0.05).The serum estradiol level of rat was(22.13?3.54)ng/L.Conclusion Those results showed that estrogen and its receptors has an important role in adjusting conjunctiva epithelium and the basal cell in the deep part of Henle gland,while they do not directly adjust the secretion function of the goblet cell of Henle gland.

0.05).It has statistically significant difference in PR expression between the age groups studied(P0.05),AR expression in Zeis glands between the age groups studied(P0.05).Conclusion Meibomian glands and Zeis glands in fetus of 4 months expressed in ER,PR,AR;ER and AR are expressed from children to adult,mainly in ER.All of receptors are located on membrane,in plasma and nuclear as well.

Objective To explore the localization and distribution of estrogen receptor alpha (ER ?) and estrogen receptor beta (ER ?) in retina tissues of normal female rats. Methods Twenty-two SD female rats were killed by dislocation of cervical vertebra, the eyeballs were paraffin embedded and made to a series of sections which were then studied by means of immunohistochemical method. The uterus samples of rats with positive ER were used as positive control, and PBS as negative control. The serum estradilum levels of rats were examined by radioimmunoassay. Results A few specimens were found to be ER ? positive expression in retina pigment epithelium (RPE), ganglion cell layer and nerve fiber layer; while in other regions the ER ? negative expressed. The ER ? evidently expressed in different retina regions which were detected mainly in RPE and nerve ganglion cell layer as well as nerve fiber layer, and secondly in internal plexiform layer, while few ER ? expressed in other regions. Immunoreactivity positive substances were granule in shape which were located in the cytoplasm of pigment epithelium, or in cytoplasm, or in the nucleus of ganglion cell, or diffuse distributed in relative regions of retina. The positive expression rates of ER ? were higher than that of ER ? in RPE, ganglion cell layer and in nerve fiber layer (P

Objective To investigate the expression and clinical significance of the estrogen receptor(ER) subsets ER?,ER? and Ki-67 in tissues of different thyroid diseases.Methods The pathological data of 93 patients with thyroid diseases were collected from Jun.2003 to Jun.2007,including thyroid carcinoma(56 cases),thyroid follicular adenoma(13 cases),nodular goiter(14 cases) and normal thyroid tissue(10 cases).S-P immunohistochemistry was performed to determine the expressions of ER?,ER? and Ki-67 in the thyroid tissues.Results ER? and ER? were expressed in the plasma and nuclei of thyroid epithelial cells,and ER? was also expressed in the stroma.The positive rates of ER? expression in cases with thyroid carcinoma,thyroid follicular adenoma,nodular goiter and normal thyroid tissue was 87.5%,69.2%,42.9% and 30.0%,respectively,with significant difference between carcinoma and goiter or normal thyroid tissue(P0.05).Conclusion ER? and ER? may play an aggravating effect on cell proliferation in the disease course of the thyroid carcinoma.The expressions of ER? and Ki-67 are related to invasion and metastasis of thyroid carcinoma.

Objective To compare the reliability of erythrocyte parameter mean corpuscular volume(MCV)and mean corpuscu-lar hemoglobin(MCH)for screening thalassaemia trait.Methods A fresh venous blood sample with the cut-off value of MCV was sent to 21 hospitals of Zhuhai city for conduct the full blood cell analysis within 1 d.Then the inter-and intra-coefficient of varia-tion (CV)as well as bias values of MCV and MCH were calculated and compared.In addition,10 EDTA-anticoagulant venous blood samples were divided into two parts,the effects of stored temperature and time on MCV and MCH were observed.Results The coefficient of variation(CV)of MCV (4.1%)was significantly greater than that of MCH (2.8%)among 21 laboratories,the qualification rate of MCH detection results was 100%,which was significantly higher than 66.7% of MCV (P <0.05).When the whole blood samples were stored under 2 kinds of the temperature condition(room temperature and refrigeration)for 72 h,MCH changed little (P >0.05).When these samples were stored under the refrigerated condition for 72 h,MCV had no statistically sig-nificant difference compared with the instant detection results of MCV (P >0.05),when stored at the room temperature for 48 h, MCV was significantly increased (P <0.05),MCV had statistical difference in storage for 48 h between the room temperature and the refrigeration.Conclusion Among laboratories and under different temperature conditions,the reproducibility of MCH is better than that of MCV and is more stable than MCV.MCH as the clinical first-line screening for thalassaemia is more reliable than MCV.

Objective To explore distribution of the estrogen receptor alpha(ER?)and estrogen receptor beta(ER?)in meibomian gland tissue of the normal female rats.Methods 22 SD female rats were killed by dislocation of cervical vertebra,the tarsal plate tissues of the upper eyelids were in paraffin imbedding and made to a series of sections which were then studied by means of immunohistochemical method,and the uterus of rats was used as positive control and PBS as negative control.The serum estradiol level of rats was detected by radioimmunoassay.Results ER? and ER? were expressed in acinar epithelia of meibomian glands.Among 22 rats,21 rats(95.4%) were found to be ER? positive expression in the basa cell of the meibomian gland acinar,15 rats(68.2%) were found to be ER? positive expression in transitional cell and mature cell of the meibomian gland acinar.14 rats(63.6%) were found to be ER? positive expression in the basa cell of the meibomian gland acinar,8 rats(36.4%) were found to be ER? positive expression in transitional cell and mature cell of the meibomian gland acinar.The positive staining rate for ER? was higher than that for ER?,which have statistically significant difference(P

Objective To evaluate the clinical diagnostic criteria of latent autoimmune diabetes of adults(LADA).Methods A total of 268 subjects consisted of 168 patients with type 2 diabetes(T2DM) and of 100 normal controls were involved in the study.The body mass index(BMI) was calculated by formula(kg/m~2) and the levels of fasting blood glucose(FBG),postprandial blood glucose(PBG),fasting C-peptide(FC-P),postprandial Cpeptide(PC-P),GAD antibody(GADA),ICA,IAA and IA-2 antibody were detected. The patients with positive antibody in T2DM group were considered as LADA and the others in T2DM group still as T2DM.Results The GADA,ICA,IA-2 antibody and IAA antibody were detected in 21.18%,14.29%,1.19% and 0.60% of patients with T2DM,respectively.The indexes such as the age of onset ≤ 40 years old,overt symptom,ketosis,without chronic complication and hypertension,BMI90.00%).Conclusion The GADA is the most specific antibody in the diagnosis of patients with autoimmune diabetes.The indexes such as the age of onset≤40 years,overt symptom,ketosis,without chronic complication and hypertension,BMI

Objective Data mining method was used to analyze gynecological clinical medication rule of Cai Lianxiang who is a famous veteran TCM doctor. Methods A total of 165 cases and 337 visits of Cai Lianxiang were selected to establish a database by excel, and were analyzed by TCM diagnosis and treatment data intelligent analyses system. High-frequency herbs and combinations of herbs were analyzed and were used to accord with academic thoughts and clinical experience of Cai Lianxiang. Results Results showed 10 high-frequency herbs and 7 herb combinations, which accorded with academic thoughts of Cai Lianxiang. The pathogenesis of gynecological diseases was defficiency of essence and blood, the inbalance among live, spleen and kidney. Thus, the principles of treatment were enriching essence and nourishing blood, adjusting liver, supplementing kidney and fortifying spleen. Conclusion It is significant that the data mining methods were used to summarize the rules of Cai Lianxiang's gynecological medication.

Objective To analyze clinical differential diagnostics and medication law of CAI Lian-xiang of treating gynecological diseases from theChong-Ren;To provide references for clinical medication. Methods Medical cases of gynecological diseases treated by differential diagnostics from“Chong-Ren”from December 2013 to January 2015 in out-patient department of Xiyuan Hospital of CACMS by CAI Lian-xiang were collected. Excel2007 was used to establish the database, and the frequency and correlation analysis were made on the differentiation, treatment principles, treatment methods, medicine and medical combination by using the large data intelligent analysis system of Chinese medicine diagnosis and treatment. The results were confirmed with CAI Lian-xiang's academic thought and clinical experience. Results Totally 103 medical cases, 277 diagnosis times, 277 prescriptions and 92 kinds of Chinese materiamedica were included. Although different types of gynecological diseases from theChong-Ren treatment of specific treatment were different, all were related to liver, spleen and kidney;prescription contained 22 high-frequency medicines (>50 times), and the first three medicines were Cuscutae Semen 178 times (64.3%), Angelicae Sinensis Radix 167 times (60.3%), Paeonize Radix 158 times (57.0%). The association analysis results showed 9 combination of Tiaochongren medicine combination group, 11 Qiangchongren meidcine combination group, 4 Zichongren medicine combination group, and 4 Guchuanrenmediicne combination group. Conclusion CAI Lian-xiang believes that gynecological diseases are closely related to “Chong-Ren”, and treatment from“Chong-Ren”focuses on the adjustment and nourishment of liver, spleen and kidney. The data miningresults of medication law is accordance with the relevant academic thoughts, which can provide references for clinic.

Objective To research the expression of the estrogen receptor alpha ( Erα) and estrogen receptor beta ( Erβ) in uvea tissues of the female rats, and to provide molecular biology data for further studies of the relation estrogen to uvea diseases. Methods Twenty-two adolescent SD female rats were selected. All rats were killed by dislocation of cervical vertebra, the eyeballs were in paraffin imbedding and made to a series of sections, using immunohistochemical method;Erα and Erβ distribution were investigated in uvea tissue of rats;and quantitied by Tanaka scores analytical method. The uteri of rats was used as positive control and PBS as negative control. The level of estradiol in serum of the rats were examined by radioimmunoassay. Results The expression level of Erβ was moderate or highter in stroma cell, anterior pigment epithelium as well as pasterior pigment epithelium of the iris, unpigmented epithelium, pigmented ciliary epithelium and vascular endocemet of the choroid layers. But Erα was not obviously expressed in uvea tissues. The expression rate of Erβ was higher than Erα in these tissues(P＜0.05). Immnoreactivity positive substance was granule, which was distributed in the cytoplasm or in the nucleus. The level of estradiol in serum of the rats was (22.13±3.54)ng/L.Conclusion The expression of either Erα or Erβ in uvea tissues of rats is mainly in Erβ. The results indicate that uvea tissue is regulated directly by estrogen throught Erβ.