Objective In order to know the influence of health education on eomlianee in pa-tients with diabetes. Methods Selected 278 patients with diabetes in our hosptial were aeccepted di-abetes related health eduction, and then compared the compliance before and after the education among the patients. Results The compliance rate after the health education in the patients was significant higher than before. Conclusions Systemtic disease related health education can enhance patients'serf-care consciousness, changes their adverse life style, and then improve their compliance and keep the blood sugar in an ideal level.

Objective: To explore the protective effect and its mechanism of the traditional Chinese medicine complex, ferulic acid compound, on cerebral ischemia reperfusion in rats. Methods :Experimental cerebral ischemia reperfusion in rats was made by occluding the two- vessel and bleeding from tails. To observe the effect of ferulic acid compound on levels of endothelin - 1 (ET - 1) 、 calcitonin gene- related peptide (CGRP) 、 malonaldehyde (MDA) 、 energy metabolism 、 the expression of heat shock protein 70 (HSP70) and histopathological analysis in brain tissue. Results:Ferulic acid compound can decrease the level of ET- 1 、 MDA and lactic acid in brain after cerebral ischemia reperfusion in rats,and also improve the contents of CGRP 、 ATP and Na+ - K+ - ATPase activity.The effect of ferulic acid compound was related to dosages, and its effect was better than the positive salvia miltiorrhiza. Eerulic acid compound can increase the HSP70 expression significantly and alleviate cerebral ischemic changes histopathologically. Conclusion :The effect of ferulic acid compound in many ways may be related to its protection against ischemia reperfusion brain injury.

Objective: To study the protective effect of Sodium ferulate on rat brain microvascular endothelial cells(BMEC) injured by hypoxia.Methods: BMEC from Wistar rat cerebral cortex was cultured and placed in an anoxia chamber with 95%N2 and 5% CO2 for 12 hours.BMEC activity or injury was assayed by observing cell ultramicrostructures and measuring viability by methyl thiazolyl tetrazolium(MTT) assay,trypan blue staining(TBST) and the lactate dehydrogenase(LDH),a marker of cell death.Results: The BMEC activity and viability was decreased after hypoxia as assessed by MTT,TBST and LDH,The ultrastructure of BMEC was damaged after hypoxia.SF could enhance BMEC activity and viability and ameliorate hypoxia injury.Conclusions: Sodium ferulate exerts protective action on BMEC injured by hypoxia.

Disorder of sexual development(DSD) refers to a group of diseases with various causes and phenotypes.It has attracted great worldwide as the incidence is rising and the life-long consequences accompanying.Environmental endocrine disruptors(EED) are thought to have an impact of the sex differentiation process and induce disorder of sexual development.This review summarizes the mechanism of DSD that associated with EED and focus on the relationship between DSD and EED.

Objective:To study the nursing care of multisite cardiac pacemaker implantation in treat cardiac arrhythmia and dilated cardiomyopathy.Methods:On the different basis of partient health problems,gave nursing care by apply nursing process.Carry out health edurcation in different period,so that improve the effect of health education and prevent the electrode break off.Results:(1)Au the pacemaker electrodes were at the good place,no broke off or moved away.The sense and the threshold were normal.(2)The patients' cardic function were improved and cardiac arrhythmia were disappear or improved.(3)The wound had no infection.(4)All the patients knew self-supervise and attention knowledge well.Conclusion:Applying nursing proless,keeping right position,keeping intensive observation and giving effective health education are the assurance for the mplantation.

Objective To design and analyze the comprehensive application of wireless network in hospital.Methods The wireless network technology and the 802.11 criterions and the main application fields of wireless network in hospitals were introduced,and analyzed the application directions of wireless network in hospitals and summarized the main effects of wireless network at hospitals.Results The application of wireless network in hospital can be raised the hospital management level,the medical quality and the working efficiency effectively.Conclusion The application of wireless network in hospital is so ripe that better functionality and efficiency of hospital information system could be gave.

Hyperglycemia is common in patients with aneurysmal subarachnoid hemorrhage (aSAH),and it has important association with dehyed cerebral ischemia (DCI) and may result in poor prognosis.This article summarizes the mechanism of the elevated blood glucose after aSAH,the reasons of causing DCI,and the corresponding treatment measures.

Objective To investigate the comparison of rubber gloves collection bag, linear closing type plastic collection bags and plastic specimen collection bag is used for application effect of laparoscopic ovarian cyst resection. Methods From 2013 June to 2014 December underwent laparoscopic ovarian cyst resection in 90 cases, according to the first date of surgery were divided into 3 groups, from the standard sample collection bag for rubber gloves (A group) 30 cases, linear closing type plastic collection bag (B group) 30 cases, collecting bag plastic specimens (C group) 30 cases. Respectively calculate autologous peritoneal bag mouth opening into time, time to complete transection of tissue into the specimen collection bag time, specimen collection bag left abdominal contents to the in vitro observation time, specimen collection bags in use in the course of rupture occurrence rate of specimen content leakage. Results The 3 groups of patients with autologous abdominal time were (7.18 ± 0.61),(6.72 ± 0.57), (5.98 ± 0.30) s, the difference was not statistically significant (t=41.80, P>0.01). The 3 groups of specimens collected pouch specimens respectively (40.84± 6.27), (49.86 ± 6.10), (32.98 ± 3.93) s, group C open pocket to maintain state of distraction to amputated tissue fully into the specimen collection bag in the shortest time, with a group and B group, the difference was statistically significant (F=69.87, P<0.01). The 3 groups of specimens collected from the abdominal cavity were (498.39±55.60), (315.70±47.32), (448.65±54.90) s, the difference was statistically significant (F=96.18, P < 0.01). The 3 groups of specimens collected specimen bag collection bag rupture were 26.7% (8/ 30),10.0% (3/ 30),3.33% (1/ 30), group A of the most common (χ2=0.926, P < 0.05), The 3 groups of specimens collected the bag contents leakage were 23.30%(7/30), 6.67%(2/30)、3.33%(1/30), group A of frequency was the highest, and in group B and C, the difference had statistical significance (χ2=0.635, P<0.05). Conclusions Plastic type specimen collection bag for soft bag with built-in wire, the bag opening is a certain tension, easy operation by operation control, convenient and reliable safety, shorten operation time, reduce the occurrence of complications, reduce surgical risks and costs, and improve the office efficiency and quality of the operation.

Objective To construct the recombinant adenovirus encoding human IL-24 gene for future gene therapy. Methods The human IL-24 gene fragment was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination in bacteria. Then the recombinant adenovirus was transfected into 293T cells using Lipofectine DOTAP. The target gene was detected by polymerase chain reaction (PCR). The titer and its infection rate were determined using the green fluorescent protein (GFP) expression in the shuttle plasmid. The expression of target protein was measured by the method of immunohistochemistry. Results Restriction endonuclease and PCR analysis confirmed that the human IL-24 gene was successfully inserted into the adenovirus vector. The titer of the recombinant adenovirus was 1.2?10 10 pfu/ml. The adenovirus has a strong effect on A549 cells and human IL-24 can express in it. Conclusion The recombinant adenovirus containing human IL-24 gene was successfully constructed by the method of homogenous recombination in bacteria.

Objective To construct the recombinant adenovirus encoding human p16 gene for future gene therapy.Methods The human p16 gene fragment was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination in bacteria.Then the recombinant adenovirus was transfected into 293T cells using Lipofectine DOTAP.The target gene was detected by polymerase chain reaction(PCR).The titer and its infection rate were determined using the green fluorescent protein(GFP) expression in the shuttle plasmid.Results Restriction endonuclease and PCR analysis confirmed that the human p16 gene was successfully inserted into the adenovirus vector.The titer of the recombinant adenovirus was 6.1?10~(10)pfu/ml.The adenovirus has a strong effect on human fibroblast cells.Conclusion The recombinant adenovirus containing human p16 gene was successfully constructed by the method of homogenous recombination in bacteria.