The experimental acute spinal cord injury (SCI) was performed in 10 rabbits at L_1 level using the method of compredssing the abdominal lateral of spinal cord. The rabbits were treated with naloxone (2mg/kg/h) intravenously 45 minutes after SCI and treated continually for 4 hours. In the control group, equal volume of saline was administered. The ascending and descending conductive function of spinal cord was measured by mortor evoked potential (MEP) and cortical somatosensory evoked potential (CSEP). The result showed that amplitude of the MEP and CSEP peaks came to a normal level 2 hours after SCI and were significantly higher (P

Objective To improve the recognition of abnormal image of soft tissue in cheeks. Methods Images of 47 cases with abnormal soft tissue in cheeks were retrospectively studied. Results (1) 15 cases were originated from soft tissue of the cheeks in which 8 cases were benign and 7 cases were malignant.6 cases were invaded by maxillofacial affection nearby,in which 1 case was benign and 5 cases were malignant.26 cases were involved by lesions of jaw bones,including benign tumor of jaw bones in 9, malignant tumor of jaw bones in 14 and chronic osteomyelitis of jaw bones in 3 and all had bony destruction; (2) According to originated position,21 cases were not originated from jaw bones,in which there were 5 cases (23.8%) with destruction of jaw bones;(3)In the 26 cases with abnormal soft tissue of cheeks originated from jaw bones, 9 were benign tumors in which all the soft tissue masses were mainly localized inside of the jaw bones, while 14 cases were malignant tumors in which all the soft-tissue masses were localized both inside and outside of the jaw bones. There was remarkable difference between the two groups(P=0.000).All 9 cases of benign tumors appeared as dilated destruction of jaw bones, while in the 14 cases of malignant tumors, 12 cases showed osteolytic bone destruction and the other 2 cases showed ossific bone destruction,there was significant difference between the two groups(P=0.000).Conclusion CT and MRI are of significance in judging original position, extent and differential diagnosis between benign and malignant lesions of abnormal soft tissue in cheeks.

Objective To study the prevalence of peripheral arterial disease(PAD).Methods Totally 2668 fishermen and their families above 35 years old were recruited in a cross sectional study of randomly cluster sampling in 10 villages of Zhoushan fishery area,one of the biggest four fishery in the world,in Summer.All the cases underwent the ankle arm index(AAI) measurement using portable doppler ultrasound device.An AAI less than or equal to 0.9 was considered to be indicative of significant PAD.Fasting plasma glocose and oral glucose tolerance test(OGTT)were also examined to distinguish normal glucose tolerance(NGT),imparied glucose tolerance(IGT)and diabetes melitus(DM). Results The prevalence of PAD of 2668 cases was 2.1%.PAD prevalence was found to be 2.1%,2.9%,and 6.3% in patients with NGT,IGT,and DM respectively.It was seldom seen in people under 50 years old and the PAD was more popular in older people.Conclusions The prevalence of peripheral arterial discase of the general people in Zhoushan fishery area is lower.

Objective:To observe effects of Cuttle-Bone on blood inorganic phosphorus and parathyroid hormone(PTH)in patients of uremia in hemodialysis.Methods:74 cases of uremia in hemodialysis were randomly divided into a control group,a cuttlefish bone treatment group and a calcium carbonate treatment group.The control group did not take any conjugation agent of phosphorus. The cuttlefish bone treatment group were treated with oral administration of cuttlefish bone powder 3.0g in each meal and the calcium carbonate treatment group with chewing calcium carbonate tablets 1.5g in each meal.They all were treated for 3 months. Results:After treatment,blood inorganic phosphorus and PTH decreased(P0.05),with less effect on blood calcium(P

Objective To explore the function of auditory efferent system in the subjects with tinnitus.Methods Effects of contralateral stimulation on otoacoustic emission in 24 subjects with tinnitus were measured for analysis the function of the medial olivo-cochlea system. Results ①The incidence of SOAE 41.7% in 24 normal ears,and 37.5%,20%,33.3% in different groups of tinnitus and the amplitude were suppressed. ②The suppressive amplitude of TEOAE was 2.1?0.8 dB SPL in 24 normal ears and 1.9?0.8 dB SPL in 16 ears with tinnitus, but there was no suppression in 8 ears with tinnitus. ③There were observable suppression in 3 ears, but 2 ears had no contralateral suppression in tinnitus with normal hearing group. 8 ears showed suppression in tinnitus with hearing loss, 2 ears had no changes. 4 ears had no contralateral suppression in tinnitus subjects with low frequency hearing loss.Conclusion The effects of suppression in the normal ears of subjects with tinnitus is as normal subjects, but those different results of suppression in the tinnitus ears indicate that mechanism of tinnitus is multiformity.

Objective To investigate the BUN content and ALT activity in cataract at serum and Lens by Fuming tablets.Methods The BUN content in serum and cens of the model group,treatment and control groups was detected by Urease-Berthelot method,while ALT activity in serum and Lens of the model,freatment and control group was delected by Laishi method.Results The BUN contemtin lens of treatment group Ⅰ and Ⅱ was lower than that in control and molel group(P

Objective To explore the transmission route of Legionella pneumophila in central air-conditioning system. Methods The environmental samples collected from four public places of Nanjing city were detected by nested-PCR and analyzed by phylogenetic analysis. Results The positive rate of Legionella pneumophila in sediment, cooling water, aerosol near cooling tower, dust in duct surface and indoor air were 100%(9/9),78.6% (11/14),25.4% (17/67),16.7% (2/12) and 17.3% (13/75), respectively. The nucleotide sequence similarities of Legionella pneumophila in cooling water and indoor air was more than 92%. Conclusion The main pollution source of Legionella pneumophila is cooling water and Legionella pneumophila can be transmitted to indoor air through central airconditioning system.

Objective To explore the relationship between the post-thoracotomic wound infection and nutritional status.Methods The patients treated with thoracotomy were divided into the simple wound infection group(n=15) as the experimental group and the phase Ⅰ healed group (n=15) as the control group.On the fifth day after operation, the sero-albumin quantity and cumulative nitrogen balance were recorded respectively. The statistical data were given the test.Results Every average index of sero-albumin in the experimental group was lower than the control group. Among them, the total protein comparison had statistical significance (P0 05) and transferrin comparison had statistical difference (P

Objective To investigate the distribution of acquired carbapenemases in carbapenemresistant strains of Klebsiella pneumoniae, and explore its role in epidemiology of nosocomial infection. Methods From November 2008 to March 2009, twenty clinical isolates of carbapenem-resistant Klebsiella pneumoniae were collected from children hospitalized in Wuhan children's hospital. MICs of antibiotics were tested by DNA of Klebsiella pneumoniae. Modified Hodge test was used to screen strains producing carbapenemases,combined imipenem(IPM)-EDTA , meropenem(MEM)- EDTA and ceftazidime(CAZ) - EDTA double-disk synergy test (DDST) were used to detect metallo-β-lactamase-producing. PCR amplification of the carbapenemase and integrase genes, and sequencing were performed. Plasmid conjugation transfer experiments and Southern hybridization were applied to study the mode of drug resistance transmission. Results Four types of Klebsiella pneumoniae were detected by PFGE, type A consisted of 5 strains, including 3 strains of type Al and 2 strains of type A2), type B (2 strains), type C (12strains) and type D (1 strain). Type A and C were the main drug resistant clones. Eight strains of Klebsiella pneumoniae carried both KPC-2 and IMP-4 genes, 10 strains carried IMP-4 gene, 2 strains carried KPC-2 gene. None of NDM-1 ,GIM, SPM, SIM, OXA-23, and VIM carbapenemase genes was detected in 20 isolates. All of 20 isolates carried lntl which were found to be located on bacterial chromosome by Southern blot. Conclusions KPC-2and IMP-4 genes are the major carbapenemase genes in Klebsiella pneumoniae isolated in Wuhan.Transmission of drug resistance is mainly through vertical transmission of type C resistant clone and horizontal transmission of Intl on bacteria chromosome.

Objective To investigate the molecular epidemiology of CRAB isolated from children in wuhan. Methods Forty non-repetitive strains of CRAB were collected from hospitalized children of emergency department, neonatal medicine, cardiothoracic surgery, bone surgery, respiratory medicine and renal medicine in Wuhan children's hospital during December 2008 and May 2009. MIC values were PFGE; KPC, IMP, GIM, SPM, SIM, OXA-23, VIM genes and integrase gene were amplified by PCR and then sequenced to confirm the genotypes.; Plasmid conjugation experiment was used to study the transfer method of bacterial resistance and southern blot hybridization was used to target the resistance genes. Results Susceptible rates of 40 strains to gentamicin, tobramycin, amikacin, ciprofloxacin, levofloxacin, trimoxazole were 20%, 5%, 93%, 93%, 95%, and 23% respectively. Eleven types of clone were detected by PFGE,including 29 strains of type A clone, 2 strains of type B clone, and 1 strain for each type of C to K clone. Eleven isolates produced both IMP-4 and OXA-23 carbapemase. Twenty-six isolates only possessed OXA-23 carbapemase. Thirty-six strains carried class Ⅰ integron. The results of southern blot hybridization showed that Intl, IMP-4 and OXA-23 type were located on chromosome. Conclusions Type A clone of CRAB is the most common. OXA-23 and IMP-4 type are the major acquired carbapemases, especially the OXA-23 is the most common type. The horizontal transmission of OXA-23 and IMP-4 gene mediated by Int1 and the spread of type A resistant clone is the major way of the spread of carbapenem-resistant Acinetobacter baumannii in the region.