Background Reliable intraocular pressure (IOP) measurement is essential in glaucomatous study.TonoLab is a newly designed tonometer for small animals,however,its repeatability and reproducibility remains unknown.Objective This study aimed to evaluate the repeatability and reproducibility of TonoLab rebound tonometer in DBA/2J mice.Methods Intraocular pressure was measured in both eyes of 63 DBA/2J mice aged 7 months under systemic anesthesia with TonoLab rebound tonometer.IOP of each eye was measured for 3 times by two observers independently and consecutively using TonoLab rebound tonometer,and the data were obtained in single blind method.Within standard deviation (Sw),interclass correlation coefficient (ICC),within coefficient of variation (CVw) and corresponding 95％ confidence interval (CI) were calculated to evaluate the repeatability of measuring result from single observer,and Bland-Altman method was used to assess the agreement between two observers.The abnormal data which affected repeatability was analyzed.Results Among the 126 eyes,105 eyes showed the normal IOP with mean value (12.98 ± 1.05) mmHg from observer 1 and (12.41 ±0.92) mmHg from observer 2.Ocular hypertension was found in 21 eyes with mean value (27.76 ± 1.95) mmHg and (27.49 ± 1.28) mmHg for observer 1 and observer 2 respectively.Sw,ICC and CVw for observer 1/observer 2 were 1.34/1.11 mmHg,0.90/0.92,8.24％/7.42％ in normal eyes and 2.21/1.49 mmHg,0.89/0.95,6.97％/4.82％ in hypertensive eyes.The average bias between two measuring values from different observers was 0.60 mmHg in the normal eyes (95％ CI:-5.20-6.30 mmHg) and 0.30 mmHg in the hypertensive eyes (95％ CI:-7.30-7.80 mmHg).Conclusions TonoLab rebound tonometer can offer a good intraobserver repeatability in both normal and hypertensive eyes in mice,but the agreement between two observers is outside an acceptable level.So we consider IOP in mice obtained by TonoLab rebound tonometer is reliable and accurate from a single observer.

Objective:To study the effect of totally endoscopic surgery and Miccoli surgery for papillary thyroid microcarcinoma. Methods Totally 64 patients with papillary thyroid microcarcinoma from April 2013 to April 2014 in our hospital were divided into the ob-servation group and the control group according to a random number table.And the intraoperative and postoperative situation of the two groups were compared.Results The number of lymph node dissection,postoperative hospital stay,hospital costs of the two groups had no sig-nificant difference (P >0.05).The blood loss,operative time,drainage time,amount of drainage,incision length,time of postoperative recov-ery of the observation group were significantly less than those of the control group with statistical significance (P <0.05).But the cosmetic score of observation group after operation was lower than that of the control group,and there was a significant difference (P <0.05).Postop-erative pain grade of the observation group was better than the control group with statistical significance (P <0.05).The complication rate of control group was significantly higher than the observation group (P <0.05).Conclusion Miccoli surgery for papillary thyroid microcarci-noma has more advantages compared with totally endoscopic surgery in terms of blood loss,operative time,drainage time,amount of drainage, incision length,time of postoperative recovery,and degree of pain.Moreover,it has a lower complication rate and high security,which should be the first choice in clinic surgery.

Objective This study investigated the differences between axillary temperature monitored for 5min and 10min with mercury in glass thermometers. Methods A sample of 365 patients had axillary temperatures monitored for five minutes and ten minutes, Relevant research data were analyzed by meta analysis. Results At α=0.05 level, the result of paired t test between 5 minutes and 10 minutes temperature indicated that there was statistical difference, Meta analysis had the same result, Temperaturedata of 365 patients were expressed in the Bland-Altman plot which displayed that their consistency can not be accepted in the professional. Conclusions The optimal axillary mercury thermometer placement time for recording human body temperature is 10 minutes.

BACKGROUND:Three-dimensional (3D) motion analysis system combined with electromyograph cannot only objectively analyze movement function of the upper limb, but also exert an important role in exploring the exercise-related factors and the underlying mechanism.
OBJECTIVE:To explore the influence of movement velocity and target location on kinematics and muscle activity in upper limb reaching.
METHODS:Twelve healthy young adults reached for three different location targets (frontal, ipsilateral and contralateral) at self-selected and fast speeds to with the dominant right hand, respectively. Kinematic parameters and muscle activity were recorded by Vicon 3D motion analysis system and Noraxon wireless electromyograph, synchronously.
RESULTS AND CONCLUSION:Reach path ratio became smal er when moving faster (P<0.001), while mean velocity, peak velocity and the time percentage of peak velocity increased (P<0.001). Posterior deltoid, biceps brachi and triceps brachi activation was higher during fast speed movement (P<0.001). Muscle activation of anterior deltoid was lower in ipsitralateral reaching than contralateral (P=0.001) and frontal reaching (P<0.001), and posterior deltoid was higher than contralateral reaching (P=0.019). Biceps brachi (P=0.039) and triceps brachi (P<0.001) activation was also higher in ipsitralateral reaching than frontal reaching. These results suggest that moving fast can contribute to more muscle activation, high velocity, smooth trajectory and increased movement quality. Posterior deltoid, elbow extension and flexion muscle activation are high in ipsitralateral reaching, while high anterior deltoid activation in contralateral reaching;therefore, target location result in muscle synergies.

Objective To develop a humidification fluid dropping joint for the breathing machine to solve the problems in humidification fluid retension,pipeline leakage,pipeline fixation and etc.Methods A Infusion extension tube was involved in with 10 cm length left at the injector end.A hole was made at the side wall of the L-shaped joint of the breathing machine,whose internal diameter equaled to the external diameter of the extension tube.The extension tube was put into the joint through the hole,and the depth of imbedded tube was within 4 and 6 cm.Sealing and fixation at the connection between the tube and hole were executed with 502 glue and short tourniquet.Results The humidification fluid dropping joint could be connected with infusion apparatus of the pump or the infusion extension tube of the micro pump,which behaved well in eliminating accumulated humidification fluid,sputum suction,humidification and facilitating mechanical ventilation.Conclusion The joint developed gains advantages in easy manufacture,reducing complications and increasing the dependence on artificial airway,and thus is worthy promoting clinically.

Objective To investigate the compliance of evidence-based nursing on post-operative elders with hip fracture sitting at early stage. Methods A total of 108 post-operative elders with hip fracture were divided into the observation group and the control group with 54 cases in each group with random digit table. The patients in the control group sit from bed based on their state of vital signs and mental status on the 2nd or 3rd postoperative day. And those in the observation group employ evidence-based nursing on the basis of the control group. The degree of anxiety, postoperative fatigue and compliance of patients were compared. Results Two weeks after the operation, the degree of anxiety scored 40.96±5.13, postoperative fatigue scored 1.89±0.65 in the observation group, which were lower than those of the control group, 56.40±4.28, 4.55±0.71, the difference was significant (t=288.41, 412.35, both P<0.01). The rate of compliance in the observation group was 94.44% (51/54), and the rate of the control group was 77.77%(42/54). The rate of compliance in the observation group was higher than that of the control group(χ2=8.57, P<0.05). Conclusions Using evidence-based nursing on post-operative elders with hip fracture sitting at early stage is able to improve the compliance of patients. It is good for reducing the postoperative complications, improving activities of daily life and mental state, promoting rehabilitation in patients as well.

Objective To discuss the value of joint detection in patients with progressive cerebral infarction (PCI) on high sensi‐tivity c‐reactive protein(hs‐CRP) ,and homocysteine(Hcy) ,D‐Dimer ,Fibrinogen(Fib) ,ferritin(SF) .Methods According to the de‐velopment and evolution of the 156 patients with acute cerebral infarction were divided into progressive cerebral infarction group and non‐progressive cerebral infarction group ,to determine the level of serum hs‐CRP ,Hcy ,one or two D‐Dimer ,Fib ,SF .Results hs‐CRP ,Hcy ,D‐D ,Fib ,SF in the PCI group were higher than those in the control group ,the difference was statistically significant (P<0 .05) .Conclusion Between the development of the PCI with hs‐CRP ,Hcy ,D‐D ,Fib and SF ,there is a close relationship ,to detect the level has a certain significance to the prediction of progressive ischemic stroke .

Objective To evaluate the clinical efficacy and safety of a fractional bipolar radiofrequency device for the treatment of wrinkles of the face and neck.Methods This study enrolled 39 volunteers (including 37 females and 2 males aged 35-60 years) with Fitzpatrick skin type Ⅳ-Ⅴ and Fitzpatrick wrinkle scale score of 4-6.All the subjects received three sessions of treatment with a fractional bipolar radiofrequency device at intervals of 4-6 weeks.Follow-up visits were scheduled at one month after each treatment session for evaluation of efficacy (using standardized photography),subjective satisfaction and adverse effects.Results After three sessions of treatment,all the subjects experienced a significant decrease in Fitzpatrick wrinkle scale compared with baseline values,and wrinkle improvement score increased with the increase in treatment sessions.The Fitzpatrick wrinkle scale score for forehead wrinkles,glabella wrinkles,fishtail lines and neck wrinkles in these subjects after three sessions of treatment significandy differed from that before treatment (all P ＜ 0.01).Significant differences were also observed in the wrinkle improvement score for forehead wrinkles between these subjects after two sessions and one session of treatment (P ＜ 0.01),and observed in that for glabella wrinkles,fishtail lines and neck wrinkles between these subjects after three sessions and one session of treatment (P ＜ 0.05 or 0.01).Side effects were mild with no significant downtime.Conclusion Fractional bipolar radiofrequency may be a safe and effective option for the treatment of wrinkles of the face and neck in individuals with Fitzpatrick skin type Ⅳ-Ⅴ.

Objective To analyze the perceived control of lung cancer patients undergoing chemotherapy and to explore its influencing factors. Methods A total of 185 patients with lung cancer were recruited and assessed by homemade general information questionnaire and the Cancer Experience and Efficacy Scale. Results The total score of cancer experience during chemotherapy in patients with lung cancer was (62.50±6.99) points. The total score of efficacy during chemotherapy in patients with lung cancer was (42.52±8.08) points. Single factor analysis showed the influencing factors of perceived control were personal income, sex, education, metastasis, surgical treatment and stage of disease. Conclusions The cancer-related experience and efficacy of patients with lung cancer is at middle level. Health-care worker should pay attention to the impact of lung cancer patients and take effective measures to mitigate the negative cancer-related experience, thereby improving the perceived control of the lung cancer patients.

Objective:To review the research progress in non-injection administration systems of docetaxel. Methods: Based on the related literatures in recent five years, the studies on the application of docetaxel by non-injection ways including oral, transdermal, implantation and rectal administration were summarized and analyzed. Results:The docetaxel non-injection administration systems ex-hibit the advantages of convenient use, high bioavallability and low incidence of adverse drug reaction. . Conclusion:The non-injection administration systems of docetaxel show good development prospect.