AIM:To observe the effects of prostaglandin E2 receptor1 (EP1) in neuronal cell death induced by hypoxia/reoxygenation and ischemia/reperfusion. METHODS:The cortical neurocytes of neonatal Wistar rats were cultured for 12 days and exposed to hypoxia/re-oxygenation to establish a hypoxia/re-oxygenation model. Another set of cultured primary neonatal cortical neurocytes of rats were pretreated with 17-pt (an antagonist of EP1),then underwent hypoxia for 3 h,re-oxygenated for 21 h. MTT reagent was added 1 h before measuring the cell viability. Neuron apoptosis was determined by flow cytometry. The protein expression was examined by Western blotting. RESULTS:Compared to the control cells (only underwent hypoxia /re -oxygenation and without any pretreatment),the neurons pretreated with 17-pt and then underwent hypoxia/re-oxygenation showed significantly lower survival rate (P

AIM: To observe the role of peroxysome proliferator activated receptor-?(PPAR-?) and the relationship of cyclooxygenase-2(COX-2) and PPAR-? in injury of cultured rat cortical neurons induced by hypoxia/reoxygenation.METHODS: Primary rat cortical neurons were cultured.Experiments include control group,hypoxia/ reoxygenation group and hypoxia/ reoxygenation with PPAR-? agonist group.Cell viability was surveyed by MTT assay.COX-2 protein expression was measured by Western blotting.RESULTS: Neuron viability raised dramatically in hypoxia/reoxygenation with PPAR-? agonist group,compared with hypoxia/reoxygenation group(P

Objective To study the expression changes of CD54 and CD106 in peripheral blood lymphocyte in patients with congest heart failure. Methods With FCM technique, the levels of CD54 and CD106 in lymphocyte from patients with CHF were measured , and those of patients with hypertension , patients with dilated cardiomyopathy and normal controls were measured at the same time. Cardiac function during heart failure episodes and remission stage was monitored by Color Doppler Echocardiography. Results Levels of CD54 and CD106 were significantly elevated in patients with hypertension , patients with active CHF and hypertension , patients with inactive CHF and hypertension when compared with those of normal controls. Levels of CD54 and CD106 were significantly elevated in patients of dilated cardiomyopathy , patients with active CHF and dilated cardiomyopathy , patients with inactive CHF and dilated cardiomyopathy when compared with those of normal controls. Levels of CD54 and CD106 in patients of CHF were elevated with the degree of CHF. There was significantly negative correlation between LVEF and CD54 of CHF. Conclusions CD54 and CD106 may use as the marker to monitor the progress of CHF.

Objective:To investigate the ultrasonographic findings of breast ductal carcinoma in situ and the causes of misdiag-nosis .Methods :Ultrasonographic features of 17 cases with breast ductal carcinoma in situ which were confirmed by operation and pathology were retrospectively reviewed .Results:Among the 17 cases of lesions ,according to the ultrasonic diagnosis there were 14 cases of malignant tumor ,2 cases of benign lesions ,and 1 case with no mass .Diagnostic coincidence rate was 82 . 3% by ultrasound .According to the characteristics from literatures and lesions in this study ,17 cases of lesions were roughly classified into 5 categories :solid mass(6 cases) ,conduit ectasia(5 cases) ,architecture disorder(4 cases) ,cystic mass(1 case) , other type(1 case) .Conclusions :The ultrasonographic findings of breast ductal carcinoma in situ are varied .Fully understand-ing the ultrasonographic features of breast ductal carcinoma in situ which can decrease missed-diagnosis ,combined with mam-mography examination and breast duct endoscopy which can reduce misdiagnosis ,will improve the diagnostic coincidence rate .

Objective To screen the main environmental factors affecting the growth of Prunella vulgaris L.based on MaxEnt model and ArcGIS;To predict its potential suitable habitats in China;To provide reference for the artificial cultivation of Prunella vulgaris L.Methods Totally 346 pieces of sample point data of Prunella vulgaris L.were collected.Combined with data of 38 ecological factors,the main environmental factors affecting the distribution of Prunella vulgaris L.were screened using MaxEnt model.ArcGIS software was used to evaluate the habitat suitability of Prunella vulgaris L.and analyze the distribution of suitable areas for Prunella vulgaris L.worldwide and in China.Results The main ecological factors affecting the distribution of Prunella vulgaris L.were upper(0-30 cm)soil gravel volume percentage,soil effective water content,upper(0-30 cm)soil exchangeable sodium salt,specific soil types related to agricultural use in soil units and upper(0-30 cm)soil sand content.The highly suitable areas of Prunella vulgaris L.were mainly in Yunnan,Heilongjiang,eastern Inner Mongolia and central Sichuan.Conclusion The predicted results can provide a reference for the introduction of cultivation and sustainable resource utilization of Prunella vulgaris L.