Objective: To study the effect of low-dose dopamine on splanchnic DO_2/VO_2.in rabbit SMAO shock Method:Twenty rabbits were randomly devided into two groups:group A with dopamine,group B as control group. In group A dopamlne was infused at a rate of 5?g?kg~(-1)?min~(-1). SMAO shock model was induced in both groups. Cardiac output index(Cl),portal venous blood flow index (QpvI),mean arterial pressure (MAP),arterial. artiaI and portal venous blood gas analysis.arterial and portal venous serum lactate concentrations(ALT,PLT), total oxygen delivery/oxygen consumption (TDO_2/TVO_2), splanchnic oxygen delivery/oxygen consumption (SDO_2/SVO_2). oxygen extraction ratio(O_2ER) were measured respectively. Result: QpvI, SDO_2,SVO_2 were higher in group A than those of group B (P0.05). CI,MAP, TDO_2/TVO_2 decreased. TO_2ER and SO_2ER increased in both of groups during shock, but without significant differences between both groups. Conclusion: Low-dose dopamine has beneficial effects by increasing portal venous blood perfusion and improving splanchnic oxygenation during SMAO shock in rabbits. but no effects on systemic oxygenation of shock.

Objective We aimed to investigate the evaluation of graduate nursing education by students and faculty in China.Methods Evaluation questionnaire was built based on literature review and interviews with participants and was finally indentified after expert consultation.120 graduate students and 36 faculty members from five nursing schools nationwide were investigated.Results The overall evaluation by faculty members and students was positive.Compared with graduate students,the evaluation by faculty tended to be more negative.Conclusions Nursing schools should put more effort on the quality of education program,facilitate the faculty education,provide abundant resources and build a firm foundation for the development of graduate nursing students.

Objective To reveal the present situation of geriatric nursing, summarize the important research topics, in order to determine the focus of the research and work of geriatric nursing in China. Methods To take geriatric nursing belonged to MeSH thesaurus as key words, related literatures from 2006 to 2016 in Web of Science core collection were searched, then clustering analysis were adopted to analyze highly cited papers. Results Totally 2148 pieces of literatures related to geriatric nursing, there were 80079 pieces of quotations. The contents involved inappropriate medication use and falls in geriatric nursing risk management, functional decline, delirium and chronic cognitive impairment of the elderly, distinguishing between the basic theory of geriatric diseases, tools and practice of geriatric nursing assessment. Conclusions The current research hotspots on geriatric nursing includes: the risk management of geriatric nursing, prevention and control of geriatric syndrome, systematic study on geriatric nursing assessment.

Objective To investigate the signal pathways involved in the effects of thyroid stimulating antibody(TSAb)on the secretory function of cultured thyrocytes.Methods (1)With the method of enzyme-linked immunosorbent assay (ELISA),the PKA and PKC activities were observed.(2)Inhibitors or activators of protein kinases (PKA and PKC) were used to activate or block the signal pathways and the secretion of T3 measured by radioimmunoassay.Results (1)TSAb activated both PKA and PKC in thyrocytes in a dose-,time-dependent manner(.P.

Objective To set up a method for determining the quercetin in Pyrola calliatha H.Andres.Method HPLC method was carried on Agilent Eclipse XDB-C18 column(4.6 mm?150 mm,5 ?m) with mobile phase of methanol-0.1% phosphoric acid(50:50),and the detecting wavelength was at 370 nm.Result The standard curve was linear within the range of 0.092～0.46 ?g.The average recovery rate was 99.24% and RSD was 0.70%.Conclusion The method is simple,accurate,highly sensitive and reproducible.It can be used for the quality determination of quercetin in Pyrola calliatha H.Andres.

Objective To investigate diagnosis and surgical treatment of primary retroperitoneal tumor. Methods The clinical data of 26 cases with primary retroperitoneal tumor was retrospectively analyzed.Results 13 cases with benign tumor and 10 cases with malignant tumor underwent total resection, only 3 cases with malignant tumor underwent local resection. One case with malignant tumor recurred within 2 weeks and death after operation about 28 days.Conclusions IVP,B-ultrasonography, CT and MR diagnosis of primary retroperitoneal tumor is very important for preparation of preoperation and estimation of target organ. Repeatedly operation can improve cure rate.

Objective To study the perioperation management and surgical therapy of Graves' disease accompanied with severe thyroid gland enlargement. Methods 32 cases of Graves' disease with severe thyroid gland enlargement were included in our study and their symptom control, perioperation management, surgical therapy and postoperative complication treatment were analysed retrospectively. Results There was no surgical death among the 32 patients. The incidence of postoperative complications was 21.9%. And among them, 2 cases had respiratory tract obstruction, 2 cases temporary hypocalcemia and 3 cases mild hypothyroid. ConclusionFor Graves' disease, surgical therapy has a satisfactory effect but a higher incidence of complication.

Objective To investigate the effect of one-stage total hip arthroplasty (THA) for advanced active tuberculosis of the hip.Methods Data of 19 patients who had received one-stage THA for advanced active tuberculosis of the hip in our hospital from September 1998 to October 2010 were retrospectively analyzed.There were 14 males (15 hips) and 5 females (5 hips),aged from 21 to 74 years (average,44.1 years).According to the clinicoradiologic classification of tuberculosis of the hip introduced by Babhulkar and Pande,there were 3 cases of grade Ⅲ and 16 cases of grade Ⅳ.The average time interval from onset of symptoms to THA was 15.1 weeks (range,5-26 weeks).Chest radiographs showed old pulmonary tuberculosis in 3 patients.One patient had a sinus tract in hip skin.The diagnosis was confirmed by histopathology in all patients.The antituberculous medication was applied for at least 2 weeks preoperatively and 9 to 12 months postoperatively.Results All patients were followed up for 12 to 142 months (average,57 months).Dislocation of the hip occurred in 1 patient 6 days after operation,which was cured by manual reduction.Tuberculosis recurrence occurred in 1 patient 5 months after operation,which was cured after debridement without removing component and antituberculous medication for 1 year.Cup loosening was found in 1 patient 9 years after operation.No reactivation of infection or implant loosening was found in other patients.The average Harris score improved from preoperative 35 (range,27-46) to 90 (range,65-96) at final follow-up.Conclusion One-stage THA can be safely performed in advanced active tuberculosis of the hip,which can relief symptoms and improve hip function.

Objective:To apply two dimensional gel electrophoresis(2-DE) to initially analyze the protein expression in hippocampus of rats with spleen yin deficiency syndrome and to get the theory foundation for investigating the molecular mechanisms of Alzheimer's Disease and spleen yin deficiency syndrome.Methods:Rats model of spleen yin deficiency were set up by combination of improper diet,overstrain and consumption of body fluid.Qualitative changes of the proteome expression in hippocampus were compared between the model group and control group by two-dimensional gel electrophoresis.Results:The model group of spleen yin deficiency had the appearance of spleen yin deficiency,for example,irritability,increment in biting frequency,increase of rectal temperature and drinking water(P

Objective To evaluate ipsilateral hepatic blood flow exclusion in combination with liver hanging manoeuvre for hepatectomy. Methods From Jul 2007 to Feb 2008, 14 cases underwent hemihepatectomy under ipsilateral hepatic blood inflow and draining hepatic vein exclusion in combination with liver hanging manoeuvre for liver malignancier or ligating ligating vessels, portal vein branch and major hepatic vein to set up a tunnel at the interior vena cava. Results Small laceration on fight hepatic vein was encountered in 2 cases during the operation and managed by suturing. There was no intraoperative injury on inferior vena cava or short hepatic veins. Blood loss ranged from 100 ml to 600 ml with a mean of 280 ml. Postoperative pleural effusion was cured conservatively in 3 cases and paracentes was needed in one case. There was no severe postoperative hepatorenal dysfunction, biliary fistula, infection or other major complications. The operative mortality was nil. Conclusion For regular major hepatectomy, ipsilateral hepatic blood flow exclusion plus liver hanging manoeuvre has the advantage of less blood loss and protecting contralateral hemiliver from reporfusion injuries.