1.Determination of Isorhamnetin-3-O-nehesperidine in Shenghua Tablets by RP-HPLC
International Journal of Traditional Chinese Medicine 2012;34(3):224-226
Objective To establish a HPLC method for the determination of Isorhamnetin3-O-nehesperidinein Shenghua Tablets.Methods The RP-HPLC system was consisted of the Luna-C18(4.6 mm×250 mm,5 μm) column,the mobile phase was acetonitrile -0.4%H3PO4 (10 ∶ 90),the flow rate was set at 0.8 ml/min,the column temperature was 30*℃,and the UV detector was 254 nm.Results The linear response range was 0.056~ 1.12 mg/ml (r=0.9997).The average recovery of mangiferin was 98.0%,and RSD<2%.Conclusion The method was simple,rapid,accurate and repeatable.It could be applied in the determination of Isorhamnetin-3-O-nehesperidine in Shenghua Tablets.
2.The comparison of two methods of detecting pepsinogen
Lianying ZHANG ; Hu XIAO ; Xu LU ; Linlin XIAO
International Journal of Laboratory Medicine 2016;37(20):2810-2812
Objective To investigate colloidal gold immune chromatography (GICA ) and enzyme linked immunosorbent assay (ELISA) detection pepsinogen (PG) consistency of results ,so as to provide experimental evidence for the use of colloidal gold im‐munochromatography assay PG and briefly analysis of its clinical utility .Methods Totally 40 cases in our hospital digested samples and medical centers 60 healthy population sample in Endoscopy Center by endoscopy diagnosed with inflammation of the stomach disease ,respectively ,with colloidal gold immune chromatography and enzyme‐linked immunosorbent assay PG ,colloidal gold immu‐noassay chromatography method to be evaluated ,as the reference ELISA method .The results were compared to the method and bias analysis .Results GICA method with a correlation coefficient ELISA test results were greater than 0 .95 ,indicating a good correla‐tion between the two methods and there is no overall bias;sensitivity and specificity of the two methods were (0 .775 ,0 .85) and (0 .782 ,0 .823) .Conclusion Both GICA and ELISA can be used for screening or diagnosis of stomach illnesses .
3.Determination aristolochic acid A inPaishi granule by SPE-HPLC
Xuehua DENG ; Hongling WU ; Lianying LU ; Zhihao LI ; Zhaoyu CHEN
International Journal of Traditional Chinese Medicine 2015;37(9):822-825
Objective To establish a method for the determination of aristolochic acid A inPaishi granule.Methods The HPLC system consisted of the Phenomenex Luna C18(4.6 mm×250 mm, 5μm)column, the mobile phase consisted of acetonitrile and 0.01% HAc, gradient elution flow rate was 1.0 ml/min, the column temperature was 35℃, The UV detector was set at 250 nm.Results The linear response range was 0.029-0.580μg/ml (r=0.999 9). The detection limit and quantitation limit of aristolochic acid A inPaishi granule were 0.9 and 3.0 ng/ml. The average recovery of aristolochic acid A was 96.4%.Conclusion The method is high sensitivity, accurate, repeatable and high specificity,and can be used as an inspection method for safe use of Paishi granule.
4.Curriculum of introduction to clinical medicine in the University of Ottawa's Faculty of Medicine and its reference
Xia LIANG ; Xiaodong ZHU ; Li LI ; Changyuan WEI ; Lianying GE ; Xianzhong BAI ; Na Huang ; Xiaoxing LU ; Xiuning LI ; Chao GAO ; Hannan LIU ; Yong TANG
Chinese Journal of Medical Education Research 2014;(2):120-124
Curriculum of introduction to clinical medicine(ICM) in the University of Ottawa's Faculty of Medicine was analyzed. Characteristics of ICM course in the University of Ottawa's Faculty of Medicine were: early setting, rich in content, long duration and focusing on clinical. ICM course between Guangxi Medical University and University of Ottawa's Faculty of Medicine were compared. Taking advantages of ICM course in the University of Ottawa's Faculty of Medicine was conductive to better understanding the importance , teaching objectives and means of ICM course . The teaching quality evaluation system of ICM course would be built in the future.
5.Study on the construction of competency of middle-level cadre in functional department of research institute
Lianying MIAO ; Tingting JI ; Li LU ; Yongsi HAO ; Panhe LIU ; Peng ZHENG ; Yongjun CHEN
China Medical Equipment 2024;21(2):162-165
Objective:To analyze the current situation of middle-level cadres in functional departments of scientific research institutes and to construct a job competency model for middle-level cadre.Methods:A cluster random sampling method was used to conduct a questionnaire survey in March 2022 on the satisfaction level of middle-level cadres in the functional departments of the Institute of Blood Transfusion of the Chinese Academy of Medical Sciences,a mid-level cadre competency questionnaire survey was conducted to collect data among functional department personnel of scientific research institutes affiliated to the Chinese Academy of Medical Sciences and Peking Union Medical College.The principal component analysis method was used to extract factors,and the maximum variance rotation method was used to conduct exploratory factor analysis to construct a competency model.Results:The Middle-level cadres in scientific research institutes had problems such as old age,traditional management style,etc.The competency model of middle-level cadres in functional departments of scientific research institutes included 3 main competencies:responsibility taking ability,communication ability and learning execution ability,and 34 competency factors.Conclusion:The competency model of middle-level cadres in functional departments of scientific research institutes can provide basis and reference for the selection and appointment,education and training,performance management and career planning of middle-level cadres in scientific research institutes.