Objective: To study the effect of low-dose dopamine on splanchnic DO_2/VO_2.in rabbit SMAO shock Method:Twenty rabbits were randomly devided into two groups:group A with dopamine,group B as control group. In group A dopamlne was infused at a rate of 5?g?kg~(-1)?min~(-1). SMAO shock model was induced in both groups. Cardiac output index(Cl),portal venous blood flow index (QpvI),mean arterial pressure (MAP),arterial. artiaI and portal venous blood gas analysis.arterial and portal venous serum lactate concentrations(ALT,PLT), total oxygen delivery/oxygen consumption (TDO_2/TVO_2), splanchnic oxygen delivery/oxygen consumption (SDO_2/SVO_2). oxygen extraction ratio(O_2ER) were measured respectively. Result: QpvI, SDO_2,SVO_2 were higher in group A than those of group B (P0.05). CI,MAP, TDO_2/TVO_2 decreased. TO_2ER and SO_2ER increased in both of groups during shock, but without significant differences between both groups. Conclusion: Low-dose dopamine has beneficial effects by increasing portal venous blood perfusion and improving splanchnic oxygenation during SMAO shock in rabbits. but no effects on systemic oxygenation of shock.

Objective We aimed to investigate the evaluation of graduate nursing education by students and faculty in China.Methods Evaluation questionnaire was built based on literature review and interviews with participants and was finally indentified after expert consultation.120 graduate students and 36 faculty members from five nursing schools nationwide were investigated.Results The overall evaluation by faculty members and students was positive.Compared with graduate students,the evaluation by faculty tended to be more negative.Conclusions Nursing schools should put more effort on the quality of education program,facilitate the faculty education,provide abundant resources and build a firm foundation for the development of graduate nursing students.

Objective To reveal the present situation of geriatric nursing, summarize the important research topics, in order to determine the focus of the research and work of geriatric nursing in China. Methods To take geriatric nursing belonged to MeSH thesaurus as key words, related literatures from 2006 to 2016 in Web of Science core collection were searched, then clustering analysis were adopted to analyze highly cited papers. Results Totally 2148 pieces of literatures related to geriatric nursing, there were 80079 pieces of quotations. The contents involved inappropriate medication use and falls in geriatric nursing risk management, functional decline, delirium and chronic cognitive impairment of the elderly, distinguishing between the basic theory of geriatric diseases, tools and practice of geriatric nursing assessment. Conclusions The current research hotspots on geriatric nursing includes: the risk management of geriatric nursing, prevention and control of geriatric syndrome, systematic study on geriatric nursing assessment.

Objective To set up a method for determining the quercetin in Pyrola calliatha H.Andres.Method HPLC method was carried on Agilent Eclipse XDB-C18 column(4.6 mm?150 mm,5 ?m) with mobile phase of methanol-0.1% phosphoric acid(50:50),and the detecting wavelength was at 370 nm.Result The standard curve was linear within the range of 0.092～0.46 ?g.The average recovery rate was 99.24% and RSD was 0.70%.Conclusion The method is simple,accurate,highly sensitive and reproducible.It can be used for the quality determination of quercetin in Pyrola calliatha H.Andres.

Objective To investigate the signal pathways involved in the effects of thyroid stimulating antibody(TSAb)on the secretory function of cultured thyrocytes.Methods (1)With the method of enzyme-linked immunosorbent assay (ELISA),the PKA and PKC activities were observed.(2)Inhibitors or activators of protein kinases (PKA and PKC) were used to activate or block the signal pathways and the secretion of T3 measured by radioimmunoassay.Results (1)TSAb activated both PKA and PKC in thyrocytes in a dose-,time-dependent manner(.P.

Objective To investigate diagnosis and surgical treatment of primary retroperitoneal tumor. Methods The clinical data of 26 cases with primary retroperitoneal tumor was retrospectively analyzed.Results 13 cases with benign tumor and 10 cases with malignant tumor underwent total resection, only 3 cases with malignant tumor underwent local resection. One case with malignant tumor recurred within 2 weeks and death after operation about 28 days.Conclusions IVP,B-ultrasonography, CT and MR diagnosis of primary retroperitoneal tumor is very important for preparation of preoperation and estimation of target organ. Repeatedly operation can improve cure rate.

Objective To study the perioperation management and surgical therapy of Graves' disease accompanied with severe thyroid gland enlargement. Methods 32 cases of Graves' disease with severe thyroid gland enlargement were included in our study and their symptom control, perioperation management, surgical therapy and postoperative complication treatment were analysed retrospectively. Results There was no surgical death among the 32 patients. The incidence of postoperative complications was 21.9%. And among them, 2 cases had respiratory tract obstruction, 2 cases temporary hypocalcemia and 3 cases mild hypothyroid. ConclusionFor Graves' disease, surgical therapy has a satisfactory effect but a higher incidence of complication.

Objective To explore the relationship and clinical significance between the serum levels of oxidized low density lipoprotein (Ox-LDL) and soluble P-selectin (SP-S),and carotid atherosclerosis (CA) in metabolic syndrome (MS) patients.Methods In 103 cases of MS patients and 44 healthy subjects (control group),in MS patients with 3 MS diagnostic criteria for the low risk group (51 cases),with ≥ 4 MS diagnostic criteria for high risk group (52 cases).Carotid artery intima-media thickness (IMT) and plaque was measured by color Doppler ultrasound,serum Ox-LDL,SP-S was determined by enzyme linked immunosorbent assay,and pearson regression analysis of linear correlation and classification of two non condition Logistic was performed.Results The serum levels of Ox-LDL,SP-S,carotid artery IMT in low risk group and high risk group were higher than those in control group [(36.42 ±7.17) μg/L and (54.44± 8.10) μg/Lvs.(30.08±7.15) μg/L,(71.46± 12.52) μ g/Land (89.33±20.28) μg/Lvs.(49.95 ± 12.49) μ g/L,(0.86 ± 0.11) mm and (1.00 ± 0.10) mm vs.(0.69 ± 0.11) mm],the difference was statistically significant (P ＜ 0.01).The incidence of plaque in high-risk group and low-risk group was significantly higher than that in control group [55.8％ (29/52) and 45.1％ (23/51) vs.15.9％ (7/44)],the difference was statistically significant (P ＜ 0.01).Linear correlation analysis results of Pearson display,Ox-LDL was positively correlated with SP-S (P ＜0.01).Conclusion Serum Ox-LDL and SP-S are independent risk factor for carotid atherosclerosis in MS patients,they can evaluate progression of carotid atherosclerosis in patients with MS.

Objective:To apply two dimensional gel electrophoresis(2-DE) to initially analyze the protein expression in hippocampus of rats with spleen yin deficiency syndrome and to get the theory foundation for investigating the molecular mechanisms of Alzheimer's Disease and spleen yin deficiency syndrome.Methods:Rats model of spleen yin deficiency were set up by combination of improper diet,overstrain and consumption of body fluid.Qualitative changes of the proteome expression in hippocampus were compared between the model group and control group by two-dimensional gel electrophoresis.Results:The model group of spleen yin deficiency had the appearance of spleen yin deficiency,for example,irritability,increment in biting frequency,increase of rectal temperature and drinking water(P

Objective To study the clinic features and the mechanism of skin damage induced by high-dose methotrexate(HD-MTX).Method Children treated with HD-MTX were enrolled in a retrospective study in which the incidence and presentations of skin damage were concluded.Mechanism of the damage was studied base on skin pathology of an animal model.Result Skin damage presented in 3～9(median 5) days after HD-MTX and usually followed by bullation and exfoliation.Twelve cases,more severe than the others,accompanied with fever and symptoms of other organs especially the respiratory tract and the intestine.Skin damage was reduced when large dose rehydration used before,during and after HD-MTX.Conclusion HD-MTX may cause scald-like skin damage directly by its prolonged toxic effect on epidermal cells.Besides extended tetrahydrofolic acid rescue and supportive treatments,anti infection is the most important in dealing this condition.