Based on the analysis of the standards of bacteriological quality monitoring of laboratory animals in developed countries, this paper provides suggestion for modification of bacteriological quality monitoring of laboratory mice and rats in China.

Objective To explore the implementation of the reservation case teaching method in the teaching of medical ethics.Methods:96 students of 2 classes selected from 2010 grade clinical medicine specialty by random cluster sampling ( trial group) were taught with the reservation case teaching method , while 85 students of another 2 classes selected from the same grade and specialty ( control group ) were adopted with the traditional teaching meth-od .Then the effect of the reservation case teaching method was evaluated by test and questionnaire .Results:Most of the students in the trial group considered the reservation case teaching method are beneficial to strengthen the learning interest , understand and know more about the medical ethics , cultivate creative thinking , enhance the a-bility of self-study and solving practical problems .Conclusion:Reservation case teaching method can be used in medical ethics teaching , which is advantageous over the traditional teaching method .

p21 activated kinase 4 (PAK4) is a member of a family of serine/threonine kinase.PAK4plays an important role in a variety of cellular functions including cell cycle,cell proliferation,apoptosis,and cytoskeletal reorganization.Recently,PAK4 has been shown to overexpress in a variety of malignancies,and contribute to cancer cell migration and invasion through multiple signalling pathways.

Objective To discuss the non-antibiotic Chinese medicine therapy for the non-gonococcus conjunctivitis in newborns, and reduce the use of antibiotic and the production of bear-medicine-bacterium. Methods 0.9%-sodium chloride was used as the inoculation fluid, Shuanghuanglian (freezing and drying) was uscd as dissolution injection. Dexamethasone sodium phosphate injection 1ml: 5mg was added, mixed-fluid 16ml was matched to spray the eyes after ultrasonic-atomization equipment atomizes. Results 56 examples 103 eyes which had the nongonococcus conjunctivitis in newborns were treated only with the injection with the Shuanghuanglian dexamethasone sodium phosphate injection mixed with the fluided ultrasonic-atomization equipment atomizes and the total effectiveness reached 97.08%. Conclusion The Shuanghuanglian-ultrasonic-atomization way to treat non-gonococcal conjunctivitis in the newborns is safe and effective.

Objective To assess antibiotic prescription habits,cost pattern and the prospective intervention in Intensive Care Unit was analyzed.Methods Data on antibiotic utilization and antibiotics susceptibility were col-lected prospectively from individual electronic charts from July 2014 to September 2014.Results 225 of 246 patients surveyed used antimicrobial during the ICU stay,and antibacterial drug utilization rate was 91.46%.Cefperazone-sulbactam and piperacillin-tazobactam were the most prescribed medications.Total defined daily dose ( DDDs) was 1121.1 DDDs.Bacteria culture was 98 positive in 677 cases and 101 pathogenic bacteria were isolated.Conclusion Interventional programs should focus on promoting infectious control with rational antibiotic prescription aimed at mini-mizing the future emergence of bacterial resistance and futile.

Objective To discuss the role of naso-carbon in the protection of parathyroid during surgery. Methods 72 patients were randomly divided into the two groups,Injecting 0.1-0.2mL nano-carbon in the thyroid during a thyroid surgery.When the thyroid and surrounding lymph tissue were stained,then the thyroid surgery should be continued,the non-stained tissue should be protected.Results There were 6 cases with hypocalcemia in the con-trol group and 2 cases in the observation group(χ2 =13.87,P<0.05).5 cases with PTH decrease in the control group and 1 case in the observation group(χ2 =14.53,P<0.05).7 parathyroid glands were examined in the control group,while none in the observation group(χ2 =127.54,P<0.05).Conclusion Using naso-carbon during the thy-roid surgery,parathyroid gland cannot be stained black.The parathyroid gland can be easily identified and preserved.

Objective To know the drinking water quality in villages of Tongzhou District,Beijing and provide the basic data for government. Methods 623 wells in 455 villages were sampled in 2004 and the water samples were tested with the national standard methods. Results 166 of 623 water samples were up to standard,the qualification rate was 26.65%. The drinking water quality of all villages was different,in Maqiao(58.62%) and Liyuan(76.92%) it was better than that in Xiji,Huoxian,Zhangwan,Songzhuang. The qualification rate in Xiji was only 10.26%. In the present investigation,24 water quality parameters were tested and 14 parameters exceeded the limit,such as NH3-N,iron,total bacterial count,coliform group,fluoride and arsenic. Some sanitary problems were also found. Conclusion The ground water in Tongzhou has been contaminated,especially the shallow layer ground water,more attention must be paid to improvement of drinking water quality in these villages.

Objective To explore CT features and clues to diagnosis of rhabdmyosarcoma(RMS) in head and neck .Methods 14 cases of rhabdmyosarcoma scanned by CT were co llected retrospectively during 1992～2001 and comparatively studied by the age o f patients and the pathologic types. Results Children and adolecence were mainly involved in R MS in head and neck which pathologic types were embryonal type and adeno-vesicle type ,and the former was the main type .CT features of rhabdomyosarcoma include d round and oval masses which had a homogeneous high density and close relation to muscles ,obvious enhancement after injection of contrast media ,often complic ated with the wide destruction of bone and other adjacent tissues,the collecting lymph node enlargment.Conclusion CT findings of rhabdomyosarcoma in head and ne ck had some features and combined with the age of patients can be made the diagn osis of RMS.

Objective [WT5”BZ] To study the relationship between human papillomavirus (HPV) 16 and its serum antibody in the development of gastric carcinoma (GC) [WT5”HZ] Methods [WT5”BZ] Polymerase chain reaction (PCR) technique was used to detect HPV DNA in 42 fresh GC specimens and 42 fresh normal gastric mucosa adjacent to the tumour (NGMAT). Enzyme linked immunosorbent assay (ELISA) was used to screen the serum HPV16 antibody(Ab) of 42 GC patients and 46 controls using HPV16 virus like particles (VLPs) which was produced by recombinant bacilovirus in insect cells [WT5”HZ] Results [WT5”BZ] HPV16 DNA was found in 26 2% (11/42) of GC specimens,but in none (0/42) of NGMAT ( P

Objective To observe the change of renal function and cell apoptosis after injecting SB203580 before and after reperfusion, and investigate the protective role of p38 MAPK inhibitor SB203580 for ischemic/reperfused kidney in rats. Methods p38 MAPK inhibitor SB203580 was injected by tail vein into rats with ischemic kidney before and after reperfusion. The plasma levels of creatine and BUN were measured at various time points. The apoptotic rate in the renal tissue at various time points was determined using TUNEL. Results Administering SB203580 before reperfusion could decrease renal cell apoptotic rate, and renal function damage. Administering SB203580 after reperfusion had not obvious effect on the renal function and cell apoptosis. Conclusion Administering p38 MAPK inhibitor before reperfusion can attenuate post-ischemic renal fuction damage and cell apoptosis.