Surgical treatment of colon cancer is the first choice in the therapy stages,supplemented with systemicor local radiotherapy and chemotherapy.With the developement of molecular biology,there has been a variety of treatment program,some of these methods have been accepted in clinical testing stage.But the safety of the treatment on colon cancer is still a hot topic.

Acupuncture Therapy ; Humans ; Male ; Middle Aged ; Postoperative Complications ; therapy

Molecular targeted therapy is a treatment bed emerging recent years for the treatment of malignant tumors,imatinib mesylate in the treatment of gastrointestinal stromal tumor (GIST) is a successful example ofmolecular targeted therapy.Imatinib mesylate can inhibit tyrosine kinase andinhibition of cell proliferation by treatment of gastrointestinal stromal tumor.With the positive effect of imatinib in the treatment of GIST,the related adverse reactions and the prevention and treatment strategies are worth to be discussed.

The treatment of total avulsion of the hand is somewhat difficult and the result usually not satisfactory. Four cases of such patients were treated from 1983 to 1987. After routine debridement, digital nerves and its surrounding adipose tissue were preserved. An "S" shape skin flap was designed an raised in the contralateral upper abdominal quadrant to cover the injured hand like a bag, so that both sides of the hand were covered. The donor area (12cm2) was directly sutured. Nerve endings might grow into the flaps because of the preservation of digital nerves. Three patients were followed-up for 2 to 6 years, the injured hands regained good pain sensation as well as stereognostic and temperatuer sensations. The function of the hand is satisfactory, but the flap looked bulky and multiple plastic operations were necessary to seperate the fingers.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Digestive System Neoplasms ; complications ; surgery ; Female ; Humans ; Intestinal Obstruction ; etiology ; therapy ; Male ; Middle Aged ; Postoperative Complications ; therapy ; Young Adult

Objective To study the burden of main earegivers of patients with stroke and its influ-encing factors, besides, the corresponding nursing countermeasures were disucssed. Methods 56 main caregivers of stroke families were selected to complete caregiver burden inventory (CBI). Results The general level of burden of main caregivers was in the moderate level with score value (1.55±0.35),factors influencing the burden of caregivers included ADL index, number of caregivers who involved in nursing and hospitalization times. Conclusions Nurses should educate the stroke patients about their rehabilitation systematically to decrease the severity of stroke sequela and readmission times, besides, they should strengthen their family support to reduce the burden of caregivers and increase their health status both physically and psychologically.

Objective To investigate the effect of hypoxia-inducible factor (HIF)-1α and vascular en-dothelial growth factor (VEGF) on the angioge-nesis of rheumatoid arthritis (RA). Methods The collagen-induced arthritis (CIA) model was set up in male Wistar rats. The pathological angiogenesis and expression of HIF-1α and VEGF in the synovia different time points were observed by H&E and immunohistochemistry staining. Results The expressions of HIF-1α and VEGF on CIA synovium were significantly elevated and their expression increased gradually with the prolonged disease course. Both synovial HIF-1α expression and VEGF expression were correlated significantly with the pathological angiogenesis score. Synovial lining and sublining HIF-1α expression were correlated significantly with VEGF expression respectively. Conclusion H1F-1α may play an important role in the pathogenesis of RA by upregulating the expression of VEGF and then promoting angiogenesis.

Objective To investigate the effect of small intestine exclusion surgery on blood glucose and oral glucose tolerance test (OGTT) in Goto-Kakizaki (GK) rat with type Ⅱ diabetes mellitus.Methods 40 GK rats were randomly divided into 5 groups:duodenal bypass (Group A,n = 8),duodenaljejunal bypass (Group B,n = 8),duodenum and total jejunum exclusion (Group C,n = 8),sub-total small intestine exclusion (Group D,n = 8),and sham-operation (Group SO,n=8).All rats were observed for 24 weeks after surgery.Fasting blood glucose was tested at 0,1,3,6,12,and 24 wk.OGTT was performed at 0,1,and 24 wk.After the administration of 3 g/kg glucose by oral gavage,blood glucose was measured in conscious rats before (baseline) and after 30,60,120,and 180 minutes.Areas under curves (AUC) were calculated by trapezoidal integration.Results Rats in group A,B,C and D had lower fasting blood glucose levels and better oral glucose tolerance as shown by lower peak and area-under-thecurve (AUC) glucose values through the entire follow-up period[group B (fasting blood glucose:(9.0±2.4) mmol/L vs.(4.4±1.0) mmol/L;OGTT peak:(20.8±3.1) mmol/L vs.(10.3±2.0) mmol/L;AUC:(2658±417) mmol·min/L vs.(1324±317) mmo·min/L,P ＜0.05 or P ＜0.01].Fasting blood glucose had no differences before 12 wk and significantly increased at 12 (9.1±2.4) mmol/L and 24 wk (10.0±2.3) mmol/L in group SO (preoperative values were (8.1±1.9) mmol/L,P ＜ 0.01.There was no differences in oral glucose tolerance as shown by lower peak and area-under-the-curve (AUC) glucose values between 0 and 1 wk (P＞0.05),while the oral glucose tolerance become worse as shown by the delay of peak,higher peak (25.6±2.0) mmoL/L vs.(21.4±2.7) mmoL/l,and AUC (3422±360) mmol·min/L vs.(2667±377) mmol·min/L.Glucose values in group SO at 24 wk compared to preoperatively (P＜0.01).Rats in group A,B,C,and D had lower fasting blood-glucose and better oral glucose tolerance compared with sham-operated group through the entire follow-up period (P＜0.05 or P＜0.01).Rats in group B had lower fasting blood-glucose and better oral glucose tolerance compared to those in group A while the effect on diabetes control in group B,C and D were similar to one another (P＞0.05).Conclusion Small intestinal exclusion containing proximal gut was effective for glucose control and improving gucose tolerance,duodeno-jejunal bypass may be applicable for diabetes control in clinical setting.

Objective:To investigate the treatment effect of cytosine deaminase gene combined 5-FC thermochemotherapy for colon cancer liver metastasis in Nude Mice. Methods:The models of liver metastasis of colon cancer had been established by injecting human colon cancer cells Lovo into portal vein in 30 BALB/c nude mice,which were divided into transgenic group and non-transgenic group randomly. Transgenic group:virus supernatant was injected into abdominal cavity(0.2 mL/d,for 5 days); non-transgenic group:sodium chloride was injected into abdominal cavity(0.2 mL/d,for 5 days). In both groups 42 ℃ 5-FC was injected into the abdominal cavity (500 mg?kg-1?d-1,for 21 days). After treatment,all mice were sacrificed,and then PCR and RT-PCR were performed to detect the expression of targeted gene in carcinoma. The pathology changes of tumor were observed. Results:The targeted gene were detected in the tumor tissues. The rates of liver metastasis tumors were 26.7%(4/15),100.0%(15/15) in transgenic group and non-transgenic group respectively(P

Objective To investigate the target effect of cytosine deaminase genetherapy combined thermochemotherapy on liver metastasis of colon cancer in nude mice.Methods Plasmid G1CEACDNa were transferred into the human colon cancer LoVo cells by liposomes method.The models of liver metastasis of colon cancer were established by injected LoVo-CEACD into peritonum in 45 BALB/C nude mice,then which were randomly divided into three groups:The control(C)group;the prodrug therapy(PDT)group and the prodrug thermochemotherapy(PDTCT group)and 42 ℃ sodium chloride,38 ℃ 5-FC and 42 ℃ 5-FC were injected into the abdominal cavity [500 mg/(kg?d),for 21 d],respectively.After natural death of all mice,their life span,rate of liver metastasis and number of liver metastasis nodules were surveyed and compared among the three groups.The levels of the expression of target gene were detected by RT-PCR in carcinoma tissue,normal liver,stomach,lung,pancreas,small intestine and large intestine tissues.Pathological features were observed.Apoptotic index(AI)of tumor cells were analyzed in every group respectively.Results The CD gene was detected in the tumor tissues,but not in tissues of normal liver,stomach,lung,pancreas,small intestine and large intestine.In C,PDT and PDTCT group,the life span was(25.80?3.65),(34.27?4.08)and(41.87?3.91)d respectively;the rate of liver metastasis was 100.0%(15/15),40.0%(6/15)and 13.3%(2/15),respectively;the number of liver metastasis nodules was(2.93?1.16),(0.80?1.01)and(0.20?0.56),respectively;and AI of tumor cells was 4.59%,9.87%,17.4%,respectively.The life span of PDT and PDTCT guoup was significantly longer than that of group(P