may cause radioactive esophageal in high dose radiotherapy as early as possible,and modify the treatment plan to protect the esophagus.Therefore the biological factors of radioactive esophagitis are of important clinical significance.

Objective To explore the characteristics of community empty-nesters sleep quality and psychological defense mechanism,and the correlation between them.Methods 230 empty-nest elderly and 181 nonempty nesters were assessed with Pittsburgh sleep quality index (PSQI) and Defense style questionnaire (DSQ).Results PSQI score of empty-nest ehlerly was (14.33±2.48),higher than that of non-empty nesters(t=17.857,P＜0.01).Factor score of immature psychological defense style in DSQ was (3.92± 1.20),for the mediate defense style was (4.49± 1.11),for the wrapping factor was (3.38± 1.92).All of them were higher than the scores for nonempty nesters,and the differences were statistically significant (t=4.463,6.937,5.404,P＜0.01).Factor score for mature psychological defense style in DSQ was (3.96±1.45),and lower than the score for non-empty nesters(t=7.472,P＜0.01).hnmature defense mechanism of empty-nest elderly was positively related with subjective sleep quality,sleep continuity,sleep disorders,using sleep medicine,dysfunction in the daytime,and the total score in PSQ1 (r=0.533,0.145,0.605,0.521,0.497,0.734).Mature defense mechanism was negative related with sleep latency,sleep continuity,and the total score in PSQI (r=-0.129,-0.542,-0.104).The mediate defense meehanism was positively related with subjective sleep quality,sleep latency and the total score in PSQI (r=0.186,0.294,0.131),while negative related with sleep efficiency(r=-0.188).The wrapping factor had a positive correlation with subjective sleep quality,using sleep medicine and the total score in PSQI (r=0.440,0.431,0.564).Conclusion Empty-nesters tend to adopt immature defense styles,and most of them have a poor sleeping,which are closely related with each other.

Objective To investigate the protective effect of Tanreqing injection on the mice infected with influenza virus FM 1 . Methods The experiment includes protection of Tanreqing injection to the mice infected with influenza virus FM1, and effect of Tanreqing injection to the viral titers, pathology and lung index of mice with influenza virus FM 1 . Results Tanreqing injection can reduce the mortality of the mice with influenza virus FM 1 infected. And Tanreqing injection can improve viral titers, pathology, and lung index of the mice infected with influenza virus FM1. Conclusions Tanreqing injection can protect the mice infected with influenza virus FM 1 by resisting the influenza virus and meliorating the lung pathology of the mice infected with influenza virus FM 1 .

Objective To study the effect of elemene on transcription factor ELK1 and its target gene in human cervix cancer Hela cell. Methods The cell proliferation was assessed by MTT assay. The luciferase activity of transcription factor ELK1 was determined by the Dual-Luciferase Reporter Assay System. The protein expression of phosphorated ELK1 and its target gene c-fos were determined by Western Blot. Results Elemene can remarkably inhibited the growth of Hela cell and its IC50 was 80.6 ?g/mL. The luciferase activity of transcription factor ELK1 in Hela cells treated with elemene was inhibited. The protein expression of phosphorated ELK1 and its target gene c-fos in Hela cells treated with elemene were down-regulated. Conclusion Elemene can inhibit human cervix cancer Hela cell proliferation,which may be related with suppression of c-fos gene through inhibiting expression of phosphorated ELK1.

Hypoxia is an inherent feature of the majority of solid tumors,which can increase the resistance of tumor cells to radiotherapy and chemotherapy,promote tumor angiogenesis and lead to poor prognosis.Therefore,targeting the hypoxic tumor cells has become a spot in cancer therapy.Bioreduction drugs can be activated by a specific reduction to become cytotoxic metabolites,thus killing hypoxic tumor cells,while small molecular targeting inhibitors can selectively act on the key point of hypoxia pathway.They have paved a new way for hypoxia targeted therapy.

Objective To study the angiographic anatomic characteristics of renal arteries in Chinese.Methods Selective renal angiography was performed immediately after coronary artery angiography in(1?057) Chinese patients,including 702 males and 355 females.The mean age of them were 59.3?8.3 years.Six French JR4 catheters were used via transfemoral approach.Three to five milliliters nonionic contrast media were injected manually into each renal artery and cine pictures were taken at an anterior-posterior view.Results A single-renal-artery was found in most of the patients(87.23% at the left side and 89.97% at the right side).In the others,two or even more independent renal arteries were found at one or both sides.Most of the renal arteries (87.79% at the left side and 91.20% at the right side) originated between the upper third of the first lumbar vertebra and the upper third of the second lumbar vertebra.The right renal artery ostium was located superior to the left one in 45.13% of the cases.In the other 45.41% of them,renal artery ostia of both sides were at a same level.Conclusion Anatomic variations in the number and origin of renal arteries are not nare in Chinese.Understanding the angiographic anatomic characteristics of renal artery would be useful for renal artery interventions.

OBJECTIVE:To optimize the extraction technology of Baiyi rectal solution. METHODS:The effects of water amount and extraction time on extraction technology were investigated by central composite design-response surface method using OD value of berberine hydrochloride and ferulic acid and the extract yield as index. Validation test was also conducted. RESULTS:The optimal extraction technology was as 11-fold water,decocting 70 min for the first time;5-fold water,decocting 30 min for the second time. In validation test,OD value of first extraction was 0.940 0,and the bias between observed and predicted values was -2.07%;that of second extraction was 0.851 8,and the bias was -2.41%. CONCLUSIONS:The extraction technology of Baiyi rectal solution is reasonable,feasible and suitable for industrial production.

Objective To prepare the human bone sialoprotein (BSP) monoclonal antibodies (mAb)with high titer and specificity and identify its characterization,which is based on further studying BSP as clinical biomarker for breast cancer metastasizing to bone. Methods BALB/c mice were immunized with purified recombinant BSP protein.Cell fusion was performed between mouse splenic cells and myeloma cells (Sp2/0), and then the hybridoma cell lines secreting mAb against BSP antigen were screened and cloned. The ascites were prepared and purified with Protein G affinity chromatography.The titer and subtypes of mAb against BSP were identified and measured by ELISA and Western blotting analysis. ResultsNine hybridoma cell lines that stably secreted mAb against BSP were successfully obtained.Two of them,D001 and D002,were further identified, which belonged to the subtypes of IgG1 and κ light chain. The two antibodies titers in culture supernatant were 1∶5120 and 1∶10 240, respectively, and those in the ascites fluid were 1∶25 600 and 1∶51 200,respectively.Results of Western blotting analysis and immunohistochemistry showed that the two antibodies could specifically bind with BSP derived from human breast cancer cells.ConclusionNine mAb against BSP have been successfully prepared which can be used for further studying the biological properties of BSP and reveal its relationship with data from clinic patients.

Objective To explore the imaging characteristics of seminomas and nonseminomatous germ cell tumors (NSGCT) and its pathological foundation. Methods CT and MR imaging manifestations in 25 cases of testicular germ cell tumors proved by pathological examinations were analyzed retrospectively. All tumors were divided into seminomas group (12 cases) and NSGCT group (13 cases). In the seminomas group, 5 cases were examined by CT and 4 of those also had contrast enhanced CT. Seven cases had MRI and 4 of those had dynamic enhanced MRI. In the NSGCT group, 5 were examined by plain CT in which two were by contrast enhanced CT, eight were by MRI in which 4 were by dynamic enhanced MRI. CT or MRI characteristics (morphology, density or intensity, enhancement) in both groups were analyzed by Fisher test. Results Histological examination revealed 25 intratesticular lesions. In 12 seminomas, 10 showed a nodular/lobulated shape, 5 showed a mixed density or intensity. In 13 NSGCT, only one lesion showed a lobular shape, 11 showed a mixed density or intensity. Seven seminomas showed a low signal on T2WI on MRI while only two NSGCT showed this sign. In four lesions underwent dynamic MRI scanning, 3 showed fibrous septum enhancement while no lesions in NSGCT showed this sign. The occurrence rate of the above imaging characteristics in both group was significantly different (P<0.05). Conclusion Seminomas and NSGCT may have their own CT and MRI characteristics, which may be of great value for differential diagnosis .

Objective To investigate the clinical and pathological features of anorectal malignant melanoma ( AMM) .Methods The clinical pathological and follow-up data of 7 cases of anorectal malignant melanoma were collected.The clinical and histopathological features and immunophenotype were analyzed .Results Among the 7 cases, 5 cases were female, 2 cases were male, aged 50-68 years, the average was 61 years.The tumors located in the lower rectum in 1 cases, 2 cases of teeth near the line , 4 cases of anal anus .The structure and morphology of the cancer cells were diverse .The organizational structures were mainly diffuse , focal and acinar .The cellular morphologies were mainly epithelioid , spindle and lymphocyte like .And the results of IHC showed the expression of the following three markers, vimentin, S-100 and HMB45, were strongly positive in cancer cells.However, the expression of CK , EMA and LCA were negative .And 2 cases of CEA expression were positive , LI Ki-67 was 20%-50%.Conclusions Anorectal malignant melanoma is one kind of rare malignant tumor and its characteristic morphology , immunophenotype could be helpful for diagnosis and differential diagnosis .