Objective To explore relationship among different degrees of hypertension and plasma homocysteine and their clinical significance. Methods According to ,95 hypertension patients were collected and divided into mild,moderate and severe groups,65 patients from the healthy check-up selected as control group, then the plasma Hcy level was measured and compared between the groups. Results Compared with the normal group ( 12. 5 ± 3. 1 ) μ mol/L, Hcy plasma levels of mild ( 15.4 ± 4. 8 ) μmol/L ( t = - 4. 543, P ＜ 0. 01 ), moderate ( 23.2 ± 5. 6 ) μmol/L ( t = - 14. 291, P ＜ 0. 01 ), severe (28. 1 ± 6. 7) μmol/L( t = - 18. 090,P ＜0. 01 ) hypertension group increased significantly,severe group was obviously higher than patients among moderate group,moderate group was significantly higher than patients among mild group,statistical significance among different groups were found( F = 118. 550, P ＜ 0. 01 ). Conclusion High Hcy was an important independent risk factor,the monitoring of plasma homocysteine level for hypertension contribute to diagnosis and understand disease progression.

Objective To investigate the expression of Notch pathway in the kidney of diabetic nephropathy (DN) rats and the intervention effect of Chinese materia medica (CMM) for dispersing blood stasis and dredging collateral. Methods Sixty male Sprague-Dawley rats, in which ten rats were randomly selected as control group (n = 10), and the other rats were fed with high glucose and high fat diet combined with low-dose streptozotocin (STZ) ip injection as DN models. The model rats were randomly divided into model group, irbesartan group, and Huayu Tongluo Granles (HTG) group. The rats in each group were ig administered with corresponding drugs. At the end of the 16 weeks, the 24 h urinary protein was detected. The expression of Notch1, Jagged1, and Hey1 mRNA and protein in renal tissue was detected by real-time PCR, and immunohistochemical staining and western blotting assay, respectively. Results Compared with the control group, 24 h urinary protein, the mRNA and protein expression of Notch1, Jagged1, and Hey1 in model group was significantly increased (P < 0.01). Compared with the model group, 24 h urinary protein and Notch1, Jagged1, Hey1 mRNA, and protein expression of HTG group and irbesartan group was significantly decreased (P < 0.01). Conclusion CMM for dispersing blood stasis and dredging collateral can reduce 24 h urinary protein in DN rats and inhibit the high expression of Notch1, Jagged1, and Hey1 in the kidney tissue of DN rats, which may be one of the main ways to reduce proteinuria excretion.

Thromboembolic diseases are major health problems worldwide,and remain the leading cause of mortality and disabil-ity at present.Bleeding is the most important complication of an-tithrombotic therapy for thromboembolism,therefore research and development of new antithrombotic drugs with lowered bleeding risk is a significant medical need.The data that elevated plasma levels of FXI are associated with thromboembolic diseases,se-vere FXI deficiency reduced incidence of DVT and ischemic stroke,and FXI deficiency or inhibition in animals shows protec-tive effects against thrombus formation supporting FXI as a novel antithrombotic target with lowered bleeding risk.This paper re-views the progress on FXI as a novel antithrombotic target and the inhibitors target FXI.

Objective:To investigate the specific immune act ivated effects in the mouse immunized by MHC I-restriced tumor antigen peptide pulsed dendritic cells(DC) with IL-2 gene modified.Methods:DC were transfected with IL-2 gene via adenovirus vector(DC-IL-2) the mRNA of mIL-2 was examined by RT-PCR.Syngeneic mice were immunized by DC-IL-2 pul sed with MHC-I-restricted Mutl tumor antigen peptiede of 3LL Lewis lung carcin oma (DC-IL-2-Mutl),the effects of lymphocyte subsets in the draining lymphoid node from the tumor antigen pulsed DCs with gene modified immunized mice by flo w-cytometric analysis(FACS) analysis.Results:The mRNA of mIL-2 was expressed in the DCs.The ratio of CD8+T cells was hosted in the dra ining lymphoid node from Mutl pulsed DCs(DC-Mutl) immunized mice.The ratio of C D8+T cells and NK cells were all hosted obviously in the draining lymphoid nod e from DC-IL-2-Mutl immunized mice .Conclusion:Immunized mice with DC-IL-2-Mutl can induce the mouse specific antitumor immunized affect in vivo and can activate a variety of immunized effect in the mouse.

Objective To explore the changes of expression levels of 12S rRNA and cytochrome oxidase subunit Ⅰ (COXⅠ) mRNA encoded by mtDNA in rat cerebral cortex after rat exposure to hypobaric hypoxia for different days. Methods Healthy male Wistar rats were exposed to hypobaric chamber simulating 5 000 m above sea level (23 5 h/day) for 2, 5, 15 and 30 d. Rats in the control group were not exposed to hypoxia. Rats were sacrificed by decapitation. Total RNA in cerebral cortex was extracted using a standard program. Transcriptional levels of 12S rRNA and COXⅠ mRNA were determined by reverse transcription polymerase chain reaction (RT PCR). Results Compared with that in the control, the expression of 12S rRNA increased by 57% after hypoxic exposure for 2 d ( P 0 05). Compared with that in the control group, the expression of COXⅠ mRNA increased significantly by 55% and 106% after hypoxic exposure for 2 and 5 d ( P 0 05). Conclusion Hypoxic exposure may have effect on both protein gene and ribosome gene expression encoded by mtDNA, and the expression changes in a hypoxic exposure time dependent manner. This suggests that hypoxia can have effect on mitochondrial oxidative phosphorylation gene expression at both mitochondrial transcriptional and translational levels.

Objective To explore basic regularities in sinew lesion distribution in patients with knee osteoarthritis. Method Two hundred and thirty knee osteoarthritis patients with 253 affected knees were selected. Under the sinew theory, the positions and frequency of sinew foci around the knee joint were counted by palpation. Result In the distribution of foci, the total frequency accounted for 39.1% in the Foot-Yangming. Of them, the single Foot-Yangming sinew type accounted for 23.3% and the complex type accounted for 48.5%. The total frequency accounted for 32.4% in the Foot-Taiyang. Of them, the single Foot-Taiyang sinew type accounted for 21.3%, the complex type accounted for 44.5%. The Foot-Yangming + Foot-Taiyang sinew lesions accounted for 14.6% and the single foot-three-yin sinew type accounted for 15.0%. Conclusion Knee osteoarthritis of Foot-Yangming or Foot-Taiyang sinew type should be treated from “sinew”.

The canonical Wnt signaling pathway has been the hot spot in the signaling pathway studies which are related to ovary carcinogenesis in recent years,whose activation may induce the multiplication and self-renewal,survival and metastasis of ovarian cancer stem cells.The target gene mutations of key members in the canonical Wnt pathway and abnormal expresses of negative regulation molecules are closely associated with the occurrence and development of ovarian cancer.Deeply investigating the regulative mechanisms of canonical Wnt signaling pathway in ovarian cancer,and taking this as the therapeutic target to conduct the basic research and preclinical experiment may provide theoretical basis for treating better ovarian cancer patients in clinical practice.

AIM: To study the changes in capillarity of skeletal muscle during acclimation to high altitude, and explore the effects of a certain extent physical activity under hypoxia on capillary formation and the role of vascular endothelial growth factor (VEGF) in this process. METHODS: 48 Wistar rats were divided into 3 groups: Ⅰ normoxic control; Ⅱ hypoxia and Ⅲ hypoxia+exercise. Rats of Ⅱ and Ⅲ groups were subjected to hypobaric hypoxia for 5 weeks (23 h/d). They were first brought to simulated 4 000 m altitude, where rats of the Ⅲgroup were forced to swim for 1 h/d (6 d/week). Then the animals were ascent to 5 000 m. Biomicrosphere method was used to determine blood flow of skeletal muscle. The mean fiber cross-sectional area (FCSA), capillary density (CD) and capillary/fiber ratio (C/F) of red portion of the lateral head of the gastrocneminus were assayed by myofibrillar ATPase histochemistry. VEGF and its receptor KDR were assayed with immunohistochemistry method.RESULTS: By comparison with the normoxic control, 5-week hypoxic exposure resulted in a decrease in cross-sectional area of skeletal muscle fiber and an increase in CD, but the C/F remained unchanged. The blood supply to the gastrocnemius was not changed. After 5-week-exercise at high altitude, the muscle fibers did not undergo atrophy. CD, C/F, and the blood flow at rest increased significantly. VEGF protein was found primarily in the matrix between muscle fibers; KDR were shown mainly in endothelial cells of capillary. VEGF was more strongly stained in the skeletal muscle of hypoxia-exercise rats.CONCLUSION: Hypoxia itself can not induce neovascularization. While exercise during hypoxic exposure can lead to capillary formation. VEGF and KDR may play roles in it. New capillary formation benefits the blood supply, oxygen delivery and working performance at high altitude.

Objective To investigate the safety and feasibility of robot-assisted laparoscopic right hemicolectomy for colonic cancer. Methods These 5 patients with ascending colonic cancer received robot-assisted laparoscopic right hemicolectomy. Results All operations were performed successfully. There was no postoperative complications. Da Vinci surgical system was found to be associated with fewer hemorrhage, rapid postoperative intestinal recovery, and therefore a shorter hospital stay. Conclusions Robot-assisted laparoscopic right hemicolectomy can be applied safely and with feasibility for colonic cancer.

Objective To evaluate the clinical application of a novel hepatitis B virus YMDD mutation DNA diagnostic kit (magnetic beads method kit).Methods A total of 324 HBV clinical serum samples was tested with the magnetic beads method kit and another kind of fluorescence diagnostic kit (boiling method).Accuracy, specificity, and sensitivity were compared.Results The consistency of positive detection rate of two kits was 100％ (95％ CI : 98.0％ ～ 100％), negative consistency was 97.12％ (95％ CI : 92.8％ ～99.2％) and the total consistency was 98.76％ (95％ CI : 96.9％ ～99.7％).Four cases of discrepant samples were confirmed by sequencing, and statistical analysis performed by Kappa test (Kappa =0.975) shows good consistency between the two methods.Conclusions The magnetic beads method kit has good consistency compared to the regular boiling method kit, and the polymerase chain reaction (PCR) detection system contains an internal positive control (internal control) to avoid a false negative resuit, which is more suitable for clinical diagnosis.