Incidence of knee periprosthetic fractures after total knee arthropoasty is increased,and is a challenging clinical problem. Treatment options include conservative and operative methods. Only the doctor have sufficient and systematic understanding of periprosthetic fractures and consider both advantages and dis-advantages before deciding the appropriate treatment, the satisfying treatment outcome will be got. we re-viewde the type, treatment methods and outcomes of periprosthetie fractures of total knee arthroplasty repor-ted in recent years.

Graduate students'mental health is becoming an important program in the fields of medicine,education and psychology in our country.The author studies the recent articles and writes this article to explain the standard,content,measurement and the intervention of the graduate student's psychology health,so as to improve the graduate student's psychology health level.

Objective To investigate effect of alendronate on OPG and RANKL in periprosthetic osdeolysis induced by polyethylene particles.Methods Twelve rabbits which had been implanted a titanium plug in femur by intercondylar notch were divided into two groups randomly,polyethylene particles were injected into the left knee joint,one received alendronate,and the other placebo as control.After 12 weeks,all rabbits were sacrificed.Periprosthetic tissues were observed by ELISA.Results The concentration of OPG in the experimental group was not higher than that of the control (P ＞0.05 ).But the concentration of RANKL in the experimental group was lower(P ＜0.01 ).And specific value of OPG/RANKL was higher in the experimental group ( P ＜ 0.05 ).Conclusion The therapy of alendronate can change the concentration of RANKL and specific value of OPG/RANKL in periprosthetic osdeolysis induced by polyethylene particles,inhibit aseptic loosening of prosthesis in rabbits.

Objective To develop a prototype of low pressure plant-cultivating facility, for studying the growing technique of higher plants under low pressure in manned deep space exploration, so as to lay foundations for establishing controlled ecological life support system (CELSS) in low pressure environment. Methods According to detailed demonstration of technical plan, blueprint design and machining of components, facility installment, debugging, trial operations and verification experiments were carried out. Results Two sets of low pressure plant-cultivating facilities were manufactured, both of them could be used to cultivate higher plant under low or normal pressure. They worked stably and their technical parameters such as temperature, relative humidity, air pressure, O2 partial pressure, CO2 partial pressure and water content of matrix were all monitored and controlled effectively. Conclusion Their main technical indexes meet design and experimental requirements, low pressure plant-cultivating experiments can be carried out with the facilities effectively.

Objective To investigate the characteristic of growth and development of vegetable at hypobaric environment and offer the cultivating technique of controlled ecological life support system for higher plant.Methods The means of comparative measurement was adopted to study the growth and physiological characteristic of lettuce under low and normal pressure(40.0 kPa,101.0 kPa),which was carried out with the low pressure plant-cultivating facility.Results Compared with normal pressure,germination rate of lettuce seed was eliminated by 7.62% under total pressure 40.0 kPa and oxygen partial pressure 8.4 kPa conditions;harvest time of lettuce was delayed,so biomass harvested at the same time reduced by 13.67% and content of total saccharide declined;the average rates of photosynthesis and transpiration were lower than those at normal pressure in 30 days after planting(DAP),but which reversed after that;release of C2H4 and shoot height reduced by 38.79% and 11.68%,but leaf area,ratio of root to total biomass and root vigor increased by 15.43%,2.57% and 40.53%,respectively.Conclusion Seed germination,growth and development of lettuce are affected in some degree at total pressure 40.0 kPa and oxygen partial pressure 8.4 kPa,some measures should be adopted to regulate the growth of lettuce.

Objective To investigate growing characteristics of plants grown at elevated CO2 concentrations in controlled environment and provide base for determination of biological components in future space controlled ecological life support system (CELSS).Methods Lettuce (Lactuca sativa var.Youmaicai) was cultivated at higher atmospheric CO2 concentration gradient (0.05%～2.0%).Its growing status,physiological characteristics and the content of nutrients were evaluated.Results There were some active effects of elevated CO2 (0.1% to 1.0%) on the average rates of photosynthesis and transpiration of the collective lettuce leaves and the contents of chlorophyll and carotenoid,but excessive CO2 (1.5% and 2.0%) weakened these effects,and even had some negative impacts.Lettuce could grow normally at elevated CO2 (0.1% to 2.0%),average shoot height and leaf area of lettuce plants treated at 0.1%～2.0% CO2 all increased,but the numbers of leaves eliminated gradually.The contents of nitrogen,potassium and Vc in plants decreased,phosphorus content rose and microelement contents had no obvious change in various CO2 treatments.Conclusion The lettuce can grow nearly normally at higher atmospheric CO2 concentration without serious growing inhibition or death,which indicated that the Youmaicai held stronger ability of tolerating higher concentration of CO2,so it's suitable to be determined as a candidate biological component in CELSS.

Objective To investigate the characteristics of higher plant grown in red and/or blue light and test the capability of red and/or blue light emitting diodes(LEDs) used as the artificial lights for plant growth in controlled ecological life support system(CELSS).Methods Lettuce(Lactuca sativa var.Youmaicai) was cultivated in sole white LED,sole red LED and combined red and blue LEDs,respectively.Its growing status,physiological characteristics and the content of nutrients were evaluated,which were adopted to select the best combination of red and blue LED for plant growth.Results Red light promoted the radial growth of lettuce plants,and blue light was propitious to their transverse extension and synthesis of chlorophyll and carotenoid;lettuce plants couldn't grow normally in sole red light;with the gradual increase of blue light proportion(5%~20%),there were some active effects on the growth of lettuce plants,for instance,the improvement of photosynthesis,transpiration and fluorescence(Fo,Fv/Fm and qN),the increasing of contents of chlorophyll,carotenoid and nutrients and the enhancement of total biomass;the adverse effects of blue LED on the growing of lettuce plants appeared gradually with the addition of blue light(25%~50%).Its biomass of shoot decreased significantly,which induced the increase of the ratio of root/shoot.Conclusion The combined red and blue LEDs is propitious to lettuce(var.Youmaicai) growth and the best lighting condition is the combined 90% red and 10% blue light.

Objective To select suitable vegetable for use in Controlled Ecological Life Support System for future long-term space missions. Method Five crops of lettuce(four Lactuca sativa L. var.crispa L.and one Lactuca sativa L. var.longifolia Lam), two crops of spinach(Spinacia oleracea L.), one rape(B.napus L.)and one common sowthisthe (Sonchus Oleraceus L.)were grown in a Controlled Ecological Life Support Technique Experimental System. Hydroponic nutrient solution, light, temperature and relative humidity were regulated, CO_2 levels were controlled at 500, 1 000, 1500 and 2 000 ?mol/mol, and light intensity were controlled at 100, 300, 500 and 700 ?mol/(m2?s). Canopy photosynthetic rate, transpiration rate and leaf aera were measured on day after planted (DAP) 25 and 30. Plants were harvested at maturity, and analyzed for total fresh weigh and proximate composition (crude fiber,nitrite and ?-carotene). Result Three crops of lettuce “nenlvnaiyou”, “dasusheng”,“youmaicai” ,and one common sowthisthe were selected. The favorite CO_2 concentration and light intensity 2 000 ?mol/mol, and 700 ?mol/(m2?s) respectively.Conclusion The four crops selected have high productivity, photosynthetic rates and transpiration rates, which can be considered as the basis of the vegetables to be used for future long-term space missions.

Objective To investigate the clinical efficiency of perforator pedicled propeller flaps for soft-tissue coverage of the lower leg and foot defects.Methods From July 2007 to December 2011,twentyfive cases with soft-tissue defects of the lower leg and foot were cured by using the perforator propeller flaps.The origins of the perforator were 18 cases from peroneal artery,six cases from posterior tibial artery and 1 case from dorsal foot artery.The minimum of the flap scale was 4-9 cm,and the maximum was 10-33 cm.Results All cases were followed-up from 1 to 26 months.All the flap pedicles were smooth with a satisfied appearance.The donor sites were sutured directly in 9 cases and cured with skin-grafting in 16 cases.Three cases suffered from vein congestion,two cases were cured by incisions in the distal parts of the flaps,one flap had necrosis of the distal 1/3 part and was cured by skin-grafting.Conclusion The perforator pedicled propeller flap is a simple,safe and useful flap.It has more advantages than other pedicled flaps for softtissue coverage of the lower leg and foot defects.

Objective A foundation for one of the biological components,Spirulina,to be applied in Controlled Ecological Life Support System(CELSS)would be laid with exploring effects of ionization radiation on the growth of Spirulina.Methods By using the ?-rays of ~(60)Co,Spirulina were irradiated.The dose of the ionization radiation covered 0,0.5,1.0,1.5,2.0,2.5,3.0 kGy.After irradiated,these Spirulina were cultured under the same conditions.The growth state,shape change,photodensity change,photosynthetic efficiency(O2 produced),and trophic physiological indexes of Spirulina or its solution,were observed,measured and analyzed.Its anti-irradiation ability was investigated.Results After irradiared with ~(60)Co ray,the growth rate,photosynthetic O2 produced efficiency,length of fila and other trophic physiological indexes were all affected in certain degree,but as compared to the higher plants,Spirulina have stronger radiation proof and self-rehabilitation capacity.Even if under high radiation condition(3.0 kGy),there was no complete death of cells,and the dose resulted in 50% death of the Spirulina was 2.0 kGy.Conclusion Spirulina has stronger ionization radiation proof and self-rehabilitation capacity,it can be considered as one of the key biological components in CELSS for future long-term space missions.